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- EMDB-65330: cryoEM structure of tobacco mosaic virus tRNA-like structure comp... -

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Basic information

Entry
Database: EMDB / ID: EMD-65330
TitlecryoEM structure of tobacco mosaic virus tRNA-like structure complexed with tobacco 60S and cycloheximide (CHX)
Map data
Sample
  • Complex: tobacco 80S ribosome with tRNA-like structure
    • Protein or peptide: x 42 types
    • RNA: x 4 types
KeywordstRNA-like structure / ribosome / tobacco / Nicotiana benthamiana / tobacco mosaic virus / RNA structure / translation
Function / homology
Function and homology information


adaxial/abaxial pattern specification / leaf morphogenesis / response to high light intensity / response to UV-B / protein-RNA complex assembly / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly ...adaxial/abaxial pattern specification / leaf morphogenesis / response to high light intensity / response to UV-B / protein-RNA complex assembly / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / cell division / mRNA binding / RNA binding / zinc ion binding / nucleus / cytoplasm
Similarity search - Function
Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ribosomal protein L28e / : / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L23 / Ribosomal protein L2, archaeal-type / Ribosomal protein L1, conserved site / Ribosomal protein L1 signature. ...Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ribosomal protein L28e / : / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L23 / Ribosomal protein L2, archaeal-type / Ribosomal protein L1, conserved site / Ribosomal protein L1 signature. / Ribosomal protein L1 / metallochaperone-like domain / TRASH domain / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L19, eukaryotic / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L44e signature. / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein L10e / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein L30e signature 1. / Ribosomal L40e family / Ribosomal protein L36e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / Eukaryotic Ribosomal Protein L27, KOW domain / : / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / : / Ribosomal_L40e / Ribosomal protein L27e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L44e / Ribosomal Protein L6, KOW domain / Ribosomal protein L44 / Ribosomal protein 60S L18 and 50S L18e / 60S ribosomal protein L35 / Ribosomal protein L30e signature 2. / Ribosomal protein L30e, conserved site / Ribosomal protein L6e / : / Ribosomal protein L7A/L8 / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / Ribosomal protein L34Ae / Ribosomal protein L34e / 60S ribosomal protein L6E / Ribosomal protein L7, eukaryotic / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / Ribosomal protein L30/YlxQ / 60S ribosomal protein L19 / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L31e, conserved site / : / Ribosomal protein L31e signature. / Ribosomal protein L36e / Ribosomal protein L14e domain / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L14 / Ribosomal protein L18e / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L35A superfamily / : / : / Ribosomal protein L19e, C-terminal domain
Similarity search - Domain/homology
60s ribosomal protein l27a-3 / 60S ribosomal protein L35-like / 60S ribosomal protein L30 / 60S ribosomal protein L11-1 / 60S ribosomal protein L27 / 60S ribosomal protein L21-1-like / 60S ribosomal protein L5-like / Ribosomal protein L15 / 60S ribosomal protein L23 / 60S ribosomal protein L8-like ...60s ribosomal protein l27a-3 / 60S ribosomal protein L35-like / 60S ribosomal protein L30 / 60S ribosomal protein L11-1 / 60S ribosomal protein L27 / 60S ribosomal protein L21-1-like / 60S ribosomal protein L5-like / Ribosomal protein L15 / 60S ribosomal protein L23 / 60S ribosomal protein L8-like / Ribosomal protein / 60S ribosomal protein L31 / 60S ribosomal protein L10 / Large ribosomal subunit protein uL13w / 60S ribosomal protein L3 / Ribosomal protein L19 / Large ribosomal subunit protein eL33y-like / 60S ribosomal protein L24 / Large ribosomal subunit protein eL22 / Large ribosomal subunit protein uL4-like / 60S ribosomal protein L38 / Large ribosomal subunit protein uL24z / Large ribosomal subunit protein uL6z/uL6y / 60S ribosomal protein L13 / 60S ribosomal protein L18-2 / Ribosomal protein L37 / Large ribosomal subunit protein uL22z / Large ribosomal subunit protein eL14z / 60S ribosomal protein L18a / 60S ribosomal protein L36 / Large ribosomal subunit protein eL6-like / Large ribosomal subunit protein eL28z-like / Large ribosomal subunit protein uL30w-like / Ubiquitin-ribosomal protein eL40 fusion protein / 60S ribosomal protein L7a / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein eL39x / Large ribosomal subunit protein eL32z / Large ribosomal subunit protein eL42 / Large ribosomal subunit protein eL34 / Large ribosomal subunit protein uL23
Similarity search - Component
Biological speciesNicotiana benthamiana (plant) / synthetic construct (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.5 Å
AuthorsLu G / Lin J
Funding support China, 2 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32130063 China
National Natural Science Foundation of China (NSFC)32471349 China
CitationJournal: To be published
Title: cryoEM structure of tobacco mosaic virus tRNA-like structure complexed with tobacco 60S and cycloheximide (CHX)
Authors: Lu G / Lin J
History
DepositionJul 10, 2025-
Header (metadata) releaseJul 15, 2026-
Map releaseJul 15, 2026-
UpdateJul 15, 2026-
Current statusJul 15, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_65330.png

Downloads & links

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Map

FileDownload / File: emd_65330.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesX (Sec.)Y (Row.)Z (Col.)
0.96 Å/pix.
x 480 pix.
= 460.32 Å		0.96 Å/pix.
x 480 pix.
= 460.32 Å		0.96 Å/pix.
x 480 pix.
= 460.32 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.959 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-0.60669655 - 1.8166472
Average (Standard dev.)0.000023305201 (±0.047875755)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 460.32 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : tobacco 80S ribosome with tRNA-like structure

