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- EMDB-64160: human Ribonuclease MRP state 2 -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-64160
Titlehuman Ribonuclease MRP state 2
Map data
Sample
  • Complex: hsRNaseMRP
    • RNA: x 1 types
    • Protein or peptide: x 11 types
  • Ligand: x 1 types
KeywordsRNase MRP / RNA BINDING PROTEIN/RNA / RNA BINDING PROTEIN-RNA complex
Function / homology
Function and homology information


multimeric ribonuclease P complex / nucleolar ribonuclease P complex / ribonuclease P RNA binding / ribonuclease MRP complex / ribonuclease P complex / tRNA decay / ribonuclease P activity / tRNA processing in the nucleus / tRNA 5'-leader removal / tRNA processing ...multimeric ribonuclease P complex / nucleolar ribonuclease P complex / ribonuclease P RNA binding / ribonuclease MRP complex / ribonuclease P complex / tRNA decay / ribonuclease P activity / tRNA processing in the nucleus / tRNA 5'-leader removal / tRNA processing / negative regulation of neuron differentiation / positive regulation of Notch signaling pathway / RNA processing / Major pathway of rRNA processing in the nucleolus and cytosol / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / fibrillar center / centriolar satellite / rRNA processing / nucleolus / : / RNA binding / nucleoplasm / nucleus / cytoplasm
Similarity search - Function
Nucleolus and neural progenitor protein-like, N-terminal domain / Protein of unknown function DUF4674 / : / Nucleolus and neural progenitor protein, N-terminal / Ribonuclease MRP subunit P24-like / Ribonuclease P protein subunit Rpp38 / Ribonuclease P protein subunit Rpp40 / Ribonuclease P 40kDa (Rpp40) subunit / : / Ribonuclease P/MRP protein subunit Pop5 ...Nucleolus and neural progenitor protein-like, N-terminal domain / Protein of unknown function DUF4674 / : / Nucleolus and neural progenitor protein, N-terminal / Ribonuclease MRP subunit P24-like / Ribonuclease P protein subunit Rpp38 / Ribonuclease P protein subunit Rpp40 / Ribonuclease P 40kDa (Rpp40) subunit / : / Ribonuclease P/MRP protein subunit Pop5 / Pop1, N-terminal / POPLD domain / Ribonucleases P/MRP protein subunit Pop1 / : / Ribonucleases P/MRP protein subunit POP1, N-terminal / POPLD (NUC188) domain / POP1 C-terminal domain / Ribonucleases P/MRP protein subunit Rpp20/Pop7 / Rpp20 subunit of nuclear RNase MRP and P / RNase P subunit Pop5/Rpp14/Rnp2-like / RNase P subunit Pop5/Rpp14/Rnp2-like domain superfamily / Rpp14/Pop5 family / RNase P subunit p30 / Ribonuclease P/MRP subunit Rpp29 / RNase P subunit p30 / Ribonuclease P protein subunit Rpp29/RNP1 / Ribonuclease P/MRP subunit Rpp29 superfamily / Ribonuclease P/MRP, subunit p29 / A domain found in a protein subunit of human RNase MRP and RNase P ribonucleoprotein complexes and archaeal proteins. / Rof/RNase P-like / DNA/RNA-binding protein Alba-like / Alba / Alba-like domain superfamily / Polymerase/histidinol phosphatase-like / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family / 50S ribosomal protein L30e-like
Similarity search - Domain/homology
Ribonuclease P protein subunit p20 / Ribonuclease P protein subunit p40 / Ribonuclease P protein subunit p14 / Ribonuclease P protein subunit p29 / Ribonuclease P protein subunit p38 / Ribonuclease P protein subunit p30 / Ribonuclease MRP protein subunit p24 / Ribonuclease MRP subunit P64 / Ribonuclease P/MRP protein subunit POP5 / Ribonucleases P/MRP protein subunit POP1 / Ribonuclease P protein subunit p25
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.93 Å
AuthorsZhou B / Lan P / Wu J / Lei M
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: To Be Published
Title: Structural insights into the evolution of RNase P and RNase MRP ribonucleoprotein complexes
Authors: Zhou B / Lan P / Wu J / Lei M
History
DepositionApr 14, 2025-
Header (metadata) releaseApr 22, 2026-
Map releaseApr 22, 2026-
UpdateApr 22, 2026-
Current statusApr 22, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_64160.png

