ジャーナル: Mol Biol Cell / 年: 2018 タイトル: A microtubule-dynein tethering complex regulates the axonemal inner dynein f (I1). 著者: Tomohiro Kubo / Yuqing Hou / Deborah A Cochran / George B Witman / Toshiyuki Oda / 要旨: Motility of cilia/flagella is generated by a coordinated activity of thousands of dyneins. Inner dynein arms (IDAs) are particularly important for the formation of ciliary/flagellar waveforms, but ...Motility of cilia/flagella is generated by a coordinated activity of thousands of dyneins. Inner dynein arms (IDAs) are particularly important for the formation of ciliary/flagellar waveforms, but the molecular mechanism of IDA regulation is poorly understood. Here we show using cryoelectron tomography and biochemical analyses of Chlamydomonas flagella that a conserved protein FAP44 forms a complex that tethers IDA f (I1 dynein) head domains to the A-tubule of the axonemal outer doublet microtubule. In wild-type flagella, IDA f showed little nucleotide-dependent movement except for a tilt in the f β head perpendicular to the microtubule-sliding direction. In the absence of the tether complex, however, addition of ATP and vanadate caused a large conformational change in the IDA f head domains, suggesting that the movement of IDA f is mechanically restricted by the tether complex. Motility defects in flagella missing the tether demonstrates the importance of the IDA f-tether interaction in the regulation of ciliary/flagellar beating.
ダウンロード / ファイル: emd_6871.map.gz / 形式: CCP4 / 大きさ: 52.7 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Chlamydomonas fap44 mutant, IDA f in ATP plus vanadate state (state III)
ボクセルのサイズ
X=Y=Z: 7.025 Å
密度
表面レベル
登録者による: 376. / ムービー #1: 376
最小 - 最大
0. - 629.348139999999944
平均 (標準偏差)
3.0684407 (±30.542545)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
240
240
240
Spacing
240
240
240
セル
A=B=C: 1686.0 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
7.025
7.025
7.025
M x/y/z
240
240
240
origin x/y/z
0.000
0.000
0.000
length x/y/z
1686.000
1686.000
1686.000
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
240
240
240
D min/max/mean
0.000
629.348
3.068
-
添付データ
-
試料の構成要素
-
全体 : Inner dynein f
全体
名称: Inner dynein f
要素
細胞器官・細胞要素: Inner dynein f
-
超分子 #1: Inner dynein f
超分子
名称: Inner dynein f / タイプ: organelle_or_cellular_component / ID: 1 / 親要素: 0 詳細: fap44 mutant Chlamydomonas axoneme in ATP plus vanadate state (state III)