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- EMDB-63487: CryoEM map of recombinant human MCM SH with MCM3 I804A/F806A/I808A -

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Open data


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Basic information

Entry
Database: EMDB / ID: EMD-63487
TitleCryoEM map of recombinant human MCM SH with MCM3 I804A/F806A/I808A
Map data
Sample
  • Complex: Mini-chromosome maintenance proteins 2-7 (MCM2-7)
KeywordsMCM / Helicase / DNA replication / CELL CYCLE
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.45 Å
AuthorsYang M / Lu P / Liu Y / Gao H / Yu H
Funding support China, 2 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32271258 China
National Natural Science Foundation of China (NSFC)3213005 China
CitationJournal: To Be Published
Title: Structures of recombinant human MCM
Authors: Liu Y / Gao H / Yu H
History
DepositionFeb 19, 2025-
Header (metadata) releaseFeb 18, 2026-
Map releaseFeb 18, 2026-
UpdateFeb 18, 2026-
Current statusFeb 18, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_63487.png

Downloads & links

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Map

FileDownload / File: emd_63487.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.08 Å/pix.
x 400 pix.
= 430.92 Å		1.08 Å/pix.
x 400 pix.
= 430.92 Å		1.08 Å/pix.
x 400 pix.
= 430.92 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.0773 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0675
Minimum - Maximum-1.6015445 - 2.824788
Average (Standard dev.)-0.0018580938 (±0.037287552)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 430.91998 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_63487_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_63487_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Mini-chromosome maintenance proteins 2-7 (MCM2-7)

EntireName: Mini-chromosome maintenance proteins 2-7 (MCM2-7)
Components
  • Complex: Mini-chromosome maintenance proteins 2-7 (MCM2-7)

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Supramolecule #1: Mini-chromosome maintenance proteins 2-7 (MCM2-7)

SupramoleculeName: Mini-chromosome maintenance proteins 2-7 (MCM2-7) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#7
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.45 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 111323
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

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