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- EMDB-65626: Fusion protein of Helicoverpa armigera nucleopolyhedrovirus in an... -

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Basic information

Entry
Database: EMDB / ID: EMD-65626
TitleFusion protein of Helicoverpa armigera nucleopolyhedrovirus in an early fusion intermediate state
Map data
Sample
  • Complex: Membrane fusion protein of Helicoverpa armigera nucleopolyhedrovirus
    • Protein or peptide: fusion protein
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
Keywordsfusion protein / Baculovirus / membrane fusion / VIRAL PROTEIN
Biological speciesHelicoverpa armigera nucleopolyhedrovirus
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsChong T / Rao G / Fu Y / Cao S
Funding support China, 1 items
OrganizationGrant numberCountry
Ministry of Science and Technology (MoST, China)2022YFC2303300 China
CitationJournal: To Be Published
Title: Fusion protein of Helicoverpa armigera nucleopolyhedrovirus in an early fusion intermediate state
Authors: Chong T / Rao G / Fu Y / Cao S
History
DepositionJul 31, 2025-
Header (metadata) releaseAug 5, 2026-
Map releaseAug 5, 2026-
UpdateAug 5, 2026-
Current statusAug 5, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_65626.png

Downloads & links

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Map

FileDownload / File: emd_65626.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.95 Å/pix.
x 256 pix.
= 243.2 Å		0.95 Å/pix.
x 256 pix.
= 243.2 Å		0.95 Å/pix.
x 256 pix.
= 243.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.95 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.47
Minimum - Maximum-4.0495863 - 6.4498544
Average (Standard dev.)-0.0011001221 (±0.11399015)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 243.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_65626_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_65626_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Membrane fusion protein of Helicoverpa armigera nucleopolyhedrovirus

EntireName: Membrane fusion protein of Helicoverpa armigera nucleopolyhedrovirus
Components
  • Complex: Membrane fusion protein of Helicoverpa armigera nucleopolyhedrovirus
    • Protein or peptide: fusion protein
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose

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Supramolecule #1: Membrane fusion protein of Helicoverpa armigera nucleopolyhedrovirus

SupramoleculeName: Membrane fusion protein of Helicoverpa armigera nucleopolyhedrovirus
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Helicoverpa armigera nucleopolyhedrovirus

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Macromolecule #1: fusion protein

MacromoleculeName: fusion protein / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Helicoverpa armigera nucleopolyhedrovirus
Molecular weightTheoretical: 71.981109 KDa
Recombinant expressionOrganism: Drosophila melanogaster (fruit fly)
SequenceString: MKLCILLAVV AFVGLSLGRS MTESFAIDPT NLLKIISLPR TIGLYFDKIY QMRFVEDVWH FVIEVDHNII FEELVQLHNS YNNLVEWIK NKNLTSCENS ETIFHDLQTY IYRQIIELIE KNNAIDHSAV AMNPDLVQID ANRKWPVPLV TDKPTFSSRN K RNIGLNFV ...String:
MKLCILLAVV AFVGLSLGRS MTESFAIDPT NLLKIISLPR TIGLYFDKIY QMRFVEDVWH FVIEVDHNII FEELVQLHNS YNNLVEWIK NKNLTSCENS ETIFHDLQTY IYRQIIELIE KNNAIDHSAV AMNPDLVQID ANRKWPVPLV TDKPTFSSRN K RNIGLNFV GSVDKFLFGV MDADDAEELH ALAKNNNALN EQVKELDDEL IRLVNYEDHL ACIEKMKSET CDYVTNKMKL MQ TQLNELK FLYINLDRAV DSAKYNRLSP TIMTPQRLYN EMRNVTGKLP DSLTWPIELN VNNMHALIDY VVNTHVFVTP QRT LLFIIE VPLVNTENYD LYNIVPVPMC NNNRTNCAVI IPTSKYIGMS QDKRNYVRLD DTHSCKSAGA NTKLCYKPET ILLS NLASL CDIKIFLNEA RDMDLMNDCD VRIGRFDKEI FHPIADFNRW LYMVDEVTEL NFLCKNVKRS LQLDAGVGLL EGIGN KYCK VSTKRSTITL HEVKNNLLIT EYVDMSSTFN FSRAIRDIDR MQFELEALGS NNDLDHDTLK SMTYRLTELR KEINNN TIF KGWQIENDNK PFVGWFSGWN LSTGWQNFLS SSRGPFEGKP IPNPLLGLDS TRTGHHHHHH

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Macromolecule #2: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 2 / Number of copies: 2 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 5.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeJEOL CRYO ARM 300
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 220934
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

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