EMDB-66105: Co-transcriptional histone H3K36 methylation complex containing R...

Basic information

Entry

Database: EMDB / ID: EMD-66105

• Title: Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp130, type A)

Sample

• Complex: Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp130, type A)
  • Protein or peptide: x 27 types
  • DNA: x 2 types
  • RNA: x 1 types
• Ligand: x 3 types

• Keywords: chromatin / nucleosome / TRANSCRIPTION

Function / homology

Function:

snoRNA transcription by RNA polymerase II / sno(s)RNA transcription / negative regulation of antisense RNA transcription / regulation of transcription initiation by RNA polymerase II / negative regulation of transcription elongation by RNA polymerase I / sno(s)RNA 3'-end processing / negative regulation of reciprocal meiotic recombination / positive regulation of transcription elongation by RNA polymerase I / mating-type region heterochromatin / histone H3K36 dimethyltransferase activity / transcription antitermination factor activity, DNA binding / regulation of septum digestion after cytokinesis / RNA polymerase II C-terminal domain phosphoserine binding / [histone H3]-lysine36 N-trimethyltransferase / regulation of transcription-coupled nucleotide-excision repair / ascospore formation / triplex DNA binding / Cdc73/Paf1 complex / co-transcriptional lncRNA 3' end processing, cleavage and polyadenylation pathway / histone H3K36 trimethyltransferase activity / siRNA-mediated pericentric heterochromatin formation / regulation of mRNA 3'-end processing / RNA polymerase I core binding / DSIF complex / regulation of rRNA processing / intracellular mRNA localization / RNA polymerase I general transcription initiation factor binding / rDNA binding / rDNA heterochromatin / negative regulation of DNA recombination / transcription elongation factor complex / intracellular phosphate ion homeostasis / negative regulation of mitophagy / transcription elongation-coupled chromatin remodeling / snRNP binding / mRNA 3'-end processing / U4 snRNA binding / transcription elongation factor activity / chromatin-protein adaptor activity / cellular response to stress / poly(A)+ mRNA export from nucleus / kinetochore assembly / nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / spliceosomal complex assembly / RNA polymerase II complex binding / termination of RNA polymerase II transcription / U5 snRNA binding / 7-methylguanosine mRNA capping / termination of RNA polymerase I transcription / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / transcription initiation at RNA polymerase I promoter / positive regulation of translational initiation / mitotic metaphase chromosome alignment / U2 snRNA binding / nuclear-transcribed mRNA catabolic process / U6 snRNA binding / negative regulation of tumor necrosis factor-mediated signaling pathway / translation elongation factor activity / U1 snRNA binding / pericentric heterochromatin / regulation of DNA-templated DNA replication initiation / RNA polymerase II core promoter sequence-specific DNA binding / termination of RNA polymerase III transcription / transcription initiation at RNA polymerase III promoter / RNA polymerase I complex / nucleosome binding / RNA polymerase III complex / negative regulation of megakaryocyte differentiation / RNA polymerase II, core complex / transcription elongation by RNA polymerase I / tRNA transcription by RNA polymerase III / protein localization to CENP-A containing chromatin / transcription by RNA polymerase I / Replacement of protamines by nucleosomes in the male pronucleus / Packaging Of Telomere Ends / transcription-coupled nucleotide-excision repair / translation initiation factor binding / Recognition and association of DNA glycosylase with site containing an affected purine / Cleavage of the damaged purine / negative regulation of autophagy / positive regulation of autophagy / telomere organization / Deposition of new CENPA-containing nucleosomes at the centromere / Recognition and association of DNA glycosylase with site containing an affected pyrimidine / Cleavage of the damaged pyrimidine / translesion synthesis / transcription initiation-coupled chromatin remodeling / RNA Polymerase I Promoter Opening / Inhibition of DNA recombination at telomere / Assembly of the ORC complex at the origin of replication / DNA-templated transcription elongation / SUMOylation of chromatin organization proteins / Regulation of endogenous retroelements by the Human Silencing Hub (HUSH) complex / Meiotic synapsis / DNA methylation / Condensation of Prophase Chromosomes / Chromatin modifications during the maternal to zygotic transition (MZT) / SIRT1 negatively regulates rRNA expression / HCMV Late Events

Domain/homology:

Histone-lysine N-methyltransferase, Set2, fungi / Histone-lysine N-methyltransferase (EC 2.1.1.43) family profile. / : / Spt6, S1/OB domain / : / Plus-3 domain / Plus3-like superfamily / Plus-3 domain / Plus3 domain profile. / Short conserved domain in transcriptional regulators. / Leo1-like protein / Leo1-like protein / Spt5 C-terminal nonapeptide repeat binding Spt4 / RNA polymerase II associated factor Paf1 / Paf1 / Cdc73/Parafibromin / RNA polymerase-associated protein Ctr9 / Cell division control protein 73, C-terminal / Cell division control protein 73, C-terminal domain superfamily / RNA pol II accessory factor, Cdc73 family, C-terminal / Set2 Rpb1 interacting domain superfamily / SETD2/Set2, SET domain / Transcription elongation factor 1 / Transcription elongation factor 1 superfamily / Transcription elongation factor Elf1 like / : / Spt6 acidic, N-terminal domain / Helix-turn-helix DNA-binding domain of Spt6 / Transcription elongation factor Spt6, YqgF domain / Transcription elongation factor Spt6, helix-hairpin-helix motif / Spt6, SH2 domain, C terminus / Acidic N-terminal SPT6 / Helix-hairpin-helix motif / Holliday-junction resolvase-like of SPT6 / Helix-turn-helix DNA-binding domain of SPT6 / Tex-like protein, HTH domain superfamily / Tex-like domain superfamily / Spt6, Death-like domain / : / Tex central region-like / Transcription elongation factor Spt6 / Spt6, SH2 domain, N terminus / Spt6, SH2 domain / SH2 domain / YqgF/RNase H-like domain superfamily / Set2 Rpb1 interacting domain / SRI (Set2 Rpb1 interacting) domain / Transcription initiation Spt4 / Spt4 superfamily / TFIIS N-terminal domain profile. / TFIIS helical bundle-like domain / Transcription factor IIS, N-terminal / Spt4/RpoE2 zinc finger / Spt4/RpoE2 zinc finger / Spt4/RpoE2 zinc finger / AWS domain / AWS domain / AWS domain profile. / associated with SET domains / : / Transcription elongation factor SPT5, KOWx domain / Transcription elongation factor SPT5, second KOW domain / Transcription elongation factor SPT5, fifth KOW domain / Transcription elongation factor SPT5, KOW1 domain / Transcription elongation factor SPT5, fourth KOW domain / Transcription elongation factor Spt5, eukaryote / Spt5 transcription elongation factor, N-terminal / Spt5, KOW domain repeat 2 / Spt5, KOW domain repeat 3 / Spt5, KOW domain repeat 5 / Spt5 transcription elongation factor, acidic N-terminal / NGN domain, eukaryotic / Spt5, KOW domain repeat 1 / Spt5, KOW domain repeat 4 / NGN domain / Transcription elongation factor SPT5 / Early transcription elongation factor of RNA pol II, NGN section / Cysteine-rich motif following a subset of SET domains / RuvA domain 2-like / TFIIS/LEDGF domain superfamily / Post-SET domain / Post-SET domain profile. / Tetratricopeptide repeat / NusG, N-terminal domain superfamily / WW domain / WW/rsp5/WWP domain signature. / SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain / WW domain superfamily / WW/rsp5/WWP domain profile. / Domain with 2 conserved Trp (W) residues / SET domain / WW domain / SET domain profile. / SET domain superfamily / SET domain / DNA-directed RNA polymerase II subunit Rpb4-like / RNA polymerase Rpb4/RPC9, core / DNA-directed RNA-polymerase II subunit / RNA polymerase Rpb1 C-terminal repeat / RNA polymerase II, heptapeptide repeat, eukaryotic