EntireName: tobacco 80S ribosome with tRNA-like structure
Components
  • Complex: tobacco 80S ribosome with tRNA-like structure
    • Protein or peptide: Ribosomal protein
    • Protein or peptide: 60S ribosomal protein L8-like
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L4-like
    • Protein or peptide: 60S ribosomal protein L11-1
    • Protein or peptide: 60S ribosomal protein L9-1
    • Protein or peptide: 60S ribosomal protein L13a-4
    • Protein or peptide: 60S ribosomal protein L23
    • Protein or peptide: 60s ribosomal protein l27a-3
    • Protein or peptide: 60S ribosomal protein L10
    • Protein or peptide: 60S ribosomal protein L5-like
    • Protein or peptide: 60S ribosomal protein L17-1 isoform X2
    • Protein or peptide: Large ribosomal subunit protein uL23
    • Protein or peptide: 60S ribosomal protein L26-1
    • Protein or peptide: 60S ribosomal protein L35-like
    • Protein or peptide: 60S ribosomal protein L7-4-like
    • Protein or peptide: 60S ribosomal protein L6-like isoform X1
    • Protein or peptide: 60S ribosomal protein L7a
    • Protein or peptide: 60S ribosomal protein L13
    • Protein or peptide: 60S ribosomal protein L14-1
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: 60S ribosomal protein L18-2
    • Protein or peptide: Ribosomal protein L19
    • Protein or peptide: 60S ribosomal protein L18a
    • Protein or peptide: 60S ribosomal protein L21-1-like
    • Protein or peptide: Large ribosomal subunit protein eL22
    • Protein or peptide: 60S ribosomal protein L24
    • Protein or peptide: 60S ribosomal protein L27
    • Protein or peptide: 60S ribosomal protein L28-1-like
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L30
    • Protein or peptide: 60S ribosomal protein L31
    • Protein or peptide: 60S ribosomal protein L32-1
    • Protein or peptide: 60S ribosomal protein L35a-3-like
    • Protein or peptide: Large ribosomal subunit protein eL34
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: 60S ribosomal protein L38
    • Protein or peptide: 60S ribosomal protein L39-3
    • Protein or peptide: Ubiquitin-60S ribosomal protein L40
    • Protein or peptide: 60S ribosomal protein L44
    • Protein or peptide: 60S ribosomal protein L37a
    • RNA: RNA (204-MER)
    • RNA: 5S rRNA
    • RNA: 5.8S rRNA
    • RNA: 25S rRNA

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Supramolecule #1: tobacco 80S ribosome with tRNA-like structure

SupramoleculeName: tobacco 80S ribosome with tRNA-like structure / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Nicotiana benthamiana (plant)

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Macromolecule #1: Ribosomal protein

MacromoleculeName: Ribosomal protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 24.778387 KDa
SequenceString: MSSKLQSDAL REAISVIKND CAEKKRKFTE TIELQIGLKN YDPQKDKRFS GSVKLPHIPR PKMKICMLGD AQHVEEAEKI GLEYMDVES LKKLNKNKKL VKKLAKKYHA FLASESVIKQ IPRLLGPGLN KAGKFPSLVS HQESLESKVN ETKATIKFQL K KVLCMGVA ...String:
MSSKLQSDAL REAISVIKND CAEKKRKFTE TIELQIGLKN YDPQKDKRFS GSVKLPHIPR PKMKICMLGD AQHVEEAEKI GLEYMDVES LKKLNKNKKL VKKLAKKYHA FLASESVIKQ IPRLLGPGLN KAGKFPSLVS HQESLESKVN ETKATIKFQL K KVLCMGVA VGNLDMEEKQ IFQNVQMSVN FLVSLLKKNW QNVRCLYLKS TMGKPQRIF

UniProtKB: Ribosomal protein

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Macromolecule #2: 60S ribosomal protein L8-like

MacromoleculeName: 60S ribosomal protein L8-like / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 28.220734 KDa
SequenceString: MGRVIRAQRK GAGSVFKSHT HHRKGPAKFR SLDYGERNGY LKGVVSEIIH DPGRGAPLAR VTFRHPFRYK HQKELFVAAE GMYTGQFIY CGKKANLMVG NVLALRSIPE GAVVCNVEHK VGDRGVFARC SGDYAIVISH NPDNGTTRIK LPSGAKKIVP S GCRAMIGQ ...String:
MGRVIRAQRK GAGSVFKSHT HHRKGPAKFR SLDYGERNGY LKGVVSEIIH DPGRGAPLAR VTFRHPFRYK HQKELFVAAE GMYTGQFIY CGKKANLMVG NVLALRSIPE GAVVCNVEHK VGDRGVFARC SGDYAIVISH NPDNGTTRIK LPSGAKKIVP S GCRAMIGQ VAGGGRTEKP MLKAGNAYHK YRVKRNCWPK VRGVAMNPVE HPHGGGNHQH IGHASTVRRD APPGQKVGLI AA RRTGRLR GQAAATAAKA DKA

UniProtKB: 60S ribosomal protein L8-like

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Macromolecule #3: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 44.552254 KDa
SequenceString: MSHRKFEHPR HGSLGFLPRK RAARHRGKVK AFPKDDPSKP CKLTAFLGYK AGMTHIVRDV EKPGSKLHKK ETCEAVTIIE TPPMVIVGV VGYVKTPRGL RCLNTVWAQH LSEELKRRFY KNWCKSKKKA FLKYSKKYET DEGKKDIQTQ LEKLKKYACV I RVLAHTQI ...String:
MSHRKFEHPR HGSLGFLPRK RAARHRGKVK AFPKDDPSKP CKLTAFLGYK AGMTHIVRDV EKPGSKLHKK ETCEAVTIIE TPPMVIVGV VGYVKTPRGL RCLNTVWAQH LSEELKRRFY KNWCKSKKKA FLKYSKKYET DEGKKDIQTQ LEKLKKYACV I RVLAHTQI RKMKGLKQKK AHLMEIQVNG GTIAQKVDFA YSFFEKQVPV DAVFQKDEMI DIIGVTKGKG YEGVVTRWGV TR LPRKTHR GLRKVACIGA WHPARVSYTV ARAGQNGYHH RTEMNKKVYK LGKAGQESHA AITDFDRTEK EITPMGGFPH YGV VKDDYL LIKGCCVGPK KRVVTLRQSL LNQTSRVALE EIKLKFIDTS SKFGHGRFQT TQEKEKFYGR LKA

UniProtKB: 60S ribosomal protein L3

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Macromolecule #4: 60S ribosomal protein L4-like

