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Basic information

Entry
Database: EMDB / ID: EMD-65137
TitleCryoEM struccture of the type III secretion system gatekeeper protein InvE by cryo-electron microscopy.
Map data
Sample
  • Organelle or cellular component: Invasion protein InvE
    • Protein or peptide: Invasion protein InvE
Keywordstetramer / T3SS / gatekeeper / Regulation of Protein Secretion Order / CYTOSOLIC PROTEIN
Biological speciesSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (bacteria) / Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.07 Å
AuthorsCheng XQ / Wu M / Jiang WX / Xing Q
Funding support China, 2 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32371277 China
National Natural Science Foundation of China (NSFC)32301028 China
CitationJournal: To Be Published
Title: CryoEM struccture of the type III secretion system gatekeeper protein InvE by cryo-electron microscopy.
Authors: Cheng XQ / Wu M / Jiang WX / Xing Q
History
DepositionJun 23, 2025-
Header (metadata) releaseJul 1, 2026-
Map releaseJul 1, 2026-
UpdateJul 1, 2026-
Current statusJul 1, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_65137.png

Downloads & links

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Map

FileReleased
Voxel sizeX=Y=Z: 0.567 Å
Density
Contour LevelBy AUTHOR: 0.0297
Minimum - Maximum-0.5030693 - 0.6655759
Average (Standard dev.)0.00009867313 (±0.015032726)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 226.79999 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Invasion protein InvE

EntireName: Invasion protein InvE
Components
  • Organelle or cellular component: Invasion protein InvE
    • Protein or peptide: Invasion protein InvE

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Supramolecule #1: Invasion protein InvE

SupramoleculeName: Invasion protein InvE / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (bacteria)
Molecular weightTheoretical: 135.6 kDa/nm

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Macromolecule #1: Invasion protein InvE

MacromoleculeName: Invasion protein InvE / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
Strain: SR-11 LT2 / SGSC1412 / ATCC 700720 TML
Molecular weightTheoretical: 42.47541 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MIPGSTSGIS FSRILSRQTS HQDATQHTDA QQAEIQQAAE DSSPGAEVQK FVQSTDEMSA ALAQFRNRRD YEKKSSNLSN SFERVLEDE ALPKAKQILK LISVHGGALE DFLRQARSLF PDPSDLVLVL RELLRRKDLE EIVRKKLESL LKHVEEQTDP K TLKAGINC ...String:
MIPGSTSGIS FSRILSRQTS HQDATQHTDA QQAEIQQAAE DSSPGAEVQK FVQSTDEMSA ALAQFRNRRD YEKKSSNLSN SFERVLEDE ALPKAKQILK LISVHGGALE DFLRQARSLF PDPSDLVLVL RELLRRKDLE EIVRKKLESL LKHVEEQTDP K TLKAGINC ALKARLFGKT LSLKPGLLRA SYRQFIQSES HEVEIYSDWI ASYGYQRRLV VLDFIEGSLL TDIDANDASC SR LEFGQLL RRLTQLKMLR SADLLFVSTL LSYSFTKAFN AEESSWLLLM LSLLQQPHEV DSLLADIIGL NALLLSHKEH ASF LQIFYQ VCKAIPSSLF YEEYWQEELL MALRSMTDIA YKHEMAEQRR TIEKLS

UniProtKB: Invasion protein InvE

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration4 mg/mL
BufferpH: 8
Component:
ConcentrationFormulaName
20.0 mMTris2-Amino-2-(hydroxymethyl)-1,3-propanediol
200.0 mMNaClsodium chloride
GridModel: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 50 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 281.15 K / Instrument: FEI VITROBOT MARK III

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 43.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 5.0 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.07 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 213045
Initial angle assignmentType: NOT APPLICABLE / Software - Name: cryoSPARC
Final angle assignmentType: NOT APPLICABLE / Software - Name: cryoSPARC
FSC plot (resolution estimation)

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