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- EMDB-65377: The Non-uniform refinement map(no sharp) of ITN bound OXGR1 -

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Basic information

Entry
Database: EMDB / ID: EMD-65377
TitleThe Non-uniform refinement map(no sharp) of ITN bound OXGR1
Map data
Sample
  • Complex: ITN bound OXGR1 Non-uniform refinement map(no sharp)
KeywordsGPCR / MEMBRANE PROTEIN
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.62 Å
AuthorsSu Q / Chen ZY / Liang EY / Tang XF / Ma TS
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: To Be Published
Title: The Non-uniform refinement map(no sharp) of ITN bound OXGR1
Authors: Su Q / Chen ZY / Liang EY / Tang XF / Ma TS
History
DepositionJul 14, 2025-
Header (metadata) releaseJul 8, 2026-
Map releaseJul 8, 2026-
UpdateJul 8, 2026-
Current statusJul 8, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_65377.png

Downloads & links

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Map

FileReleased
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.94 Å/pix.
x 324 pix.
= 303.264 Å		0.94 Å/pix.
x 324 pix.
= 303.264 Å		0.94 Å/pix.
x 324 pix.
= 303.264 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.936 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.143
Minimum - Maximum-0.39036724 - 0.81497407
Average (Standard dev.)-0.00026853176 (±0.016648185)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions324324324
Spacing324324324
CellA=B=C: 303.264 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_65377_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_65377_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : ITN bound OXGR1 Non-uniform refinement map(no sharp)

EntireName: ITN bound OXGR1 Non-uniform refinement map(no sharp)
Components
  • Complex: ITN bound OXGR1 Non-uniform refinement map(no sharp)

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Supramolecule #1: ITN bound OXGR1 Non-uniform refinement map(no sharp)

SupramoleculeName: ITN bound OXGR1 Non-uniform refinement map(no sharp) / type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: NITROGEN

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Electron microscopy

MicroscopeFEI POLARA 300
Image recordingFilm or detector model: FEI FALCON I (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.62 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: Coot / Number images used: 334276
Initial angle assignmentType: OTHER
Final angle assignmentType: OTHER

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