EMDB-61076: KU13-bond Mycobacterium tuberculosis 70S ribosome

Basic information

Entry

Database: EMDB / ID: EMD-61076

• Title: KU13-bond Mycobacterium tuberculosis 70S ribosome

Sample

• Complex: KU13-bond Mycobacterium tuberculosis 70S ribosome
  • Protein or peptide: x 48 types
  • RNA: x 4 types
• Ligand: x 3 types

• Keywords: ribosome / macrolide / ANTIBIOTIC / RIBOSOME-ANTIBIOTIC complex

Function / homology

Function:

large ribosomal subunit / transferase activity / ribosomal small subunit assembly / ribosomal small subunit biogenesis / small ribosomal subunit / small ribosomal subunit rRNA binding / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / RNA binding / zinc ion binding / cytosol / cytoplasm

Domain/homology:

Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, type Z / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / : / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / : / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / : / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / : / Ribosomal protein L6, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S14/S29 / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S4, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L33 superfamily / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L30, bacterial-type / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / : / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L20 / Ribosomal protein S12, bacterial-type / Ribosomal L32p protein family / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal

Component:

Large ribosomal subunit protein bL34 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein bL25 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein uL13 / : / : / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein bS18A / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL33A / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL20 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL27 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein bL17 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein bL36

• Biological species: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria)
• Method: single particle reconstruction / cryo EM / Resolution: 2.33 Å
• Authors: Nishihara D / Fujino M / Tanaka Y / Yokoyama T

Funding support

Japan, 1 items

Organization	Grant number	Country
Other private		Japan

• Citation: Journal: Sci Adv / Year: 2025; Title: Creation of a macrolide antibiotic against non-tuberculous using late-stage boron-mediated aglycon delivery.; Authors: Yuka Isozaki / Takumi Makikawa / Kosuke Kimura / Daiki Nishihara / Maho Fujino / Yoshikazu Tanaka / Chigusa Hayashi / Yoshimasa Ishizaki / Masayuki Igarashi / Takeshi Yokoyama / Kazunobu Toshima / Daisuke Takahashi / ; Abstract: Non-tuberculous mycobacteria (NTM) is gaining clinical recognition as a recently emerging pulmonary pathogen. complex (MAC), the most common NTM, is the cause of pulmonary MAC disease. Currently, the macrolide azithromycin (AZM) is the standard first-line antibiotic for treatment of the disease. However, the rise of drug-resistant MAC necessitates the development of alternative therapeutics. Here, we present a late-stage boron-mediated aglycon delivery strategy for selective modification of AZM, generating a library of potential anti-MAC drugs designated to . Screening of to revealed that exhibited enhanced antimicrobial activity against wild-type and macrolide-resistant MAC compared to AZM. Cryo-electron microscopy analysis indicated that the inserted tercyclic moiety of formed a robust anchor on the bacterial ribosome, creating a binding pocket with base flipping of U2847, potentially bypassing the standard mechanism of macrolide resistance. These results position as a promising lead for therapeutics against macrolide-resistant MAC.

History

Deposition	Aug 5, 2024	-
Header (metadata) release	Mar 19, 2025	-
Map release	Mar 19, 2025	-
Update	Mar 19, 2025	-
Current status	Mar 19, 2025	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_61076.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 0.788 Å

Density

Contour Level	By AUTHOR: 0.0085
Minimum - Maximum	-0.021826182 - 0.08317836
Average (Standard dev.)	0.00013378965 (±0.0025128794)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 512 512 512 Spacing 512 512 512
Cell	A=B=C: 403.456 Å α=β=γ: 90.0 °

