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- EMDB-68865: Cryo-EM structure of RSC complex from Chaetomium thermophilum -

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Basic information

Entry
Database: EMDB / ID: EMD-68865
TitleCryo-EM structure of RSC complex from Chaetomium thermophilum
Map dataMain map
Sample
  • Complex: RSC
    • Protein or peptide: WD40 repeat-containing protein
    • Protein or peptide: Ssr1
    • Protein or peptide: DM2 domain-containing protein
    • Protein or peptide: DUF1750-domain-containing protein
    • Protein or peptide: Putative chromatin structure-remodeling complex protein
    • Protein or peptide: Putative chromatin structure-remodeling complex protein
    • Protein or peptide: Rsc7
    • Protein or peptide: Rsc58
    • Protein or peptide: Rsc1
  • Ligand: ZINC ION
KeywordsSWI/SNF-family chromatin remodeling / RSC / PBAF / DNA BINDING PROTEIN
Function / homology
Function and homology information


RSC-type complex / SWI/SNF complex / nuclear chromosome / helicase activity / transcription elongation by RNA polymerase II / chromatin DNA binding / transcription by RNA polymerase II / histone binding / chromatin organization / chromatin remodeling ...RSC-type complex / SWI/SNF complex / nuclear chromosome / helicase activity / transcription elongation by RNA polymerase II / chromatin DNA binding / transcription by RNA polymerase II / histone binding / chromatin organization / chromatin remodeling / hydrolase activity / chromatin binding / regulation of DNA-templated transcription / positive regulation of DNA-templated transcription / DNA binding / DNA-templated transcription / zinc ion binding / ATP binding / nucleus
Similarity search - Function
: / : / Domain of unknown function (DUF7785) / Domain of unknown function (DUF7877) / SWI/SNF and RSC complexes subunit Ssr4, N-terminal / SWI/SNF and RSC complexes subunit Ssr4, C-terminal / SWI/SNF and RSC complexes subunit Ssr4 N-terminal / SWI/SNF and RSC complexes subunit Ssr4 C-terminal / Rsc8/Ssr1/Ssr2, zinc finger, ZZ-type / : ...: / : / Domain of unknown function (DUF7785) / Domain of unknown function (DUF7877) / SWI/SNF and RSC complexes subunit Ssr4, N-terminal / SWI/SNF and RSC complexes subunit Ssr4, C-terminal / SWI/SNF and RSC complexes subunit Ssr4 N-terminal / SWI/SNF and RSC complexes subunit Ssr4 C-terminal / Rsc8/Ssr1/Ssr2, zinc finger, ZZ-type / : / DNA-binding RFX-type winged-helix domain / Chromatin-remodelling complex, RSC SWI/SNF subunit Rsc7/Swp82 / Chromatin remodelling complex Rsc7/Swp82 subunit / RFX-type winged-helix DNA-binding domain profile. / SWIB domain / SWI complex, BAF60b domains / SMARCC, C-terminal / SWIRM-associated region 1 / SNF5/SMARCB1/INI1 / Remodelling complex subunit Rsc/polybromo / SNF5 / SMARCB1 / INI1 / Glutamine-Leucine-Glutamine, QLQ / QLQ domain profile. / QLQ / Myb-like domain profile. / Snf2-ATP coupling, chromatin remodelling complex / Snf2, ATP coupling domain / Snf2-ATP coupling, chromatin remodelling complex / ARID DNA-binding domain / ARID DNA-binding domain superfamily / ARID/BRIGHT DNA binding domain / ARID domain profile. / BRIGHT, ARID (A/T-rich interaction domain) domain / ARID/BRIGHT DNA binding domain / SWIRM domain / SWIRM domain / SWIRM domain profile. / HSA domain / SWIB/MDM2 domain / SWIB/MDM2 domain / SWIB/MDM2 domain profile. / Helicase/SANT-associated domain / HSA domain profile. / Bromo adjacent homology domain / BAH domain / Bromo adjacent homology (BAH) domain / Bromo adjacent homology (BAH) domain superfamily / BAH domain profile. / SWIB/MDM2 domain superfamily / SANT domain profile. / SANT domain / Myb-like DNA-binding domain / : / SNF2-like, N-terminal domain superfamily / SNF2, N-terminal / SNF2-related domain / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / SANT/Myb domain / Homeobox-like domain superfamily / Helicase conserved C-terminal domain / Zinc finger, NHR/GATA-type / Bromodomain, conserved site / Bromodomain signature. / Bromodomain / bromo domain / Bromodomain / Bromodomain (BrD) profile. / Bromodomain-like superfamily / helicase superfamily c-terminal domain / WD domain, G-beta repeat / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / Armadillo-type fold / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / Winged helix-like DNA-binding domain superfamily / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
DM2 domain-containing protein / Uncharacterized protein / Chromatin structure-remodeling complex protein / Uncharacterized protein / Uncharacterized protein / Chromatin structure-remodeling complex protein / Uncharacterized protein / DUF1750-domain-containing protein / WD40 repeat-containing protein
Similarity search - Component
Biological speciesThermochaetoides thermophila DSM 1495 (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.12 Å
AuthorsMa SS / Liu MD / Shen QT / Chen Y
Funding support China, 1 items
OrganizationGrant numberCountry
Ministry of Science and Technology (MoST, China)2023YFA1800403 China
CitationJournal: Sci Adv / Year: 2026
Title: Structural principles underlying the evolution of SWI/SNF chromatin remodelers
Authors: Ma SS / Liu MD / Xu WC / Wang QM / Wang XM / Huang YG / Li MC / Li CH / Shen QT / Chen Y
History
DepositionFeb 1, 2026-
Header (metadata) releaseAug 12, 2026-
Map releaseAug 12, 2026-
UpdateAug 12, 2026-
Current statusAug 12, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_68865.png