Downloads & links

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Map

FileDownload / File: emd_64160.map.gz / Format: CCP4 / Size: 107.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.1 Å/pix.
x 304 pix.
= 334.4 Å		1.1 Å/pix.
x 304 pix.
= 334.4 Å		1.1 Å/pix.
x 304 pix.
= 334.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.1 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.003
Minimum - Maximum-0.014759159 - 0.033910785
Average (Standard dev.)0.0000067819055 (±0.0013140587)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions304304304
Spacing304304304
CellA=B=C: 334.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_64160_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_64160_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : hsRNaseMRP

EntireName: hsRNaseMRP
Components
  • Complex: hsRNaseMRP
    • RNA: RNA (270-MER)
    • Protein or peptide: Ribonucleases P/MRP protein subunit POP1
    • Protein or peptide: Ribonuclease P protein subunit p38
    • Protein or peptide: Ribonuclease P protein subunit p29
    • Protein or peptide: Ribonuclease P/MRP protein subunit POP5
    • Protein or peptide: Ribonuclease P protein subunit p25
    • Protein or peptide: Ribonuclease P protein subunit p20
    • Protein or peptide: Ribonuclease P protein subunit p14
    • Protein or peptide: Ribonuclease P protein subunit p30
    • Protein or peptide: UPF0711 protein C18orf21
    • Protein or peptide: Ribonuclease P protein subunit p40
    • Protein or peptide: Nucleolus and neural progenitor protein
  • Ligand: ZINC ION

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Supramolecule #1: hsRNaseMRP

SupramoleculeName: hsRNaseMRP / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#12
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: RNA (270-MER)

MacromoleculeName: RNA (270-MER) / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 86.578078 KDa
SequenceString: UGGUUCGUGC UGAAGGCCUG UAUCCUAGGC UACACACUGA GGACUCUGUU CCUCCCCUUU CCGCCUAGGG GAAAGUCCCC GGACCUCGG GCAGAGAGUG CCACGUGCAU ACGCACGUAG ACAUUCCCCG CUUCCCACUC CAAAGUCCGC CAAGAAGCGU A UCCCGCUG ...String:
UGGUUCGUGC UGAAGGCCUG UAUCCUAGGC UACACACUGA GGACUCUGUU CCUCCCCUUU CCGCCUAGGG GAAAGUCCCC GGACCUCGG GCAGAGAGUG CCACGUGCAU ACGCACGUAG ACAUUCCCCG CUUCCCACUC CAAAGUCCGC CAAGAAGCGU A UCCCGCUG AGCGGCGUGG CGCGGGGGCG UCAUCCGUCA GCUCCCUCUA GUUACGCAGG CAGUGCGUGU CCGCGCACCA AC CACACGG GGCUCAUUCU CAGCGCGGCU GUU

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Macromolecule #2: Ribonucleases P/MRP protein subunit POP1

MacromoleculeName: Ribonucleases P/MRP protein subunit POP1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 114.896141 KDa
SequenceString: MSNAKERKHA KKMRNQPTNV TLSSGFVADR GVKHHSGGEK PFQAQKQEPH PGTSRQRQTR VNPHSLPDPE VNEQSSSKGM FRKKGGWKA GPEGTSQEIP KYITASTFAQ ARAAEISAML KAVTQKSSNS LVFQTLPRHM RRRAMSHNVK RLPRRLQEIA Q KEAEKAVH ...String:
MSNAKERKHA KKMRNQPTNV TLSSGFVADR GVKHHSGGEK PFQAQKQEPH PGTSRQRQTR VNPHSLPDPE VNEQSSSKGM FRKKGGWKA GPEGTSQEIP KYITASTFAQ ARAAEISAML KAVTQKSSNS LVFQTLPRHM RRRAMSHNVK RLPRRLQEIA Q KEAEKAVH QKKEHSKNKC HKARRCHMNR TLEFNRRQKK NIWLETHIWH AKRFHMVKKW GYCLGERPTV KSHRACYRAM TN RCLLQDL SYYCCLELKG KEEEILKALS GMCNIDTGLT FAAVHCLSGK RQGSLVLYRV NKYPREMLGP VTFIWKSQRT PGD PSESRQ LWIWLHPTLK QDILEEIKAA CQCVEPIKSA VCIADPLPTP SQEKSQTELP DEKIGKKRKR KDDGENAKPI KKII GDGTR DPCLPYSWIS PTTGIIISDL TMEMNRFRLI GPLSHSILTE AIKAASVHTV GEDTEETPHR WWIETCKKPD SVSLH CRQE AIFELLGGIT SPAEIPAGTI LGLTVGDPRI NLPQKKSKAL PNPEKCQDNE KVRQLLLEGV PVECTHSFIW NQDICK SVT ENKISDQDLN RMRSELLVPG SQLILGPHES KIPILLIQQP GKVTGEDRLG WGSGWDVLLP KGWGMAFWIP FIYRGVR VG GLKESAVHSQ YKRSPNVPGD FPDCPAGMLF AEEQAKNLLE KYKRRPPAKR PNYVKLGTLA PFCCPWEQLT QDWESRVQ A YEEPSVASSP NGKESDLRRS EVPCAPMPKK THQPSDEVGT SIEHPREAEE VMDAGCQESA GPERITDQEA SENHVAATG SHLCVLRSRK LLKQLSAWCG PSSEDSRGGR RAPGRGQQGL TREACLSILG HFPRALVWVS LSLLSKGSPE PHTMICVPAK EDFLQLHED WHYCGPQESK HSDPFRSKIL KQKEKKKREK RQKPGRASSD GPAGEEPVAG QEALTLGLWS GPLPRVTLHC S RTLLGFVT QGDFSMAVGC GEALGFVSLT GLLDMLSSQP AAQRGLVLLR PPASLQYRFA RIAIEV

UniProtKB: Ribonucleases P/MRP protein subunit POP1

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Macromolecule #3: Ribonuclease P protein subunit p38

MacromoleculeName: Ribonuclease P protein subunit p38 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 31.891443 KDa
SequenceString: MAAAPQAPGR GSLRKTRPLV VKTSLNNPYI IRWSALESED MHFILQTLED RLKAIGLQKI EDKKKKNKTP FLKKESREKC SIAVDISEN LKEKKTDAKQ QVSGWTPAHV RKQLAIGVNE VTRALERREL LLVLVCKSVK PAMITSHLIQ LSLSRSVPAC Q VPRLSERI ...String:
MAAAPQAPGR GSLRKTRPLV VKTSLNNPYI IRWSALESED MHFILQTLED RLKAIGLQKI EDKKKKNKTP FLKKESREKC SIAVDISEN LKEKKTDAKQ QVSGWTPAHV RKQLAIGVNE VTRALERREL LLVLVCKSVK PAMITSHLIQ LSLSRSVPAC Q VPRLSERI APVIGLKCVL ALAFKKNTTD FVDEVRAIIP RVPSLSVPWL QDRIEDSGEN LETEPLESQD RELLDTSFED LS KPKRKLA DGRQASVTLQ PLKIKKLIPN PNKIRKPPKS KKATPK

UniProtKB: Ribonuclease P protein subunit p38

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Macromolecule #4: Ribonuclease P protein subunit p29

MacromoleculeName: Ribonuclease P protein subunit p29 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.474854 KDa
SequenceString: MKSVIYHALS QKEANDSDVQ PSGAQRAEAF VRAFLKRSTP RMSPQAREDQ LQRKAVVLEY FTRHKRKEKK KKAKGLSARQ RRELRLFDI KPEQQRYSLF LPLHELWKQY IRDLCSGLKP DTQPQMIQAK LLKADLHGAI ISVTKSKCPS YVGITGILLQ E TKHIFKII ...String:
MKSVIYHALS QKEANDSDVQ PSGAQRAEAF VRAFLKRSTP RMSPQAREDQ LQRKAVVLEY FTRHKRKEKK KKAKGLSARQ RRELRLFDI KPEQQRYSLF LPLHELWKQY IRDLCSGLKP DTQPQMIQAK LLKADLHGAI ISVTKSKCPS YVGITGILLQ E TKHIFKII TKEDRLKVIP KLNCVFTVET DGFISYIYGS KFQLRSSERS AKKFKAKGTI DL

UniProtKB: Ribonuclease P protein subunit p29

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Macromolecule #5: Ribonuclease P/MRP protein subunit POP5