Supplemental data

Half map: #2

• File: emd_61076_half_map_1.map

Half map: #1

• File: emd_61076_half_map_2.map

Sample components

Entire : KU13-bond Mycobacterium tuberculosis 70S ribosome

• Entire: Name: KU13-bond Mycobacterium tuberculosis 70S ribosome

Components

• Complex: KU13-bond Mycobacterium tuberculosis 70S ribosome
  • Protein or peptide: Large ribosomal subunit protein bL32
  • Protein or peptide: Large ribosomal subunit protein bL33A
  • Protein or peptide: Large ribosomal subunit protein bL34
  • Protein or peptide: Large ribosomal subunit protein bL35
  • Protein or peptide: Large ribosomal subunit protein bL36
  • Protein or peptide: 30S ribosomal protein THX
  • RNA: 23S rRNA
  • RNA: 5S rRNA
  • Protein or peptide: Large ribosomal subunit protein uL2
  • Protein or peptide: Large ribosomal subunit protein uL3
  • Protein or peptide: Large ribosomal subunit protein uL4
  • Protein or peptide: Large ribosomal subunit protein uL5
  • Protein or peptide: Large ribosomal subunit protein uL6
  • Protein or peptide: Large ribosomal subunit protein bL9
  • Protein or peptide: Large ribosomal subunit protein uL13
  • Protein or peptide: Large ribosomal subunit protein uL14
  • Protein or peptide: Large ribosomal subunit protein uL15
  • Protein or peptide: Large ribosomal subunit protein uL16
  • Protein or peptide: Large ribosomal subunit protein bL17
  • Protein or peptide: Large ribosomal subunit protein uL18
  • Protein or peptide: Large ribosomal subunit protein bL19
  • Protein or peptide: Large ribosomal subunit protein bL20
  • Protein or peptide: Large ribosomal subunit protein bL21
  • Protein or peptide: Large ribosomal subunit protein uL22
  • Protein or peptide: Large ribosomal subunit protein uL23
  • Protein or peptide: Large ribosomal subunit protein uL24
  • Protein or peptide: Large ribosomal subunit protein bL25
  • Protein or peptide: Large ribosomal subunit protein bL27
  • Protein or peptide: Large ribosomal subunit protein bL28
  • Protein or peptide: Large ribosomal subunit protein uL29
  • Protein or peptide: Large ribosomal subunit protein uL30
  • RNA: 16S rRNA
  • Protein or peptide: Small ribosomal subunit protein uS3
  • Protein or peptide: Small ribosomal subunit protein uS4
  • Protein or peptide: Small ribosomal subunit protein uS5
  • Protein or peptide: Small ribosomal subunit protein bS6
  • Protein or peptide: Small ribosomal subunit protein uS7
  • Protein or peptide: Small ribosomal subunit protein uS8
  • Protein or peptide: Small ribosomal subunit protein uS9
  • Protein or peptide: Small ribosomal subunit protein uS10
  • Protein or peptide: Small ribosomal subunit protein uS11
  • Protein or peptide: Small ribosomal subunit protein uS12
  • Protein or peptide: Small ribosomal subunit protein uS13
  • Protein or peptide: Small ribosomal subunit protein uS14B
  • Protein or peptide: Small ribosomal subunit protein uS15
  • Protein or peptide: Small ribosomal subunit protein bS16
  • Protein or peptide: Small ribosomal subunit protein uS17
  • Protein or peptide: Small ribosomal subunit protein bS18A
  • Protein or peptide: Small ribosomal subunit protein uS19
  • Protein or peptide: Small ribosomal subunit protein bS20
  • Protein or peptide: Mitochondrial domain of uncharacterised function (DUF1713)
  • RNA: tRNA
• Ligand: ZINC ION
• Ligand: MAGNESIUM ION
• Ligand: (2~{R},3~{R},4~{R},5~{R},8~{R},10~{R},11~{R},12~{S},13~{S},14~{R})-11-[(2~{S},3~{R},4~{S},6~{R})-4-(dimethylamino)-6-methyl-3-oxidanyl-oxan-2-yl]oxy-2-ethyl-4-[(2~{R},3~{R},4~{R},5~{S},6~{R})-6-(hydroxymethyl)-3,4-bis(oxidanyl)-5-[[4-(4-pyridin-4-yl-1,2,3-triazol-1-yl)phenyl]methoxy]oxan-2-yl]oxy-13-[(2~{R},4~{R},5~{S},6~{S})-4-methoxy-4,6-dimethyl-5-oxidanyl-oxan-2-yl]oxy-3,5,6,8,10,12,14-heptamethyl-3,10-bis(oxidanyl)-1-oxa-6-azacyclopentadecan-15-one

Supramolecule #1: KU13-bond Mycobacterium tuberculosis 70S ribosome

• Supramolecule: Name: KU13-bond Mycobacterium tuberculosis 70S ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#52
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria)

Macromolecule #1: Large ribosomal subunit protein bL32

• Macromolecule: Name: Large ribosomal subunit protein bL32 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 6.525757 KDa

Sequence

String:

MAVPKRRKSR SNTRSRRSQW KAAKTELVGV TVAGHAHKVP RRLLKAARLG LIDFDKR

UniProtKB: Large ribosomal subunit protein bL32

Macromolecule #2: Large ribosomal subunit protein bL33A

• Macromolecule: Name: Large ribosomal subunit protein bL33A / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 6.512581 KDa

Sequence

String:

MASSTDVRPK ITLACEVCKH RNYITKKNRR NDPDRLELKK FCPNCGKHQA HRETR

UniProtKB: Large ribosomal subunit protein bL33A

Macromolecule #3: Large ribosomal subunit protein bL34

• Macromolecule: Name: Large ribosomal subunit protein bL34 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 5.643697 KDa

Sequence

String:

MTKGKRTFQP NNRRRARVHG FRLRMRTRAG RSIVSSRRRK GRRTLSA

UniProtKB: Large ribosomal subunit protein bL34

Macromolecule #4: Large ribosomal subunit protein bL35

• Macromolecule: Name: Large ribosomal subunit protein bL35 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 7.24143 KDa

Sequence

String:

MPKAKTHSGA SKRFRRTGTG KIVRQKANRR HLLEHKPSTR TRRLDGRTVV AANDTKRVTS LLNG

UniProtKB: Large ribosomal subunit protein bL35

Macromolecule #5: Large ribosomal subunit protein bL36

• Macromolecule: Name: Large ribosomal subunit protein bL36 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 4.355305 KDa

Sequence

String:

MKVNPSVKPI CDKCRLIRRH GRVMVICSDP RHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

Macromolecule #6: 30S ribosomal protein THX

• Macromolecule: Name: 30S ribosomal protein THX / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 2.882402 KDa

Sequence

String:

MAKRGRKKRD RKYSKANHGK RPNS

UniProtKB: UNIPROTKB: A0AAE8WR80

Macromolecule #9: Large ribosomal subunit protein uL2

• Macromolecule: Name: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 30.644002 KDa

Sequence

String:

MAIRKYKPTT PGRRGASVSD FAEITRSTPE KSLVRPLHGR GGRNAHGRIT TRHKGGGHKR AYRMIDFRRN DKDGVNAKVA HIEYDPNRT ARIALLHYLD GEKRYIIAPN GLSQGDVVES GANADIKPGN NLPLRNIPAG TLIHAVELRP GGGAKLARSA G SSIQLLGK EASYASLRMP SGEIRRVDVR CRATVGEVGN AEQANINWGK AGRMRWKGKR PSVRGVVMNP VDHPHGGGEG KT SGGRHPV SPWGKPEGRT RNANKSSNKF IVRRRRTGKK HSR

UniProtKB: Large ribosomal subunit protein uL2

Macromolecule #10: Large ribosomal subunit protein uL3

• Macromolecule: Name: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 23.127533 KDa

Sequence

String:

