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- EMDB-68593: Structure of ozureprubart Fab in complex with human IgE-Fc -

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Basic information

Entry
Database: EMDB / ID: EMD-68593
TitleStructure of ozureprubart Fab in complex with human IgE-Fc
Map data
Sample
  • Complex: ozureprubart Fab in complex with human IgE-Fc
    • Protein or peptide: ozureprubart Fab heavy chain
    • Protein or peptide: ozureprubart Fab light chain
    • Protein or peptide: IgE-Fc
KeywordsOzureprubart IgE antiallergic antibody / IMMUNE SYSTEM
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.96 Å
AuthorsZhou F / Liu PP
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: Ozureprubart: Mechanistic and Functional Insights into a Long-Acting Anti-IgE Therapy
Authors: Zhou F / Liu PP
History
DepositionJan 20, 2026-
Header (metadata) releaseFeb 4, 2026-
Map releaseFeb 4, 2026-
UpdateFeb 4, 2026-
Current statusFeb 4, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_68593.png

Downloads & links

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Map

FileDownload / File: emd_68593.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.82 Å/pix.
x 360 pix.
= 294.84 Å		0.82 Å/pix.
x 360 pix.
= 294.84 Å		0.82 Å/pix.
x 360 pix.
= 294.84 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.819 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.113
Minimum - Maximum-0.6307001 - 0.91551536
Average (Standard dev.)0.00008012615 (±0.014020521)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 294.84 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_68593_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_68593_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_68593_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : ozureprubart Fab in complex with human IgE-Fc

EntireName: ozureprubart Fab in complex with human IgE-Fc
Components
  • Complex: ozureprubart Fab in complex with human IgE-Fc
    • Protein or peptide: ozureprubart Fab heavy chain
    • Protein or peptide: ozureprubart Fab light chain
    • Protein or peptide: IgE-Fc

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Supramolecule #1: ozureprubart Fab in complex with human IgE-Fc

SupramoleculeName: ozureprubart Fab in complex with human IgE-Fc / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: ozureprubart Fab heavy chain

MacromoleculeName: ozureprubart Fab heavy chain / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.836615 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: EVQLVESGGG LVQPGGSLRL SCAVSGYSIT SGYSWNWVRQ APGKGLEWVS VITYAGSTNY ADSVKGRFTI SRDDSKNTFY LQMNSLRAE DTAVYYCARG THYFGHWHFA VWGQGTLVTV SSASTKGPSV FPLAPSSKST SGGTAALGCL VKDYFPEPVT V SWNSGALT ...String:
EVQLVESGGG LVQPGGSLRL SCAVSGYSIT SGYSWNWVRQ APGKGLEWVS VITYAGSTNY ADSVKGRFTI SRDDSKNTFY LQMNSLRAE DTAVYYCARG THYFGHWHFA VWGQGTLVTV SSASTKGPSV FPLAPSSKST SGGTAALGCL VKDYFPEPVT V SWNSGALT SGVHTFPAVL QSSGLYSLSS VVTVPSSSLG TQTYICNVNH KPSNTKVDKK VEPKSC

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Macromolecule #2: ozureprubart Fab light chain

MacromoleculeName: ozureprubart Fab light chain / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.857256 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: DIQLTQSPSS LSASVGDRVT ITCRASQSVG DEADSYMNWY QQKPGKAPKL LIYAASYLQS GVPSRFSGSG SGTDFTLTIS SLQPEDFAT YYCQQSHEDP YTFGQGTKLE IKRTVAAPSV FIFPPSDEQL KSGTASVVCL LNNFYPREAK VQWKVDNALQ S GNSQESVT ...String:
DIQLTQSPSS LSASVGDRVT ITCRASQSVG DEADSYMNWY QQKPGKAPKL LIYAASYLQS GVPSRFSGSG SGTDFTLTIS SLQPEDFAT YYCQQSHEDP YTFGQGTKLE IKRTVAAPSV FIFPPSDEQL KSGTASVVCL LNNFYPREAK VQWKVDNALQ S GNSQESVT EQDSKDSTYS LSSTLTLSKA DYEKHKVYAC EVTHQGLSSP VTKSFNRGEC

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Macromolecule #3: IgE-Fc

MacromoleculeName: IgE-Fc / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.092285 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: NPRCVSAYLS RPSPFDLFIR KSPTITCLVV DLAPSKGTVN LTWSRASGKP VNHSTRKEEK QRNGTLTVTS TLPVGTRDWI EGETYQCRV THPHLPRALM RSTTKTSGPR AAPEVYAFAT PEWPGSRDKR TLACLIQNFM PEDISVQWLH NEVQLPDARH S TTQPRKTK ...String:
NPRCVSAYLS RPSPFDLFIR KSPTITCLVV DLAPSKGTVN LTWSRASGKP VNHSTRKEEK QRNGTLTVTS TLPVGTRDWI EGETYQCRV THPHLPRALM RSTTKTSGPR AAPEVYAFAT PEWPGSRDKR TLACLIQNFM PEDISVQWLH NEVQLPDARH S TTQPRKTK GSGFFVFSRL EVTRAEWEQK DEFICRAVHE AASPSQTVQR AVSVNP

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 65.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.4 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER / Details: cryoSPARC Ab-initio reconstruction
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.96 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 85120
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION
FSC plot (resolution estimation)

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