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- EMDB-66218: Cryo-electron tomogram of vesicular stomatitis virus (VSV) with r... -

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Basic information

Entry
Database: EMDB / ID: EMD-66218
TitleCryo-electron tomogram of vesicular stomatitis virus (VSV) with rabies virus glycoprotein
Map data
Sample
  • Virus: Vesicular stomatitis virus
    • Complex: Vesicular stomatitis virus with rabies virus glycoprotein
KeywordsCryo-electron tomography / vesicular stomatitis virus / rabies virus glycoprotein / VIRUS
Biological speciesHuman papillomavirus / Vesicular stomatitis virus
Methodelectron tomography / cryo EM
AuthorsLiu L / Zheng Q / Li S / Xia N
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: iScience / Year: 2025
Title: An engineered VSV-vectored rabies vaccine with an RABV-G-L71S mutation.
Authors: Mingxi Yue / Yuhe Zhu / Hongchao Wu / Siping Yan / Jinlei Zhang / Mengqi Chang / Liqin Liu / Hui Huang / Jie Lu / Wenrong Wu / Ruitong Chen / Shuyu Wu / Yibing Gao / Yujiao Cao / Hangtian ...Authors: Mingxi Yue / Yuhe Zhu / Hongchao Wu / Siping Yan / Jinlei Zhang / Mengqi Chang / Liqin Liu / Hui Huang / Jie Lu / Wenrong Wu / Ruitong Chen / Shuyu Wu / Yibing Gao / Yujiao Cao / Hangtian Ding / Qingbing Zheng / Yingbin Wang / Quan Yuan / Hualong Xiong / Kegong Tian / Tianying Zhang / Ningshao Xia /
Abstract: A replicating recombinant rabies vaccine, rVSV-RABV, was constructed using vesicular stomatitis virus (VSV) expressing the rabies virus glycoprotein (RABV-G, SAD-B19 strain). A leucine-to-serine ...A replicating recombinant rabies vaccine, rVSV-RABV, was constructed using vesicular stomatitis virus (VSV) expressing the rabies virus glycoprotein (RABV-G, SAD-B19 strain). A leucine-to-serine mutation at position 71 (L71S) in the RABV-G extracellular domain significantly enhanced the viral titer and G protein expression. The derived strain, rVSV-RABV-L71S, demonstrated production and immunogenic advantages. As an inactivated vaccine formulated with GEL-02 adjuvant, it induced stronger neutralizing antibody responses, along with innate and Th1-biased T cell immunity in mice, compared to commercially inactivated vaccines. As a live vaccine, it conferred robust protection against the CVS-24 challenge in mice (NIH titer ∼4.8-fold higher than reference) and elicited superior neutralizing antibodies in dogs. Both inactivated and live rVSV-RABV-L71S formulations exhibited favorable safety profiles. rVSV-RABV-L71S represents a promising, low-cost, high-efficacy rabies vaccine candidate suitable for widespread use in developing countries.
History
DepositionSep 16, 2025-
Header (metadata) releaseJul 29, 2026-
Map releaseJul 29, 2026-
UpdateJul 29, 2026-
Current statusJul 29, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_66218.png

Downloads & links

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Map

FileDownload / File: emd_66218.map.gz / Format: CCP4 / Size: 210.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
11.04 Å/pix.
x 720 pix.
= 7948.8 Å		11.04 Å/pix.
x 150 pix.
= 1656. Å		11.04 Å/pix.
x 510 pix.
= 5630.4 Å

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 11.04 Å
Density

Histogram

Histogram (log scale)
Minimum - Maximum0.0 - 528196288.0
Average (Standard dev.)152654832.0 (±13639189.0)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions150510720
Spacing510150720
CellA: 5630.4 Å / B: 1656.0 Å / C: 7948.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Vesicular stomatitis virus

EntireName: Vesicular stomatitis virus
Components
  • Virus: Vesicular stomatitis virus
    • Complex: Vesicular stomatitis virus with rabies virus glycoprotein

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Supramolecule #1: Vesicular stomatitis virus

SupramoleculeName: Vesicular stomatitis virus / type: virus / ID: 1 / Parent: 0 / Macromolecule list: #1-#7 / NCBI-ID: 11276 / Sci species name: Vesicular stomatitis virus / Virus type: VIRUS-LIKE PARTICLE / Virus isolate: OTHER / Virus enveloped: No / Virus empty: Yes

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Supramolecule #2: Vesicular stomatitis virus with rabies virus glycoprotein

SupramoleculeName: Vesicular stomatitis virus with rabies virus glycoprotein
type: complex / ID: 2 / Parent: 1
Source (natural)Organism: Human papillomavirus

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Experimental details

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Structure determination

Methodcryo EM
Processingelectron tomography
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
GridModel: Quantifoil R2/2 / Material: GOLD / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV
SectioningOther: NO SECTIONING

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 123.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 5.0 µm / Nominal defocus min: 2.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionAlgorithm: BACK PROJECTION / Software - Name: AreTomo / Number images used: 41
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION

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