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基本情報
登録情報 | ![]() | |||||||||
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タイトル | GPR133-Gain-miniG13 complex | |||||||||
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![]() | GPCR / G12/G13 / Complex / Stachel / MEMBRANE PROTEIN | |||||||||
機能・相同性 | ![]() D5 dopamine receptor binding / regulation of skeletal muscle contraction / regulation of fibroblast migration / Rho-activating G protein-coupled receptor signaling pathway / regulation of small GTPase mediated signal transduction / NRAGE signals death through JNK / branching involved in blood vessel morphogenesis / CDC42 GTPase cycle / regulation of postsynapse assembly / Rho protein signal transduction ...D5 dopamine receptor binding / regulation of skeletal muscle contraction / regulation of fibroblast migration / Rho-activating G protein-coupled receptor signaling pathway / regulation of small GTPase mediated signal transduction / NRAGE signals death through JNK / branching involved in blood vessel morphogenesis / CDC42 GTPase cycle / regulation of postsynapse assembly / Rho protein signal transduction / RAC1 GTPase cycle / guanyl-nucleotide exchange factor activity / brush border membrane / G protein-coupled receptor activity / platelet activation / regulation of blood pressure / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / G-protein beta/gamma-subunit complex binding / Olfactory Signaling Pathway / Activation of the phototransduction cascade / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Thromboxane signalling through TP receptor / G protein-coupled acetylcholine receptor signaling pathway / G-protein activation / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / adenylate cyclase-activating G protein-coupled receptor signaling pathway / Prostacyclin signalling through prostacyclin receptor / G beta:gamma signalling through CDC42 / Glucagon signaling in metabolic regulation / G beta:gamma signalling through BTK / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / ADP signalling through P2Y purinoceptor 12 / Sensory perception of sweet, bitter, and umami (glutamate) taste / photoreceptor disc membrane / Glucagon-type ligand receptors / Adrenaline,noradrenaline inhibits insulin secretion / Vasopressin regulates renal water homeostasis via Aquaporins / G alpha (z) signalling events / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / ADP signalling through P2Y purinoceptor 1 / cellular response to catecholamine stimulus / ADORA2B mediated anti-inflammatory cytokines production / G beta:gamma signalling through PI3Kgamma / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / adenylate cyclase-activating dopamine receptor signaling pathway / melanosome / GPER1 signaling / Inactivation, recovery and regulation of the phototransduction cascade / cellular response to prostaglandin E stimulus / G-protein beta-subunit binding / heterotrimeric G-protein complex / G alpha (12/13) signalling events / sensory perception of taste / extracellular vesicle / regulation of cell shape / signaling receptor complex adaptor activity / Thrombin signalling through proteinase activated receptors (PARs) / retina development in camera-type eye / G protein activity / GTPase binding / Ca2+ pathway / fibroblast proliferation / High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells / G alpha (i) signalling events / G alpha (s) signalling events / phospholipase C-activating G protein-coupled receptor signaling pathway / G alpha (q) signalling events / in utero embryonic development / Ras protein signal transduction / cell differentiation / Extra-nuclear estrogen signaling / cell surface receptor signaling pathway / cell population proliferation / postsynapse / nuclear speck / G protein-coupled receptor signaling pathway / lysosomal membrane / focal adhesion / GTPase activity / synapse / GTP binding / protein-containing complex binding / signal transduction / extracellular exosome / nucleoplasm / metal ion binding / nucleus / membrane / plasma membrane / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.51 Å | |||||||||
![]() | Xi Y / Pu X / Ping Y | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Cryo-EM structural elucidation and molecular mechanism of the GPR133-G13 signaling complex. 著者: Xuanyu Pu / Yue-Tong Xi / Meng-Xin Wang / Daolai Zhang / Yu-Qi Ping / Peng Xiao / Jin-Peng Sun / ![]() 要旨: GPR133 is an adhesion-class G protein-coupled receptor (GPCR) that has recently been de-orphanized. Its functions are complex and multifaceted. While GPR133 is primarily recognized for coupling with ...GPR133 is an adhesion-class G protein-coupled receptor (GPCR) that has recently been de-orphanized. Its functions are complex and multifaceted. While GPR133 is primarily recognized for coupling with the Gs subunit to mediate elevated intracellular cAMP levels, its potential engagement with alternative signaling pathways remains poorly characterized. In our experiments, we demonstrated that GPR133 exhibits constitutive self-activation via its Stachel sequence as an adhesion GPCR, enabling activation of downstream G13 signaling. We reconstituted the GPR133-GAIN-miniGα13 complex in vitro and resolved its cryo-electron microscopy structure at a resolution of 3.51 Å. Detailed structural comparisons between the GPR133-GAIN-miniGα13 complex and the previously resolved GPR133-CTF-Gs structure highlighted both conserved and different features. These findings provide critical insights into the signal transduction mechanisms of GPR133 and lay a foundation for targeted therapeutic strategies. | |||||||||
履歴 |
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 52.8 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 18.1 KB 18.1 KB | 表示 表示 | ![]() |
画像 | ![]() | 34.9 KB | ||
Filedesc metadata | ![]() | 6.2 KB | ||
その他 | ![]() ![]() | 55.4 MB 55.4 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 837 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 836.6 KB | 表示 | |
XML形式データ | ![]() | 11.9 KB | 表示 | |
CIF形式データ | ![]() | 14.1 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 9v0uMC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 0.93 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: #2
ファイル | emd_64672_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_64672_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
-全体 : GPR133-Gain-miniG13-Gbeta1-Ggamma2 complex
全体 | 名称: GPR133-Gain-miniG13-Gbeta1-Ggamma2 complex |
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要素 |
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-超分子 #1: GPR133-Gain-miniG13-Gbeta1-Ggamma2 complex
超分子 | 名称: GPR133-Gain-miniG13-Gbeta1-Ggamma2 complex / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all |
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由来(天然) | 生物種: ![]() |
-分子 #1: Adhesion G-protein coupled receptor D1
分子 | 名称: Adhesion G-protein coupled receptor D1 / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 65.688828 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: HPIITNLTEE RKTFQSPGVI LSYLQNVSLS LPSKSLSEQT ALNLTKTFLK AVGEILLLPG WIALSEDSAV VLSLIDTIDT VMGHVSSNL HGSTPQVTVE GSSAMAEFSV AKILPKTVNS SHYRFPAHGQ SFIQIPHEAF HRHAWSTVVG LLYHSMHYYL N NIWPAHTK ...文字列: HPIITNLTEE RKTFQSPGVI LSYLQNVSLS LPSKSLSEQT ALNLTKTFLK AVGEILLLPG WIALSEDSAV VLSLIDTIDT VMGHVSSNL HGSTPQVTVE GSSAMAEFSV AKILPKTVNS SHYRFPAHGQ SFIQIPHEAF HRHAWSTVVG LLYHSMHYYL N NIWPAHTK IAEAMHHQDC LLFATSHLIS LEVSPPPTLS QNLSGSPLIT VHLKHRLTRK QHSEATNSSN RVFVYCAFLD FS SGEGVWS NHGCALTRGN LTYSVCRCTH LTNFAILMQV VPLELARGHQ VALSSISYVG CSLSVLCLVA TLVTFAVLSS VST IRNQRY HIHANLSFAV LVAQVLLLIS FRLEPGTTPC QVMAVLLHYF FLSAFAWMLV EGLHLYSMVI KVFGSEDSKH RYYY GMGWG FPLLICIISL SFAMDSYGTS NNCWLSLASG AIWAFVAPAL FVIVVNIGIL IAVTRVISQI SADNYKIHGD PSAFK LTAK AVAVLLPILG TSWVFGVLAV NGCAVVFQYM FATLNSLQGL FIFLFHCLLN SEVRAAFKHK TKVWSLTSSS ARTSNA KPF HSDLMNGTRP GMASTKLSPW DKSSHSAHRV DLSAV UniProtKB: Adhesion G-protein coupled receptor D1 |
-分子 #2: Guanine nucleotide-binding protein subunit alpha-13,Isoform 2 of ...
分子 | 名称: Guanine nucleotide-binding protein subunit alpha-13,Isoform 2 of Guanine nucleotide-binding protein subunit alpha-13 タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 26.705596 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: MMGSTVSAED KAAAERSKEI DKCLSREKTY VKRLVKILLL GADNSGKSTF LKQMRIIHGG SGGSGGTKGI HEYDFEIKNV PFKMVDVGG QRSERKRWFE CFDSVTSILF LVDSSDFNRL TESLNDFETI VNNRVFSNVS IILFLNKTDL LEEKVQIVSI K DYFLEFEG ...文字列: MMGSTVSAED KAAAERSKEI DKCLSREKTY VKRLVKILLL GADNSGKSTF LKQMRIIHGG SGGSGGTKGI HEYDFEIKNV PFKMVDVGG QRSERKRWFE CFDSVTSILF LVDSSDFNRL TESLNDFETI VNNRVFSNVS IILFLNKTDL LEEKVQIVSI K DYFLEFEG DPHCLRDVQK FLVECFRNKR RDQQQKPLYH HFTTAINTEN ARLIFRDVKD TILHDNLKQL MLQ UniProtKB: Guanine nucleotide-binding protein subunit alpha-13, Guanine nucleotide-binding protein subunit alpha-13, Guanine nucleotide-binding protein subunit alpha-13 |
-分子 #3: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
分子 | 名称: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 7.861143 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: MASNNTASIA QARKLVEQLK MEANIDRIKV SKAAADLMAY CEAHAKEDPL LTPVPASENP FREKKFFCAI L UniProtKB: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 |
-分子 #4: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
分子 | 名称: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 37.784301 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: GSLLQSELDQ LRQEAEQLKN QIRDARKACA DATLSQITNN IDPVGRIQMR TRRTLRGHLA KIYAMHWGTD SRLLVSASQD GKLIIWDSY TTNKVHAIPL RSSWVMTCAY APSGNYVACG GLDNICSIYN LKTREGNVRV SRELAGHTGY LSCCRFLDDN Q IVTSSGDT ...文字列: GSLLQSELDQ LRQEAEQLKN QIRDARKACA DATLSQITNN IDPVGRIQMR TRRTLRGHLA KIYAMHWGTD SRLLVSASQD GKLIIWDSY TTNKVHAIPL RSSWVMTCAY APSGNYVACG GLDNICSIYN LKTREGNVRV SRELAGHTGY LSCCRFLDDN Q IVTSSGDT TCALWDIETG QQTTTFTGHT GDVMSLSLAP DTRLFVSGAC DASAKLWDVR EGMCRQTFTG HESDINAICF FP NGNAFAT GSDDATCRLF DLRADQELMT YSHDNIICGI TSVSFSKSGR LLLAGYDDFN CNVWDALKAD RAGVLAGHDN RVS CLGVTD DGMAVATGSW DSFLKIWN UniProtKB: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | TFS KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON IV (4k x 4k) 平均電子線量: 60.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.0 µm / 最小 デフォーカス(公称値): 1.0 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |