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Yorodumi- EMDB-60820: Cryo-EM structure of human CCR8 in the inactive form at 3.15 angs... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-60820 | |||||||||
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Title | Cryo-EM structure of human CCR8 in the inactive form at 3.15 angstrom resolution from Biortus | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Belongs to the G-protein coupled receptor 1 family (Protein from Homo sapiens) / MEMBRANE PROTEIN | |||||||||
Biological species | Homo sapiens (Human) (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.15 Å | |||||||||
Authors | Shi H / Yang Y / Hu J / Hu Y | |||||||||
Funding support | 1 items
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Citation | Journal: To Be Published Title: Cryo-EM structure of human CCR8 in the inactive form at 3.15 angstrom resolution from Biortus Authors: Shi H / Yang Y / Hu J / Hu Y | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_60820.map.gz | 122.1 MB | EMDB map data format | |
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Header (meta data) | emd-60820-v30.xml emd-60820.xml | 11.1 KB 11.1 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_60820_fsc.xml | 13.2 KB | Display | FSC data file |
Images | emd_60820.png | 77.3 KB | ||
Masks | emd_60820_msk_1.map | 244.1 MB | Mask map | |
Filedesc metadata | emd-60820.cif.gz | 3.7 KB | ||
Others | emd_60820_half_map_1.map.gz emd_60820_half_map_2.map.gz | 226.5 MB 226.5 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-60820 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-60820 | HTTPS FTP |
-Validation report
Summary document | emd_60820_validation.pdf.gz | 861.1 KB | Display | EMDB validaton report |
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Full document | emd_60820_full_validation.pdf.gz | 860.6 KB | Display | |
Data in XML | emd_60820_validation.xml.gz | 21.4 KB | Display | |
Data in CIF | emd_60820_validation.cif.gz | 27.5 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-60820 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-60820 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_60820.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.925 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_60820_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_60820_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_60820_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : C-C chemokine receptor type 8
Entire | Name: C-C chemokine receptor type 8 |
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Components |
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-Supramolecule #1: C-C chemokine receptor type 8
Supramolecule | Name: C-C chemokine receptor type 8 / type: organelle_or_cellular_component / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Homo sapiens (Human) (human) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: NITROGEN |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |