+ Open data
Open data
- Basic information
Basic information
| Entry |  | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Title | The local map of the TEF30-associated PSII core in TEF30-C2S | |||||||||
|  Map data | ||||||||||
|  Sample | 
 | |||||||||
|  Keywords | photosystem II / PSII repair / PSII assembly / PSII intermediate / TEF30 / C2S / supercomplex / PHOTOSYNTHESIS | |||||||||
| Biological species |   Chlamydomonas reinhardtii (plant) | |||||||||
| Method | single particle reconstruction / cryo EM / Resolution: 2.9 Å | |||||||||
|  Authors | Wang Y / Liu Z | |||||||||
| Funding support |  China, 1 items 
 | |||||||||
|  Citation |  Journal: To Be Published Title: TEF30 fulfills versatile functions in the photosystem II repair process Authors: Wang Y / Wang C / Li A / Liu Z | |||||||||
| History | 
 | 
- Structure visualization
Structure visualization
| Supplemental images | 
|---|
- Downloads & links
Downloads & links
-EMDB archive
| Map data |  emd_60828.map.gz | 121.3 MB |  EMDB map data format | |
|---|---|---|---|---|
| Header (meta data) |  emd-60828-v30.xml  emd-60828.xml | 16.8 KB 16.8 KB | Display Display |  EMDB header | 
| FSC (resolution estimation) |  emd_60828_fsc.xml | 14.9 KB | Display |  FSC data file | 
| Images |  emd_60828.png | 83.9 KB | ||
| Filedesc metadata |  emd-60828.cif.gz | 3.8 KB | ||
| Others |  emd_60828_half_map_1.map.gz  emd_60828_half_map_2.map.gz | 226.8 MB 226.8 MB | ||
| Archive directory |  http://ftp.pdbj.org/pub/emdb/structures/EMD-60828  ftp://ftp.pdbj.org/pub/emdb/structures/EMD-60828 | HTTPS FTP | 
-Validation report
| Summary document |  emd_60828_validation.pdf.gz | 1.2 MB | Display |  EMDB validaton report | 
|---|---|---|---|---|
| Full document |  emd_60828_full_validation.pdf.gz | 1.2 MB | Display | |
| Data in XML |  emd_60828_validation.xml.gz | 22.4 KB | Display | |
| Data in CIF |  emd_60828_validation.cif.gz | 29.1 KB | Display | |
| Arichive directory |  https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-60828  ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-60828 | HTTPS FTP | 
-Related structure data
- Links
Links
| EMDB pages |  EMDB (EBI/PDBe) /  EMDataResource | 
|---|
- Map
Map
| File |  Download / File: emd_60828.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Projections & slices | Image control
 
 Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
| Voxel size | X=Y=Z: 1.06 Å | ||||||||||||||||||||||||||||||||||||
| Density | 
 | ||||||||||||||||||||||||||||||||||||
| Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
| Details | EMDB XML: 
 | 
-Supplemental data
-Half map: #1
| File | emd_60828_half_map_1.map | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Projections & Slices | 
 | ||||||||||||
| Density Histograms | 
-Half map: #2
| File | emd_60828_half_map_2.map | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Projections & Slices | 
 | ||||||||||||
| Density Histograms | 
- Sample components
Sample components
-Entire : TEF30-associated C2S-type PSII-LHCII supercomplex
| Entire | Name: TEF30-associated C2S-type PSII-LHCII supercomplex | 
|---|---|
| Components | 
 | 
-Supramolecule #1: TEF30-associated C2S-type PSII-LHCII supercomplex
| Supramolecule | Name: TEF30-associated C2S-type PSII-LHCII supercomplex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#33 | 
|---|---|
| Source (natural) | Organism:   Chlamydomonas reinhardtii (plant) | 
-Experimental details
-Structure determination
| Method | cryo EM | 
|---|---|
|  Processing | single particle reconstruction | 
| Aggregation state | particle | 
- Sample preparation
Sample preparation
| Buffer | pH: 7.2 | 
|---|---|
| Vitrification | Cryogen name: ETHANE | 
- Electron microscopy
Electron microscopy
| Microscope | FEI TITAN KRIOS | 
|---|---|
| Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 | 
| Electron beam | Acceleration voltage: 300 kV / Electron source:  FIELD EMISSION GUN | 
| Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.3 µm | 
| Experimental equipment |  Model: Titan Krios / Image courtesy: FEI Company | 
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