ジャーナル: Science / 年: 2016 タイトル: Isolation of potent neutralizing antibodies from a survivor of the 2014 Ebola virus outbreak. 著者: Zachary A Bornholdt / Hannah L Turner / Charles D Murin / Wen Li / Devin Sok / Colby A Souders / Ashley E Piper / Arthur Goff / Joshua D Shamblin / Suzanne E Wollen / Thomas R Sprague / ...著者: Zachary A Bornholdt / Hannah L Turner / Charles D Murin / Wen Li / Devin Sok / Colby A Souders / Ashley E Piper / Arthur Goff / Joshua D Shamblin / Suzanne E Wollen / Thomas R Sprague / Marnie L Fusco / Kathleen B J Pommert / Lisa A Cavacini / Heidi L Smith / Mark Klempner / Keith A Reimann / Eric Krauland / Tillman U Gerngross / Karl D Wittrup / Erica Ollmann Saphire / Dennis R Burton / Pamela J Glass / Andrew B Ward / Laura M Walker / 要旨: Antibodies targeting the Ebola virus surface glycoprotein (EBOV GP) are implicated in protection against lethal disease, but the characteristics of the human antibody response to EBOV GP remain ...Antibodies targeting the Ebola virus surface glycoprotein (EBOV GP) are implicated in protection against lethal disease, but the characteristics of the human antibody response to EBOV GP remain poorly understood. We isolated and characterized 349 GP-specific monoclonal antibodies (mAbs) from the peripheral B cells of a convalescent donor who survived the 2014 EBOV Zaire outbreak. Remarkably, 77% of the mAbs neutralize live EBOV, and several mAbs exhibit unprecedented potency. Structures of selected mAbs in complex with GP reveal a site of vulnerability located in the GP stalk region proximal to the viral membrane. Neutralizing antibodies targeting this site show potent therapeutic efficacy against lethal EBOV challenge in mice. The results provide a framework for the design of new EBOV vaccine candidates and immunotherapies.
全体 : Reconstruction of Ebola virus GP ectodomain containing mucin-like...
全体
名称: Reconstruction of Ebola virus GP ectodomain containing mucin-like domains in complex with human survivor antibody Fab ADI-15731
要素
試料: Reconstruction of Ebola virus GP ectodomain containing mucin-like domains in complex with human survivor antibody Fab ADI-15731
タンパク質・ペプチド: Ebola virus glycoprotein
タンパク質・ペプチド: Immunoglobulin 1 fragment
-
超分子 #1000: Reconstruction of Ebola virus GP ectodomain containing mucin-like...
超分子
名称: Reconstruction of Ebola virus GP ectodomain containing mucin-like domains in complex with human survivor antibody Fab ADI-15731 タイプ: sample / ID: 1000 / 集合状態: One GP trimer bound to three Fab monomers / Number unique components: 2