Downloads & links

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Map

FileDownload / File: emd_68865.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMain map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.08 Å/pix.
x 400 pix.
= 432. Å		1.08 Å/pix.
x 400 pix.
= 432. Å		1.08 Å/pix.
x 400 pix.
= 432. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.08 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.11
Minimum - Maximum-0.0018851408 - 1.8867971
Average (Standard dev.)0.0013118682 (±0.027680507)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 432.00003 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Focused refined map for RSC-specific lobe and local...

Fileemd_68865_additional_1.map
AnnotationFocused refined map for RSC-specific lobe and local resolution filtered using deepEMhancer
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : RSC

EntireName: RSC
Components
  • Complex: RSC
    • Protein or peptide: WD40 repeat-containing protein
    • Protein or peptide: Ssr1
    • Protein or peptide: DM2 domain-containing protein
    • Protein or peptide: DUF1750-domain-containing protein
    • Protein or peptide: Putative chromatin structure-remodeling complex protein
    • Protein or peptide: Putative chromatin structure-remodeling complex protein
    • Protein or peptide: Rsc7
    • Protein or peptide: Rsc58
    • Protein or peptide: Rsc1
  • Ligand: ZINC ION

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Supramolecule #1: RSC

SupramoleculeName: RSC / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#9
Details: The RSC complex is overexpressed in 293F cells and purified by affinity chromatography, anion exchange chromatography, Grafix, etc.
Source (natural)Organism: Thermochaetoides thermophila DSM 1495 (fungus)

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Macromolecule #1: WD40 repeat-containing protein

MacromoleculeName: WD40 repeat-containing protein / type: protein_or_peptide / ID: 1
Details: The N-terminal 62aa contains 3xFlag tag, 3C site, and the cloning linker.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila DSM 1495 (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 179.692891 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: GSPQQNKTAA LAQHDEADYK DHDGDYKDHD IDYKDDDDKG GSGGSLEVLF QGPGGSGGSG TMTSVQVPQV PPAVQLPGTA IPANMTQQQ AQEVYKRWRQ MKESGVPPTD PEFIKTQNIL SALQRQSDMR KQMQMQQQMQ LQQQQQQQQQ QQQQQQQQQQ Q QQQVMQNG ...String:
GSPQQNKTAA LAQHDEADYK DHDGDYKDHD IDYKDDDDKG GSGGSLEVLF QGPGGSGGSG TMTSVQVPQV PPAVQLPGTA IPANMTQQQ AQEVYKRWRQ MKESGVPPTD PEFIKTQNIL SALQRQSDMR KQMQMQQQMQ LQQQQQQQQQ QQQQQQQQQQ Q QQQVMQNG ANGVVNGTQF GRVAQPGAAP ASMPPATGAL GASALPAGSA APAQNAAGAS ALPPQLQGHP HFTQAQLNLL MN QIKAFRM LGKNAGVPIQ MQKAIFEHRA RRRQSLGKQE PQPAATSVTP TTSAETPAPD ASKPAVNGPE AIKAAAPATT TAS LSDPQS KTFKTVKSPF DAGFIRKTIS YIDHSRRKNR QLVPGLFPTG IDFEQLRADR EKIIFNRMSQ RYQELKSLPG NLAH WDASK DELVADDTAK RKAIIEMKKL ALYSKQRALR EKIGRQMMHY DNLAMTTNRA QYRRMKKHNV REARITEKLE KQQRD AREN RERKKHIDFL QAIQNHRNEV LNAGAAQRLK MSKLSRHMYN HHFNIEKEEQ KRIERTAKQR LQALKANDEE AYLKLL DQA KDTRITHLLK QTDGFLKQLA ASVREQQRQA AEKYGEPMDI PSSEDEDEDD ENGRSKIDYY AVAHRIKEEV TEQASIL VG GTLKEYQLKG LQWMLSLYNN NLNGILADEM GLGKTIQTIS LITYLIEKKK QNGPYLVIVP LSTLTNWNLE FEKWAPSV T KVVYKGPPNA RKMQQEKIRQ GKFQVLLTTY EYIIKDRPLL SKIKWFHMII DEGHRMKNTN SKLSATIQQY YTTRFRLIL TGTPLQNNLA ELWAMLNFVL PNIFKSAKTF DEWFNTPFAN TGGQDKMELT EEEQILVIRR LHKVLRPFLL RRLKKDVEKD LPDKTEKVI KCKFSALQAR LYKQMVTHQK IIVSDGKGGK TGARGLSNMI MQLRKLCNHP FVFDEVENQM NPQNISNDLL W RTAGKFEL LDRILPKYKA TGHRVLMFFQ MTAIMDIMED YLRYRGLHYL RLDGTTKSED RSELLKLFNA PDSPYFMFLL ST RAGGLGL NLQTADTVII YDSDWNPHQD LQAQDRAHRI GQKNEVRILR LITSNSVEEK ILERARFKLD MDGKVIQAGR FDN KSSETD RDAMLRTLLE TADLAESGEQ EEMDDEELNM VLARNEEELA IFQKIDEERN RDPIYGTAPG CKGVPRLMTE DELP EIYLH EGNPAEEENE VHLGRGARER KQIRYDDGLT EEQWLMAVDD DEDTPEAAAA RKQARREKRE QNKLKRLAML NASME NSPS ASRASTEDVE TPKKRGRKPG SKNQEKRKAE DGDDEPPAKK RRGPQGRPKA VGTSSGGSAL APELRDKLQK ACRRIF DGL MNLAVDDDEP PEKPEGENDD ESEDEGPPKR LIIGPFVKLP PKREWQDYYV IIQNPICMND IQKKIKREEY NSLGDMR KD LDLLVTNCRT FNEETSGICQ DANTIEAYFK EHFAKELAED PDLRALEDPN SPNLVGLNVD GVNSSASSSS AEASGPAT T GESSAAQSSQ PASSTTSTTP APAPAPAAPT GPPRIKLVSS ANKENKGDTN TSANGSAAQT NGGVNGVANG NSEA