Component:

Histone-lysine N-methyltransferase, H3 lysine-36 specific / Transcription elongation factor 1 homolog / DNA-directed RNA polymerase subunit beta / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / Transcription elongation factor SPT4 / Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / RNA polymerase II subunit B32 / Transcription elongation factor SPT5 / RNA polymerase-associated protein LEO1 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / RNA polymerase II subunit B12.5 / DNA-directed RNA polymerase subunit / Component of the Paf1p complex / Transcription elongation factor Spt6 / DNA-directed RNA polymerase II subunit RPB3 / Protein that interacts with Spt6p and copurifies with Spt5p and RNA polymerase II / RNAP II-associated protein / DNA-directed RNA polymerase subunit / RNA polymerase subunit ABC10-alpha / DNA-directed RNA polymerase subunit / RNAPII-associated chromatin remodeling Paf1 complex subunit / Histone H2A type 1-B/E / Histone H2B type 1-J / Histone H4 / Histone H3.3

• Biological species: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus) / Homo sapiens (human) / synthetic construct (others)
• Method: single particle reconstruction / cryo EM / Resolution: 4.28 Å
• Authors: Kujirai T / Ehara H / Ito T / Henmi M / Sekine S / Kurumizaka H

Funding support

Japan, 12 items

Organization	Grant number	Country
Japan Agency for Medical Research and Development (AMED)	JP24ama121009	Japan
Japan Society for the Promotion of Science (JSPS)	JP20H05690	Japan
Japan Society for the Promotion of Science (JSPS)	JP24H00062	Japan
Japan Society for the Promotion of Science (JSPS)	JP20H03201	Japan
Japan Society for the Promotion of Science (JSPS)	JP23K17392	Japan
Japan Society for the Promotion of Science (JSPS)	JP22K15033	Japan
Japan Society for the Promotion of Science (JSPS)	JP24H02328	Japan
Japan Society for the Promotion of Science (JSPS)	JP23H05475	Japan
Japan Society for the Promotion of Science (JSPS)	JP20H05906	Japan
Japan Science and Technology	JPMJER1901	Japan
Japan Science and Technology	JPMJCR24T3	Japan
Other private	Daiichi Sankyo Foundation for Life Science

• Citation: Journal: Sci Adv / Year: 2026; Title: Structural basis of transcription-coupled H3K36 trimethylation by Set2 in coordination with FACT.; Authors: Tomoya Kujirai / Haruhiko Ehara / Tomoko Ito / Masami Henmi / Eriko Oya / Takehiko Kobayashi / Shun-Ichi Sekine / Hitoshi Kurumizaka / ; Abstract: Trimethylation of the histone H3K36 residue (H3K36me3) plays an indispensable role in ensuring transcription fidelity by suppressing undesired cryptic transcription in chromatin. H3K36me3 modification is accomplished by Set2/SETD2 during transcription elongation by the RNA polymerase II elongation complex (EC). Here, we found that Set2-mediated H3K36me3 deposition occurs on the nucleosome reassembling behind the EC. The histone chaperone FACT suppresses H3K36me3 deposition on the downstream nucleosome, thereby ensuring that Set2 targets specifically on the reassembling upstream nucleosome. Cryo-electron microscopy structures of the nucleosome-transcribing EC complexed with Set2 revealed that Set2 is anchored by the Spt6 subunit of the EC to capture both of the H3 N-terminal tails in a stepwise manner during the nucleosome reassembly process. Abrogation of the Set2-EC interaction leads to defective transcription-coupled H3K36me3 deposition. These insights elucidate the structure-based mechanism of transcription-coupled H3K36me3 deposition in chromatin.

History

Deposition	Sep 3, 2025	-
Header (metadata) release	Dec 24, 2025	-
Map release	Dec 24, 2025	-
Update	Mar 4, 2026	-
Current status	Mar 4, 2026	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_66105.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.475 Å

Density

Contour Level	By AUTHOR: 0.014
Minimum - Maximum	-0.027855989 - 0.086293645
Average (Standard dev.)	0.00012860785 (±0.003144143)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 360 360 360 Spacing 360 360 360
Cell	A=B=C: 531.0 Å α=β=γ: 90.0 °

Supplemental data

Additional map: nucleosome reconstruction

• File: emd_66105_additional_1.map
• Annotation: nucleosome reconstruction

Additional map: EC reconstruction

• File: emd_66105_additional_2.map
• Annotation: EC reconstruction

Half map: #2

• File: emd_66105_half_map_1.map

Half map: #1

• File: emd_66105_half_map_2.map

Sample components

Entire : Co-transcriptional histone H3K36 methylation complex containing R...

