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- EMDB-65946: Focused map of pocket engineered OR52cs in its active state -

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Basic information

Entry
Database: EMDB / ID: EMD-65946
TitleFocused map of pocket engineered OR52cs in its active state
Map dataunsharpened main map
Sample
  • Complex: Complex of pocket engineered OR52cs and hetertrimeric Gs and Nb35
KeywordsOdorant receptor / MEMBRANE PROTEIN / MEMBRANE PROTEIN-IMMUNE SYSTEM complex
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.29 Å
AuthorsChoi CW / Choi H-J
Funding support Korea, Republic Of, 2 items
OrganizationGrant numberCountry
Samsung Science and Technology Foundation Korea, Republic Of
National Research Foundation (NRF, Korea) Korea, Republic Of
CitationJournal: To Be Published
Title: The structure of pocket engineered OR52cs in its active state
Authors: Choi CW / Choi H-J
History
DepositionAug 22, 2025-
Header (metadata) releaseJun 17, 2026-
Map releaseJun 17, 2026-
UpdateJun 17, 2026-
Current statusJun 17, 2026Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_65946.png

Downloads & links

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Map

FileDownload / File: emd_65946.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationunsharpened main map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.82 Å/pix.
x 320 pix.
= 263.936 Å		0.82 Å/pix.
x 320 pix.
= 263.936 Å		0.82 Å/pix.
x 320 pix.
= 263.936 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.8248 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.27
Minimum - Maximum-1.0699124 - 1.6662782
Average (Standard dev.)-0.0026149822 (±0.02738695)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 263.936 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: sharpened additional map

Fileemd_65946_additional_1.map
Annotationsharpened additional map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: half map A

Fileemd_65946_half_map_1.map
Annotationhalf map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: half map B

Fileemd_65946_half_map_2.map
Annotationhalf map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Complex of pocket engineered OR52cs and hetertrimeric Gs and Nb35

EntireName: Complex of pocket engineered OR52cs and hetertrimeric Gs and Nb35
Components
  • Complex: Complex of pocket engineered OR52cs and hetertrimeric Gs and Nb35

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Supramolecule #1: Complex of pocket engineered OR52cs and hetertrimeric Gs and Nb35

SupramoleculeName: Complex of pocket engineered OR52cs and hetertrimeric Gs and Nb35
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.03 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.29 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 84454
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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