UniProtKB: WD40 repeat-containing protein

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Macromolecule #2: Ssr1

MacromoleculeName: Ssr1 / type: protein_or_peptide / ID: 2 / Details: The C-terminal 8aa encode Flag tag / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila DSM 1495 (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 75.831109 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MEEGSVSYGT PTTGASPGAN PILAQPPLPD AGDQSEDIQM SESPAIKQDS TTPAPGAENA AERAQAARED TEMGDAPKNE AGHADATGQ NEGNGETKTK DFIENAAREH LIAQTHAIIL PSYSTWFDMN TIHDIERKAL PEFFNNRNRS KTPAVYKDYR D FMINAYRL ...String:
MEEGSVSYGT PTTGASPGAN PILAQPPLPD AGDQSEDIQM SESPAIKQDS TTPAPGAENA AERAQAARED TEMGDAPKNE AGHADATGQ NEGNGETKTK DFIENAAREH LIAQTHAIIL PSYSTWFDMN TIHDIERKAL PEFFNNRNRS KTPAVYKDYR D FMINAYRL NPTEYLTVTA CRRNLAGDVC AIMRVHAFLE QWGLINYQVD ADQRPSHVGP PFTGHFKIIV DTPRGLQPWQ PA ADPVITE GKPNKDTEAK ASATPAPKTE LNLEVGRNIY EANAKHNKIN KSDKPNGEAS AAATNGVTGA DELPKAPIMK VNC YNCGTD CTRIYYHSPQ SDPNSKVKYD LCSTCYLEGR FPGNQTSAHY TRMENPTYSS TLDRDAPWSD AEILRLLEAL ERYD EDWNQ IAEYVGTRTR EECVLQFLQL DIEDKYLESE RLDAPIGLQM LGSHGGQLPF SQSDNPVMSV VSFLASLADP ASTAA AANK SAEILKQNLR NKLEGGSKTA ETANGKGKEK EGTVSSGDSM DVDNKETGSG NGSSEKLTPS QLANIPLAAM GARASA LAS HEEREMTRLV SAAVNVMLEK LELKLKYFNE MEAMLQAERR ELERARQQLF LDRLTFKRRV REVQEVLRQA AATGGEQ GF RMAQEVLAGA AAPGAGNGMT FQPAPAASAV QPLSAEGPVK SYEADYKDDD DK

UniProtKB: Uncharacterized protein

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Macromolecule #3: DM2 domain-containing protein

MacromoleculeName: DM2 domain-containing protein / type: protein_or_peptide / ID: 3 / Details: The C-terminal 8aa encode Flag tag / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila DSM 1495 (fungus)
Molecular weightTheoretical: 61.496863 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MQQQFRGYGQ HAPTPPRPGS GPHGVPPRRG GIGPMMSAGP HPQAPLSAAQ LNQQHQAQQQ ANHLAKLRSR KPTDKNLPDG VEEIVIDHG YVVDAYRQLR DFERRLDATM TRKRLDIVDS LSRNTKRYKT MRVWISNTVE DQYWQNNSLN VDTFDFSSNL E ASYRLKIE ...String:
MQQQFRGYGQ HAPTPPRPGS GPHGVPPRRG GIGPMMSAGP HPQAPLSAAQ LNQQHQAQQQ ANHLAKLRSR KPTDKNLPDG VEEIVIDHG YVVDAYRQLR DFERRLDATM TRKRLDIVDS LSRNTKRYKT MRVWISNTVE DQYWQNNSLN VDTFDFSSNL E ASYRLKIE GRLLEDEEDP SSDDDEPSAN GGDKMDTDSV PAAKPAKPAK AVPAKHGQRY RFSHFFKALT VEFDKSRVAA AA AAGRETT IEWKKPERTP AGAANLPAMA DFDEITFKRP GDENVNVIIN LYRHEDPERF ELSPELAEIV DMKEATRQEA VMA VWEYIK LNKLQEDEEK RNFRCDDMLK KIIPRDSGFI PHLNEYLTPH LRPLSPIKLP YTIRVDEEFH KNPSPTIYDI RVLV DDPLR TRQSSSPYLP FPSNPSYATT LKEIAALDDQ LATLMQAVAH SKAKHAFLSG MARDPVGFVK GWLSSQKRDL DIILG EANR AGRDGVTGGL GAGGGEEAVT AGDEWRRGGK ESVWASQNAR ESVNVLLAKQ PLVLRDYKDD DDK

UniProtKB: DM2 domain-containing protein

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Macromolecule #4: DUF1750-domain-containing protein