• Entire: Name: Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp130, type A)

Components

• Complex: Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp130, type A)
  • Protein or peptide: DNA-directed RNA polymerase subunit
  • Protein or peptide: DNA-directed RNA polymerase subunit beta
  • Protein or peptide: RNA polymerase II third largest subunit B44, part of central core
  • Protein or peptide: RNA polymerase II subunit B32
  • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC1
  • Protein or peptide: RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III
  • Protein or peptide: RNA polymerase II subunit
  • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC3
  • Protein or peptide: DNA-directed RNA polymerase subunit
  • Protein or peptide: RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
  • Protein or peptide: RNA polymerase II subunit B12.5
  • Protein or peptide: RNA polymerase subunit ABC10-alpha
  • Protein or peptide: Transcription elongation factor 1 homolog
  • DNA: DNA (198-MER)
  • RNA: RNA (5'-R(P*UP*CP*CP*UP*UP*UP*UP*GP*UP*GP*CP*GP*GP*CP*CP*UP*CP*GP*A)-3')
  • DNA: DNA (198-MER)
  • Protein or peptide: Transcription elongation factor SPT4
  • Protein or peptide: Transcription elongation factor SPT5
  • Protein or peptide: Histone H3.3
  • Protein or peptide: Histone H4
  • Protein or peptide: Histone H2A type 1-B/E
  • Protein or peptide: Histone H2B type 1-J
  • Protein or peptide: Transcription elongation factor Spt6
  • Protein or peptide: Protein that interacts with Spt6p and copurifies with Spt5p and RNA polymerase II
  • Protein or peptide: Component of the Paf1p complex
  • Protein or peptide: RNAPII-associated chromatin remodeling Paf1 complex subunit
  • Protein or peptide: Histone-lysine N-methyltransferase, H3 lysine-36 specific
  • Protein or peptide: Leo1
  • Protein or peptide: RNAP II-associated protein
  • Protein or peptide: Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p
• Ligand: ZINC ION
• Ligand: MAGNESIUM ION
• Ligand: S-ADENOSYL-L-HOMOCYSTEINE

Supramolecule #1: Co-transcriptional histone H3K36 methylation complex containing R...

• Supramolecule: Name: Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp130, type A); type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#30
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)

Macromolecule #1: DNA-directed RNA polymerase subunit

• Macromolecule: Name: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 194.107422 KDa

Sequence

String:

MSQFPYSSAP LRSVKEVQFG LLSPEEIRAI SVVKIEYPEI MDESRQRPRE GGLNDPKLGS IDRNFKCQTC GEGMAECPGH FGHMELAKP VFHIGFIPKI KKVCECICMN CGKLLLDETN PTMAQAIRIR DPKKRFNAVW QLCKTKMVCE ADAPVDEYSE Q KVVSRGGC GNTQPVVRKD GMKLWGTWKK SGFSDRDAQP ERKLLTPGEI LNVFKHISPE DCFRLGFNED YARPEWMIIT VL PVPPPQV RPSIAMDETT QGQDDLTHKL SDILKANINV QKLEMDGSPQ HIINEVEQLL QFHVATYMDN DIAGQPQALQ KSG RPVKAI RARLKGKEGR LRGNLMGKRV DFSARTVISG DPNLELDQVG VPISIAKTLS YPETVTQYNI HRLTEYVRNG PNEH PGAKY VIRDNGDRID LRYHKRAGDI VLQYGWKVER HLMDDDPVLF NRQPSLHKMS MMAHRVKVMP YSTFRLNLSV TSPYN ADFD GDEMNLHVPQ SEETRAELSQ LCAVPLQIVS PQSNKPVMGI VQDTLCGVRK MTLRDTFIEY EQVMNMLFWV PSWDGV VPQ PAILKPKPLW TGKQLLSIAI PSGIHLQRTD GGNSLLSPKD NGMLIVDGKV MFGVVDKKTV GSGGGGLIHT VMREKGP KI CAELFGNIQK VVNYWLLHNG FSIGIGDAIA DASTMKEITH AISSAKEQVQ EIIYKAQHNE LELKPGMTLR ESFEGEVS R TLNDARDSAG RSAEMNLKDL NNVKQMVSAG SKGSFINIAQ MSACVGQQMV EGKRIAFGFA DRSLPHFTKD DFSPESKGF VENSYLRGLT PQEFFFHAMA GREGLIDTAV KTAETGYIQR RLVKALEDIM VHYDGTTRNS LGDIIQFLYG EDGLDGTQVE RQTIDTIPG SDKAFHKRYY VDLMDEKNSI KPDVIEYAAD ILGDVELQKE LNSEYEQLVS DRKFLREIVF VNGDHNWPLP V NLRRIIQN AQQIFHLDRA KASDLTIPEI IHGVRDLCKK LFVLRGENEL IKEAQQNATS LFQCLVRARL ATRRILEEFR LN RDAFEWV LGTIEAQFQR SLVHPGEMVG VIAAQSIGEP ATQMTLNTFH YAGVSSKNVT LGVPRLKEIL NVAKNIKTPA LTV YLDREI ALDIEKAKVI QSSIEYTTLK NVTSATEIYY DPDPTSTVIE EDFDTVEAYF SIPDEKVEET IDKQSPWLLR LELD RARML DKQLTMNQVA DKISEVFSDD LFVMWSEDNA DKLIIRCRVI RDPKAMDEEL EAEEDQMLKR IEAHMLDLIA LRGIP GISK VYMVKHKVSV PDESGEYKNE ELWALETDGI NLAEVMAVPG VDSSRTYSNS FVEILSVLGI EATRSSLYKE ILNVIA FDG SYVNYRHMAL LVDVMTSRGY LMAITRHGIN RADTGALMRC SFEETVEILF EAGAAAELDD CRGVSENVML GQLAPMG TG AFDVMIDEKL LTSLPADYAP TMPLFKGKAT QGSATPYDNN AQYDDEFNHD DVADVMFSPM AETGSGDDRS GGLTEYAG I QSPYQPTSPG LSATSPGFAP TSPGFAPTSP RYSPTSPGYS PTSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPQY SPTSPQYSPT SPQYSPTSPQ YSPTSPQYSP TSPQYSPTSP QYSPTSPQYS PTSPQYSPT SPQYSPTSPQ YSPTSPQYSP TSPQYSPTSP QYSPASPQYS PSRHSPNGES KEGE

UniProtKB: DNA-directed RNA polymerase subunit

Macromolecule #2: DNA-directed RNA polymerase subunit beta

• Macromolecule: Name: DNA-directed RNA polymerase subunit beta / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 139.746094 KDa

Sequence

String:

MSYDPYSIDD TITTEDCWTV ISAFFEEKGL VSQQLDSFDE FMETSIQDLV WEEPRLILDQ PAQHTNEKDN INKRYEIRFG KIYLSRPTM TEADGTTHAM FPQEARLRNL TYSSPVYLDM EKSMFTSIDD EGNPNATLDW QQVHEPIKDG VEEGNKVHIG K VPIMLRSK FCSLRTLDEV DLYKMKECPY DMGGYFVING SEKVLIAQER SAANIVQVFK KAAPSPISHV AEIRSALEKG SR LISTMQI KLYGREDKGT GRTIKATLPY VKQDIPIVIV FRALGVVPDG EILQHICYDE NDWQMLEMLK PCIEEGFVIQ DKE VALDFI GRRGSAALGI RREKRIQYAK DILQKELLPH ITQEEGFETR KTFFLGYMVN RLLLCALERK DQDDRDHFGK KRLD LAGPL LANLFRILFR KLTREIYRYM QRCIETDRDF NLNLAVKSTT ITSGLKYSLA TGNWGEQKKA MSSRAGVSQV LNRYT YSST LSHLRRTNTP IGRDGKLAKP RQLHNTHWGL VCPAETPEGQ ACGLVKNLSL LSGISIGSPS EPIINFLEEW GMEPLE DYD PAQHTKSTRI FVNGVWTGIH RDPSMLVSTM RDLRRSGAIS PEVSIIRDIR EREFKIFTDV GRVYRPLFIV EDDESKD NK GELRITKEHI RKIQQGYDDD AMNDDSEEQE QDVYGWSSLV TSGVIEYVDG EEEETIMIAM TPEDLQTRSL EQKEIDLN D TAKRIKPEMS TSSHHTFTHC EIHPSMILGV AASIIPFPDH NQSPRNTYQS AMGKQAMGVF LTNYNVRMDT MANILYYPQ KPLAKTQAME YLKFRELPAG QNAIVAIACY SGYNQEDSMI MNQSSIDRGL FRSLFFRSYM DQEKRFGISI VEEFEKPTRA TTLRLKHGT YEKLDEDGLI APGVRVSGDD IIIGKTTPIP PDTEELGQRT KYHTKRDAST PLRSTENGIV DQVLLTTNQE G LKFVKVRM RTTKVPQIGD KFASRHGQKG TIGVTYRHED MPFSAEGIVP DLIINPHAIP SRMTVAHLIE CLLSKVGSIR GY EGDATPF TDLTVDAVSN LLRDNGYQSR GFEVMYNGHT GKKLMAQVFF GPTYYQRLRH MVDDKIHARA RGPVQVLTRQ PVE GRSRDG GLRFGEMERD CMIAHGAAGF LKERLMEASD AFRVHVCGIC GLMSVIANLK KNQFECRSCK NKTNIYQLHI PYAA KLLFQ ELMAMNIAPR LYTERSGVSM RS

UniProtKB: DNA-directed RNA polymerase subunit beta

Macromolecule #3: RNA polymerase II third largest subunit B44, part of central core

• Macromolecule: Name: RNA polymerase II third largest subunit B44, part of central core; type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 34.216293 KDa

Sequence

String:

MSKEPKVNII NAQDDEVELM LSDVNLSLAN SLRRTMLAEV PTLAIDLVEI KMNTSVLADE FISHRLGLIP LVSEDVEEMK YSRDCTCED YCDECSVVLE LSARHEGEEG TTDVYSSSLI KVSGPGNLNV GEPVRRDDYD QGILLCKLRN HQELNIRCIA K KGIAKEHA KWSPCSAIAF EYDPHNKLKH TDFWFEVDAK KEWPDSKYAT WEEPPKPGEV FDYKAKPNRF YMTVETTGSL KA NQVFSRG IKTLQEKLAN VLFELENSRP ANTTAYGGAT AYGGQTVYGR ETSYGGNTNY GDYNAPY

UniProtKB: DNA-directed RNA polymerase II subunit RPB3

Macromolecule #4: RNA polymerase II subunit B32

• Macromolecule: Name: RNA polymerase II subunit B32 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 20.62298 KDa

Sequence

String:

MNVSTSTVGA RRRRAKQQVD DEENATLLRL GPEFALKQYD HDGNEHDLIA LSLSESRLLI REALKARSRA RNGGVDIESS NGEIDDDEL AKVTSGAVAN GVVKKTLDYL NTFARFKDEE TCTAVDQLLH NSSDCSVLHP FEIAQLSSLG CEDVDEAITL I PSLAAKKE VNLQRILDEL NRLEDPYK

UniProtKB: RNA polymerase II subunit B32

Macromolecule #5: DNA-directed RNA polymerases I, II, and III subunit RPABC1

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC1; type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 24.96268 KDa

Sequence

String:

MEDNNRIISR LWRSFRTVKE MAADRGYFIS QEEMDQSLEE FRSKICDSMG NPQRKLMSFL ANPTPEALEK YSDLGTLWVE FCDEPSVGI KTMRNFCLRI QEKNFSTGIF IYQNNITPSA NKMIPTVSPA IIETFQESDL VVNITHHELV PKHIRLSDGE K SQLLQRYK LKESQLPRIQ REDPVARYLG LKRGQVVKII RRSETSGRYA SYRICL

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC1

Macromolecule #6: RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III

• Macromolecule: Name: RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 17.803588 KDa

Sequence

String:

MSEDEAFNEQ TENFENFEDE HFSDDNFEDR STQPEDYAVG VTADGRQIIN GDGIQEVNGT IKAHRKRSNK ELAILKEERT TTPYLTKYE RARILGTRAL QISMNAPVLV DIEGETDPLQ IAMKELSQRK IPLVIRRYLP DGSYEDWGCD ELIVDN

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC2

Macromolecule #7: RNA polymerase II subunit

• Macromolecule: Name: RNA polymerase II subunit / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 18.802625 KDa

Sequence

String:

MFFLKDLSLI LTLHPSYFGP QMNQYLREKL LTDVEGTCTG QFGYIVTVLD GMNIDVGKGR IIPGSGSAEF EVKYRAVVWK PFKGEVVDA IVSNVSPIGF FADVGPLNVF VSTRLIPDNL VYNPSNSPPA YMSNDELITK GSKVRLKVVG TRTDVNEIYA I GSIKEDFL GAI

UniProtKB: DNA-directed RNA polymerase subunit

Macromolecule #8: DNA-directed RNA polymerases I, II, and III subunit RPABC3

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC3; type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 16.24922 KDa

Sequence

String:

MSSALFDDIF TVQTVDNGRY NKVSRIIGIS TTNSAIKLTL DINNEMFPVS QDDSLTVTLA NSLSLDGEDE SANFSKSWRP PKPTDKSLA DDYDYVMFGT VYKFEEGDED KIKVYVSFGG LLMCLEGGYK SLASLKQDNL YILIRR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC3

Macromolecule #9: DNA-directed RNA polymerase subunit

• Macromolecule: Name: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 13.61232 KDa

Sequence

String:

MASFRFCLEC NNMLYPKEDK ENQRLLYSCR NCDYTELAED PKVYRHELIT NIGETAGIVD DIGQDPTLPR SDKECPECHS RDCVFFQSQ QRRKDTNMTL FYVCLNCKKT FRDESE

UniProtKB: DNA-directed RNA polymerase subunit

Macromolecule #10: RNA polymerase subunit ABC10-beta, common to RNA polymerases I, I...

• Macromolecule: Name: RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III; type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 8.554064 KDa

Sequence

String:

MIIPVRCFSC GKVVGDKWDA YLRLLEEGKQ EGDALDELKL KRYCCRRMVL THVDLIEKFL RYNPLEKKDF DS

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC5

Macromolecule #11: RNA polymerase II subunit B12.5

• Macromolecule: Name: RNA polymerase II subunit B12.5 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 13.832896 KDa

Sequence

String:

MNAPDRFELF ILPDDVPKLK ITPDSRVPNC IIIKFEREDH TLANLLREEL ALYPDVTFVA YKVEHPLFAN FVMRLQTEEG TRPKQALER ACASIINKLK TLDHKFNEEW NIKNFSLND

UniProtKB: RNA polymerase II subunit B12.5

Macromolecule #12: RNA polymerase subunit ABC10-alpha

• Macromolecule: Name: RNA polymerase subunit ABC10-alpha / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 7.862048 KDa

Sequence

String:

MSREGFVAPS GTDLAAAASG VAPNKHYGVK YTCGACAHNF SLNKSDPVRC KECGHRVIYK ARTKRMIQFD AR

UniProtKB: RNA polymerase subunit ABC10-alpha

Macromolecule #13: Transcription elongation factor 1 homolog

• Macromolecule: Name: Transcription elongation factor 1 homolog / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 12.606896 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPGMGKRKSS ARKPAPKIKQ KLETQFTCLF CNHDNSVVCT LDKKNSIGLL ECKKCNLSFQ APINSLSQPI DIYSDWIDAC EAVAEENAD VNGDNFIEND GADREQDDDY DDEF

UniProtKB: Transcription elongation factor 1 homolog

Macromolecule #17: Transcription elongation factor SPT4

• Macromolecule: Name: Transcription elongation factor SPT4 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 12.039614 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MRERACMLCG IVLPGRVFMQ NGCPNCDSVL NLRDSDQATV NECTSSSFEG LVAVGDNEHS WVAKWLRVDR FQPGLYAVRV DGRLPSDIV AALEQYGVYY RPRDGSVID

UniProtKB: Transcription elongation factor SPT4

Macromolecule #18: Transcription elongation factor SPT5

• Macromolecule: Name: Transcription elongation factor SPT5 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 101.459422 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPGMSETHKN QLDKVSTVSP DGPSEAVKEH SLQSKDLSKN DGQFIVPLDR NVIEQEEHKQ VKSSAQAHNT TGDAADNEIE DGVPSEDVE FDKFKEDDYD EDDEVEEEGD IRSRKRRRHN QFLDVEAEVD DEEDDDDDDD DVELKHDFIQ DDHIQHETQN E GFIAGHVD DDRLHRKLDQ SREKIADQDA QELADEFKQR YGRSASSKYM GSASTTAPQR LLIPTVDDPG IWGVKVRLGK EK DVVRQIL KKKLAREGTK NPLEIYSAFQ RDSFKGHVYI EARKAEAIND ALKGNVNVFS NNSKFLVGIV EYKDLLRPVK SSD VKLTRG SYVRVKNGKF KGDLAQVDEV LENGLEARLK LVPRLDYGKD LSHLSTSSSV DSTKNRRKFY TSKFRPAQRL FSEA EARVH EPTIRRDRDG FVTYGGEEYY EGFLYKTFRL QNLIVNSINP TLNELSLFQS NEESTTIDLS TIADSLKETA KNLVS FQPG DNVEIINGEL NHLTGTVSSV NQSTIVSVRL HSDDDTINSE TVEIPTSDLR KIFNVGDHVR VIHGKHTDDT GLIVEV NGD KVEFISNQTK RTVIVFSNYL IKSTDSTVSI NESGRFELHD LVQVNSDLVG IVIRAQKDSF DVLCSDGKLL SLPPVSI YS KLNLNPNQQI AIDSNGVEVK VGDTVREFTG ERRQGTILHV YRNFLFLRSR EIVENQGVFV TSSNRVKTIT SKSNGTGG Q ISGPDLSRMN PSRVIPPPSI PVANQRMTGR DPTLNKTVKI RQGGYKGKIG IVKEANGDRF RVELHNPNKT IPIPCSFLL IESTHGWVPY EDFVASDRRG GNIPRHEISG HVQQPQHGRA PAWGSGGKTP AWGSGGKTPA WGSGGSGGKT PAWGSGGKTP TWGSGAKTP AWGSGSKTPA WSGLDEEDRR DF

UniProtKB: Transcription elongation factor SPT5

Macromolecule #19: Histone H3.3

• Macromolecule: Name: Histone H3.3 / type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.645277 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GSHMARTKQT ARKSTGGKAP RKQLATKAAR KSAPSTGGVM KPHRYRPGTV ALREIRRYQK STELLIRKLP FQRLVREIAQ DFKTDLRFQ SAAIGALQEA SEAYLVGLFE DTNLCAIHAK RVTIMPKDIQ LARRIRGERA

UniProtKB: Histone H3.3

Macromolecule #20: Histone H4

• Macromolecule: Name: Histone H4 / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 11.676703 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GSHMSGRGKG GKGLGKGGAK RHRKVLRDNI QGITKPAIRR LARRGGVKRI SGLIYEETRG VLKVFLENVI RDAVTYTEHA KRKTVTAMD VVYALKRQGR TLYGFGG

UniProtKB: Histone H4

Macromolecule #21: Histone H2A type 1-B/E

• Macromolecule: Name: Histone H2A type 1-B/E / type: protein_or_peptide / ID: 21 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.447825 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GSHMSGRGKQ GGKARAKAKT RSSRAGLQFP VGRVHRLLRK GNYSERVGAG APVYLAAVLE YLTAEILELA GNAARDNKKT RIIPRHLQL AIRNDEELNK LLGRVTIAQG GVLPNIQAVL LPKKTESHHK AKGK

UniProtKB: Histone H2A type 1-B/E

Macromolecule #22: Histone H2B type 1-J

• Macromolecule: Name: Histone H2B type 1-J / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.217516 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GSHMPEPAKS APAPKKGSKK AVTKAQKKDG KKRKRSRKES YSIYVYKVLK QVHPDTGISS KAMGIMNSFV NDIFERIAGE ASRLAHYNK RSTITSREIQ TAVRLLLPGE LAKHAVSEGT KAVTKYTSAK

UniProtKB: Histone H2B type 1-J

Macromolecule #23: Transcription elongation factor Spt6

• Macromolecule: Name: Transcription elongation factor Spt6 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 173.241625 KDa
• Recombinant expression: Organism: Spodoptera frugiperda (fall armyworm)

Sequence

String:

