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- EMDB-67148: Local refinement map of the cytoplasmic lattice (CPL) from mouse ... -

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Basic information

Entry
Database: EMDB / ID: EMD-67148
TitleLocal refinement map of the cytoplasmic lattice (CPL) from mouse oocyte at 4.15 angstrom
Map data
Sample
  • Complex: Mouse oocyte cytoplasmic lattices (CPL)
KeywordsCytoplasmic lattice / Maternal complex / Cryo-EM structure / Protein assembly / STRUCTURAL PROTEIN / CYTOSOLIC PROTEIN
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.1 Å
AuthorsLiu SX / Liu YS / Gao HS / Shen EZ
Funding support China, 2 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32070628 China
National Natural Science Foundation of China (NSFC)32271258 China
CitationJournal: To Be Published
Title: Cryo-EM structure of the Cytoplasmic lattice(CPL) from mouse oocyte
Authors: Liu SX / Gao HS / Shen EZ
History
DepositionNov 19, 2025-
Header (metadata) releaseMar 4, 2026-
Map releaseMar 4, 2026-
UpdateMar 4, 2026-
Current statusMar 4, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_67148.png

Downloads & links

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Map

FileDownload / File: emd_67148.map.gz / Format: CCP4 / Size: 1000 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.08 Å/pix.
x 640 pix.
= 689.472 Å		1.08 Å/pix.
x 640 pix.
= 689.472 Å		1.08 Å/pix.
x 640 pix.
= 689.472 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.0773 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.2
Minimum - Maximum-0.5116174 - 0.91822416
Average (Standard dev.)-0.0010530418 (±0.023408849)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions640640640
Spacing640640640
CellA=B=C: 689.472 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_67148_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_67148_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Mouse oocyte cytoplasmic lattices (CPL)

EntireName: Mouse oocyte cytoplasmic lattices (CPL)
Components
  • Complex: Mouse oocyte cytoplasmic lattices (CPL)

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Supramolecule #1: Mouse oocyte cytoplasmic lattices (CPL)

SupramoleculeName: Mouse oocyte cytoplasmic lattices (CPL) / type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 2 MDa

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: EMDB MAP
EMDB ID:

65575

Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 676923
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION

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