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- EMDB-66197: Cryo-EM structure of Escherichia coli FtsZ Filaments -

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Basic information

Entry
Database: EMDB / ID: EMD-66197
TitleCryo-EM structure of Escherichia coli FtsZ Filaments
Map data
Sample
  • Complex: FtsZ Filaments
    • Protein or peptide: Cell division protein FtsZ
  • Ligand: GUANOSINE-5'-DIPHOSPHATE
KeywordsBacteria Cell Division / CELL CYCLE
Function / homology
Function and homology information


divisome complex / division septum assembly / FtsZ-dependent cytokinesis / cell division site / protein polymerization / cell division / GTPase activity / GTP binding / identical protein binding / plasma membrane / cytoplasm
Similarity search - Function
FtsZ protein signature 2. / Cell division protein FtsZ / Cell division protein FtsZ, conserved site / Cell division protein FtsZ, C-terminal / FtsZ family, C-terminal domain / FtsZ protein signature 1. / Tubulin-like protein FtsZ/CetZ / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal ...FtsZ protein signature 2. / Cell division protein FtsZ / Cell division protein FtsZ, conserved site / Cell division protein FtsZ, C-terminal / FtsZ family, C-terminal domain / FtsZ protein signature 1. / Tubulin-like protein FtsZ/CetZ / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily
Similarity search - Domain/homology
Cell division protein FtsZ
Similarity search - Component
Biological speciesEscherichia coli K-12 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.0 Å
AuthorsLi J / Luo M
Funding support Singapore, China, 4 items
OrganizationGrant numberCountry
Ministry of Education (MoE, Singapore)A-0008412-00-00 Singapore
Ministry of Education (MoE, China)2024yc_yxhwbs10002 China
Ministry of Education (MoE, China)2024NSCQ-KJFZMSX0229 China
Ministry of Education (MoE, China)CSTB2024YCJH-KYXM0095 China
CitationJournal: To Be Published
Title: FtsZ Filaments
Authors: Li J / Xu X
History
DepositionSep 13, 2025-
Header (metadata) releaseSep 16, 2026-
Map releaseSep 16, 2026-
UpdateSep 16, 2026-
Current statusSep 16, 2026Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_66197.png

Downloads & links

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Map

FileDownload / File: emd_66197.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.06 Å/pix.
x 384 pix.
= 407.04 Å		1.06 Å/pix.
x 384 pix.
= 407.04 Å		1.06 Å/pix.
x 384 pix.
= 407.04 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.06 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.15
Minimum - Maximum-0.68792737 - 1.0172776
Average (Standard dev.)0.00024129417 (±0.019644666)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 407.03998 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_66197_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_66197_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : FtsZ Filaments

EntireName: FtsZ Filaments
Components
  • Complex: FtsZ Filaments
    • Protein or peptide: Cell division protein FtsZ
  • Ligand: GUANOSINE-5'-DIPHOSPHATE

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Supramolecule #1: FtsZ Filaments

SupramoleculeName: FtsZ Filaments / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Escherichia coli K-12 (bacteria)

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Macromolecule #1: Cell division protein FtsZ

MacromoleculeName: Cell division protein FtsZ / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli K-12 (bacteria)
Molecular weightTheoretical: 40.361758 KDa
Recombinant expressionOrganism: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria)
SequenceString: MFEPMELTND AVIKVIGVGG GGGNAVEHMV RERIEGVEFF AVNTDAQALR KTAVGQTIQI GSGITKGLGA GANPEVGRNA ADEDRDALR AALEGADMVF IAAGMGGGTG TGAAPVVAEV AKDLGILTVA VVTKPFNFEG KKRMAFAEQG ITELSKHVDS L ITIPNDKL ...String:
MFEPMELTND AVIKVIGVGG GGGNAVEHMV RERIEGVEFF AVNTDAQALR KTAVGQTIQI GSGITKGLGA GANPEVGRNA ADEDRDALR AALEGADMVF IAAGMGGGTG TGAAPVVAEV AKDLGILTVA VVTKPFNFEG KKRMAFAEQG ITELSKHVDS L ITIPNDKL LKVLGRGISL LDAFGAANDV LKGAVQGIAE LITRPGLMNV DFADVRTVMS EMGYAMMGSG VASGEDRAEE AA EMAISSP LLEDIDLSGA RGVLVNITAG FDLRLDEFET VGNTIRAFAS DNATVVIGTS LDPDMNDELR VTVVATGIGM DKR PEITLV TNKQVQQPVM DRYQQHGMAP LTQEQKPVAK VVNDNAPQTA KEPDYLDIPA FLRKQAD

UniProtKB: Cell division protein FtsZ

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Macromolecule #2: GUANOSINE-5'-DIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 2 / Number of copies: 4 / Formula: GDP
Molecular weightTheoretical: 443.201 Da
Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TECNAI 20
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Average exposure time: 6.02 sec. / Average electron dose: 40.2 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Calibrated defocus max: 2.5 µm / Calibrated defocus min: 1.0 µm / Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: Coot / Number images used: 117176
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE
FSC plot (resolution estimation)

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