GPGMSAPSPS VSEEDINETR NSIDADASTV QDLLDQDAQE GSGSDNEGSN IVDSSEEDDD EDDEEEMQKV REGFIVDDDD ENDEDGIPS STKRKHRKKH KKRSAEVVEE VDEDDLQLLM ENSGAVPGQQ QNVKFKRLKR AEQDEKAQDS DSRGLNDMFS D EEGPGGVV EEGSEEEGLE DNLTTKTQRS GNLPHDEFDD FIEEDEFSDE DDEARDERLA RMRSAKAAPQ LAGLQGEQYQ EI IEIFGDG TDYQWTLDAE EEMEQPQQDQ EYDEAGEEIK GTTTSLADVF EPSELKEKML TDEDNVIRVT DLPERFQAYR KSI KNYKLS DVDYSNERDW IVEQLKLEKR DFLQHLTQAH SSVAHLEEKF EASVKKIVDF IAIESFEVPF IWNHRRDYAL HTYN DDSNN TIIVKLLNED DLWRIVQLDL DYHSIHDKKA ALSSIYKQLD LDVVDPTYEE FFGSARTLSE LQDIDDYLTF NYSSQ VKNL TAVAELSIEG NGSGEDEEQT TKSSFAEVKM KRKYSKYAIY DRIRQDAIYP VVQSIANISQ MRENLAQSKR LHQVED PIE SPMDMIADIM STEKDKTTFI SSEKAYQAVK QFFSEQLSYE PFIRKTIRTA FQSFGVINIE LTERGKLQIE PESPYFD FK YAKNRPISAL TATPDLYLRM IQAENDGLVN IKVELPMLST VVDHFYNILK SDGTSEISEK WNALRNDAWK QSLDKLIP L VQLNVKESIR RDCERVLYFQ VKNSFTKKID QAPYQPPTYA KGTIPRVLTL SFGEGNRGDA VLGVFMDDSG DVKSQIKFD EDFQSRDFSD SLTRYIKSNN INPDIIGISG FNIHTKKLFD KVNELVNEER LTIEYDNEYG YDREEDGRSD KHLIRVIYVN DETARLYQH SSKSSAEYPN RPQLAKYCIG LAKYIQSPLL EYLALDESMY SLHIHKHQNL LPREKLIDAV QTSIVDIVNL V GVDINEAV RAPYHALALP YVCGLGPRKA AGLIQSIQRI GSNLVNRAHL ITEQLTSKTV FLNMASFVYI VFDPDVERNP QG EMDLLDS TRIHPEDYSL ARKMAADALD IEDIDDDDES AMRNAIYEMV FPRSPPKDED DLTFKLDELI LDDYATELER KHQ LKKRST LQIIKEELQS RYREIRRDFH ILNEAEIFQL LTRETVDSFR KGMVIPVYVR KVESSYMSVS TQSLIAGNIQ RQDI LEPND RRDPREVYSV GQTVRACILD VDYYNFKCQL SLLRQFTENQ VAGLNVNRNP KFWDIESENR DRQEEIDKQR EESRE SRVI KHPFFHNMKS KEAEDYLAAR PVGDVVIRPS SKGSNHITIS WKVAPQLYQH IDVLEENKDD ANAIGRVLLV GKYRYH DLD ELLVEYVNNV ANKVELMVSH DKFMSDSLDY VKEWLERYSK ANGNRSHYIF TFNRKAPGWF FLLFKLNPTS EIKIWNV KA LPDGYLLANN VYPDTNSLCN GFKTLMSSRR QIKQRSNRAG GEYNNSHAGA YDNGYSNAPR Y

UniProtKB: Transcription elongation factor Spt6

Macromolecule #24: Protein that interacts with Spt6p and copurifies with Spt5p and R...

• Macromolecule: Name: Protein that interacts with Spt6p and copurifies with Spt5p and RNA polymerase II; type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 46.907785 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPGMSDEESE NKKLLDQVLP EKTDQETQPE ADAALAEQEA PENEPNEPAS DDDSDSDLSD ISDVDEEKVA EGLRYLDEQT AASLSKHKA AQPSSTHKKK EPTRRRPKQQ TARETQYEPD VVEDEAARRT RLFEEKLDAA IKRKPNKRKK NDDVDLEQMQ D ELIQQLKL QMEESAIRDA NNIEQGKPAI FKLKLLPKVK DILLRANLAD SILDNNLLAS VRLWLEPLPD ASLPAYQIQK VL FDAIKSL PIKTSHLRES GLGKVMVFYQ KSKRVEPNLK RTAEKLISDW TRPIMGASDN YKDMRVRTQQ FDPAQFAESL PGR VSVRPQ EAKTLYEEAA ERRKRAAIPQ ARTAAYTIAP QVNTELLMSS ARRTLPSGVG SSLSGEDQYK RLNSRLNTMG TKRK SSAKK GGISIEGRGL PQ

UniProtKB: Protein that interacts with Spt6p and copurifies with Spt5p and RNA polymerase II

Macromolecule #25: Component of the Paf1p complex

• Macromolecule: Name: Component of the Paf1p complex / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 124.979008 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MKDHLIHNHH KHEHAHAEHD YKDDDDKEHL YFQGSSGSSG MSLLDQDYYL SALPEDKRAE LVTLQLTIPL KSGDEVVTID FNEDVDVSQ LCVLLESENA RPDLWLSAAK VFVSKGNIAG SQEIIRKALQ SNVILDSSAS VTSLFHNFSF WLSLMEYVST N SREDQFLE YASNSLQASN SLDSGLILNG IGNGVLYAKS RRYDEALKEF DNLLKKKSTN ILAILGKAQI LYKRQKYSQA LE LYQKALT INPLIVPDPR LGIGLCFWHL NNKQLAEQAW HNSLKVHPQN NLNTKILICL AKFDYCFNES KDDDEFTALY RES LEFLHS CLKEDAKHPL LLMVLASYYF SKEDYEKVEK LCNLVLKENS RNAAFVSASH FWLARVAYHK EDYVQAQKQF KQAE DSQNS NTLAKLGYAQ CLIARNEVGD ATIYLEKFFK ENQDSKSSEM MLLLGIIYSQ SGKSYYKAII FLEKYVAVCQ EENYP ILPE AYLVLSRVYE NKDLNVALDY LMKANDILGD KANVYVLNNL GIYHFFRNNV SQSSDFFAQS LEALNNVSPQ NKEALS ITL HYNKARVEEV SNQSEAEKLY SKLMEKCPGY TSNKIRYIYL LALKSNGNNY ADVQQLLDDF PSDLEVRSFY GWFLKRY GR KNGLKQDLES QHHKDTLINY DKHDCYALLS LGNIYATIAR EMKVTDQKQN EIKRQQYLRA AQFYHKVLSI DPKNIYAA Q GIAIIFSDKE RTGLALEIFK KVRDTVQDLG TFINLGHCFM EAKQFGKAIE SYTIALEKFS NGMDSKLLVL IGRAWYHRG FYEKSMDAYK KALEVSEQAY QLSKLPALRF NIVFIQFQIA DFVKSLPNTQ RDLTTLENAL SGLNDAIKSL LKLAELEQPP YPSEDLKAR ATMGSNTLRN QLERAIQDQQ DYEMSIQEKL RTARRKQQLD EEKRLEQEQR RLEEARKRQE AELIKRQELI K QAEEWNKM DIDAAKDNND VLSEDGGEGE KKTTKKRKRK QKVSEDIAPD LDRINEENGN TEDYDPILDN EEEENYDEKD SE NKENGQN GQNEEVYPES EGDDEDDVTN TKRQKRSKGI VDEEDSE

