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- EMDB-65037: Recombinantly expressed VCP containing the A232E mutation -

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Basic information

Entry
Database: EMDB / ID: EMD-65037
TitleRecombinantly expressed VCP containing the A232E mutation
Map data
Sample
  • Complex: Recombinantly expressed VCP containing the A232E mutation
KeywordsVCP / ATPase / Multisystem proteinopathy mutations / PROTEIN BINDING
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.43 Å
AuthorsPan M / Zheng Q / Liu L
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: To Be Published
Title: Recombinantly expressed VCP containing the A232E mutation
Authors: Pan M / Zheng Q / Liu L
History
DepositionJun 11, 2025-
Header (metadata) releaseJun 17, 2026-
Map releaseJun 17, 2026-
UpdateJun 17, 2026-
Current statusJun 17, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_65037.png

Downloads & links

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Map

FileDownload / File: emd_65037.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 320 pix.
= 343.36 Å		1.07 Å/pix.
x 320 pix.
= 343.36 Å		1.07 Å/pix.
x 320 pix.
= 343.36 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.073 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.01
Minimum - Maximum-0.04621936 - 0.084442094
Average (Standard dev.)0.000027156391 (±0.0029091572)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 343.36 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_65037_msk_1.map
Projections & Slices
AxesZYX

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Histogram

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Additional map: #1

Fileemd_65037_additional_1.map
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Histogram

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Half map: #1

Fileemd_65037_half_map_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_65037_half_map_2.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Recombinantly expressed VCP containing the A232E mutation

EntireName: Recombinantly expressed VCP containing the A232E mutation
Components
  • Complex: Recombinantly expressed VCP containing the A232E mutation

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Supramolecule #1: Recombinantly expressed VCP containing the A232E mutation

SupramoleculeName: Recombinantly expressed VCP containing the A232E mutation
type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.43 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 104394
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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