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- EMDB-60983: Iterative acetyltransferase on lasso peptides from Actinomycetes ... -
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Open data
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Basic information
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Title | Iterative acetyltransferase on lasso peptides from Actinomycetes in complex with CoA | ||||||||||||||||||||||||
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![]() | GCN5-related N-acetyltransferases / Actinomycetes / CoA / TRANSFERASE | ||||||||||||||||||||||||
Function / homology | Acetyltransferase (GNAT) domain / acyltransferase activity, transferring groups other than amino-acyl groups / Gcn5-related N-acetyltransferase (GNAT) domain profile. / GNAT domain / Acyl-CoA N-acyltransferase / GCN5-related N-acetyltransferase![]() | ||||||||||||||||||||||||
Biological species | ![]() ![]() ![]() | ||||||||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.17 Å | ||||||||||||||||||||||||
![]() | Wu S / Xiong J / Lei D / Dong S | ||||||||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Iterative acylation on mature lasso peptides by widespread GNATs for lipolasso production Authors: Xiong J / Wu S / Liang Z / Gong X / Fang S / Tao F / Wu Q / Cui J / Gao K / Luo S / Lei D / Dong S | ||||||||||||||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 29 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 18.7 KB 18.7 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 9.5 KB | Display | ![]() |
Images | ![]() | 148.8 KB | ||
Masks | ![]() | 64 MB | ![]() | |
Filedesc metadata | ![]() | 5.5 KB | ||
Others | ![]() ![]() | 59.1 MB 59.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 810.9 KB | Display | ![]() |
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Full document | ![]() | 810.4 KB | Display | |
Data in XML | ![]() | 16.4 KB | Display | |
Data in CIF | ![]() | 21.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 9iy3MC ![]() 9iy4C M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.831 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Density Histograms |
-Half map: #2
File | emd_60983_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_60983_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Complex of iterative acetyltransferase and CoA
Entire | Name: Complex of iterative acetyltransferase and CoA |
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Components |
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-Supramolecule #1: Complex of iterative acetyltransferase and CoA
Supramolecule | Name: Complex of iterative acetyltransferase and CoA / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Supramolecule #2: Iterative acetyltransferase
Supramolecule | Name: Iterative acetyltransferase / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: GCN5-related N-acetyltransferase
Macromolecule | Name: GCN5-related N-acetyltransferase / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 21.676164 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MSPQDDLIVW LRGDRAGLGP FSADLVDQYW RWEQDPSVLI GYGRQTPDSL ENRREGYGHQ ARGTDDQLRF TVYDLTGQDP VPVGTTAVL IDHHVRTGEF VIQLGAGHRG RGLGTEATRL TLDYAFHVSA LACVHLAVLT PNTGAIAAYE RAGFRRIGER R DSGFWLGR ...String: MSPQDDLIVW LRGDRAGLGP FSADLVDQYW RWEQDPSVLI GYGRQTPDSL ENRREGYGHQ ARGTDDQLRF TVYDLTGQDP VPVGTTAVL IDHHVRTGEF VIQLGAGHRG RGLGTEATRL TLDYAFHVSA LACVHLAVLT PNTGAIAAYE RAGFRRIGER R DSGFWLGR RVSETLMDAV PEDFPGPSVV RGFVEGGR UniProtKB: GCN5-related N-acetyltransferase |
-Macromolecule #2: COENZYME A
Macromolecule | Name: COENZYME A / type: ligand / ID: 2 / Number of copies: 1 / Formula: COA |
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Molecular weight | Theoretical: 767.534 Da |
Chemical component information | ![]() ChemComp-COA: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |