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Basic information

Entry
Database: EMDB / ID: EMD-65277
TitleThe structure of DbOR67d-DbOrco in the Z11-bound state
Map data
Sample
  • Complex: The structure of DbOR67d-DbOrco in the Z11-bound state
    • Protein or peptide: OR67d in Drosophila Bipectinata
    • Protein or peptide: Orco in Drosophila Bipectinata
  • Ligand: [(~{Z})-icos-11-enyl] ethanoate
KeywordsOdorant Receptor / Complex / channel / MEMBRANE PROTEIN
Biological speciesDrosophila bipectinata (fry)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.8 Å
AuthorsWang J / Guo J
Funding support China, 1 items
OrganizationGrant numberCountry
Ministry of Science and Technology (MoST, China)2020YFA0908501 China
CitationJournal: Cell Res. / Year: 2026
Title: Cryo-EM structures of Drosophila OR67d-Orco complexes reveal insect pheromone sensing mechanism.
Authors: Wang J / Yang C / Chang S / Jiao D / Lin J / Yang X / Cai W / Ma D / Ding ZJ / Huang J / Huang J / Fan M / Hu M / Wang Y / Xu H / Su N / Guo J
History
DepositionJul 5, 2025-
Header (metadata) releaseJul 1, 2026-
Map releaseJul 1, 2026-
UpdateJul 1, 2026-
Current statusJul 1, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_65277.png

Downloads & links

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Map

FileReleased
Voxel sizeX=Y=Z: 0.93 Å
Density
Contour LevelBy AUTHOR: 0.0137
Minimum - Maximum-0.035713617 - 0.07502674
Average (Standard dev.)0.000015422147 (±0.0028861072)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions240240240
Spacing240240240
CellA=B=C: 223.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : The structure of DbOR67d-DbOrco in the Z11-bound state

EntireName: The structure of DbOR67d-DbOrco in the Z11-bound state
Components
  • Complex: The structure of DbOR67d-DbOrco in the Z11-bound state
    • Protein or peptide: OR67d in Drosophila Bipectinata
    • Protein or peptide: Orco in Drosophila Bipectinata
  • Ligand: [(~{Z})-icos-11-enyl] ethanoate

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Supramolecule #1: The structure of DbOR67d-DbOrco in the Z11-bound state

SupramoleculeName: The structure of DbOR67d-DbOrco in the Z11-bound state
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2
Source (natural)Organism: Drosophila bipectinata (fry)

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Macromolecule #1: OR67d in Drosophila Bipectinata

MacromoleculeName: OR67d in Drosophila Bipectinata / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Drosophila bipectinata (fry)
Molecular weightTheoretical: 44.251719 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: KLTPVDRYLR IIKVIRFFVG FCGNDVANPN FKMWWLTYLV LSAIGLFYAC TGYTIYRGVV IDGDLTIILQ AMAMVGSATQ GLTKLLVTA NLAPLLRHIQ YSYEDIYREY GAKGGEYTEC LERRIRITWR LMLSFLCPCL MSMVCIVSFP IFYLVVYKKK I MVMQFLMP ...String:
KLTPVDRYLR IIKVIRFFVG FCGNDVANPN FKMWWLTYLV LSAIGLFYAC TGYTIYRGVV IDGDLTIILQ AMAMVGSATQ GLTKLLVTA NLAPLLRHIQ YSYEDIYREY GAKGGEYTEC LERRIRITWR LMLSFLCPCL MSMVCIVSFP IFYLVVYKKK I MVMQFLMP LIDENTDGGY MILSAVHVGL IAFGGFGNYG GDMYLFLFVT NLTLIKDIFC IKIKELNEVV LKRTEYEQMR LL LFDLVAW HQKYQNILLT TRRIYSIVLF VQLSTTCMSM LCTIACVFLH VWPAAPIYLL YAGTMLYAFC GLGTAVEISN DAL MREIYS GCLWYELPVK EEKIIVLMLA KAQTESYLTA ANMWPLSMNT ALQLTKGMYS FTMMMLTYLG

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Macromolecule #2: Orco in Drosophila Bipectinata

MacromoleculeName: Orco in Drosophila Bipectinata / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Drosophila bipectinata (fry)
Molecular weightTheoretical: 53.974578 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MTTSMQPSKY TGLVADLMPN IKAMKYSGLF MHNFTGGSAF VKKVYSSVHL VLLLLQFVFI LVNMALNAEE VNELSGNTIT TLFFTHCIT KFIYLAVNQK NFYRTLNIWN QVNTHPLFAE SDARYHSIAL AKMRKLFFLV MLTTVASATA WTTITFFGDS V KMVVDHET ...String:
MTTSMQPSKY TGLVADLMPN IKAMKYSGLF MHNFTGGSAF VKKVYSSVHL VLLLLQFVFI LVNMALNAEE VNELSGNTIT TLFFTHCIT KFIYLAVNQK NFYRTLNIWN QVNTHPLFAE SDARYHSIAL AKMRKLFFLV MLTTVASATA WTTITFFGDS V KMVVDHET NSSVPVEIPR LPIKSFYPWN AGHGIFYMIS FAFQIYYILF SMIHSNLCDV MFCSWLIFAC EQLQHLKGIM KP LMELSAS LDTYRPNSAA LFRSLSANSK SELIHNEEKD PGADMDMSGV YSSKADWGAQ FRAPSTLQSF GGNGGIVNGA NPN GLTKKQ EMMVRSAIKY WVERHKHVVR LVAAIGDTYG AALLLHMLTS TIKLTLLAYQ ATKITGVNVY AFTVIGYLGY ALAQ VFHFC IFGNRLIEES SSVMEAAYSC HWYDGSEEAK TFVQIVCQQC QKAMSISGAK FFTVSLDLFA SVLGAVVTYF MVLVQ LK

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Macromolecule #3: [(~{Z})-icos-11-enyl] ethanoate

MacromoleculeName: [(~{Z})-icos-11-enyl] ethanoate / type: ligand / ID: 3 / Number of copies: 1 / Formula: A1ETW
Molecular weightTheoretical: 338.568 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: DIFFRACTION / Nominal defocus max: 1.6 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 594089
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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