MARKGILGTK LGMTQVFDES NRVVPVTVVK AGPNVVTRIR TPERDGYSAV QLAYGEISPR KVNKPLTGQY TAAGVNPRRY LAELRLDDS DAATEYQVGQ ELTAEIFADG SYVDVTGTSK GKGFAGTMKR HGFRGQGASH GAQAVHRRPG SIGGCATPAR V FKGTRMAG RMGNDRVTVL NLLVHKVDAE NGVLLIKGAV PGRTGGLVMV RSAIKRGEK

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #11: Large ribosomal subunit protein uL4

• Macromolecule: Name: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 23.779936 KDa

Sequence

String:

MAAQEQKTLK IDVKTPAGKV DGAIELPAEL FDVPANIALM HQVVTAQRAA ARQGTHSTKT RGEVSGGGRK PYRQKGTGRA RQGSTRAPQ FTGGGVVHGP KPRDYSQRTP KKMIAAALRG ALSDRARNGR IHAITELVEG QNPSTKSARA FLASLTERKQ V LVVIGRSD EAGAKSVRNL PGVHILAPDQ LNTYDVLRAD DVVFSVEALN AYIAANTTTS EEVSA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #12: Large ribosomal subunit protein uL5

• Macromolecule: Name: Large ribosomal subunit protein uL5 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 21.052303 KDa

Sequence

String:

MTTAQKVQPR LKERYRSEIR DALRKQFGYG NVMQIPTVTK VVVNMGVGEA ARDAKLINGA VNDLALITGQ KPEVRRARKS IAQFKLREG MPVGVRVTLR GDRMWEFLDR LTSIALPRIR DFRGLSPKQF DGVGNYTFGL AEQAVFHEVD VDKIDRVRGM D INVVTSAA TDDEGRALLR ALGFPFKEN

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #13: Large ribosomal subunit protein uL6

• Macromolecule: Name: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 19.408363 KDa

Sequence

String:

MSRIGKQPIP VPAGVDVTIE GQSISVKGPK GTLGLTVAEP IKVARNDDGA IVVTRPDDER RNRSLHGLSR TLVSNLVTGV TQGYTTKME IFGVGYRVQL KGSNLEFALG YSHPVVIEAP EGITFAVQAP TKFTVSGIDK QKVGQIAANI RRLRRPDPYK G KGVRYEGE QIRRKVGKTG K

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #14: Large ribosomal subunit protein bL9

• Macromolecule: Name: Large ribosomal subunit protein bL9 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 16.196654 KDa

Sequence

String:

MKLILTADVD HLGSIGDTVE VKDGYGRNFL LPRGLAIVAS RGAQKQADEI RRARETKSVR DLEHANEIKA AIEALGPIAL PVKTSADSG KLFGSVTAAD VVAAIKKAGG PNLDKRIVRL PKTHIKAVGT HFVSVHLHPE IDVEVSLDVV AQS

UniProtKB: Large ribosomal subunit protein bL9

Macromolecule #15: Large ribosomal subunit protein uL13

• Macromolecule: Name: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 21.646121 KDa

Sequence

String:

MLVGKLLGWR AVRRDLGSMS VAETNPPFTP DRRPGHPGIH PREKKVSAVP TYAPKAGDTT RSWYVIDATD VVLGRLAVAA ANLLRGKHK PTFAPNVDGG DFVIVINADK VAISGDKLQH KMVYRHSGYP GGLHKRTIGE LMQRHPDRVV EKAILGMLPK N RLSRQIQR KLRVYAGPEH PHSAQQPVPY ELKQVAQ

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #16: Large ribosomal subunit protein uL14

• Macromolecule: Name: Large ribosomal subunit protein uL14 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 13.451714 KDa

Sequence

String:

MIQQESRLKV ADNTGAKEIL CIRVLGGSSR RYAGIGDVIV ATVKDAIPGG NVKRGDVVKA VVVRTVKERR RPDGSYIKFD ENAAVIIKP DNDPRGTRIF GPVGRELREK RFMKIISLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #17: Large ribosomal subunit protein uL15

• Macromolecule: Name: Large ribosomal subunit protein uL15 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 15.554073 KDa

Sequence

String:

MTLKLHDLRP ARGSKTARTR VGRGDGSKGK TAGRGTKGTR ARKQVPVTFE GGQMPIHMRL PKLKGFRNRF RTEYEIVNVG DINRLFPQG GAVGVDDLVA KGAVRKNALV KVLGDGKLTA KVDVSAHKFS GSARAKITAA GGSATEL

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #18: Large ribosomal subunit protein uL16

• Macromolecule: Name: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 15.724277 KDa

Sequence

String:

MLIPRKVKHR KQHHPRQRGI ASGGTTVNFG DYGIQALEHA YVTNRQIESA RIAINRHIKR GGKVWINIFP DRPLTKKPAE TRMGSGKGS PEWWVANVKP GRVLFELSYP NEGVARAALT RAIHKLPIKA RIITREEQF

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #19: Large ribosomal subunit protein bL17

• Macromolecule: Name: Large ribosomal subunit protein bL17 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 19.512273 KDa

Sequence

String:

MPKPTKGPRL GGSSSHQKAI LANLATSLFE HGRITTTEPK ARALRPYAEK LITHAKKGAL HNRREVLKKL RDKDVVHTLF AEIGPFFAD RDGGYTRIIK IEARKGDNAP MAVIELVREK TVTSEANRAR RVAAAQAKAK KAAAMPTEES EAKPAEEGDV V GASEPDAK APEEPPAEAP EN

UniProtKB: Large ribosomal subunit protein bL17

Macromolecule #20: Large ribosomal subunit protein uL18

• Macromolecule: Name: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 13.211122 KDa

Sequence

String:

MAQSVSATRR ISRLRRHTRL RKKLSGTAER PRLVVHRSAR HIHVQLVNDL NGTTVAAASS IEADVRGVPG DKKARSVRVG QLIAERAKA AGIDTVVFDR GGYTYGGRIA ALADAARENG LSF

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #21: Large ribosomal subunit protein bL19

• Macromolecule: Name: Large ribosomal subunit protein bL19 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 13.041126 KDa

Sequence

String:

MNRLDFVDKP SLRDDIPAFN PGDTINVHVK VIEGAKERLQ VFKGVVIRRQ GGGIRETFTV RKESYGVGVE RTFPVHSPNI DHIEVVTRG DVRRAKLYYL RELRGKKAKI KEKR

UniProtKB: Large ribosomal subunit protein bL19

Macromolecule #22: Large ribosomal subunit protein bL20

• Macromolecule: Name: Large ribosomal subunit protein bL20 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 14.557777 KDa

Sequence

String:

MARVKRAVNA HKKRRSILKA SRGYRGQRSR LYRKAKEQQL HSLNYAYRDR RARKGEFRKL WIARINAAAR LNDITYNRLI QGLKAAGVE VDRKNLADIA ISDPAAFTAL VDVARAALPE DVNAPSGEAA

UniProtKB: Large ribosomal subunit protein bL20

Macromolecule #23: Large ribosomal subunit protein bL21

• Macromolecule: Name: Large ribosomal subunit protein bL21 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 11.173147 KDa

Sequence

String:

MMATYAIVKT GGKQYKVAVG DVVKVEKLES EQGEKVSLPV ALVVDGATVT TDAKALAKVA VTGEVLGHTK GPKIRIHKFK NKTGYHKRQ GHRQQLTVLK VTGIA

UniProtKB: Large ribosomal subunit protein bL21

Macromolecule #24: Large ribosomal subunit protein uL22

• Macromolecule: Name: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 20.424594 KDa

Sequence

String:

MTAATKATEY PSAVAKARFV RVSPRKARRV IDLVRGRSVS DALDILRWAP QAASGPVAKV IASAAANAQN NGGLDPATLV VATVYADQG PTAKRIRPRA QGRAFRIRRR TSHITVVVES RPAKDQRSAK SSRARRTEAS KAASKVGATA PAKKAAAKAP A KKAPASSG VKKTPAKKAP AKKAPAKASE TSAAKGGSD

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #25: Large ribosomal subunit protein uL23

• Macromolecule: Name: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 10.976785 KDa

Sequence

String:

MATLADPRDI ILAPVISEKS YGLLDDNVYT FLVRPDSNKT QIKIAVEKIF AVKVASVNTA NRQGKRKRTR TGYGKRKSTK RAIVTLAPG SRPIDLFGAP A

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #26: Large ribosomal subunit protein uL24

• Macromolecule: Name: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 11.500274 KDa

Sequence

String:

MKVHKGDTVL VISGKDKGAK GKVLQAYPDR NRVLVEGVNR IKKHTAISTT QRGARSGGIV TQEAPIHVSN VMVVDSDGKP TRIGYRVDE ETGKRVRISK RNGKDI

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #27: Large ribosomal subunit protein bL25

• Macromolecule: Name: Large ribosomal subunit protein bL25 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 22.466166 KDa

Sequence

String:

MAKSASNQLR VTVRTETGKG ASRRARRAGK IPAVLYGHGA EPQHLELPGH DYAAVLRHSG TNAVLTLDIA GKEQLALTKA LHIHPIRRT IQHADLLVVR RGEKVVVEVS VVVEGQAGPD TLVTQETNSI EIEAEALSIP EQLTVSIEGA EPGTQLTAGQ I ALPAGVSL ISDPDLLVVN VVKAPTAEEL EGEVAGAEEA EEAAVEAGEA EAAGESE

UniProtKB: Large ribosomal subunit protein bL25

Macromolecule #28: Large ribosomal subunit protein bL27

• Macromolecule: Name: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 8.987272 KDa

Sequence

String:

MAHKKGASSS RNGRDSAAQR LGVKRYGGQV VKAGEILVRQ RGTKFHPGVN VGRGGDDTLF AKTAGAVEFG IKRGRKTVSI VGSTTA

UniProtKB: Large ribosomal subunit protein bL27

Macromolecule #29: Large ribosomal subunit protein bL28

• Macromolecule: Name: Large ribosomal subunit protein bL28 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 6.920075 KDa

Sequence

String:

MAAVCDICGK GPGFGKSVSH SHRRTSRRWD PNIQTVHAVT RPGGNKKRLN VCTSCIKAGK ITRG

UniProtKB: UNIPROTKB: A0AAE8WTM0

Macromolecule #30: Large ribosomal subunit protein uL29

• Macromolecule: Name: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 8.872015 KDa

Sequence

String:

MAVGVSPGEL RELTDEELAE RLRESKEELF NLRFQMATGQ LNNNRRLRTV RQEIARIYTV LRERELGLAT GPDGKES

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #31: Large ribosomal subunit protein uL30

• Macromolecule: Name: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 7.361569 KDa

Sequence

String:

MSQLKITQVR STIGARWKQR ESLRTLGLRR IRHSVIREDN AATRGLIAVV RHLVEVEPAQ TGGKT

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #33: Small ribosomal subunit protein uS3

• Macromolecule: Name: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 30.071018 KDa

Sequence

String:

MGQKINPHGF RLGITTDWKS RWYADKQYAE YVKEDVAIRR LLSSGLERAG IADVEIERTR DRVRVDIHTA RPGIVIGRRG TEADRIRAD LEKLTGKQVQ LNILEVKNPE SQAQLVAQGV AEQLSNRVAF RRAMRKAIQS AMRQPNVKGI RVQCSGRLGG A EMSRSEFY REGRVPLHTL RADIDYGLYE AKTTFGRIGV KVWIYKGDIV GGKRELAAAA PAGADRPRRE RPSGTRPRRS GA SGTTATG TDAGRAAGGE EAAPDAAAPV EAQSTES

