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- EMDB-62518: Enterovirus B 75 -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-62518
TitleEnterovirus B 75
Map data
Sample
  • Virus: Enterovirus B75
    • Protein or peptide: Enterovirus B 75 chain A
    • Protein or peptide: Enterovirus B 75 chain B
    • Protein or peptide: Enterovirus B 75 chain C
KeywordsEnterovirus B 75 / VIRAL PROTEIN
Biological speciesEnterovirus B75
Methodsingle particle reconstruction / cryo EM / Resolution: 2.29 Å
AuthorsTang L / Cao S / Chen Q / Zhong C / Wang F / Liu Z / Zhang X / Ke C
Funding support2 items
OrganizationGrant numberCountry
Other government2023B1212010010
Other governmentC10120180025
CitationJournal: To Be Published
Title: Enterovirus B 75
Authors: Tang L / Cao S / Chen Q / Zhong C / Wang F / Liu Z / Zhang X / Ke C
History
DepositionNov 27, 2024-
Header (metadata) releaseDec 24, 2025-
Map releaseDec 24, 2025-
UpdateDec 24, 2025-
Current statusDec 24, 2025Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_62518.png

Downloads & links

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Map

FileDownload / File: emd_62518.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.06 Å/pix.
x 480 pix.
= 508.8 Å		1.06 Å/pix.
x 480 pix.
= 508.8 Å		1.06 Å/pix.
x 480 pix.
= 508.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.06 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.12
Minimum - Maximum-2.110893 - 3.7674022
Average (Standard dev.)-0.0042706993 (±0.18908335)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 508.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_62518_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_62518_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Enterovirus B75

EntireName: Enterovirus B75
Components
  • Virus: Enterovirus B75
    • Protein or peptide: Enterovirus B 75 chain A
    • Protein or peptide: Enterovirus B 75 chain B
    • Protein or peptide: Enterovirus B 75 chain C

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Supramolecule #1: Enterovirus B75

SupramoleculeName: Enterovirus B75 / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 282345 / Sci species name: Enterovirus B75 / Virus type: VIRION / Virus isolate: SPECIES / Virus enveloped: No / Virus empty: No

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Macromolecule #1: Enterovirus B 75 chain A

MacromoleculeName: Enterovirus B 75 chain A / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterovirus B75
Molecular weightTheoretical: 26.105463 KDa
SequenceString: FVGGFHTRSE SSLENFLGRA ANTYRTSFKT LSTKPSLKFA SWTITTRKVA MLRRKMEMFT YMRFDVEVTF VITSSKCKSD PSSGSLPVL TFMVQYVPPG GPVPSSVDSF SWQSSTNPSI FWTQGNAPPR MSIPYMSIGN AYSNYYDGYS HHGADGVYGY N TLNNQGQL ...String:
FVGGFHTRSE SSLENFLGRA ANTYRTSFKT LSTKPSLKFA SWTITTRKVA MLRRKMEMFT YMRFDVEVTF VITSSKCKSD PSSGSLPVL TFMVQYVPPG GPVPSSVDSF SWQSSTNPSI FWTQGNAPPR MSIPYMSIGN AYSNYYDGYS HHGADGVYGY N TLNNQGQL YFRHVNSSNP GAYTCVVKIY FKLQHVKAWV PRPPRLCNYQ RAENVNFKVQ GVTDTKESIT ANPG

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Macromolecule #2: Enterovirus B 75 chain B

MacromoleculeName: Enterovirus B 75 chain B / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterovirus B75
Molecular weightTheoretical: 27.50524 KDa
SequenceString: SSNVRSITLG NSTITTQESS NVVVGFGVWP SYLSKSKSGS GSTQPTQPDT ATERFYTLES VKWQKTDNGW YWKFPDALKD MGLFGQNML YHFLGRTGWT IHVKCEASRF FQGALLVVCV PQAEKGCAVI TRDVNATALD EGDKAKKFTA GSVSSQNGVQ T AVYNAAMG ...String:
SSNVRSITLG NSTITTQESS NVVVGFGVWP SYLSKSKSGS GSTQPTQPDT ATERFYTLES VKWQKTDNGW YWKFPDALKD MGLFGQNML YHFLGRTGWT IHVKCEASRF FQGALLVVCV PQAEKGCAVI TRDVNATALD EGDKAKKFTA GSVSSQNGVQ T AVYNAAMG VAVGNLTIFP HQWINLKTNN SATIVKPYIN SIPMENMYRH FNFTLMVIPF VPLEWSVGAC TYVPITVTVA PQ NAEYIGL RLA

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Macromolecule #3: Enterovirus B 75 chain C

MacromoleculeName: Enterovirus B 75 chain C / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterovirus B75
Molecular weightTheoretical: 26.301756 KDa
SequenceString: GNPTQLVPGS NMYLTSDDFR SPEAMPQFEV TPEMFIPGEV HNLMEIAETD STVPVNNVGM AVNSMEAYRI GVNPNTGSGT QVYGWPLNP GKSSVFKRTN LGEILNYFAH WSGSIQLTFV FCGSAQATGK FLLAYSPPGA GAPTDRKGAR LGTHVVWDVG L QSSCVLEV ...String:
GNPTQLVPGS NMYLTSDDFR SPEAMPQFEV TPEMFIPGEV HNLMEIAETD STVPVNNVGM AVNSMEAYRI GVNPNTGSGT QVYGWPLNP GKSSVFKRTN LGEILNYFAH WSGSIQLTFV FCGSAQATGK FLLAYSPPGA GAPTDRKGAR LGTHVVWDVG L QSSCVLEV PWISNTFYRK DKSSGTGFSK AGYVTCWFQT SIVAPADAMS KCYIMCFVSA DNDFSVRLLK DTPFIKEQAA RR V

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 45.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.0 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 81000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.29 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 22476
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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