MacromoleculeName: 60S ribosomal protein L4-like / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 44.778945 KDa
SequenceString: MATAAAIPTT TVQSFENDMA TDGAVPVPAV MKAPIRPDVV TYVHSNISKN ARQPYAVSKK AGHQTSAESW GTGRAVSRIP RVAGGGTHR AGQAAFGNMC RGGRMFAPTK IWRRWHRKIP VNQKRYAVAS AIAASAVPSL VLARGHRIES VPELPLVVSD S VESIEKTS ...String:
MATAAAIPTT TVQSFENDMA TDGAVPVPAV MKAPIRPDVV TYVHSNISKN ARQPYAVSKK AGHQTSAESW GTGRAVSRIP RVAGGGTHR AGQAAFGNMC RGGRMFAPTK IWRRWHRKIP VNQKRYAVAS AIAASAVPSL VLARGHRIES VPELPLVVSD S VESIEKTS NAVKALKQIG AYPDAEKAKD SHAIRPGKGK MRNRRYISRK GPLIVYGTEG AKLVKAFRNI PGVEICHVDR LN LLKLAPG GHLGRFIVWT KSAYEKLDEI YGSFDKASEK KKGYVLPRPK MVNADLARII NSDEVQSVVR PIKKDVNKRA TLK KNPLKN LNVLLKLNPY AKTARRMSLL AEAQRVKAKK EKLDKKRHQV TKEEASAIRA AGTSWFKTMI SDSDYTEFEN FTKW LGVTQ

UniProtKB: Large ribosomal subunit protein uL4-like

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Macromolecule #5: 60S ribosomal protein L11-1

MacromoleculeName: 60S ribosomal protein L11-1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 20.794178 KDa
SequenceString:
MASEKKLSNP MREIKVQKLV LNISVGESGD RLTRAAKVLE QLSGQSPVFS KARYTVRSFG IRRNEKIACY VTVRGEKAMQ LLESGLKVK EYELLRRNFS ETGCFGFGIQ EHIDLGIKYD PSTGIYGMDF YVVLERPGYR VARRRRCKAR VGIQHRVTKD D AMKWFQVK YEGVILNKSS NIQ

UniProtKB: 60S ribosomal protein L11-1

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Macromolecule #6: 60S ribosomal protein L9-1

MacromoleculeName: 60S ribosomal protein L9-1 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 22.058736 KDa
SequenceString: MKTILSSETM DIPDGVKIKV KAKQIEVEGP RGKLTRNFKH LNLDFQLIKD EETGKKKLKI DAWFGSRKTT AAIRTALSHV ENLITGVTK GYRYKMRFVY AHFPINASIT GGNKAIEIRN FLGEKKVRKV DMLEGVTVVR SEKVKDELVL DGNDIELVSR S AALINQKC ...String:
MKTILSSETM DIPDGVKIKV KAKQIEVEGP RGKLTRNFKH LNLDFQLIKD EETGKKKLKI DAWFGSRKTT AAIRTALSHV ENLITGVTK GYRYKMRFVY AHFPINASIT GGNKAIEIRN FLGEKKVRKV DMLEGVTVVR SEKVKDELVL DGNDIELVSR S AALINQKC HVKNKDIRKF LDGIYVSEKG RIVEEE

UniProtKB: Large ribosomal subunit protein uL6z/uL6y

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Macromolecule #7: 60S ribosomal protein L13a-4

MacromoleculeName: 60S ribosomal protein L13a-4 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 23.583211 KDa
SequenceString: MVSGSGISAR RVVVDARHHM LGRLSSILAK ELLNGQRVVV VRCEEICLSG GLVRQKMKYL RFLRKRMNTK PSHGPIHFRA PSKILWRTI RGMIPHKTKR GAAALARLKV YEGVPPPYDK IKRMVIPDAL KVLRLQAGHK YCLLGKLSSE VGWNHYDTIK E LENKRKER ...String:
MVSGSGISAR RVVVDARHHM LGRLSSILAK ELLNGQRVVV VRCEEICLSG GLVRQKMKYL RFLRKRMNTK PSHGPIHFRA PSKILWRTI RGMIPHKTKR GAAALARLKV YEGVPPPYDK IKRMVIPDAL KVLRLQAGHK YCLLGKLSSE VGWNHYDTIK E LENKRKER AQVAYERRKQ LAKLRVKAEK AAEEKLGPQL AVIEPIKY

UniProtKB: Large ribosomal subunit protein uL13w

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Macromolecule #8: 60S ribosomal protein L23

MacromoleculeName: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 15.040858 KDa
SequenceString:
MSKRGRGGSA GNKFRMSLGL PVAATINCAD NTGAKNLYII SVKGIKGRLN RLPSACVGDM VMATVKKGKP DLRKKVMPAV VVRQRKPWR RKDGVFMYFE DNAGVIVNPK GEMKGSAITG PIGKECADLW PRIASAANAI V

UniProtKB: 60S ribosomal protein L23

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Macromolecule #9: 60s ribosomal protein l27a-3

MacromoleculeName: 60s ribosomal protein l27a-3 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 16.504334 KDa
SequenceString:
MTTRFKKNRK KRGHVSAGHG RVGKHRKHPG GRGNAGGMHH HRILFDKYHP GYFGKVGMRY FHKLRNKFHC PTINIDNLWS LVPQEVKDK AKSSSGTAPL IDVTQYGYFK VLGKGVLPEN QAVVVKAKLI SKNAEKKIKE AGGAVVLTA

UniProtKB: 60s ribosomal protein l27a-3

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Macromolecule #10: 60S ribosomal protein L10

MacromoleculeName: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 24.841266 KDa
SequenceString: MGRRPARCYR QIKNKPYPKS RFCRGVPDPK IRIYDVGMKK KGVDEFPFCV HLVSWEKENV SSEALEAARI ACNKYMTKSA GKDAFHLRV RVHPFHVLRI NKMLSCAGAD RLQTGMRGAF GKPQGVCARV AIGQVLLSVR CKDGNANHAQ EALRRAKFKF P GRQKIIVS ...String:
MGRRPARCYR QIKNKPYPKS RFCRGVPDPK IRIYDVGMKK KGVDEFPFCV HLVSWEKENV SSEALEAARI ACNKYMTKSA GKDAFHLRV RVHPFHVLRI NKMLSCAGAD RLQTGMRGAF GKPQGVCARV AIGQVLLSVR CKDGNANHAQ EALRRAKFKF P GRQKIIVS RKWGFTKFSR TDYLKYKSEN RIIPDGVNAK LLGCHGRLAA RQPGRAFLEA V

UniProtKB: 60S ribosomal protein L10

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Macromolecule #11: 60S ribosomal protein L5-like

MacromoleculeName: 60S ribosomal protein L5-like / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 33.69159 KDa
SequenceString: MAFIKVQKTR AYFKRFQVKF KRRREGKTDY RARNRLINQD KNKYNTPKYR FVVRFTNKDI IAQIVSASIA GDMILASAYA RELPRYGLK VGLTNYAAAY CTGLLLARRV LKKLEMDEEY QGNPEATGED YSVEPAESRR PFRALLDVGL VRTTTGNRVF G ALKGALDG ...String:
MAFIKVQKTR AYFKRFQVKF KRRREGKTDY RARNRLINQD KNKYNTPKYR FVVRFTNKDI IAQIVSASIA GDMILASAYA RELPRYGLK VGLTNYAAAY CTGLLLARRV LKKLEMDEEY QGNPEATGED YSVEPAESRR PFRALLDVGL VRTTTGNRVF G ALKGALDG GLDIPHSEKR FAGFSKDSKQ LDADVHRKYI YGGHVATYMK TLTEDEPEKF QSHFSEYIKQ GLEADDLEEM YK KVHAAIR ADPSPKKSEK QPPKQHKRYN LKKLTYEERK AKLIERLNAL NSAAGN

UniProtKB: 60S ribosomal protein L5-like

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Macromolecule #12: 60S ribosomal protein L17-1 isoform X2

MacromoleculeName: 60S ribosomal protein L17-1 isoform X2 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 19.906934 KDa
SequenceString:
MVKYSREPDN PTKSCKARGA SLRVHFKNTR ETAHALRKMP LDKAKKYLED VLAHKQAIPF TRFCGGVGRT AQVKHRHSNG QGRWPVKSA KIILDLLKNA ESNAEVKGLD VDSLFISHIQ VNQAQKQRRR TYRAHGRINP YMSHPCHVEL TLSEKEESVK K EPETQLAS RKAKASS

UniProtKB: Large ribosomal subunit protein uL22z

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Macromolecule #13: Large ribosomal subunit protein uL23

MacromoleculeName: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 17.355531 KDa
SequenceString:
MAPAKADPSK KSDPKAQAAK VAKAVKSGST FKKKSQKIRT KVTFHRPKTL KKDRNPKYPR ISAPGRNKLD QYGILKYPLT TESAMKKIE DNNTLVFIVD IKADKKKIKD AVKKMYDIQT KKVNTLIRPD GTKKAYVRLT PDYDALDVAN KIGII

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #14: 60S ribosomal protein L26-1

MacromoleculeName: 60S ribosomal protein L26-1 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 16.808588 KDa
SequenceString:
MKYNPRVSSS RRKSRKAHFT APSSVRRVLM SAPLSSELRT KYNVRSMPVR KDDEVQVVRG TYKGREGKVV QVYRKKWVIH IERITREKV NGSTVNVGIH PSKTVITKLR LDKDRKSLID RKAKGRAAAD KDKGTKFTTE DIMQTVD

UniProtKB: Large ribosomal subunit protein uL24z

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Macromolecule #15: 60S ribosomal protein L35-like

MacromoleculeName: 60S ribosomal protein L35-like / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 14.324413 KDa
SequenceString:
MARIKVHELR NKTKTELLAQ LKDLKAELAL LRVAKVTGGA PNKLSKIKVV RLSIAQVLTV ISQKQKTALR EAYKNKKYLP LDLRPKKTR AIRKRLTKHQ ASLKTEREKK KEMYFPIRKY AIKV

UniProtKB: 60S ribosomal protein L35-like

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Macromolecule #16: 60S ribosomal protein L7-4-like

MacromoleculeName: 60S ribosomal protein L7-4-like / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 28.147945 KDa
SequenceString: MGEGGVPVPE SVLKKQKRSE EWALVKKQEL EAAKKKNTEN RKLIYNRAKQ YAKEYEAQER ELIRLKREAR LKGGFYVDPE AKLLFIIRI RGINAMAPQT KKILQLLRLR QIFNGVFLKV NKATVNMLHR VEPYVTYGYP NLKSIRELIY KRGYGKVDKQ R IALTDNSV ...String:
MGEGGVPVPE SVLKKQKRSE EWALVKKQEL EAAKKKNTEN RKLIYNRAKQ YAKEYEAQER ELIRLKREAR LKGGFYVDPE AKLLFIIRI RGINAMAPQT KKILQLLRLR QIFNGVFLKV NKATVNMLHR VEPYVTYGYP NLKSIRELIY KRGYGKVDKQ R IALTDNSV IEQVLGKHGI ICIEDLVHEI ITVGPHFKEA NNFLWPFQLK APLGGLKKKR NHYVEGGDAG NRENYINELI RR MN

UniProtKB: Large ribosomal subunit protein uL30w-like

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Macromolecule #17: 60S ribosomal protein L6-like isoform X1

MacromoleculeName: 60S ribosomal protein L6-like isoform X1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 25.493291 KDa
SequenceString: MAAKKSPRNP ELIRGVGKLS RSKMYHKKGL WAIKKKNGGS FPVHKKAAAV APPAVKPPKI YPADDVAKPL VNKHKPKPTK LRASITPGT VLIILAGKFK GKRVVFLKQL KSGLLLVSGP FKLNGVPLRR VNQAYVIGTS TKVDVSGVSV EKFDDKYFAK Q AEKKQKKG ...String:
MAAKKSPRNP ELIRGVGKLS RSKMYHKKGL WAIKKKNGGS FPVHKKAAAV APPAVKPPKI YPADDVAKPL VNKHKPKPTK LRASITPGT VLIILAGKFK GKRVVFLKQL KSGLLLVSGP FKLNGVPLRR VNQAYVIGTS TKVDVSGVSV EKFDDKYFAK Q AEKKQKKG EGEFFEDKKE EKKNVLPQEK KDDQKAVDEA LIKAVECVPE LKAYLSARFS LKSGMKPHEL VF

UniProtKB: Large ribosomal subunit protein eL6-like

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Macromolecule #18: 60S ribosomal protein L7a