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #34: Small ribosomal subunit protein uS4

• Macromolecule: Name: Small ribosomal subunit protein uS4 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 23.515898 KDa

Sequence

String:

MARYTGPVTR KSRRLRTDLV GGDQAFEKRP YPPGQHGRAR IKESEYLLQL QEKQKARFTY GVMEKQFRRY YEEAVRQPGK TGEELLKIL ESRLDNVIYR AGLARTRRMA RQLVSHGHFN VNGVHVNVPS YRVSQYDIVD VRDKSLNTVP FQIARETAGE R PIPSWLQV VGERQRVLIH QLPERAQIDV PLTEQLIVEY YSK

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #35: Small ribosomal subunit protein uS5

• Macromolecule: Name: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 22.925242 KDa

Sequence

String:

MAEQPAGQAG TTDNRDARGD REGRRRDSGR GSRERDGEKS NYLERVVAIN RVSKVVKGGR RFSFTALVIV GDGNGMVGVG YGKAKEVPA AIAKGVEEAR KSFFRVPLIG GTITHPVQGE AAAGVVLLRP ASPGTGVIAG GAARAVLECA GVHDILAKSL G SDNAINVV HATVAALKLL QRPEEVAARR GLPIEDVAPA GMLKARRKSE ALAASVLPDR TI

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #36: Small ribosomal subunit protein bS6

• Macromolecule: Name: Small ribosomal subunit protein bS6 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 10.952728 KDa

Sequence

String:

MRPYEIMVIL DPTLDERTVA PSLETFLNVV RKDGGKVEKV DIWGKRRLAY EIAKHAEGIY VVIDVKAAPA TVSELDRQLS LNESVLRTK VMRTDKH

UniProtKB: Small ribosomal subunit protein bS6

Macromolecule #37: Small ribosomal subunit protein uS7

• Macromolecule: Name: Small ribosomal subunit protein uS7 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 17.633418 KDa

Sequence

String:

MPRKGPAPKR PLVNDPVYGS QLVTQLVNKV LLKGKKSLAE RIVYGALEQA RDKTGTDPVI TLKRALDNVK PALEVRSRRV GGATYQVPV EVRPDRSTTL ALRWLVGYSR QRREKTMIER LANEILDASN GLGASVKRRE DTHKMAEANR AFAHYRW

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #38: Small ribosomal subunit protein uS8

• Macromolecule: Name: Small ribosomal subunit protein uS8 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 14.432563 KDa

Sequence

String:

MTMTDPIADF LTRLRNANSA YHDEVSLPHS KLKANIAQIL KNEGYISDFR TEDARVGKSL VIQLKYGPSR ERSIAGLRRV SKPGLRVYA KSTNLPRVLG GLGVAIISTS SGLLTDRQAA RQGVGGEVLA YVW

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #39: Small ribosomal subunit protein uS9

• Macromolecule: Name: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 16.465061 KDa

Sequence

String:

MTETTPAPQT PAAPAGPAQS FVLERPIQTV GRRKEAVVRV RLVPGTGKFD LNGRSLEDYF PNKVHQQLIK APLVTVDRVE SFDIFAHLG GGGPSGQAGA LRLGIARALI LVSPEDRPAL KKAGFLTRDP RATERKKYGL KKARKAPQYS KR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #40: Small ribosomal subunit protein uS10

• Macromolecule: Name: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 11.45234 KDa

Sequence

String:

MAGQKIRIRL KAYDHEAIDA SARKIVETVV RTGASVVGPV PLPTEKNVYC VIRSPHKYKD SREHFEMRTH KRLIDIIDPT PKTVDALMR IDLPASVDVN IQ

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #41: Small ribosomal subunit protein uS11

• Macromolecule: Name: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 14.80084 KDa

Sequence

String:

MPPAKKGPAT SARKGQKTRR REKKNVPHGA AHIKSTFNNT IVTITDPQGN VIAWASSGHV GFKGSRKSTP FAAQLAAENA ARKAQDHGV RKVDVFVKGP GSGRETAIRS LQAAGLEVGA ISDVTPQPHN GVRPPNRRRV

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #42: Small ribosomal subunit protein uS12

• Macromolecule: Name: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 13.882341 KDa

Sequence

String:

MPTIQQLVRK GRRDKISKVK TAALKGSPQR RGVCTRVYTT TPKKPNSALR KVARVKLTSQ VEVTAYIPGE GHNLQEHSMV LVRGGRVKD LPGVRYKIIR GSLDTQGVKN RKQARSRYGA KKEKG

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #43: Small ribosomal subunit protein uS13

• Macromolecule: Name: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 14.383779 KDa

Sequence

String:

MARLVGVDLP RDKRMEVALT YIFGIGRTRS NEILAATGID RDLRTRDLTE EQLIHLRDYI EANLKVEGDL RREVQADIRR KIEIGCYQG LRHRRGMPVR GQRTKTNART RKGPKRTIAG KKKAR

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #44: Small ribosomal subunit protein uS14B

• Macromolecule: Name: Small ribosomal subunit protein uS14B / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 6.841249 KDa

Sequence

String:

MAKKALVNKA AGKPRFAVRA YTRCSKCGRP RAVYRKFGLC RICLREMAHA GELPGVQKSS W

UniProtKB: Small ribosomal subunit protein uS14B

Macromolecule #45: Small ribosomal subunit protein uS15

• Macromolecule: Name: Small ribosomal subunit protein uS15 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 10.500277 KDa

Sequence

String:

