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- EMDB-62207: Cryo-EM structure of the SPS3-FBN5 complex in a 2:1 state -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-62207
TitleCryo-EM structure of the SPS3-FBN5 complex in a 2:1 state
Map data
Sample
  • Complex: Cryo-EM structure of the SPS3-FBN5 complex in a 2:1 state
    • Protein or peptide: Fibrillin protein 5 homolog
    • Protein or peptide: Probable solanesyl-diphosphate synthase 3, chloroplastic
Keywordschloroplast / TRANSFERASE
Function / homology
Function and homology information


all-trans-nonaprenyl diphosphate synthase [geranyl-diphosphate specific] / all-trans-nonaprenyl-diphosphate synthase (geranyl-diphosphate specific) activity / plastoquinone biosynthetic process / prenyltransferase activity / isoprenoid biosynthetic process / chloroplast thylakoid membrane / chloroplast / metal ion binding
Similarity search - Function
Plastid lipid-associated protein/fibrillin conserved domain / Plastid-lipid-associated protein / PAP_fibrillin / Polyprenyl synthases signature 1. / Polyprenyl synthases signature 2. / Polyprenyl synthetase, conserved site / Polyprenyl synthetase / Polyprenyl synthetase / Isoprenoid synthase domain superfamily
Similarity search - Domain/homology
Probable solanesyl-diphosphate synthase 3, chloroplastic / Fibrillin protein 5 homolog
Similarity search - Component
Biological speciesOryza sativa subsp. japonica (Japanese rice)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.9 Å
AuthorsXiao H / Wang Y-W / Zhu P / Yang G-F
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: To Be Published
Title: Cryo-EM structure of the SPS3-FBN5 complex in a 2:1 state
Authors: Xiao H / Wang Y-W / Zhu P / Yang G-F
History
DepositionOct 28, 2024-
Header (metadata) releaseMay 6, 2026-
Map releaseMay 6, 2026-
UpdateMay 6, 2026-
Current statusMay 6, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_62207.png

Downloads & links

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Map

FileDownload / File: emd_62207.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.75 Å/pix.
x 200 pix.
= 150. Å		0.75 Å/pix.
x 200 pix.
= 150. Å		0.75 Å/pix.
x 200 pix.
= 150. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.75 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 4.0
Minimum - Maximum-0.12132219 - 11.972382
Average (Standard dev.)0.059887048 (±0.7451151)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions200200200
Spacing200200200
CellA=B=C: 150.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_62207_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_62207_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Cryo-EM structure of the SPS3-FBN5 complex in a 2:1 state

EntireName: Cryo-EM structure of the SPS3-FBN5 complex in a 2:1 state
Components
  • Complex: Cryo-EM structure of the SPS3-FBN5 complex in a 2:1 state
    • Protein or peptide: Fibrillin protein 5 homolog
    • Protein or peptide: Probable solanesyl-diphosphate synthase 3, chloroplastic

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Supramolecule #1: Cryo-EM structure of the SPS3-FBN5 complex in a 2:1 state

SupramoleculeName: Cryo-EM structure of the SPS3-FBN5 complex in a 2:1 state
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Oryza sativa subsp. japonica (Japanese rice)

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Macromolecule #1: Fibrillin protein 5 homolog

MacromoleculeName: Fibrillin protein 5 homolog / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryza sativa subsp. japonica (Japanese rice)
Molecular weightTheoretical: 22.99935 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: QARAVGSYEA ALGDAKDALY AALEGMNRGI FGMTSEKRSE IHALVELLES KNPTPEPTDK LQDKVDGCWR LVYSTISILG KKRTKLGLR DFISLGDFFQ MIDVKEEKAV NVIKFSARAL KILSGQLTIE ASYKITTKTK VDITLDSSTI TPDQLMNIFQ K NYDMLLAI ...String:
QARAVGSYEA ALGDAKDALY AALEGMNRGI FGMTSEKRSE IHALVELLES KNPTPEPTDK LQDKVDGCWR LVYSTISILG KKRTKLGLR DFISLGDFFQ MIDVKEEKAV NVIKFSARAL KILSGQLTIE ASYKITTKTK VDITLDSSTI TPDQLMNIFQ K NYDMLLAI FNPEGWLEIT YVDESLRIGR DDKANIFVLE RADPSEV

UniProtKB: Fibrillin protein 5 homolog

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Macromolecule #2: Probable solanesyl-diphosphate synthase 3, chloroplastic

MacromoleculeName: Probable solanesyl-diphosphate synthase 3, chloroplastic
type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
EC number: all-trans-nonaprenyl diphosphate synthase [geranyl-diphosphate specific]
Source (natural)Organism: Oryza sativa subsp. japonica (Japanese rice)
Molecular weightTheoretical: 35.860766 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: RISVSSLLEV VADDLLKLNN NLKSLVGAEN PVLVSAAEQI FGAGGKRLRP ALVFLVSRAT AELAGLLELT TEHQRLAEII EMIHTASLI HDDVIDDSGM RRGKETIHQL YGTRVAVLAG DFMFAQSSWF LANLENIEVI KLISQVIKDF ASGEIKQAST L FDCDITLD ...String:
RISVSSLLEV VADDLLKLNN NLKSLVGAEN PVLVSAAEQI FGAGGKRLRP ALVFLVSRAT AELAGLLELT TEHQRLAEII EMIHTASLI HDDVIDDSGM RRGKETIHQL YGTRVAVLAG DFMFAQSSWF LANLENIEVI KLISQVIKDF ASGEIKQAST L FDCDITLD DYLLKSYYKT ASLIAASTRS AAIFSGVSTA ICEQMYEYGR NLGLSFQVVD DILDFTQSAE QLGKPAGSDL AK GNLTAPV IFALQDEPQL REIIDSEFSE TNSLATAIEL VHRSGGIKRA HELAREKGEI AIQSLQCLPR SEFRSTLENM VKY NLERID

UniProtKB: Probable solanesyl-diphosphate synthase 3, chloroplastic

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 49.43 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.4000000000000001 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 530909
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING
FSC plot (resolution estimation)

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