MacromoleculeName: Ribonuclease P/MRP protein subunit POP5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 18.844672 KDa
SequenceString:
MVRFKHRYLL CELVSDDPRC RLSLDDRVLS SLVRDTIARV HGTFGAAACS IGFAVRYLNA YTGIVLLRCR KEFYQLVWSA LPFITYLEN KGHRYPCFFN TLHVGGTIRT CQKFLIQYNR RQLLILLQNC TDEGEREAIQ KSVTRSCLLE EEEESGEEAA E AME

UniProtKB: Ribonuclease P/MRP protein subunit POP5

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Macromolecule #6: Ribonuclease P protein subunit p25

MacromoleculeName: Ribonuclease P protein subunit p25 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.659406 KDa
SequenceString: MENFRKVRSE EAPAGCGAEG GGPGSGPFAD LAPGAVHMRV KEGSKIRNLM AFATASMAQP ATRAIVFSGC GRATTKTVTC AEILKRRLA GLHQVTRLRY RSVREVWQSL PPGPTQGQTP GEPAASLSVL KNVPGLAILL SKDALDPRQP GYQPPNPHPG P SSPPAAPA ...String:
MENFRKVRSE EAPAGCGAEG GGPGSGPFAD LAPGAVHMRV KEGSKIRNLM AFATASMAQP ATRAIVFSGC GRATTKTVTC AEILKRRLA GLHQVTRLRY RSVREVWQSL PPGPTQGQTP GEPAASLSVL KNVPGLAILL SKDALDPRQP GYQPPNPHPG P SSPPAAPA SKRSLGEPAA GEGSAKRSQP EPGVADEDQT A

UniProtKB: Ribonuclease P protein subunit p25

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Macromolecule #7: Ribonuclease P protein subunit p20

MacromoleculeName: Ribonuclease P protein subunit p20 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.672866 KDa
SequenceString:
MAENREPRGA VEAELDPVEY TLRKRLPSRL PRRPNDIYVN MKTDFKAQLA RCQKLLDGGA RGQNACSEIY IHGLGLAINR AINIALQLQ AGSFGSLQVA ANTSTVELVD ELEPETDTRE PLTRIRNNSA IHIRVFRVTP K

UniProtKB: Ribonuclease P protein subunit p20

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Macromolecule #8: Ribonuclease P protein subunit p14

MacromoleculeName: Ribonuclease P protein subunit p14 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.707014 KDa
SequenceString:
MPAPAATYER VVYKNPSEYH YMKVCLEFQD CGVGLNAAQF KQLLISAVKD LFGEVDAALP LDILTYEEKT LSAILRICSS GLVKLWSSL TLLGSYKGKK CAFRVIQVSP FLLALSGNSR ELVLD

UniProtKB: Ribonuclease P protein subunit p14

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Macromolecule #9: Ribonuclease P protein subunit p30

MacromoleculeName: Ribonuclease P protein subunit p30 / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.364277 KDa
SequenceString: MAVFADLDLR AGSDLKALRG LVETAAHLGY SVVAINHIVD FKEKKQEIEK PVAVSELFTT LPIVQGKSRP IKILTRLTII VSDPSHCNV LRATSSRARL YDVVAVFPKT EKLFHIACTH LDVDLVCITV TEKLPFYFKR PPINVAIDRG LAFELVYSPA I KDSTMRRY ...String:
MAVFADLDLR AGSDLKALRG LVETAAHLGY SVVAINHIVD FKEKKQEIEK PVAVSELFTT LPIVQGKSRP IKILTRLTII VSDPSHCNV LRATSSRARL YDVVAVFPKT EKLFHIACTH LDVDLVCITV TEKLPFYFKR PPINVAIDRG LAFELVYSPA I KDSTMRRY TISSALNLMQ ICKGKNVIIS SAAERPLEIR GPYDVANLGL LFGLSESDAK AAVSTNCRAA LLHGETRKTA FG IISTVKK PRPSEGDEDC LPASKKAKCE G

UniProtKB: Ribonuclease P protein subunit p30

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Macromolecule #10: UPF0711 protein C18orf21