MALTAEQKKE ILRSYGLHET DTGSPEAQIA LLTKRIADLT EHLKVHKHDH HSRRGLLLLV GRRRRLIKYI SQIDVERYRS LIERLGLRR

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #46: Small ribosomal subunit protein bS16

• Macromolecule: Name: Small ribosomal subunit protein bS16 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 17.4661 KDa

Sequence

String:

MAVKIKLTRL GKIRNPQYRV AVADARTRRD GRAIEVIGRY HPKEEPSLIE INSERAQYWL SVGAQPTEPV LKLLKITGDW QKFKGLPGA QGRLKVAAPK PSKLEVFNAA LAAADGGPTT EATKPKKKSP AKKAAKAAEP APQPEQPDTP ALGGEQAELT A ES

UniProtKB: Small ribosomal subunit protein bS16

Macromolecule #47: Small ribosomal subunit protein uS17

• Macromolecule: Name: Small ribosomal subunit protein uS17 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 14.776373 KDa

Sequence

String:

MAEAKTGAKA APRVAKAAKA APKKAAPNDA EAIGAANAAN VKGPKHTPRT PKPRGRRKTR IGYVVSDKMQ KTIVVELEDR MRHPLYGKI IRTTKKVKAH DEDSVAGIGD RVSLMETRPL SATKRWRLVE ILEKAK

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #48: Small ribosomal subunit protein bS18A

• Macromolecule: Name: Small ribosomal subunit protein bS18A / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 9.565279 KDa

Sequence

String:

MAKSSKRRPA PEKPVKTRKC VFCAKKDQAI DYKDTALLRT YISERGKIRA RRVTGNCVQH QRDIALAVKN AREVALLPFT SSVR

UniProtKB: Small ribosomal subunit protein bS18A

Macromolecule #49: Small ribosomal subunit protein uS19

• Macromolecule: Name: Small ribosomal subunit protein uS19 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 10.830629 KDa

Sequence

String:

MPRSLKKGPF VDEHLLKKVD VQNEKNTKQV IKTWSRRSTI IPDFIGHTFA VHDGRKHVPV FVTESMVGHK LGEFAPTRTF KGHIKDDRK SKRR

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #50: Small ribosomal subunit protein bS20

• Macromolecule: Name: Small ribosomal subunit protein bS20 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 9.428941 KDa

Sequence

String:

MANIKSQQKR NRTNERARLR NKAVKSSLRT AVRAFREAAH AGDKAKAAEL LASTNRKLDK AASKGVIHKN QAANKKSALA QALNKL

UniProtKB: Small ribosomal subunit protein bS20

Macromolecule #51: Mitochondrial domain of uncharacterised function (DUF1713)

• Macromolecule: Name: Mitochondrial domain of uncharacterised function (DUF1713); type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria)
• Molecular weight: Theoretical: 8.310584 KDa

Sequence

String:

MGSVIKKRRK RMSKKKHRKL LRRTRVQRRK LGKMGSVIKK RRKRMSKKKH RKLLRRTRVQ RRKLGK

Macromolecule #7: 23S rRNA

• Macromolecule: Name: 23S rRNA / type: rna / ID: 7 / Number of copies: 1
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 1.017834562 MDa

Sequence

String:

