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- EMDB-63568: CFAP77-KO Mouse Sperm Axoneme DMT -

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Basic information

Entry
Database: EMDB / ID: EMD-63568
TitleCFAP77-KO Mouse Sperm Axoneme DMT
Map data
Sample
  • Cell: CFAP77-KO DMT of mouse sperm
KeywordsDMT / Axoneme / Sperm / UNKNOWN FUNCTION
Biological speciesMus (mice)
Methodsubtomogram averaging / cryo EM / Resolution: 24.0 Å
AuthorsSun F / Zhu Y / Yin G
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: PLoS Biol / Year: 2025
Title: The core outer junction protein CFAP77 connects A- and B-tubules within doublet microtubules of cilia and flagella.
Authors: Lan Xia / Guo-Liang Yin / Yu Long / Fei Sun / Bin-Bin Wang / Yun Zhu / Su-Ren Chen /
Abstract: The assembly and physiological function of cilia and flagella depend on the stable association of A- and B-tubules, which form axonemal doublet microtubules (DMTs). However, the mechanisms underlying ...The assembly and physiological function of cilia and flagella depend on the stable association of A- and B-tubules, which form axonemal doublet microtubules (DMTs). However, the mechanisms underlying the connection of B-tubules to A-tubules to form DMTs in mammalian cilia/flagella are unclear. CFAP77 encodes an outer junction (OJ) protein within DMTs that is conserved across many species and cell types. In this study, Cfap77-KO mice were generated to reveal that CFAP77 is essential for sperm progressive motility and male fertility. Loss of CFAP77 led to opened B-tubules specifically at the OJ regions of axonemal DMTs as revealed by conventional transmission electron microscopy. Cryo-electron tomography was used to further resolve the in situ structure of sperm axonemal DMTs directly from Cfap77-KO mice, which exhibited a loss of large filamentous density corresponding to the CFAP77-CCDC105-TEX43 ternary subcomplex at the OJ regions. Additionally, sperm proteomic analysis confirmed that CFAP77 knockout led to the complete loss of this ternary complex. Our work not only explores the physiological role of the OJ protein CFAP77 in axonemal A- and B-tubule connections in mammals but also combines in situ structural biology and knockout mice to reveal the underlying structural/molecular mechanism involved.
History
DepositionFeb 25, 2025-
Header (metadata) releaseOct 15, 2025-
Map releaseOct 15, 2025-
UpdateNov 5, 2025-
Current statusNov 5, 2025Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_63568.png

Downloads & links

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Map

FileDownload / File: emd_63568.map.gz / Format: CCP4 / Size: 8.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
6.8 Å/pix.
x 132 pix.
= 897.6 Å		6.8 Å/pix.
x 132 pix.
= 897.6 Å		6.8 Å/pix.
x 132 pix.
= 897.6 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 6.8 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 1.0
Minimum - Maximum-0.51301664 - 1.2028642
Average (Standard dev.)0.026529472 (±0.24988255)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions132132132
Spacing132132132
CellA=B=C: 897.60004 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_63568_half_map_1.map
Projections & Slices
AxesZYX

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Histogram

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Half map: #2

Fileemd_63568_half_map_2.map
Projections & Slices
AxesZYX

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Histogram

Histogram (log scale)

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Sample components

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Entire : CFAP77-KO DMT of mouse sperm

EntireName: CFAP77-KO DMT of mouse sperm
Components
  • Cell: CFAP77-KO DMT of mouse sperm

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Supramolecule #1: CFAP77-KO DMT of mouse sperm

SupramoleculeName: CFAP77-KO DMT of mouse sperm / type: cell / ID: 1 / Parent: 0
Source (natural)Organism: Mus (mice)

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statecell

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: OTHER

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 3.5 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: OTHER
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionResolution.type: BY AUTHOR / Resolution: 24.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 10000
ExtractionNumber tomograms: 100 / Number images used: 66000
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Final angle assignmentType: OTHER

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