MacromoleculeName: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 27.223457 KDa
SequenceString: NPLFEKRPKQ FGIGGALPPK KDLTRFVKWP QVVRIQRKRR ILKQRLKVPP ALNQFSKTLD KNLATNLFKM LLKYRPEDKA AKKERLIKK AQAEAEGKTP ETKKPIIVKY GLKHITYLIE QNKAQLVVIA HDVDPIELVV WLPALCRKME IPYCIVKGKA R LGSIVHKK ...String:
NPLFEKRPKQ FGIGGALPPK KDLTRFVKWP QVVRIQRKRR ILKQRLKVPP ALNQFSKTLD KNLATNLFKM LLKYRPEDKA AKKERLIKK AQAEAEGKTP ETKKPIIVKY GLKHITYLIE QNKAQLVVIA HDVDPIELVV WLPALCRKME IPYCIVKGKA R LGSIVHKK TASALCLTTV KNEDKMEFSR ILEAIKANFN DKYEENRKKW GGGVMGSKSQ ARTKAKERVL AKEAAQRMN

UniProtKB: 60S ribosomal protein L7a

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Macromolecule #19: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 22.862719 KDa
SequenceString: MKHNNVIPNG HFKKHWQNYV RTWFNQPARK TRRRAARQQK AVKIFPRPTA GSLRPIVHGQ TLKYNMKVRA GRGFSLEELK AAGIPKKLA PTIGIAVDHR RRNRSLEGLQ TNVQRLKTYK AKLVIFPRRA KKVKAGDSSV EELATATQVQ GSYMPITREQ P AVELVKVT ...String:
MKHNNVIPNG HFKKHWQNYV RTWFNQPARK TRRRAARQQK AVKIFPRPTA GSLRPIVHGQ TLKYNMKVRA GRGFSLEELK AAGIPKKLA PTIGIAVDHR RRNRSLEGLQ TNVQRLKTYK AKLVIFPRRA KKVKAGDSSV EELATATQVQ GSYMPITREQ P AVELVKVT DEMKSFNAYG KLRIERTNAR HIGARLKRAA EA

UniProtKB: 60S ribosomal protein L13

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Macromolecule #20: 60S ribosomal protein L14-1

MacromoleculeName: 60S ribosomal protein L14-1 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 15.027004 KDa
SequenceString:
MPFKRFVEIG RVALVNYGKD YGKLVVIVDV IDQNRALVDA PDMVRSQINF KRLSLTDIKI DIKRIPKKKT LVAAMEAADV KTKWENSSW GRKLIVQKRR AALNDFDRFK LMLAKIKRAG VVRQELAKLK K

UniProtKB: Large ribosomal subunit protein eL14z

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Macromolecule #21: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 24.226291 KDa
SequenceString: MGAYTYVSEL WRKKQSDVMR FLQRVRCWEY RQLPSIVRVT RPTRPDKARR LGYKAKQGYV VYRVRVKRGG RKRPVPKGIV YGKPTNQGV TQLKFQRSKR SVAEERAGRK LAGLKVLNSY WINEDSTYKY FEVILVDAAH AAIRNDPRIN WICNPVHKHR E LRGLTSAG ...String:
MGAYTYVSEL WRKKQSDVMR FLQRVRCWEY RQLPSIVRVT RPTRPDKARR LGYKAKQGYV VYRVRVKRGG RKRPVPKGIV YGKPTNQGV TQLKFQRSKR SVAEERAGRK LAGLKVLNSY WINEDSTYKY FEVILVDAAH AAIRNDPRIN WICNPVHKHR E LRGLTSAG KKYRGLRGKG HLYHKNRPSR RGTWKRNQTL SLRRYR

UniProtKB: Ribosomal protein L15

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Macromolecule #22: 60S ribosomal protein L18-2

MacromoleculeName: 60S ribosomal protein L18-2 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 20.905824 KDa
SequenceString:
MGIDLKAGGK SKKTKRTAPK SDDVYLKLLV KLYRFLVRRT GSKFNAVILK RLFMSKINKP PLSLSRLISY AKGKEDKVVV IVGTITDDV RAYEVPALKV CALRFTKTAR ARIEKAGGEC LTFDQLALRA PLGQNTLLLR GPKNAREAVK HFGPAPGVPH S HTKPFVRS KGRKFERARG RRNSRGYKA

UniProtKB: 60S ribosomal protein L18-2

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Macromolecule #23: Ribosomal protein L19

MacromoleculeName: Ribosomal protein L19 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 24.742324 KDa
SequenceString: MVSLKLQKRL AASVLKCGRG KVWLDPNEGN EISMANSRQN IRKLVKDGFI IRKPTKIHSR SRARRMKEAK RKGRHSGYGK RKGTREARL PTKVLWMRRM RVLRRLLRKY RESKKIDRHM YHDMYMKVKG NVFKNKRVLM ENIHKTKAEK AREKTLSDQF E ARRAKNKA ...String:
MVSLKLQKRL AASVLKCGRG KVWLDPNEGN EISMANSRQN IRKLVKDGFI IRKPTKIHSR SRARRMKEAK RKGRHSGYGK RKGTREARL PTKVLWMRRM RVLRRLLRKY RESKKIDRHM YHDMYMKVKG NVFKNKRVLM ENIHKTKAEK AREKTLSDQF E ARRAKNKA SRERKFARRE ERLAQGPGER PVQPAASATP AQTAQGGSKK SKK

UniProtKB: Ribosomal protein L19

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Macromolecule #24: 60S ribosomal protein L18a

MacromoleculeName: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 21.513348 KDa
SequenceString:
MVTYKFHQYQ VVGRALPTEN DEHPKIYRMK LWATNEVRAK SKFWYYLRKL KKVKKSNGQM LAINEIFEKN PTKIKNYGIW LRYQSRTGY HNMYKEYRDT TLNGAVEQMY TEMASRHRVR HHCIQIIKTA TIPAKLCKRE STKQFHDSKI KFPLVFKKVR P PSRKLKTT YKATRPNLFM

UniProtKB: 60S ribosomal protein L18a

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Macromolecule #25: 60S ribosomal protein L21-1-like

MacromoleculeName: 60S ribosomal protein L21-1-like / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 18.716885 KDa
SequenceString:
MPAGHGLRSR TRDSFSRAFR KKGTIPLSTY LRIFKIGDYV DIKVNGAIHK GMPHKFYHGR TGRVWNVTKR AVGVEVNKQV RNKILRKRI HVRIEHVQLS RCTEEVKERM KKNDQLKAEA KARGEVISTK RQPVGPKPGF MVEGATLETV TPIPYDVVND L KGGY

