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- EMDB-61518: CryoEM structure of human GLRA3 at 1.9 angstrom resolution from B... -

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Basic information

Entry
Database: EMDB / ID: EMD-61518
TitleCryoEM structure of human GLRA3 at 1.9 angstrom resolution from Biortus
Map data
Sample
  • Organelle or cellular component: CryoEM structure of human GLRA3 at 1.9 angstrom resolution from Biortus
KeywordsGlycine receptor subunit alpha-3 / MEMBRANE PROTEIN
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 1.94 Å
AuthorsWang F / Yang Y / Shi H / Xu L / He W / Han T / Hu Y
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: CryoEM structure of human GLRA3 at 1.9 angstrom resolution from Biortus
Authors: Wang F / Yang Y / Shi H / Xu L / He W / Han T / Hu Y
History
DepositionSep 13, 2024-
Header (metadata) releaseOct 2, 2024-
Map releaseOct 2, 2024-
UpdateOct 2, 2024-
Current statusOct 2, 2024Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_61518.png

Downloads & links

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Map

FileDownload / File: emd_61518.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.58 Å/pix.
x 512 pix.
= 296.96 Å		0.58 Å/pix.
x 512 pix.
= 296.96 Å		0.58 Å/pix.
x 512 pix.
= 296.96 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.58 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.28
Minimum - Maximum-0.65168864 - 1.2219322
Average (Standard dev.)0.00009585167 (±0.027460136)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 296.96 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_61518_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_61518_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_61518_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : CryoEM structure of human GLRA3 at 1.9 angstrom resolution from B...

EntireName: CryoEM structure of human GLRA3 at 1.9 angstrom resolution from Biortus
Components
  • Organelle or cellular component: CryoEM structure of human GLRA3 at 1.9 angstrom resolution from Biortus

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Supramolecule #1: CryoEM structure of human GLRA3 at 1.9 angstrom resolution from B...

SupramoleculeName: CryoEM structure of human GLRA3 at 1.9 angstrom resolution from Biortus
type: organelle_or_cellular_component / ID: 1 / Parent: 0
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: NITROGEN

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 1.94 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 156542
Initial angle assignmentType: OTHER
Final angle assignmentType: OTHER
FSC plot (resolution estimation)

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