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- EMDB-64774: Cryo-EM density map of the 96-nm repeat of the human respiratory ... -

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Basic information

Entry
Database: EMDB / ID: EMD-64774
TitleCryo-EM density map of the 96-nm repeat of the human respiratory doublet microtubule
Map data
Sample
  • Complex: Doublet microtubule and associated proteins
Keywordshuman respiratory doublet microtubule / STRUCTURAL PROTEIN
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 17.7 Å
AuthorsShuo Y / Miao G
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: To Be Published
Title: Loss-of-function mutations in CCDC114 disrupt ODA docking and induce actin cytoskeletal remodeling in primary ciliary dyskinesia
Authors: Chunxiao H / Ting L / Shuo Y / Yuan J / Feng Y / Zhangqi X / Junhua X / Lei W / Miao G / Shanshan X / Tianhua Z
History
DepositionMay 23, 2025-
Header (metadata) releaseMar 25, 2026-
Map releaseMar 25, 2026-
UpdateMar 25, 2026-
Current statusMar 25, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_64774.png

Downloads & links

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Map

FileDownload / File: emd_64774.map.gz / Format: CCP4 / Size: 1.3 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.86 Å/pix.
x 704 pix.
= 1309.44 Å		1.86 Å/pix.
x 704 pix.
= 1309.44 Å		1.86 Å/pix.
x 704 pix.
= 1309.44 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.86 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.005
Minimum - Maximum-0.013658977 - 0.031310067
Average (Standard dev.)0.0004829755 (±0.0024171693)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions704704704
Spacing704704704
CellA=B=C: 1309.4401 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_64774_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: #1

Fileemd_64774_additional_1.map
Projections & Slices
AxesZYX

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Density Histograms

Histogram

Histogram (log scale)

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Additional map: #2

Fileemd_64774_additional_2.map
Projections & Slices
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Half map: #1

Fileemd_64774_half_map_1.map
Projections & Slices
AxesZYX

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Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_64774_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Doublet microtubule and associated proteins

EntireName: Doublet microtubule and associated proteins
Components
  • Complex: Doublet microtubule and associated proteins

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Supramolecule #1: Doublet microtubule and associated proteins

SupramoleculeName: Doublet microtubule and associated proteins / type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 130000
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: EMDB MAP
EMDB ID:

26624

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 17.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 4034
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 4.0)
Final angle assignmentType: ANGULAR RECONSTITUTION
FSC plot (resolution estimation)

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