UniProtKB: 60S ribosomal protein L21-1-like

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Macromolecule #26: Large ribosomal subunit protein eL22

MacromoleculeName: Large ribosomal subunit protein eL22 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 12.50627 KDa
SequenceString:
TFVIDCSKPV EDKIMEIASL EKFLQERIKV GGKAGALGDS VAVARDKTKI TVTSDTTFSK RYLKYLTKKY LKKNNVRDWL RVISSNKDR NVYELRYFNI AENEAEEED

UniProtKB: Large ribosomal subunit protein eL22

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Macromolecule #27: 60S ribosomal protein L24

MacromoleculeName: 60S ribosomal protein L24 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 18.606016 KDa
SequenceString:
MVLKTELCRF SGGKIYPGRG IRFIRSDSQV FLFLNSKCKR YFHNRLKPSK LTWTAMYRKQ HKKDIAQEAV KKRRRATKKP YSRSIVGAT LEVIQKKRTE RPEVRDAARE AALREIKERI KKTKDEKKAK KAEVQAKSQK AGGKGNMPKG AGGKGPKLGG G GGKR

UniProtKB: 60S ribosomal protein L24

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Macromolecule #28: 60S ribosomal protein L27

MacromoleculeName: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 15.692682 KDa
SequenceString:
MVKFLKPNKA VIILQGKYAG RKAVIIRAFD EGTRDRPYGH CLVAGISKYP KKVIRKDSAK KQAKKSRVKA FIKLVNYNHI MPTRYTLDV DLKDVVNVDV LQSRDKKVTA AKEAKARLEE RFKTGKNRWF FTKLRF

UniProtKB: 60S ribosomal protein L27

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Macromolecule #29: 60S ribosomal protein L28-1-like

MacromoleculeName: 60S ribosomal protein L28-1-like / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 15.987686 KDa
SequenceString:
MTTVPGQLVW EIVKKNNSFL VKEFGNGTAG VVFSKEPNNL YNLHSYKHSG LAYKKTVTIQ AEGKDKSVLL ATSKTKKQNK PSTLLNKSV MKKEFRRMAK AVTNQVADNY YRPDLKKAAL ARLSAVNRSL KVAKSGVKKK NRQA

UniProtKB: Large ribosomal subunit protein eL28z-like

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Macromolecule #30: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 5.914666 KDa
SequenceString:
MAKSKNHTSH NQNSKDHRNG IHKAADHYYS STRGMNAKFL RNLRFAKKWN

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Macromolecule #31: 60S ribosomal protein L30

MacromoleculeName: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 12.420518 KDa
SequenceString:
MATTGKKTKK THESINNRLA LVMKSGKYSL GYKTVLKTLR SSKGKLILIS NNCPPLRKSE IEYYAMLAKV GVHHFNGNNV DLGTACGKY FRVSCLSIID PGDSDIIKSL PGDH

UniProtKB: 60S ribosomal protein L30

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Macromolecule #32: 60S ribosomal protein L31

MacromoleculeName: 60S ribosomal protein L31 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 13.894171 KDa
SequenceString:
MSDKTKGRKE EVVTREYTIN LHKRLHSCTF KKKAPTAIKE IRKFAQKAMG TKDVRVDVKL NKQIWSRGIR SVPRRVRVRI ARKRNDDED AKEELYSLVT VAEIPAEGLK GLGTKIIEDE D

UniProtKB: 60S ribosomal protein L31

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Macromolecule #33: 60S ribosomal protein L32-1

MacromoleculeName: 60S ribosomal protein L32-1 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 15.637452 KDa
SequenceString:
MAVPLLTKTV VKKRVKRFIR PQSDRRITVK ESWRRPKGID SRVRRKFKGC VLMPNIGYGS DKKTRHYLPN GFKKFVVHNA SELEVLMMH NRTYCAEIAH NVSTRKRKEI VERAAQLDVV ITNKLARLRS QEDE

UniProtKB: Large ribosomal subunit protein eL32z

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Macromolecule #34: 60S ribosomal protein L35a-3-like

MacromoleculeName: 60S ribosomal protein L35a-3-like / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 12.959179 KDa
SequenceString:
MVKGRQGERI RLYVRGTVLG YKRSKSNQYP STSLIQIEGV NTKEEVDWYL GKRMAYVYKA KTKKNNSHYR CIWGKVCRPH GNSGVVRAK FKSNLPPKSM GSKVRVFMYP SNI

UniProtKB: Large ribosomal subunit protein eL33y-like

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Macromolecule #35: Large ribosomal subunit protein eL34

MacromoleculeName: Large ribosomal subunit protein eL34 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 13.788358 KDa
SequenceString:
MVQRLTYRKR HSYATKSNQH RVVKTPGGKL IYQSTKKRAS GPKCPVTGKR IQGIPHLRPT EYKRSRLSRN RRTVNRAYGG VLSGSAVRE RIIRAFLVEE QKIVKKVLKI QKAKEKLAAK S

UniProtKB: Large ribosomal subunit protein eL34

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Macromolecule #36: 60S ribosomal protein L36

MacromoleculeName: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 12.389871 KDa
SequenceString:
MAPKQPNTGL SVGLNKGHVV TKKELAPRPS DRKGKTSKRV HFVRSLIREV AGFAPYEKRI TELLKVGKDK RALKVAKRKL GTHKRAKKK REEMSNVLRK MRAAGGGEKK K

UniProtKB: 60S ribosomal protein L36

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Macromolecule #37: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 10.879783 KDa
SequenceString:
MGKGTGSFGK RRNKTHTLCV RCGRRSFHIQ KSRCSACAYP AARLRKYNWS VKALRRKTTG TGRMRYLRNV PRRFKTNFRE GTEATPRKK GAAVAS

UniProtKB: Ribosomal protein L37

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Macromolecule #38: 60S ribosomal protein L38

MacromoleculeName: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 8.101704 KDa
SequenceString:
MPKQIHEIKD FLLTARRKDA RTVKIKKNKD MVKFKVRCSK YLYTLCVSDF EKADKLKQSL PPGLSVQDL

UniProtKB: 60S ribosomal protein L38

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Macromolecule #39: 60S ribosomal protein L39-3