UUGUAAGUGU CUAAGGGCGC AUGGUGGAUG CCUUGGCAUC GAGAGCCGAU GAAGGACGUG GGAGGCUGCG AUAUGCCUCG GGGAGCUGU CAACCGAGCG UGGAUCCGAG GAUUUCCGAA UGGGGAAACC CAGCACGAGU GAUGUCGUGC UACCCGCAUC U GAAUAUAU AGGGUGCGGG AGGGAACGCG GGGAAGUGAA ACAUCUCAGU ACCCGUAGGA GGAGAAAACA AUUGUGAUUC CG CAAGUAG UGGCGAGCGA ACGCGGAACA GGCUAAACCG CACGCAUGGG UAACCGGGUA GGGGUUGUGU GUGCGGGGUU GUG GGAGGA UAUGUCUCAG CGCUACCCGG CUGAGAGGCA GUCAGAAAGU GUCGUGGUUA GCGGAAGUGG CCUGGGAUGG UCUG CCGUA GACGGUGAGA GCCCGGUACG CGAAAACCCG GCACCUGCCU AGUAUCAAUU CCCGAGUAGC AGCGGGCCCG UGGAA UCCG CUGUGAAUCC GCCGGGACCA CCCGGUAAGC CUAAAUACUC CUCGAUGACC GAUAGCGGAU UAGUACCGUG AGGGAA UGG UGAAAAGUAC CCCGGGAGGG GAGUGAAAGA GUACCUGAAA CCGUGUGCCU ACAAUCCGUC AGAGCCUCCU UUUCCUC UC CGGAGGAGGG UGGUGAUGGC GUGCCUUUUG AAGAAUGAGC CUGCGAGUCA GGGACAUGUC GCAAGGUUAA CCCGUGUG G GGUAGCCGCA GCGAAAGCGA GUCUGAAUAG GGCGACCCAC ACGCGCAUAC GCGCGUGUGA AUAGUGGCGU GUUCUGGAC CCGAAGCGGA GUGAUCUACC CAUGGCCAGG GUGAAGCGCG GGUAAGACCG CGUGGAGGCC CGAACCCACU UAGGUUGAAG ACUGAGGGG AUGAGCUGUG GGUAGGGGUG AAAGGCCAAU CAAACUCCGU GAUAGCUGGU UCUCCCCGAA AUGCAUUUAG G UGCAGCGU UGCGUGGUUC ACCGCGGAGG UAGAGCUACU GGAUGGCCGA UGGGCCCUAC UAGGUUACUG ACGUCAGCCA AA CUCCGAA UGCCGUGGUG UAAAGCGUGG CAGUGAGACG GCGGGGGAUA AGCUCCGUAC GUCGAAAGGG AAACAGCCCA GAU CGCCGG CUAAGGCCCC CAAGCGUGUG CUAAGUGGGA AAGGAUGUGC AGUCGCAAAG ACAACCAGGA GGUUGGCUUA GAAG CAGCC ACCCUUGAAA GAGUGCGUAA UAGCUCACUG GUCAAGUGAU UGUGCGCCGA UAAUGUAGCG GGGCUCAAGC ACACC GCCG AAGCCGCGGC ACAUCCACCU UGUGGUGGGU GUGGGUAGGG GAGCGUCCCU CAUUCAGCGA AGCCACCGGG UGACCG GUG GUGGAGGGUG GGGGAGUGAG AAUGCAGGCA UGAGUAGCGA CAAGGCAAGU GAGAACCUUG CCCGCCGAAA GACCAAG GG UUCCUGGGCC AGGCCAGUCC GCCCAGGGUG AGUCGGGACC UAAGGCGAGG CCGACAGGCG UAGUCGAUGG ACAACGGG U UGAUAUUCCC GUACCCGUGU GUGGGCGCCC GUGACGAAUC AGCGGUACUA ACCACCCAAA ACCGGAUCGA UCACUCCCC UUCGGGGGUG UGGAGUUCUG GGGCUGCGUG GGAACUUCGC UGGUAGUAGU CAAGCGAAGG GGUGACGCAG GAAGGUAGCC GUACCAGUC AGUGGUAACA CUGGGGCAAG CCGGUAGGGA GAGCGAUAGG CAAAUCCGUC GCUCACUAAU CCUGAGAGGU G ACGCAUAG CCGGUUGAGG CGAAUUCGGU GAUCCUCUGC UGCCAAGAAA AGCCUCUAGC GAGCACACAC ACGGCCCGUA CC CCAAACC GACACAGGUG GUCAGGUAGA GCAUACCAAG GCGUACGAGA UAACUAUGGU UAAGGAACUC GGCAAAAUGC CCC CGUAAC UUCGGGAGAA GGGGGACCGG AAUAUCGUGA ACACCCUUGC GGUGGGAGCG GGAUCCGGUC GCAGAAACCA GUGA GGAGC GACUGUUUAC UAAAAACACA GGUCCGUGCG AAGUCGCAAG ACGAUGUAUA CGGACUGACG CCUGCCCGGU GCUGG AAGG UUAAGAGGAC CCGUUAACCC GCAAGGGUGA AGCGGAGAAU UUAAGCCCCA GUAAACGGCG GUGGUAACUA UAACCA UCC UAAGGUAGCG AAA(5MU)UCCUUG UCGGGUAAGU UCCGACCUGC ACGAAUGGCG UAACGACUUC UCAACUGUCU CAA CCAUAG ACUCGGCGAA AUUGCACUAC GAGUAAAGAU GCUCGUUACG CGCGGCAGGA CGAAAAGACC CCGGGACCUU CACU ACAAC UUGGUAUUGA UGUUCGGUAC GGUUUGUGUA GGAUAGGUGG GAGACUGUGA AACCUCGACG CCAGUUGGGG CGGAG UCGU UGUUGAAAUA CCACUCUGAU CGUAUUGGGC AUCUAACCUC GAACCCUGAA UCGGGUUUAG GGACAGUGCC UGGCGG GUA GUUUAACUG(OMG) GGCGGUUGCC UCCUAAAAUG UAACGGAGGC GCCCAAAGGU UCCCUCAACC UGGACGGCAA UCA GGUGGC GAGUGUAAAU GCACAAGGGA GCUUGACUGC GAGACUUACA AGUCAAGCAG GGACGAAAGU CGGGAUUAGU GAUC CGGCA CCCCCGAGUG GAAGGGGUGU CGCUCAACGG AUAAAAGGUA CCCCGGGGAU AACAGGCUGA UCUUCCCCAA GAGUC CAUA UCGACGGGAU GGUUUGGCAC CUCGAUGUCG GCUCGUCGCA UCCUGGGGCU GGAGCAGGUC CCAAGGGUUG GGCU (OMG)UUCG CCCAUUAAAG CGGCACGCGA GCUGGGUUUA GAACGUCGUG AGACAGUUCG GUCUCUAUCC GCCGCGCGCG U CAGAAACU UGAGGAAACC UGUCCCUAGU ACGAGAGGAC CGGGACGGAC GAACCUCUGG UGCACCAGUU GUCCCGCCAG GG GCACCGC UGGAUAGCCA CGUUCGGUCA GGAUAACCGC UGAAAGCAUC UAAGCGGGAA ACCUUCUCCA AGAUCAGGUU UCU CACCCA CUUGGUGGGA UAAGGCCCCC CGCAGAACAC GGGUUCAAUA GGUCAGACCU GGAAGCUCAG UAAUGGGUGU AGGG AACUG GUGCUAACCG GCCGAAAACU UACAACA

GENBANK: GENBANK: CP033310.1

Macromolecule #8: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 8 / Number of copies: 1
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 37.097238 KDa

Sequence

String:

UUACGGCGGC CACAGCGGCA GGGAAACGCC CGGUCCCAUU CCGAACCCGG AAGCUAAGCC UGCCAGCGCC GAUGAUACUG CCCCUCCGG GUGGAAAAGU AGGACACCGC CGAACA

GENBANK: GENBANK: CP033310.1

Macromolecule #32: 16S rRNA

• Macromolecule: Name: 16S rRNA / type: rna / ID: 32 / Number of copies: 1
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria); Strain: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2
• Molecular weight: Theoretical: 498.40175 KDa

Sequence

String:

UUUUGUUUGG AGAGUUUGAU CCUGGCUCAG GACGAACGCU GGCGGCGUGC UUAACACAUG CAAGUCGAAC GGAAAGGUCU CUUCGGAGA UACUCGAGUG GCGAACGGGU GAGUAACACG UGGGUGAUCU GCCCUGCACU UCGGGAUAAG CCUGGGAAAC U GGGUCUAA UACCGGAUAG GACCACGGGA UGCAUGUCUU GUGGUGGAAA GCGCUUUAGC GGUGUGGGAU GAGCCCGCGG CC UAUCAGC UUGUUGGUGG GGUGACGGCC UACCAAGGCG ACGACGGGUA GCCGGCCUGA GAGGGUGUCC GGCCACACUG GGA CUGAGA UACGGCCCAG ACUCCUACGG GAGGCAGCAG UGGGGAAUAU UGCACAAUGG GCGCAAGCCU GAUGCAGCGA CGCC GCGUG GGGGAUGACG GCCUUCGGGU UGUAAACCUC UUUCACCAUC GACGAAGGUC CGGGUUCUCU CGGAUUGACG GUAGG UGGA GAAGAAGCAC CGGCCAACUA CGUGCCAGCA GCC(G7M)CGGUAA UACGUAGGGU GCGAGCGUUG UCCGGAAUUA CU GGGCGUA AAGAGCUCGU AGGUGGUUUG UCGCGUUGUU CGUGAAAUCU CACGGCUUAA CUGUGAGCGU GCGGGCGAUA CGG GCAGAC UAGAGUACUG CAGGGGAGAC UGGAAUUCCU GGUGUAGCGG UGGAAUGCGC AGAUAUCAGG AGGAACACCG GUGG CGAAG GCGGGUCUCU GGGCAGUAAC UGACGCUGAG GAGCGAAAGC GUGGGGAGCG AACAGGAUUA GAUACCCUGG UAGUC CACG CCGUAAACGG UGGGUACUAG GUGUGGGUUU CCUUCCUUGG GAUCCGUGCC GUAGCUAACG CAUUAAGUAC CCCGCC UGG GGAGUACGGC CGCAAGGCUA AAACUCAAAG GAAUUGACGG GGGCCCGCAC AAGCGGCGGA GCAUGUGGAU UAAUUCG AU (2MG)(5MC)AACGCGAA GAACCUUACC UGGGUUUGAC AUGCACAGGA CGCGUCUAGA GAUAGGCGUU CCCUUGUGGC CUGUGUGCA GGUGGUGCAU GGCUGUCGUC AGCUCGUGUC GUGAGAUGUU GGGUUAAGUC CCGCAACGAG CGCAACCCUU G UCUCAUGU UGCCAGCACG UAAUGGUGGG GACUCGUGAG AGACUGCCGG GGUCAACUCG GAGGAAGGUG GGGAUGACGU CA AGUCAUC AUGCCCCUUA UGUCCAGGGC UUCACACAUG CUACAAUGGC CGGUACAAAG GGCUGCGAUG CCGCGAGGUU AAG CGAAUC CUUAAAAGCC GGUCUCAGUU CGGAUCGGGG UCUGCAACUC GACCCCGUGA AGUCGGAGUC GCUAGUAAUC GCAG AUCAG CAACGCUGCG GUGAAUACGU UCCCGGGCCU UGUACACACC G(4OC)CCGUCACG UCAUGAAAGU CGGUAACACC C GAAGCCAG UGGCCUAACC CUCGGGAGGG AGCUGUCGAA GGUGGGAUCG GCGAUUGGGA CGAAGUCG(UR3)A ACAAGGUA G CCGUACCGG(MA6) (MA6)GGUGCGGCU GGAUCACCUC CUUUCU

GENBANK: GENBANK: CP033311.1

Macromolecule #52: tRNA

• Macromolecule: Name: tRNA / type: rna / ID: 52 / Number of copies: 1
• Source (natural): Organism: Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 (bacteria)
• Molecular weight: Theoretical: 894.612 Da

Sequence

String:

CCA

Macromolecule #53: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 53 / Number of copies: 5 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #54: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 54 / Number of copies: 441 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #55: (2~{R},3~{R},4~{R},5~{R},8~{R},10~{R},11~{R},12~{S},13~{S},14~{R}...

• Macromolecule: Name: (2~{R},3~{R},4~{R},5~{R},8~{R},10~{R},11~{R},12~{S},13~{S},14~{R})-11-[(2~{S},3~{R},4~{S},6~{R})-4-(dimethylamino)-6-methyl-3-oxidanyl-oxan-2-yl]oxy-2-ethyl-4-[(2~{R},3~{R},4~{R},5~{S},6~{R})-6-(hydroxymethyl)-3,4-bis(oxidanyl)-5-[[4-(4-pyridin-4-yl-1,2,3-triazol-1-yl)phenyl]methoxy]oxan-2-yl]oxy-13-[(2~{R},4~{R},5~{S},6~{S})-4-methoxy-4,6-dimethyl-5-oxidanyl-oxan-2-yl]oxy-3,5,6,8,10,12,14-heptamethyl-3,10-bis(oxidanyl)-1-oxa-6-azacyclopentadecan-15-one; type: ligand / ID: 55 / Number of copies: 1 / Formula: A1L32
• Molecular weight: Theoretical: 1.145381 KDa

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.6
• Sugar embedding: Material: vitrified ice
• Grid: Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 10 sec.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: JEOL CRYO ARM 300
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 40.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 60000
• Sample stage: Specimen holder model: JEOL CRYOSPECPORTER / Cooling holder cryogen: NITROGEN

Image processing

• Particle selection: Number selected: 515051
• Startup model: Type of model: NONE
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.33 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 4.0.1) / Number images used: 205461
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 4.0.1)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 4.0.1)
• Final 3D classification: Software - Name: RELION (ver. 4.0.1)