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- EMDB-64722: The Chlamydomonas reinhardtii bicarbonate transporter LciA -

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Basic information

Entry
Database: EMDB / ID: EMD-64722
TitleThe Chlamydomonas reinhardtii bicarbonate transporter LciA
Map data
Sample
  • Complex: Low-CO2 inducible protein LciA
    • Protein or peptide: Low-CO2 inducible protein LCIA
KeywordsChlamydomonas reinhardtii / bicarbonate transporters / LciA / CO2-concentrating mechanism / TRANSPORT PROTEIN
Function / homologyformate transport / formate transmembrane transporter activity / Formate/nitrite transporter / Formate/nitrite transporter / Aquaporin-like / plasma membrane / Low-CO2 inducible protein LCIA
Function and homology information
Biological speciesChlamydomonas reinhardtii (plant)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.37 Å
AuthorsYang Z / Guo J / Zhang P
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32401000 China
CitationJournal: To Be Published
Title: Structure-guided engineering of novel bicarbonate transporters based on Chlamydomonas reinhardtii LciA
Authors: Yang Z / Guo J / Zhang P
History
DepositionMay 19, 2025-
Header (metadata) releaseNov 26, 2025-
Map releaseNov 26, 2025-
UpdateNov 26, 2025-
Current statusNov 26, 2025Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_64722.png

Downloads & links

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Map

FileDownload / File: emd_64722.map.gz / Format: CCP4 / Size: 83.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.93 Å/pix.
x 280 pix.
= 260.96 Å		0.93 Å/pix.
x 280 pix.
= 260.96 Å		0.93 Å/pix.
x 280 pix.
= 260.96 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.932 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.7
Minimum - Maximum-2.4611795 - 4.0121603
Average (Standard dev.)-0.00087280164 (±0.11669119)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions280280280
Spacing280280280
CellA=B=C: 260.96 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_64722_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_64722_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Low-CO2 inducible protein LciA

EntireName: Low-CO2 inducible protein LciA
Components
  • Complex: Low-CO2 inducible protein LciA
    • Protein or peptide: Low-CO2 inducible protein LCIA

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Supramolecule #1: Low-CO2 inducible protein LciA

SupramoleculeName: Low-CO2 inducible protein LciA / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Chlamydomonas reinhardtii (plant)

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Macromolecule #1: Low-CO2 inducible protein LCIA

MacromoleculeName: Low-CO2 inducible protein LCIA / type: protein_or_peptide / ID: 1 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 32.063416 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MVTSNGNGNG HFQAATTPVP PTPAPVAVSA PVRAVSVLTP PQVYENAINV GAYKAGLTPL ATFVQGIQAG AYIAFGAFLA ISVGGNIPG VAAANPGLAK LLFALVFPVG LSMVTNCGAE LFTGNTMMLT CALIEKKATW GQLLKNWSVS YFGNFVGSIA M VAAVVATG ...String:
MVTSNGNGNG HFQAATTPVP PTPAPVAVSA PVRAVSVLTP PQVYENAINV GAYKAGLTPL ATFVQGIQAG AYIAFGAFLA ISVGGNIPG VAAANPGLAK LLFALVFPVG LSMVTNCGAE LFTGNTMMLT CALIEKKATW GQLLKNWSVS YFGNFVGSIA M VAAVVATG CLTTNTLPVQ MATLKANLGF TEVLSRSILC NWLVCCAVWS ASAATSLPGR ILALWPCITA FVAIGLEHSV AN MFVIPLG MMLGAEVTWS QFFFNNLIPV TLGNTIAGVL MMAIAYSISF GSLGKSAKPA TAKLDYKDDD DK

UniProtKB: Low-CO2 inducible protein LCIA

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration27 mg/mL
BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.37 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 169175
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING
FSC plot (resolution estimation)

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