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- EMDB-66502: apo state of Mengla Virus Glycoprotein -

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Basic information

Entry
Database: EMDB / ID: EMD-66502
Titleapo state of Mengla Virus Glycoprotein
Map data
Sample
  • Complex: glycoprotein of mengla virus
    • Protein or peptide: Envelope glycoprotein
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
KeywordsMengla Virus Glycoprotein / VIRAL PROTEIN
Function / homologyTLV/ENV coat polyprotein / : / Filoviruses glycoprotein, extracellular domain / Filovirus glycoprotein / Envelope glycoprotein GP2-like, HR1-HR2 / viral envelope / host cell plasma membrane / Envelope glycoprotein
Function and homology information
Biological speciesDianlovirus menglaense
Methodsingle particle reconstruction / cryo EM / Resolution: 3.08 Å
AuthorsWang L / Zou B / Liu B / Xue L / He J / Xiong X / Xiaoli X
Funding support China, 2 items
OrganizationGrant numberCountry
Other governmentSRPG22-002 China
Other governmentGIBHBRP24-02 China
CitationJournal: To Be Published
Title: Cryo-EM Structures Reveal Mengla Virus GP uses combined Ebola- and Marburg-Like Epitope Masking Strategies to Evade Antibodies
Authors: Wang LY / Zou BQ / Liu BH / Ma Y / Xue L / Habib G / Yang XL / Chen XW / Zhang Y / Yang ZF / He J / Xiong XL
History
DepositionOct 9, 2025-
Header (metadata) releaseJun 3, 2026-
Map releaseJun 3, 2026-
UpdateJun 3, 2026-
Current statusJun 3, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_66502.png

Downloads & links

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Map

FileDownload / File: emd_66502.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.58 Å/pix.
x 480 pix.
= 277.44 Å		0.58 Å/pix.
x 480 pix.
= 277.44 Å		0.58 Å/pix.
x 480 pix.
= 277.44 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.578 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.216
Minimum - Maximum-0.0021937308 - 2.1349304
Average (Standard dev.)0.001447859 (±0.028633986)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 277.44 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_66502_msk_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_66502_half_map_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_66502_half_map_2.map
Projections & Slices
AxesZYX

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Slices (1/2)
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Histogram

Histogram (log scale)

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Sample components

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Entire : glycoprotein of mengla virus

EntireName: glycoprotein of mengla virus
Components
  • Complex: glycoprotein of mengla virus
    • Protein or peptide: Envelope glycoprotein
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose

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Supramolecule #1: glycoprotein of mengla virus

SupramoleculeName: glycoprotein of mengla virus / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Dianlovirus menglaense

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Macromolecule #1: Envelope glycoprotein

MacromoleculeName: Envelope glycoprotein / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Dianlovirus menglaense
Molecular weightTheoretical: 73.748773 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MALAKKYLMR IFIISHYFLS YNYGFFLPIV KLDIQPENEI IKSPCNGVVK KTEDIHLMSL TLTGQKTASD PMAASKRWAF KEGVLPKTV AFTEGEEAKD CFNISIVDLQ GRSLLLEAPS GIKDYPRCKS IHHIQGQNPH AKGNVLHLWG AFFLYDRIAS T TMFKDKVF ...String:
MALAKKYLMR IFIISHYFLS YNYGFFLPIV KLDIQPENEI IKSPCNGVVK KTEDIHLMSL TLTGQKTASD PMAASKRWAF KEGVLPKTV AFTEGEEAKD CFNISIVDLQ GRSLLLEAPS GIKDYPRCKS IHHIQGQNPH AKGNVLHLWG AFFLYDRIAS T TMFKDKVF AEGNVAALVI NKTVHHQIFK INKIPQNETI GSCNHTYWRS DAGDGFNTTL AVTNLAYVLS ANESTKIFKQ MQ DDLRKRI ENMTSSNLTT LSPEAISNSS DANSSATIAY GTSGASISAT TRSATSTLTI TTDGQMSPPP TPAPPSPSLK PST DDIKTP TVLSTTSSST RSKRSVTVAK QSSANPTGKQ RGRGRKGPST GTLTEEQIER VRMLIQKNVG RVNYNNLPLI AGLT ALKKK RQVLETFSGE IELEELIGDE KLDSRFRENL KMILETLRNE SYSHFELDIP KTAALKSPEE TDPRIHMEYL GSGEE PIQS NGQTDTDCDP SLRIWTPNEE KLAAGVSWIP YFGPGAEGLY TMGLMQNQND VVCRLRRLAN QTAKSLDLLL RVTTEE RTY SIINRHAIDF LLARWGGTCR VLGPECCIGV EDLTKNITDQ IQQMRRDEQD EYEGWGKGGN WFNSQFGNWL NLGILIF LF LGIIIALSCV CRLFMNLTVY

UniProtKB: Envelope glycoprotein

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Macromolecule #4: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 4 / Number of copies: 3 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.4 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionApplied symmetry - Point group: C3 (3 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 3.08 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 19463
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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