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Yorodumi- EMDB-63567: Cryo-EM consensus structure of the SPATA5-SPATA5L1-CINP-C1orf109 ... -
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Open data
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Basic information
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| Title | Cryo-EM consensus structure of the SPATA5-SPATA5L1-CINP-C1orf109 complex | |||||||||
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Keywords | AAA+ ATPase / ribosome biogenesis / SPATA5 / CYTOSOLIC PROTEIN | |||||||||
| Biological species | Homo sapiens (human) | |||||||||
| Method | single particle reconstruction / cryo EM / Resolution: 3.8 Å | |||||||||
Authors | Dai Y / Zhang Y / Gao N | |||||||||
| Funding support | China, 1 items
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Citation | Journal: To Be PublishedTitle: Cryo-EM consensus structure of the SPATA5-SPATA5L1-CINP-C1orf109 complex Authors: Dai Y / Zhang Y / Gao N | |||||||||
| History |
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Structure visualization
| Supplemental images |
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Downloads & links
-EMDB archive
| Map data | emd_63567.map.gz | 41.7 MB | EMDB map data format | |
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| Header (meta data) | emd-63567-v30.xml emd-63567.xml | 11.9 KB 11.9 KB | Display Display | EMDB header |
| Images | emd_63567.png | 58.2 KB | ||
| Filedesc metadata | emd-63567.cif.gz | 3.8 KB | ||
| Others | emd_63567_half_map_1.map.gz emd_63567_half_map_2.map.gz | 77.8 MB 77.8 MB | ||
| Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-63567 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-63567 | HTTPS FTP |
-Validation report
| Summary document | emd_63567_validation.pdf.gz | 847.5 KB | Display | EMDB validaton report |
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| Full document | emd_63567_full_validation.pdf.gz | 847 KB | Display | |
| Data in XML | emd_63567_validation.xml.gz | 12.9 KB | Display | |
| Data in CIF | emd_63567_validation.cif.gz | 15.4 KB | Display | |
| Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-63567 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-63567 | HTTPS FTP |
-Related structure data
| Related structure data |
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Links
| EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Map
| File | Download / File: emd_63567.map.gz / Format: CCP4 / Size: 83.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
| Voxel size | X=Y=Z: 1.092 Å | ||||||||||||||||||||||||||||||||||||
| Density |
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| Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
| Details | EMDB XML:
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-Supplemental data
-Half map: #1
| File | emd_63567_half_map_1.map | ||||||||||||
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| Density Histograms |
-Half map: #2
| File | emd_63567_half_map_2.map | ||||||||||||
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| Density Histograms |
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Sample components
-Entire : The SPATA5 complex consensus structure
| Entire | Name: The SPATA5 complex consensus structure |
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| Components |
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-Supramolecule #1: The SPATA5 complex consensus structure
| Supramolecule | Name: The SPATA5 complex consensus structure / type: complex / ID: 1 / Parent: 0 |
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| Source (natural) | Organism: Homo sapiens (human) |
-Experimental details
-Structure determination
| Method | cryo EM |
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Processing | single particle reconstruction |
| Aggregation state | particle |
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Sample preparation
| Buffer | pH: 7.5 |
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| Vitrification | Cryogen name: ETHANE |
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Electron microscopy
| Microscope | TFS KRIOS |
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| Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
| Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
| Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.2 µm |
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
| Startup model | Type of model: INSILICO MODEL |
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| Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 194789 |
| Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
| Final angle assignment | Type: MAXIMUM LIKELIHOOD |
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About Yorodumi



Keywords
Homo sapiens (human)
Authors
China, 1 items
Citation
Z (Sec.)
Y (Row.)
X (Col.)




































FIELD EMISSION GUN