MacromoleculeName: DUF1750-domain-containing protein / type: protein_or_peptide / ID: 4 / Details: The C-terminal 8aa encode Flag tag / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila DSM 1495 (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 81.947281 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAVVDPSQNV HGDLLPHIHL ISTFRYATMP RVELQEVTKW LLQAPKIARD TSPFYWTYLD CPADGSILLT WQPTARRGTE FATDGYIWA GPEVQFQQNM GNGLLLEIYF QRAGYRMGEQ YAVHSRRRFR LLLAPGQAPN PNLQVDPNLW IVHYGPAEKN D RLPVSAIV ...String:
MAVVDPSQNV HGDLLPHIHL ISTFRYATMP RVELQEVTKW LLQAPKIARD TSPFYWTYLD CPADGSILLT WQPTARRGTE FATDGYIWA GPEVQFQQNM GNGLLLEIYF QRAGYRMGEQ YAVHSRRRFR LLLAPGQAPN PNLQVDPNLW IVHYGPAEKN D RLPVSAIV LPPPIHQLLN FRQHLLSMGQ IIRKEFMLSD RVNWPQIPFP TRATSMYAPP IPQRHIPQAM AYPPHPGQPI AP APKRRGG QSSSSSSAAA AAAAAAAQAA QMAGAAAGYH PDVFNDEEDV SAGDMFDHLT PRELSLARYQ QNHEWMEEIL LSP YQIGQI EVSDLGLGKK GELAALTEGI FEAQGFDALQ RGPKKPYTGK LDPGLADEFR KRVEERNAAV KAEIEEMKAK HEKM MAKFK RSAVIKQAEQ ELRFAVQETG TEFWRLEGKI DEGEEAPSQK IITKSLDEIV ARVEELVGKH ATVVKDVHRI QDGGY QEPQ PEPPAPTAAP SAGAVGDMSC QASHAGSNGS GVLIGSDHDI DMGGTAGSLL DQMHSSAGFS STSTPMNNFP TPQAHL PVS SIPSSAATPG VVSVSSPNPV PPTAGVPAAQ VQQQQDVQMG GTEPAAVVPA KEATTATEQG ISSGEGDWVV VGKEGIP PA PGPSNITQPP AAPATAPLAT TPAATGPGGA PTPGSTALIE TDFADLDTAG DALASYEAAA AGSGSQNGDD HEGGRLED E EDMDMMVGGE ESAFGEAFEG VEAANAAVGQ GGVEGGGDVS DYKDDDDK

UniProtKB: DUF1750-domain-containing protein

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Macromolecule #5: Putative chromatin structure-remodeling complex protein

MacromoleculeName: Putative chromatin structure-remodeling complex protein
type: protein_or_peptide / ID: 5
Details: The fragment of Rsc9 used for purification was 270-1025aa and a start codon added to its N terminus. The ARID domain is truncated and 6xHis tag were added to its C terminal.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila DSM 1495 (fungus)
Molecular weightTheoretical: 86.517656 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MPPPIQQVPL RIVDTPASNP ELFARKQRQL RYPQPSAPNP GALVRATLGA TALEGPNIYE RCLLALRSSL RAEQAFALNH LVKISYERG DKYKFSQFSG LAEGLTEFAL GVTKLFYGVE MFISHDFDLD CDDPTALDGI NGTDDLDRRL AEVKKVNPDI Q DSIQPAEF ...String:
MPPPIQQVPL RIVDTPASNP ELFARKQRQL RYPQPSAPNP GALVRATLGA TALEGPNIYE RCLLALRSSL RAEQAFALNH LVKISYERG DKYKFSQFSG LAEGLTEFAL GVTKLFYGVE MFISHDFDLD CDDPTALDGI NGTDDLDRRL AEVKKVNPDI Q DSIQPAEF ADQLTLIIEA VLTIRNMVML PDNAWFLSEY PPVKDLLVII LHLPEMDMLV ELKHLALEIA EQLTPYMLLS ES DPLYFIM MRTLERSEDR GMILSALRAL GRIAMQHPTE TNKLKGVGQH PNILPKMASW LLLNDDELLD ACLDFLYQYT AEP DNIDLM LRTINVEHVV AHLVRLLSHG AKRSQREIVL KAAQVAYEAA PEQILALPKD LQERLLAMEE PDRCQAWLRC LFEE DPGSH VTQIAIWQAY NGAFAEALKM QGRPMIGAQE YIKNISNVYQ SGGAQVVREQ GPTGEIQKFI MSGIRPRTRP IALDG RSYL KCHWKYDGPT PPGIPVPQHC GSWHLTAETM WRHILVDHLR EPITSDGKFQ NREAAFSCHW DGCTKYRTPT KQRLAQ LMA HVKTHLRAEE AQQHKMSQLT GPNDPNQPLT ASPTVSKRHP PHQPHASRQG RVIRPATTIT ITYEETASAR DERNPNA PP QAAGIPLSAV LILRNIARNV PHTNLEREER ERAAEAAGQG EQQAQVVVSN DREKEIDDDA GSRIKAIWNE RLFRPVLP R LWEVFTENRL LAPYVASLFS LLERDDRDYS SEGYFFHHHH HH

UniProtKB: Chromatin structure-remodeling complex protein

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Macromolecule #6: Putative chromatin structure-remodeling complex protein

MacromoleculeName: Putative chromatin structure-remodeling complex protein
type: protein_or_peptide / ID: 6 / Details: The C-terminal 8aa encode Flag tag / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila DSM 1495 (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 66.694156 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAVRSDSQQA VFSSYAPRLR TYNNALLQPI LPGSGQGPLS RTTKRGTAII NYAEDGYDDL DDDEDDGRRR PTGLRSLRRD DSTSKVDPS EKVGKDTSVP VEMQGYWRDW MGKNKPNRSD VHNYVQACLP LTLIPIRIDL DIPSFTPQPP LPPVPGGSVD N THPLYKPQ ...String:
MAVRSDSQQA VFSSYAPRLR TYNNALLQPI LPGSGQGPLS RTTKRGTAII NYAEDGYDDL DDDEDDGRRR PTGLRSLRRD DSTSKVDPS EKVGKDTSVP VEMQGYWRDW MGKNKPNRSD VHNYVQACLP LTLIPIRIDL DIPSFTPQPP LPPVPGGSVD N THPLYKPQ EPTVPYRLRD TFLWNLHESL ITTDYFATQL VQDLDLPNKQ QTIAEISKQI RTQLEEYAGV ALHPLFHART NR AKATADN TTKGTTPAPN QAQGASRADT PNAPITSFKP GAAGSSVVQT AQGEVSARAT PIPPEAGDDS PDDTYRCIIN LNI TLSSQV YTDKFEWSLL HPPGTAEIFA KQTCADLGLY GEWVPALTHA IYEAVLKLKK EACEAGGLVA GWTAFGSGTL AGGA GTEFP NDAAPTAGGE GAGWRYDPEH LAEEWEPKLE TLSKEEIEKR EGDRERQIRR LRRETARFSS NTGMAGGIPI GFAFG SLIE QEQEERLAKL RRRRRFRSPS PGRDSVAPGR GTPAAGGAGG GGAEGYGGGG GTLTEAERTT WRCSHCRVWG TSVWAV RDG PFGPRSLCNN CGFMYERDRK LPRWTKMLHA NDPRPFDYKD DDDK

UniProtKB: Chromatin structure-remodeling complex protein

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Macromolecule #7: Rsc7

MacromoleculeName: Rsc7 / type: protein_or_peptide / ID: 7 / Details: The C-terminal 8aa encode Flag tag / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila DSM 1495 (fungus)
Molecular weightTheoretical: 60.565992 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MPSRRSGRAA AKRAQQALES TPKTFEGIDD EDEPMPDAAE QEDAEAKDDA DEDGDKDDGS VVEEEAVEED EEESAKSPTP PPEPVVRRR RLGRPPKNRP PDWDQLPIEP PNPDATPRRR GRGGWRGRGG RKGQQQQPQQ QAVEVDGVFY NIVDDELDLP E DPEGEEKV ...String:
MPSRRSGRAA AKRAQQALES TPKTFEGIDD EDEPMPDAAE QEDAEAKDDA DEDGDKDDGS VVEEEAVEED EEESAKSPTP PPEPVVRRR RLGRPPKNRP PDWDQLPIEP PNPDATPRRR GRGGWRGRGG RKGQQQQPQQ QAVEVDGVFY NIVDDELDLP E DPEGEEKV DKLGNLKGGR EYRCRTFTVL GRGERLYMLS TEPARCVGFR DSYLFFTKHK RLHKIIVNDE EKRDMIERDI IP HSYKGRS IGIVTARSVF REFGAQIIVG GRRIIDDYEV AKARADGVVE GELADPNDIF DPDKPYNKNQ YVAWHGASSV YHS GGPSVP QQNVKADTKK RRVAVNDVNW QLEHAREASH FNSVLSAVRR QNLNGVYDIH TNTMFYPRTM QPTHARIVQV NDSE VSPDA HKSKVFPPLK PSISRNFLVL DTHLETPPAG ISPAAYDVPF RTSRADYENW LATDFTAPFK GLMAVSDEIR DLLPP ECRA AFDRAREQEE NWFAKWGDEK DCTSRREPII DKAIVPYSMT LGDYKDDDDK

UniProtKB: Uncharacterized protein

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Macromolecule #8: Rsc58

MacromoleculeName: Rsc58 / type: protein_or_peptide / ID: 8 / Details: The C-terminal 6aa encode 6xHis tag / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila DSM 1495 (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 107.232539 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MASPDVAPPQ ANGVAALSRP DSPASSIASQ STKRKREPAD EAAVDPSLSA ISDSQKPTLN GQRAASECMP LIRDFFAVLE SFDSSESPI LKRHLPEPVS HGEPSTKRAK SEDPSKPLTI ADKVTQNAYE NLDDFVVDVA RATDARIKDL EAAGATADEN A IAEAAAFK ...String:
MASPDVAPPQ ANGVAALSRP DSPASSIASQ STKRKREPAD EAAVDPSLSA ISDSQKPTLN GQRAASECMP LIRDFFAVLE SFDSSESPI LKRHLPEPVS HGEPSTKRAK SEDPSKPLTI ADKVTQNAYE NLDDFVVDVA RATDARIKDL EAAGATADEN A IAEAAAFK TKALELYRRE LAYPNVPPPS SSGKVSSIST RTGGVVLSTV GMAPTMRPLF TSLRQAPDGS NKPLPETALP NG VSVTRVL QEPPLTGDKS GRTPTLGELF PSPRNLPPLQ PPKAPKTTTK SNVLTFYHPE FIEKSKYRTG TYFSQVLSQG VWL DYTNAT PTVHTKTKQR ERAQSLAGVK PSSIELEMTE LEALFRGAFS SFAPSRDDSG AIVPAQQVSR IWYQRIGRRN LNKM VEEIE PEVAEEDGVP ARADSAVIME VDEERVKEVI ENWDDSLIDP NLDSVLGKKS DEEKQVDDIL EEISDMIETL ASYQR NRNL TLPTSQDRYS ADPVNGDMLR NGSASHQPSE EEMMAYQALK SQLALIIQTL PPWAVARLNS DKLAELAVST KIEVRS DDY RGVMEEDEPA RQARLAAQVA TTNQRQSQRA PSVAAAQYAN HQYQPQFTHS ARPVPNAPHY QPQTPVRPQP NVYSRAP SA VPIPQPHHQA RPQPPPAQYR PQMYAPQLAK AQGPYGHSGM PQQYVPSPAQ QPRMPPQQPP PPHYNNMVPA GAPPNSRF P QAYPAGYPQQ QPIHPQHMPP HAQSVPMPPQ HHPPMTQQPM PAPYSPYPNG APMPRTMSPQ VPPQPPHTYA QSPTPQHQQ MPRPPPPYGP GTTQPGMPPN TIPRPPPQQY VPGSPGPAGM MPARPPQHQP VAPTPPPGQH PLPAAAMPSY APVMTTTADN LQPRPATPG HQAPPPGGVV GLAGIGLAGQ MDVHKLAAAK MQMAQAQQRT QTPVPVPQVP GQVPPPQLVN GTGVRTVAPS P SPVPGTPT VAPAPVPVPP QQQPSAHHHH HH

UniProtKB: Uncharacterized protein

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Macromolecule #9: Rsc1

MacromoleculeName: Rsc1 / type: protein_or_peptide / ID: 9 / Details: The C-terminal 8aa encode Flag tag / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila DSM 1495 (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 112.634406 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MPTLRERPSR GEASPAKSAS TAATRSTPRT RRTSMPGDAI EVRESIEAKG DVDEDIEMED ADGSATPRKN KDKNKDKDAD GDIKMGGAS GTGDVDADGE PDDEVAQTQQ DDSQRDLLQL IRETSEYLCR YTIKIDGEEH EIASGFQRLV SKRSLPDYFE V IKEPMAFS ...String:
MPTLRERPSR GEASPAKSAS TAATRSTPRT RRTSMPGDAI EVRESIEAKG DVDEDIEMED ADGSATPRKN KDKNKDKDAD GDIKMGGAS GTGDVDADGE PDDEVAQTQQ DDSQRDLLQL IRETSEYLCR YTIKIDGEEH EIASGFQRLV SKRSLPDYFE V IKEPMAFS TIRAKIGKKT YQKFSEFVHD VTRICHNAQV YNRPSAPIFA DAAKLLEVFK EKLDELVKEG AITAEEAQIP DL GPLPEFE ESPPPEEEEE EEEEEEEEEE EEEDDDSDDD GRRRSRRRNR KGASRRGGDD DDDDDYKKQR PGRPPKVLTP MEA RIESLL KGLRKFKNEN GQPRVVYFDR LPEKGEPISL DIIKKNHKRK KYQNVDEVLQ DLLRMFNNAK QHHDENSQVY KDAV ELEKQ ARLLAEIEKA RPDEDFRGED GKLPLASIEH KGEIWKVGDW VHIRNPNDLS KPIVAQIFRT WSDKSGQKWV NACWY YRPE QTVHRFDKHF YENEVVKTGQ YRDHRIDDIE DRCFVMFITR YPRGRPRGLP PDKAVYVCES RYNEETFKFN KIKTWS GCL PDEVREKDYE MDLFDVPRVM RKFPSPIKHL LQADAKETDP LPKPTWRSPN APPLIGAVHR RPREPNESPP PEPHPVP VP VAPGAVPLPM APQVPIKANV PGVTGPPPPS YHHPAVLTPA PAPSPAHYQV QHFAPRQAPP PVPQQVPGPP MPHFQPQP Q PMPMHVPPQP GMMPHPMQQM PPAPHFPPAP HAYPPQYGAQ PPQAPPPPNY YQPAPNHIAS VPYEQHHRPV HAAPPPVTP SRPPPPMAPA PPNITTMPPH ASPHVSAPPP APAPHVYNAP RAPEVYTLAD NIDAAIPKEV REQFQCDENG RILFFTGAPL DRPANRVAE AYSQLGHSVR HLASIKQLRE ERARKRKERD EALARERAAQ EEEAKRQRLA QEQDSNDAAT LEQKKAEARE L LEKVLRDW AAEIDRGTQL LREDLDYKDD DDK

UniProtKB: Uncharacterized protein

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Macromolecule #10: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 10 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration2 mg/mL
BufferpH: 8
Component:
ConcentrationFormulaName
30.0 mMKClPotassium chloride
2.0 mMMgCl2Magnesium chloride
20.0 mMC8H18N2O4SHEPEs
0.5 mMC10H15N5O10P2ADP
1.0 mMBeSO4Beryllium sulfate
8.0 mMNaFsodium fluoride
GridModel: Quantifoil R1.2/1.3
VitrificationCryogen name: ETHANE
DetailsThis sample was homogenous and monodisperse.

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.4 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.12 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 250420
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: MAXIMUM LIKELIHOOD

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-23cp:
Cryo-EM structure of RSC complex from Chaetomium thermophilum

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