UniProtKB: Component of the Paf1p complex

Macromolecule #26: RNAPII-associated chromatin remodeling Paf1 complex subunit

• Macromolecule: Name: RNAPII-associated chromatin remodeling Paf1 complex subunit; type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 62.301246 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MKDHLIHNHH KHEHAHAEHL YFQGSSGSSG MSDKEQDLDE DLLALAGAGS DSESNSAYSP EQEVSRKSKK IVNDSEDENN DSDGEQLLN PYPLEDKFKD EADRARLLGL SEMEREAILY ERSQEVIKLK EKHLLSLRAK QSQLEKTARE GPKSSKTNKL S ELKKQREQ KSKRQRSDEY SDEEDDYRYE EVGDNDDVEM SDNYIDEDDA VFVEPKKSSR SQELSKPATL SDINKIIFGR TA MSKYWYY PEFDDVVKGM YLRLNTGSGG NGFSPYKVVE VLGSQRIKGS AYGLNSKENN CDMYLKVAFP NQKEMVRPLF VFS DSSITH PEFDLFLREL DAEGLSVMDL RDVDYKYHQL KEMSSRSLSN DEVNSIVKMK QSLSSNTGFN TVLKKAQLQE ELEE ARDAH DHERVARIEA ELKSIGAESV VASKASSSML KIDQRNKKLN NRFIRKAEMA AVEKRKLRGT SESDPFYRLA TTARM FYKS ANLGADDSNQ PKTKAELEEE AKQEKKLEMQ KLESMVKSNY RNGGLDRIIS KIDFDFDLEL

UniProtKB: RNAPII-associated chromatin remodeling Paf1 complex subunit

Macromolecule #27: Histone-lysine N-methyltransferase, H3 lysine-36 specific

• Macromolecule: Name: Histone-lysine N-methyltransferase, H3 lysine-36 specific; type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO / EC number: [histone H3]-lysine36 N-trimethyltransferase
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 83.724547 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPGMRERTVS DEEVDNYGHA SHFKRKVQIY DYPADKTEEA LTTFKTLSQN SYQNKTLGNA HNGRSTADGM SCDCEENWDT VSGINNACG EYSECINRLT SIECISGTCS CGDDCQNQRF QKKQYAPIAV FETEKKGYGV RAQADIRQDA FIYEYLGEVI D ESTFRKRK ENYDNQGLEH FYFMMLQKGE FIDATAKGGL GRFCNHSCRP NAYVDKWEVG NKLRMGIFAK REIYKGEEIC FD YNVDRYG ANPQKCYCGE DNCIGFLGGR TQTDSANILP VPIAEALGST NAQEQRWVRL MKEQNKSIKA SDYSSINEDY VNS LEMRPI ADKDEASKVT SALLKTQDYI ILRKLIERVS LTKDPEVLKE IVRLRGYQCV ANILTMIVLD QEQQQVDVES FTLS VLDML ESWPNSSRNR ISSSQIESVL ESVKTKLKKI QPIGKKINSL LNSWSKLEIS YKIPKSQSVG ASNDVDNGHA ESSTF QLRD EQKENNAASK ISPFRIVDNM LLPEQWRWAV DESTGANYYY NKSQNITQWD LPPGCELLNI EVSEKNRDKK PPIDKS RLE LKERQRKQEY ERVKKERERE KLRLQREQEQ EEQRRTETLK KIIANAEAKQ QLLSKRLADE ENEGKKRHKK SKRPTRE QE WNKLFATVVP NMIKKHESQI GRDNLKHCAK DIVHQLSSKE IKKDTSKKPP KELSSEKKAK VKAFVGPYME KYLDKWKA K HEKK

UniProtKB: Histone-lysine N-methyltransferase, H3 lysine-36 specific

Macromolecule #28: Leo1

• Macromolecule: Name: Leo1 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 52.387715 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MKDHLIHNHH KHEHAHAEHL YFQGSSGSSG MSQELEQNRH SGEESPDPVL DANTSDVDDD GLDLFGDDDD EQPEAKPGQL DDSSNDAEE DEQEKAGKPE AKVTSHTKSV YDNGETLDIS LPIHPKSHIP QGKQDRWVVK LPDFLDINAE PFDPRPFEMN V KTHEDKNQ ELLDKLIAVN TVRWRYAKSE TGGIFKETNS QIIQWEDGTY SLRVGSEIFD MFTTNTDDNY LVSEHNEEGI LM TESTLSK SVKLVPASFQ STTHQKLAKA LSAKQKKESY ARSVVTKEDP EERQRRLESQ ENERYRLERR RKQAEEEEDY DSY AVSTSR SRPSRASQMY NAADEDDDDD DVAIGRTLAS RRNNGYEDDG FIIDDEEEEE AVENDSADEE SENEGDDDEE DDEE AAERL NRLKRAGASK YTNEGEQAQS EPVKSEEADN ASQRDDAPRK KRRVILDDDE EEEE

UniProtKB: RNA polymerase-associated protein LEO1

Macromolecule #29: RNAP II-associated protein

• Macromolecule: Name: RNAP II-associated protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 46.04598 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GSAMTSSKSR QDYIAKVRYQ NDLPAPPCPP KLLKYEIEKE APQKEFLKDS RLLSALFSKD NFRYLMNETS DGLDVNYLRI PGIIENEKS LGKLFSSYKN LAIENLHPDD RLLLVDPNKS ATLNEESPVF FLRRPQYVSD GEKINLQNFT NKRKHQDMED T NPRSQLHS VERTFDEVID PRNKNRLQSL IHPRKKIKAV KAWHFFPDTS TFDQVFHSLK FVGSASLSKD RPLNEQLGQV SE TDSTSVN ASILTSLFKP IEINPHNKWI SLYAVTDKLS AESFRKSFNS IKDDNIVNRH VIYDHIKDFD QMFRGHKKLF EDF AISFDD ISDRAFFVPI VGRLELKKKR IVPGLVDMVN RTNYAHIRMD LRNPSTQETA IRDSRREQYD PVNYSSIQEE

UniProtKB: RNAP II-associated protein

Macromolecule #30: Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p,...