MacromoleculeName: UPF0711 protein C18orf21 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.876859 KDa
SequenceString: MRQKHYLEAA ARGLHDSCPG QARYLLWAYT SSHDDKSTFE ETCPYCFQLL VLDNSRVRLK PKARLTPKIQ KLLNREARNY TLSFKEAKM VKKFKDSKSV LLITCKTCNR TVKHHGKSRS FVSTLKSNPA TPTSKLSLKT PERRTANPNH DMSGSKGKSP A SVFRTPTS ...String:
MRQKHYLEAA ARGLHDSCPG QARYLLWAYT SSHDDKSTFE ETCPYCFQLL VLDNSRVRLK PKARLTPKIQ KLLNREARNY TLSFKEAKM VKKFKDSKSV LLITCKTCNR TVKHHGKSRS FVSTLKSNPA TPTSKLSLKT PERRTANPNH DMSGSKGKSP A SVFRTPTS GQSVSTCSSK NTSKTKKHFS QLKMLLSQNE SQKIPKVDFR NFLSSLKGGL LK

UniProtKB: Ribonuclease MRP protein subunit p24

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Macromolecule #11: Ribonuclease P protein subunit p40

MacromoleculeName: Ribonuclease P protein subunit p40 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 41.884844 KDa
SequenceString: MATLRRLREA PRHLLVCEKS NFGNHKSRHR HLVQTHYYNY RVSFLIPECG ILSEELKNLV MNTGPYYFVK NLPLHELITP EFISTFIKK GSCYALTYNT HIDEDNTVAL LPNGKLILSL DKDTYEETGL QGHPSQFSGR KIMKFIVSID LMELSLNLDS K KYERISWS ...String:
MATLRRLREA PRHLLVCEKS NFGNHKSRHR HLVQTHYYNY RVSFLIPECG ILSEELKNLV MNTGPYYFVK NLPLHELITP EFISTFIKK GSCYALTYNT HIDEDNTVAL LPNGKLILSL DKDTYEETGL QGHPSQFSGR KIMKFIVSID LMELSLNLDS K KYERISWS FKEKKPLKFD FLLAWHKTGS EESTMMSYFS KYQIQEHQPK VALSTLRDLQ CPVLQSSELE GTPEVSCRAL EL FDWLGAV FSNVDLNNEP NNFISTYCCP EPSTVVAKAY LCTITGFILP EKICLLLEHL CHYFDEPKLA PWVTLSVQGF ADS PVSWEK NEHGFRKGGE HLYNFVIFNN QDYWLQMAVG ANDHCPP

UniProtKB: Ribonuclease P protein subunit p40

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Macromolecule #12: Nucleolus and neural progenitor protein

MacromoleculeName: Nucleolus and neural progenitor protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 64.65375 KDa
SequenceString: MMAAVPPGLE PWNRVRIPKA GNRSAVTVQN PGAALDLCIA AVIKECHLVI LSLKSQTLDA ETDVLCAVLY SNHNRMGRHK PHLALKQVE QCLKRLKNMN LEGSIQDLFE LFSSNENQPL TTKVCVVPSQ PVVELVLMKV LGACKLLLRL LDCCCKTFLL T VKHLGLQE ...String:
MMAAVPPGLE PWNRVRIPKA GNRSAVTVQN PGAALDLCIA AVIKECHLVI LSLKSQTLDA ETDVLCAVLY SNHNRMGRHK PHLALKQVE QCLKRLKNMN LEGSIQDLFE LFSSNENQPL TTKVCVVPSQ PVVELVLMKV LGACKLLLRL LDCCCKTFLL T VKHLGLQE FIILNLVMVG LVSRLWVLYK GVLKRLILLY EPLFGLLQEV ARIQPMPYFK DFTFPSDITE FLGQPYFEAF KK KMPIAFA AKGINKLLNK LFLINEQSPR ASEETLLGIS KKAKQMKINV QNNVDLGQPV KNKRVFKEES SEFDVRAFCN QLK HKATQE TSFDFKCSQS RLKTTKYSSQ KVIGTPHAKS FVQRFREAES FTQLSEEIQM AVVWCRSKKL KAQAIFLGNK LLKS NRLKH LEAQGTSLPK KLECIKTSIC NHLLRGSGIK TSKHHLRQRR SQNKFLRRQR KPQRKLQSTL LREIQQFSQG TRKSA TDTS AKWRLSHCTV HRTDLYPNSK QLLNSGVSMP VIQTKEKMIH ENLRGIHENE TDSWTVMQIN KNSTSGTIKE TDDIDD IFA LMGV

UniProtKB: Ribonuclease MRP subunit P64

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Macromolecule #13: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 13 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.93 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 294518
Initial angle assignmentType: COMMON LINE
Final angle assignmentType: MAXIMUM LIKELIHOOD

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