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- EMDB-67259: The structure of the complex formed by two FIPV-II S proteins and... -

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Basic information

Entry
Database: EMDB / ID: EMD-67259
TitleThe structure of the complex formed by two FIPV-II S proteins and two cat APN proteins.
Map data
Sample
  • Complex: The structure of the complex formed by two FIPV-II S proteins and two cat APN proteins.
Keywordstwo FIPV-II S proteins and two cat APN / PROTEIN BINDING
Biological speciesFelis catus (domestic cat)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.53 Å
AuthorsSun JQ / Niu S
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: To Be Published
Title: The structure of the complex formed by two FIPV-II S proteins and two cat APN proteins.
Authors: Sun JQ / Niu S
History
DepositionNov 23, 2025-
Header (metadata) releaseJan 7, 2026-
Map releaseJan 7, 2026-
UpdateJan 7, 2026-
Current statusJan 7, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_67259.png

Downloads & links

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Map

FileDownload / File: emd_67259.map.gz / Format: CCP4 / Size: 2.7 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.81 Å/pix.
x 900 pix.
= 727.2 Å		0.81 Å/pix.
x 900 pix.
= 727.2 Å		0.81 Å/pix.
x 900 pix.
= 727.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.808 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0689
Minimum - Maximum-0.31272516 - 0.93753624
Average (Standard dev.)0.000011885511 (±0.016730886)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions900900900
Spacing900900900
CellA=B=C: 727.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_67259_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_67259_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : The structure of the complex formed by two FIPV-II S proteins and...

EntireName: The structure of the complex formed by two FIPV-II S proteins and two cat APN proteins.
Components
  • Complex: The structure of the complex formed by two FIPV-II S proteins and two cat APN proteins.

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Supramolecule #1: The structure of the complex formed by two FIPV-II S proteins and...

SupramoleculeName: The structure of the complex formed by two FIPV-II S proteins and two cat APN proteins.
type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Felis catus (domestic cat)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: OTHER

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.53 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 71481
Initial angle assignmentType: OTHER
Final angle assignmentType: OTHER

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