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- EMDB-65493: in situ structure of mtHsp60-Hsp10 half-football conformation -

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Basic information

Entry
Database: EMDB / ID: EMD-65493
Titlein situ structure of mtHsp60-Hsp10 half-football conformation
Map data
Sample
  • Cell: Human mitochondrial mtHsp60-Hsp10 chaperone complex
Keywordsmthsp60-hsp10 / CHAPERONE
Biological speciesHomo sapiens (human)
Methodsubtomogram averaging / cryo EM / Resolution: 27.0 Å
AuthorsJung M / Roh S
Funding support Korea, Republic Of, 5 items
OrganizationGrant numberCountry
National Research Foundation (NRF, Korea)2019R1C1C1004598 Korea, Republic Of
National Research Foundation (NRF, Korea)2020R1A5A1018081 Korea, Republic Of
National Research Foundation (NRF, Korea)2021M3A9I4021220 Korea, Republic Of
National Research Foundation (NRF, Korea)2019M3E5D6063871 Korea, Republic Of
National Research Foundation (NRF, Korea)2020R1A6C101A183 Korea, Republic Of
CitationJournal: To Be Published
Title: In situ characterization of mtHsp60-Hsp10 chaperone complex under folding stress
Authors: Jung M / Roh S
History
DepositionJul 24, 2025-
Header (metadata) releaseOct 1, 2025-
Map releaseOct 1, 2025-
UpdateOct 15, 2025-
Current statusOct 15, 2025Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_65493.png

Downloads & links

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Map

FileDownload / File: emd_65493.map.gz / Format: CCP4 / Size: 6.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.47 Å/pix.
x 120 pix.
= 295.8 Å		2.47 Å/pix.
x 120 pix.
= 295.8 Å		2.47 Å/pix.
x 120 pix.
= 295.8 Å

Surface

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Slices (1/3)

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Images are generated by Spider.

Voxel sizeX=Y=Z: 2.465 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.484
Minimum - Maximum-0.027069176 - 0.8865079
Average (Standard dev.)0.035204537 (±0.12316419)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions120120120
Spacing120120120
CellA=B=C: 295.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_65493_msk_1.map
Projections & Slices
AxesZYX

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Half map: #1

Fileemd_65493_half_map_1.map
Projections & Slices
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Histogram

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Half map: #2

Fileemd_65493_half_map_2.map
Projections & Slices
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Sample components

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Entire : Human mitochondrial mtHsp60-Hsp10 chaperone complex

EntireName: Human mitochondrial mtHsp60-Hsp10 chaperone complex
Components
  • Cell: Human mitochondrial mtHsp60-Hsp10 chaperone complex

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Supramolecule #1: Human mitochondrial mtHsp60-Hsp10 chaperone complex

SupramoleculeName: Human mitochondrial mtHsp60-Hsp10 chaperone complex / type: cell / ID: 1 / Parent: 0
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statecell

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Sample preparation

BufferpH: 7
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 3.2 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 3.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

DetailsFalcon4i
Final reconstructionApplied symmetry - Point group: C7 (7 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 27.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 4.0) / Number subtomograms used: 976
ExtractionNumber tomograms: 461 / Number images used: 107626 / Software - Name: Warp
CTF correctionType: NONE
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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