MacromoleculeName: 60S ribosomal protein L39-3 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 6.503839 KDa
SequenceString:
MPSHKTFMIK KKLAKKQRQN RPIPYWIRMR TDNTIRYNAK RRHWRRTKLG F

UniProtKB: Large ribosomal subunit protein eL39x

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Macromolecule #40: Ubiquitin-60S ribosomal protein L40

MacromoleculeName: Ubiquitin-60S ribosomal protein L40 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 14.703358 KDa
SequenceString:
MQIFVKTLTG KTITLEVESS DTIDNVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLADYN IQKESTLHLV LRLRGGIIEP SLMALARKY NQDKMICRKC YARLHPRAVN CRKKKCGHSN QLRPKKKIK

UniProtKB: Ubiquitin-ribosomal protein eL40 fusion protein

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Macromolecule #41: 60S ribosomal protein L44

MacromoleculeName: 60S ribosomal protein L44 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 11.991255 KDa
SequenceString:
MVNVPKTKKT YCKSKEGKKH TLHKVTQYKK GKDSLAAQGK RRYDRKQSGY GGQTKPVFHK KAKTTKKIVL RLQCQGCKHV SQHPIKRCK HFEIGGDKKG KGTSLF

UniProtKB: Large ribosomal subunit protein eL42

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Macromolecule #42: 60S ribosomal protein L37a

MacromoleculeName: 60S ribosomal protein L37a / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 8.624188 KDa
SequenceString:
MTKRTKKTGI VGKYGTRYGA SLRKQIKKME VSQHNKYFCE FCGKYAVKRK AVGIWGCKYC GNVKVGGAYT LNTASDV

UniProtKB: Large ribosomal subunit protein eL43

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Macromolecule #43: RNA (204-MER)

MacromoleculeName: RNA (204-MER) / type: rna / ID: 43 / Number of copies: 1
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 65.633805 KDa
SequenceString: GGUAGUCAAG AUGCAUAAUA AAUAACGGAU UGUGUCCGUA AUCACACGUG GUGCGUACGA UAACGCAUAG UGUUUUUCCC UCCACUUAA AUCGAAGGGU UGUGUCUUGG AUCGCGCGGG UCAAAUGUAU AUGGUUCAUA UACAUCCGCA GGCACGUAAU A AAGCGAGG ...String:
GGUAGUCAAG AUGCAUAAUA AAUAACGGAU UGUGUCCGUA AUCACACGUG GUGCGUACGA UAACGCAUAG UGUUUUUCCC UCCACUUAA AUCGAAGGGU UGUGUCUUGG AUCGCGCGGG UCAAAUGUAU AUGGUUCAUA UACAUCCGCA GGCACGUAAU A AAGCGAGG GGUUCGAAUC CCCCCGUUAC CCCCGGUAGG GGCCCA

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Macromolecule #44: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 44 / Number of copies: 1
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 38.328777 KDa
SequenceString:
GGAUGCGAUC AUACCAGCAC UAACGCACCG GAUCCCAUCA GAACUCCGAA GUUAAGCGUG CUUGGGCGAG AGUAGUACUA GGAUGGGUG ACCCCCUGGG AAGUCCUCGU GUUGCAUCCC

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Macromolecule #45: 5.8S rRNA

MacromoleculeName: 5.8S rRNA / type: rna / ID: 45 / Number of copies: 1
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 52.534219 KDa
SequenceString:
AAACGACUCU CGGCAACGGA UAUCUCGGCU CUCGCAUCGA UGAAGAACGU AGCGAAAUGC GAUACUUGGU GUGAAUUGCA GAAUCCCGU GAACCAUCGA GUCUUUGAAC GCAAGUUGCG CCCGAAGCCA UUAGGCCGAG GGCACGUCUG CCUGGGCGUC A CGC

