[English] 日本語
Yorodumi- EMDB-60826: Cryo-EM structure of human ASCT2 (SLC1A5) at 2.53 angstrom resolu... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-60826 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Cryo-EM structure of human ASCT2 (SLC1A5) at 2.53 angstrom resolution from Biortus | |||||||||
Map data | ||||||||||
Sample |
| |||||||||
Keywords | Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A5 subfamily. / MEMBRANE PROTEIN | |||||||||
Biological species | Homo sapiens (Human) (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.53 Å | |||||||||
Authors | Shi H / Yang Y / Han T / Hu Y | |||||||||
Funding support | 1 items
| |||||||||
Citation | Journal: To Be Published Title: Cryo-EM structure of human ASCT2 at 2.53 angstrom resolution from Biortus Authors: Shi H / Yang Y / Han T / Hu Y | |||||||||
History |
|
-Structure visualization
Supplemental images |
---|
-Downloads & links
-EMDB archive
Map data | emd_60826.map.gz | 122.8 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-60826-v30.xml emd-60826.xml | 11.1 KB 11.1 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_60826_fsc.xml | 13.1 KB | Display | FSC data file |
Images | emd_60826.png | 99.6 KB | ||
Masks | emd_60826_msk_1.map | 244.1 MB | Mask map | |
Filedesc metadata | emd-60826.cif.gz | 3.7 KB | ||
Others | emd_60826_half_map_1.map.gz emd_60826_half_map_2.map.gz | 226.8 MB 226.8 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-60826 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-60826 | HTTPS FTP |
-Validation report
Summary document | emd_60826_validation.pdf.gz | 860.6 KB | Display | EMDB validaton report |
---|---|---|---|---|
Full document | emd_60826_full_validation.pdf.gz | 860.1 KB | Display | |
Data in XML | emd_60826_validation.xml.gz | 21.6 KB | Display | |
Data in CIF | emd_60826_validation.cif.gz | 27.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-60826 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-60826 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|
-Map
File | Download / File: emd_60826.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.925 Å | ||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Mask #1
File | emd_60826_msk_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #1
File | emd_60826_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #2
File | emd_60826_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Sample components
-Entire : Neutral amino acid transporter B(0)
Entire | Name: Neutral amino acid transporter B(0) |
---|---|
Components |
|
-Supramolecule #1: Neutral amino acid transporter B(0)
Supramolecule | Name: Neutral amino acid transporter B(0) / type: organelle_or_cellular_component / ID: 1 / Parent: 0 |
---|---|
Source (natural) | Organism: Homo sapiens (Human) (human) |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
---|---|
Vitrification | Cryogen name: NITROGEN |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |