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- EMDB-68782: In situ cryo sub-tomogram average of the ciliary axoneme from Rgs... -

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Entry
Database: EMDB / ID: EMD-68782
TitleIn situ cryo sub-tomogram average of the ciliary axoneme from Rgs22 knockout mouse ependymal progenitor cells
Map data
Sample
  • Organelle or cellular component: In situ cryo sub-tomogram average of the ciliary axoneme from Rgs22 knockout mouse ependymal progenitor cells
    • Organelle or cellular component: Ciliary axoneme of Microtubule doublets,Outer dynein arms,Inner dynein arms and Radial spokes
KeywordsAxoneme / Motile cilia / Ependymal cell / In situ / STRUCTURAL PROTEIN
Biological speciesMus musculus (house mouse)
Methodsubtomogram averaging / cryo EM / Resolution: 34.0 Å
AuthorsYe-Jun P
Funding support1 items
OrganizationGrant numberCountry
Other government
CitationJournal: Nat Commun / Year: 2026
Title: RGS22 is a metazoa-specific radial spoke component required for coordinated ciliary beating.
Authors: Anxuan Fang / Jiajun Luo / Haomang Xu / Ye-Jun Peng / Wei Xu / Huiwen Cao / Wei Li / Jialei Dai / Zhipeng Shi / Yong Wang / Xiao-Meng Li / Huijie Zhao / Miao Gui / Chao Yu / Xueliang Zhu / Qianting Zhang /
Abstract: In metazoan epithelia, arrays of motile cilia beat in a tissue-wide coordinated manner to drive unidirectional fluid flow. Whether radial spokes (RSs), which mediate mechano-signal transduction ...In metazoan epithelia, arrays of motile cilia beat in a tissue-wide coordinated manner to drive unidirectional fluid flow. Whether radial spokes (RSs), which mediate mechano-signal transduction between the central pair and axonemal dynein arms, acquire metazoa-specific subunit(s) in evolution to facilitate this function remains poorly known. Here we report that Gα-GTPase activating protein family member RGS22 is a metazoa-specific component of RS1/2 complexes. Mouse Rgs22-deficient motile cilia exhibit ultrastructural defects, including deformation of the RS1 and disorganization of outer dynein arms. These abnormalities correlate with impaired planar polarity, manifested as unsynchronized ciliary beating and disrupted rotational polarity of basal bodies across the ependyma. The motility deficits further lead to progressive ciliary loss and shortening, resulting in postnatal hydrocephalus. Together, our data identify RGS22 an animal-lineage RS1/2 component required for normal RS architecture and associated with tissue-level ciliary motility and coordination phenotypes.
History
DepositionJan 28, 2026-
Header (metadata) releaseJul 22, 2026-
Map releaseJul 22, 2026-
UpdateJul 22, 2026-
Current statusJul 22, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_68782.png

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Map

FileReleased
Projections & slices

Image control

Size
Brightness
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AxesZ (Sec.)Y (Row.)X (Col.)
6.02 Å/pix.
x 240 pix.
= 1445.125 Å		6.02 Å/pix.
x 240 pix.
= 1445.125 Å		6.02 Å/pix.
x 240 pix.
= 1445.125 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 6.02136 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.001
Minimum - Maximum-0.0033494327 - 0.0071595553
Average (Standard dev.)0.000044112643 (±0.00075131084)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions240240240
Spacing240240240
CellA=B=C: 1445.1255 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_68782_half_map_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_68782_half_map_2.map
Projections & Slices
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Sample components

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Entire : In situ cryo sub-tomogram average of the ciliary axoneme from Rgs...

EntireName: In situ cryo sub-tomogram average of the ciliary axoneme from Rgs22 knockout mouse ependymal progenitor cells
Components
  • Organelle or cellular component: In situ cryo sub-tomogram average of the ciliary axoneme from Rgs22 knockout mouse ependymal progenitor cells
    • Organelle or cellular component: Ciliary axoneme of Microtubule doublets,Outer dynein arms,Inner dynein arms and Radial spokes

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Supramolecule #1: In situ cryo sub-tomogram average of the ciliary axoneme from Rgs...

SupramoleculeName: In situ cryo sub-tomogram average of the ciliary axoneme from Rgs22 knockout mouse ependymal progenitor cells
type: organelle_or_cellular_component / ID: 1 / Parent: 0
Source (natural)Organism: Mus musculus (house mouse)

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Supramolecule #2: Ciliary axoneme of Microtubule doublets,Outer dynein arms,Inner d...

SupramoleculeName: Ciliary axoneme of Microtubule doublets,Outer dynein arms,Inner dynein arms and Radial spokes
type: organelle_or_cellular_component / ID: 2 / Parent: 1
Source (natural)Organism: Mus musculus (house mouse)

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statefilament

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Sample preparation

BufferpH: 7.8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Average electron dose: 2.5 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 34.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number subtomograms used: 749
ExtractionNumber tomograms: 124 / Number images used: 37589 / Software - Name: RELION
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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