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Macromolecule #46: 25S rRNA

MacromoleculeName: 25S rRNA / type: rna / ID: 46 / Number of copies: 1
Source (natural)Organism: Nicotiana benthamiana (plant)
Molecular weightTheoretical: 1.097118125 MDa
SequenceString: GCGACCCCAG GUCAGGCGGG ACUACCCGCU GAGUUUAAGC AUAUCAAUAA GCGGAGGAAA AGAAACUUAC AAGGAUUCCC CUAGUAACG GCGAGCGAAC CGGGAACAGC CCAGCCUUAG AAUCGGGCGG CUCCGUCGUC CGAAUUGUAG UCUGGAGAAG C GUCCUCAG ...String:
GCGACCCCAG GUCAGGCGGG ACUACCCGCU GAGUUUAAGC AUAUCAAUAA GCGGAGGAAA AGAAACUUAC AAGGAUUCCC CUAGUAACG GCGAGCGAAC CGGGAACAGC CCAGCCUUAG AAUCGGGCGG CUCCGUCGUC CGAAUUGUAG UCUGGAGAAG C GUCCUCAG CGGCGGACCG GGCCCAAGUC CCCUGGAAGG GGGCGCCAGA GAGGGUGAGA GCCCCGUUGU GCCCGGACCC UG UCGCACC ACGAGGCGCU GUCUACGAGU CGGGUUGUUU GGGAAUGCAG CCCAAAUCGG GCGGUGAAUU CCGUCCAAGG CUA AAUACG GGCGAGAGAC CGAUAGCGAA CAAGUACCGC GAGGGAAAGA UGAAAAGGAC UUUGAAAAGA GAGUCAAAGA GUGC UUGAA AUUGUCGGGA GGGAAGCGGA UGGGGGCCGG CGAUGCGCCC CGGUCGGAUG UGGAACGGUG UUGAGCCGGU CCGCC GAUC GACUCGGGGC GUGGACCAGC GUGGAUUGGG GGGGCGGCCA AAGCCCGGGC UUUUGAUACG CUCGUGGAAC GUCGUC UCC UUGAUUGUGG UAGGCAGCGC GCGCCUCUGG CGUGCUUCGG CAUCUGCGCG CUCCGGACGC UGGCCUGUGG GCUCCCC AU UCGACCCGUC UUGAAACACG GACCAAGGAG UCUGACAUGU GUGCGAGUCA ACGGGCGAGU AAACCCGUAA GGCGUAAG G AAGCUGAUUG GUGGGAUCCC CCUGAGGGGU GCACCGCCGA CCGACCUUGA UCUUCUGUGA AGGGUUCGAG UGUGAGCAU ACCUGUCGGG ACCCGAAAGA UGGUGAACUA UGCCUGAGCG GGGCGAAGCC AGAGGAAACU CUGGUGGAGG CCCGCAGCGA UACUGACGU GCAAAUCGUU CGUCUGACUU GGGUAUAGGG GCGAAAGACU AAUCGAACCG UCUAGUAGCU GGUUCCCUCC G AAGUUUCC CUCAGGAUAG CUGGAGCUCG CGUGCGAGUU CUAUCGGGUA AAGCCAAUGA UUAGAGGCAU CGGGGGCGCA AC GCCCUCG ACCUAUUCUC AAACUUUAAA UAGGUAGGAC GGCGCGGCUG CUUUGUUGAG CCGCGCCACG GAAUCAAGAG CUC CAAGUG GGCCAUUUUU GGUAAGCAGA ACUGGCGAUG CGGGAUGAAC CGGAAGCCGG GUUACGGUGC CAAACUGCGC GCUA ACCUA GAUCCCACAA AGGGUGUUGG UCGAUUAAGA CAGCAGGACG GUGGUCAUGG AAGUCGAAAU CCGCUAAGGA GUGUG UAAC AACUCACCUG CCGAAUCAAC UAGCCCCGAA AAUGGAUGGC GCUUAAGCGC GCGACCUACA CCCGGCCGUC GGGGCA AGU GCCAGGCCCC GAUGAGUAGG AGGGCGCGGC GGUCGCUGCA AAACCUUGGG CGUGAGCCUG GGCGGAGCGG CCGUCGG UG CAGAUCUUGG UGGUAGUAGC AAAUAUUCAA AUGAGAACUU UGAAGGCCGA AGAGGGGAAA GGUUCCAUGU GAACGGCA C UUGCACAUGG GUUAGUCGAU CCUAAGGGUC GGGGGAACCC CGACAGAUAG CGCGUUUCGC GCGUACUCCG AAAGGGAAU CGGGUUAAAA UUCCUGAACC GGGACGUGGC GGUUGACGGC AACGUUAGGA AGUCCGGAGA CGUCGGCGGG GGCCUCGGGA AGAGUUAUC UUUUCUGUUU AACAGCCUGC CCACCCUGGA AACGACUCAG UCGGAGGUAG GGUCCAGCGG CUGGAAGAGC A CCGCACGU CGCGUGGUGU CCGGUGCGCC CCCGGCGGCC CUUGAAAAUC CGGAGGACCG AGUGCCGUCC ACGCCCGGUC GU ACUCAUA ACCGCAUCAG GUCUCCAAGG UGAACAGCCU CUGGUCGAUG GAACAAUGUA GGCAAGGGAA GUCGGCAAAA UGG AUCCGU AACUUCGGGA AAAGGAUUGG CUCUGAGGGC UGGGCACGGG GGUCCCAUUC CCGAACCCGU CGGCUGUCGG UGGA CUGCU CGAGCUGCUC CCGCGGCGAG AGCGGGUCGC CGCGUGCCGG CCGGGGGACG GACUGGGAAC GGUUCCUUCG GGGGC CUUC CCCGGGCGUC GAACAGCCAA CUCAGAACUG GUACGGACAA GGGGAAUCCG ACUGUUUAAU UAAAACAAAG CAUUGC GAU GGUCCCUGCG GAUGUUUACG CAAUGUGAUU UCUGCCCAGU GCUCUGAAUG UCAAAGUGAA GAAAUUCAAC CAAGCGC GG GUAAACGGCG GGAGUAACUA UGACUCUCUU AAGGUAGCCA AAUGCCUCGU CAUCUAAUUA GUGACGCGCA UGAAUGGA U UAACGAGAUU CCCACUGUCC CUGUCUACUA UCCAGCGAAA CCACAGCCAA GGGAACGGGC UUGGCAGAAU CAGCGGGGA AAGAAGACCC UGUUGAGCUU GACUCUAGUC CGACUUUGUG AAAUGACUUG AGAGGUGUAG UAUAAGUGGG AGCCGAAAGG CGAAAGUGA AAUACCACUA CUUUUAACGU UAUUUUACUU AUUCCGUGAA UCGGAAGCGG GGCACUGCCC CUCUUUUUGG A CCCAAGGC UUGCUUGCAG GCCGAUCCGG GCGGAAGACA UUGUCAGGUG GGGAGUUUGG CUGGGGCGGC ACAUCUGUUA AA AGAUAAC GCAGGUGUCC UAAGAUGAGC UCAACGAGAA CAGAAAUCUC GUGUGGAACA GAAGGGUAAA AGCUCGUUUG AUU CUGAUU UCCAGUACGA AUACGAACCG UGAAAGCGUG GCCUAACGAU CCUUUAGACC UUCGGAAUUC GAAGCUAGAG GUGU CAGAA AAGUUACCAC AGGGAUAACU GGCUUGUGGC AGCCAAGCGU UCAUAGCGAC GUUGCUUUUU GAUCCUUCGA UGUCG GCUC UUCCUAUCAU UGUGAAGCAG AAUUCACCAA GUGUUGGAUU GUUCACCCAC CAAUAGGGAA CGUGAGCUGG GUUUAG ACC GUCGUGAGAC AGGUUAGUUU UACCCUACUG AUGACAGUGU CGCAAUAGUA AUUCAACCUA GUACGAGAGG AACCGUU GA UUCGCACAAU UGGUCAUCGC GCUUGGUUGA AAAGCCAGUG GCGCGAAGCU ACCGUGCGCU GGAUUAUGAC UGAACGCC U CUAAGUCAGA AUCCGGGCUA GAAGCGACGC AUGCGCCCGC CGUCCGCUUG CCGACCCGCA GUAGGGGCCU UUGGCCCCC AAGGGCACGU GUCGUUGGCU AAGUCAUCGC GGCGGAAGAG CCGCGGUGGC CGCCUUGAAG UACAAUUUCC AUCGAGCGGC GGGUAGAAU CCUUUGCAGA CGACUUAAAU ACGCGACGGG GUAUUGUAAG UGGCAGAGUG GCCUUGCUGC CACGAUCCAC U GAGAUUCA GCCCUUUGUC GCUCCGAUUC GU

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON I (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 269862
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

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