• Macromolecule: Name: Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p; type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (fungus)
• Molecular weight: Theoretical: 44.760652 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MKDHLIHNHH KHEHAHAEHL YFQGSSGSSG MSEPLIALRQ AIKDKKPVGV NEGETIGNAK VLNIGEKQYS LDAPTSFVIN GKEFNLKVI YQCWVFRDSS SADYITECEK ENIDGISFVE RSELISWLKG EITSSAFIKG EKVGITEENG KNEGETKSNN K RKLSDDPL LKEIASNERV LIDHNKVLRG LKPKDFSSVA KDCELRILKE KPANAKSSDS NGRSSTSVSS SSKDARNKEP II VLSPAAS SLVRMSNVKE FLQEGKFLDP SKEPASSSNL LAIQRKSSRF KTPIKLLVVD NVEKLFTKSE YWDRVVAIVT TGK DWQFKN YKYKDPQILF QKFNGFYFKY KGDAVPASVK SWNVKVLDID RVERFSDRQV VEQFWDTVEN TLVAKRYKS

UniProtKB: Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p

Macromolecule #14: DNA (198-MER)

• Macromolecule: Name: DNA (198-MER) / type: dna / ID: 14 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 61.25168 KDa

Sequence

String:

(DG)(DC)(DT)(DT)(DA)(DC)(DG)(DT)(DC)(DA) (DG)(DT)(DC)(DT)(DG)(DG)(DC)(DC)(DA)(DT) (DC)(DT)(DT)(DT)(DG)(DT)(DG)(DT)(DT) (DT)(DG)(DG)(DT)(DG)(DT)(DG)(DT)(DT)(DT) (DG) (DG)(DG)(DT)(DG)(DG)(DT)(DG)(DG) (DC)(DC)(DG)(DT)(DT)(DT)(DT)(DC)(DG)(DT) (DT)(DG) (DT)(DT)(DT)(DT)(DT)(DT)(DT) (DC)(DT)(DG)(DT)(DC)(DT)(DC)(DG)(DT)(DG) (DC)(DC)(DT) (DG)(DG)(DT)(DG)(DT)(DC) (DT)(DT)(DG)(DG)(DG)(DT)(DG)(DT)(DT)(DT) (DT)(DC)(DC)(DC) (DC)(DT)(DT)(DG)(DG) (DC)(DG)(DG)(DT)(DT)(DT)(DT)(DT)(DT)(DC) (DG)(DC)(DG)(DG)(DG) (DG)(DG)(DT)(DC) (DT)(DG)(DC)(DG)(DC)(DG)(DT)(DT)(DC)(DG) (DT)(DG)(DC)(DG)(DT)(DT) (DT)(DT)(DT) (DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DT)(DG) (DT)(DG)(DC)(DT)(DG)(DT)(DC) (DT)(DT) (DC)(DG)(DT)(DC)(DC)(DT)(DT)(DT)(DT)(DG) (DT)(DG)(DC)(DG)(DG)(DC)(DC)(DT) (DC) (DG)(DA)(DA)(DA)(DA)(DC)(DG)(DG)(DG)(DA) (DT)(DT)(DC)(DT)(DG)(DA)(DT)

Macromolecule #16: DNA (198-MER)

• Macromolecule: Name: DNA (198-MER) / type: dna / ID: 16 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 60.997227 KDa

Sequence

String:

(DA)(DT)(DC)(DA)(DG)(DA)(DA)(DT)(DC)(DC) (DC)(DG)(DT)(DT)(DT)(DT)(DC)(DG)(DA)(DG) (DG)(DC)(DC)(DG)(DC)(DA)(DC)(DA)(DA) (DA)(DA)(DG)(DG)(DA)(DC)(DG)(DA)(DA)(DG) (DA) (DC)(DA)(DG)(DC)(DA)(DC)(DA)(DA) (DG)(DC)(DA)(DC)(DC)(DG)(DC)(DA)(DA)(DA) (DA)(DA) (DC)(DG)(DC)(DA)(DC)(DG)(DA) (DA)(DC)(DG)(DC)(DG)(DC)(DA)(DG)(DA)(DC) (DC)(DC)(DC) (DC)(DG)(DC)(DG)(DA)(DA) (DA)(DA)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA) (DA)(DG)(DG)(DG) (DG)(DA)(DA)(DA)(DA) (DC)(DA)(DC)(DC)(DC)(DA)(DA)(DG)(DA)(DC) (DA)(DC)(DC)(DA)(DG) (DG)(DC)(DA)(DC) (DG)(DA)(DG)(DA)(DC)(DA)(DG)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DC)(DA) (DA)(DC)(DG) (DA)(DA)(DA)(DA)(DC)(DG)(DG)(DC)(DC)(DA) (DC)(DC)(DA)(DC)(DC)(DC)(DA) (DA)(DA) (DC)(DA)(DC)(DA)(DC)(DC)(DA)(DA)(DA)(DC) (DA)(DC)(DA)(DA)(DG)(DA)(DG)(DC) (DT) (DA)(DA)(DT)(DT)(DG)(DA)(DC)(DT)(DG)(DA) (DC)(DG)(DT)(DA)(DA)(DG)(DC)

Macromolecule #15: RNA (5'-R(P*UP*CP*CP*UP*UP*UP*UP*GP*UP*GP*CP*GP*GP*CP*CP*UP*CP*GP...

• Macromolecule: Name: RNA (5'-R(P*UP*CP*CP*UP*UP*UP*UP*GP*UP*GP*CP*GP*GP*CP*CP*UP*CP*GP*A)-3'); type: rna / ID: 15 / Number of copies: 1
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 5.984529 KDa

Sequence

String:

UCCUUUUGUG CGGCCUCGA

Macromolecule #31: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 31 / Number of copies: 13 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #32: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 32 / Number of copies: 1 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #33: S-ADENOSYL-L-HOMOCYSTEINE

• Macromolecule: Name: S-ADENOSYL-L-HOMOCYSTEINE / type: ligand / ID: 33 / Number of copies: 1 / Formula: SAH
• Molecular weight: Theoretical: 384.411 Da

Chemical component information

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 59.1 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.2 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Type: NONE
• Startup model: Type of model: OTHER
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.28 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 15255
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD