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- EMDB-62150: Cryo-EM structure of human taurine transporter TAUT bound taurine... -

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Basic information

Entry
Database: EMDB / ID: EMD-62150
TitleCryo-EM structure of human taurine transporter TAUT bound taurine in an occluded state in the saposin-disc
Map data
Sample
  • Complex: Sodium- and chloride-dependent taurine transporter
    • Protein or peptide: Sodium- and chloride-dependent taurine transporter
  • Ligand: 2-AMINOETHANESULFONIC ACID
  • Ligand: CHLORIDE ION
KeywordsComplex / TRANSPORT PROTEIN
Function / homology
Function and homology information


taurine transmembrane transporter activity / alanine transmembrane transporter activity / gamma-aminobutyric acid transmembrane transporter activity / taurine:sodium symporter activity / import across plasma membrane / gamma-aminobutyric acid import / gamma-aminobutyric acid:sodium:chloride symporter activity / alanine transport / amino acid:sodium symporter activity / amino acid import across plasma membrane ...taurine transmembrane transporter activity / alanine transmembrane transporter activity / gamma-aminobutyric acid transmembrane transporter activity / taurine:sodium symporter activity / import across plasma membrane / gamma-aminobutyric acid import / gamma-aminobutyric acid:sodium:chloride symporter activity / alanine transport / amino acid:sodium symporter activity / amino acid import across plasma membrane / taurine transmembrane transport / Amino acid transport across the plasma membrane / amino acid transmembrane transporter activity / SLC-mediated transport of neurotransmitters / neurotransmitter transport / microvillus membrane / amino acid transport / transport across blood-brain barrier / sodium ion transmembrane transport / cell projection / positive regulation of cell differentiation / modulation of chemical synaptic transmission / GABA-ergic synapse / basolateral plasma membrane / postsynaptic membrane / apical plasma membrane / neuronal cell body / dendrite / membrane / plasma membrane
Similarity search - Function
Sodium:neurotransmitter symporter, taurine / Sodium:neurotransmitter symporter family signature 2. / Sodium:neurotransmitter symporter family signature 1. / Sodium:neurotransmitter symporter / Sodium:neurotransmitter symporter superfamily / Sodium:neurotransmitter symporter family / Sodium:neurotransmitter symporter family profile.
Similarity search - Domain/homology
Sodium- and chloride-dependent taurine transporter
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.3 Å
AuthorsZhang H / Xu EH
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: Cell Res. / Year: 2025
Title: Cryo-EM structure of human taurine transporter TAUT bound taurine in an occluded state in the saposin-disc
Authors: Zhang H / Xu EH
History
DepositionOct 24, 2024-
Header (metadata) releaseFeb 26, 2025-
Map releaseFeb 26, 2025-
UpdateFeb 26, 2025-
Current statusFeb 26, 2025Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_62150.png

Downloads & links

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Map

FileDownload / File: emd_62150.map.gz / Format: CCP4 / Size: 91.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.73 Å/pix.
x 288 pix.
= 210.24 Å		0.73 Å/pix.
x 288 pix.
= 210.24 Å		0.73 Å/pix.
x 288 pix.
= 210.24 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.73 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.18
Minimum - Maximum-1.3881593 - 2.1945617
Average (Standard dev.)0.0011831026 (±0.053256277)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions288288288
Spacing288288288
CellA=B=C: 210.24 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_62150_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_62150_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Sodium- and chloride-dependent taurine transporter

EntireName: Sodium- and chloride-dependent taurine transporter
Components
  • Complex: Sodium- and chloride-dependent taurine transporter
    • Protein or peptide: Sodium- and chloride-dependent taurine transporter
  • Ligand: 2-AMINOETHANESULFONIC ACID
  • Ligand: CHLORIDE ION

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Supramolecule #1: Sodium- and chloride-dependent taurine transporter

SupramoleculeName: Sodium- and chloride-dependent taurine transporter / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Sodium- and chloride-dependent taurine transporter

MacromoleculeName: Sodium- and chloride-dependent taurine transporter / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 71.211 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MDYKDDDDKG SGMATKEKLQ CLKDFHKDIL KPSPGKSPGT RPEDEAEGKP PQREKWSSKI DFVLSVAGGF VGLGNVWRFP YLCYKNGGG AFLIPYFIFL FGSGLPVFFL EIIIGQYTSE GGITCWEKIC PLFSGIGYAS VVIVSLLNVY YIVILAWATY Y LFQSFQKE ...String:
MDYKDDDDKG SGMATKEKLQ CLKDFHKDIL KPSPGKSPGT RPEDEAEGKP PQREKWSSKI DFVLSVAGGF VGLGNVWRFP YLCYKNGGG AFLIPYFIFL FGSGLPVFFL EIIIGQYTSE GGITCWEKIC PLFSGIGYAS VVIVSLLNVY YIVILAWATY Y LFQSFQKE LPWAHCNHSW NTPHCMEDTM RKNKSVWITI SSTNFTSPVI EFWERNVLSL SPGIDHPGSL KWDLALCLLL VW LVCFFCI WKGVRSTGKV VYFTATFPFA MLLVLLVRGL TLPGAGAGIK FYLYPDITRL EDPQVWIDAG TQIFFSYAIC LGA MTSLGS YNKYKYNSYR DCMLLGCLNS GTSFVSGFAI FSILGFMAQE QGVDIADVAE SGPGLAFIAY PKAVTMMPLP TFWS ILFFI MLLLLGLDSQ FVEVEGQITS LVDLYPSFLR KGYRREIFIA FVCSISYLLG LTMVTEGGMY VFQLFDYYAA SGVCL LWVA FFECFVIAWI YGGDNLYDGI EDMIGYRPGP WMKYSWAVIT PVLCVGCFIF SLVKYVPLTY NKTYVYPNWA IGLGWS LAL SSMLCVPLVI VIRLCQTEGP FLVRVKYLLT PREPNRWAVE REGATPYNSR TVMNGALVKP THIIVETMM

UniProtKB: Sodium- and chloride-dependent taurine transporter

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Macromolecule #2: 2-AMINOETHANESULFONIC ACID

MacromoleculeName: 2-AMINOETHANESULFONIC ACID / type: ligand / ID: 2 / Number of copies: 1 / Formula: TAU
Molecular weightTheoretical: 125.147 Da
Chemical component information

ChemComp-TAU:
2-AMINOETHANESULFONIC ACID

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Macromolecule #3: CHLORIDE ION

MacromoleculeName: CHLORIDE ION / type: ligand / ID: 3 / Number of copies: 1 / Formula: CL
Molecular weightTheoretical: 35.453 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 50 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: DIFFRACTION / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 99965
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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