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- EMDB-60662: human SLC26A7 apo state -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-60662
Titlehuman SLC26A7 apo state
Map data
Sample
  • Complex: Structure of human SLC26A7 in apo state
    • Protein or peptide: Anion exchange transporter
Keywordstransport protein
Function / homology
Function and homology information


gluconate transmembrane transport / oxalate transport / nitrate transmembrane transport / Multifunctional anion exchangers / gastric acid secretion / iodide transport / sulfate transmembrane transporter activity / oxalate transmembrane transporter activity / sulfate transmembrane transport / chloride:bicarbonate antiporter activity ...gluconate transmembrane transport / oxalate transport / nitrate transmembrane transport / Multifunctional anion exchangers / gastric acid secretion / iodide transport / sulfate transmembrane transporter activity / oxalate transmembrane transporter activity / sulfate transmembrane transport / chloride:bicarbonate antiporter activity / thyroid hormone generation / bicarbonate transport / bicarbonate transmembrane transporter activity / chloride transmembrane transporter activity / chloride transport / chloride channel activity / lateral plasma membrane / monoatomic ion transport / chloride transmembrane transport / recycling endosome membrane / basolateral plasma membrane / endosome / apical plasma membrane / plasma membrane / cytoplasm
Similarity search - Function
SLC26A/SulP transporter / SLC26A/SulP transporter domain / Sulfate permease family / STAS domain / STAS domain profile. / STAS domain / STAS domain superfamily
Similarity search - Domain/homology
Anion exchange transporter
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsLi X / Yang X / Lu X / Lin B / Zhang Y / Huang B / Zhou Y / Huang J / Wu K / Zhou Q / Chi X
Funding support China, 1 items
OrganizationGrant numberCountry
National Science Foundation (NSF, China)32100975 China
CitationJournal: To Be Published
Title: human SLC26A7 apo state
Authors: Li X / Yang X / Lu X / Lin B / Zhang Y / Huang B / Zhou Y / Huang J / Wu K / Zhou Q / Chi X
History
DepositionJun 29, 2024-
Header (metadata) releaseJul 2, 2025-
Map releaseJul 2, 2025-
UpdateJul 2, 2025-
Current statusJul 2, 2025Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_60662.png

Downloads & links

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Map

FileDownload / File: emd_60662.map.gz / Format: CCP4 / Size: 38.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.09 Å/pix.
x 216 pix.
= 234.792 Å		1.09 Å/pix.
x 216 pix.
= 234.792 Å		1.09 Å/pix.
x 216 pix.
= 234.792 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.087 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.03
Minimum - Maximum-0.058445357 - 0.11339872
Average (Standard dev.)0.00041354544 (±0.004195105)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions216216216
Spacing216216216
CellA=B=C: 234.792 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_60662_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_60662_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Structure of human SLC26A7 in apo state

EntireName: Structure of human SLC26A7 in apo state
Components
  • Complex: Structure of human SLC26A7 in apo state
    • Protein or peptide: Anion exchange transporter

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Supramolecule #1: Structure of human SLC26A7 in apo state

SupramoleculeName: Structure of human SLC26A7 in apo state / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Anion exchange transporter

MacromoleculeName: Anion exchange transporter / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 72.275523 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MTGAKRKKKS MLWSKMHTPQ CEDIIQWCRR RLPILDWAPH YNLKENLLPD TVSGIMLAVQ QVTQGLAFAV LSSVHPVFGL YGSLFPAII YAIFGMGHHV ATGTFALTSL ISANAVERIV PQNMQNLTTQ SNTSVLGLSD FEMQRIHVAA AVSFLGGVIQ V AMFVLQLG ...String:
MTGAKRKKKS MLWSKMHTPQ CEDIIQWCRR RLPILDWAPH YNLKENLLPD TVSGIMLAVQ QVTQGLAFAV LSSVHPVFGL YGSLFPAII YAIFGMGHHV ATGTFALTSL ISANAVERIV PQNMQNLTTQ SNTSVLGLSD FEMQRIHVAA AVSFLGGVIQ V AMFVLQLG SATFVVTEPV ISAMTTGAAT HVVTSQVKYL LGMKMPYISG PLGFFYIYAY VFENIKSVRL EALLLSLLSI VV LVLVKEL NEQFKRKIKV VLPVDLVLII AASFACYCTN MENTYGLEVV GHIPQGIPSP RAPPMNILSA VITEAFGVAL VGY VASLAL AQGSAKKFKY SIDDNQEFLA HGLSNIVSSF FFCIPSAAAM GRTAGLYSTG AKTQVACLIS CIFVLIVIYA IGPL LYWLP MCVLASIIVV GLKGMLIQFR DLKKYWNVDK IDWGIWVSTY VFTICFAANV GLLFGVVCTI AIVIGRFPRA MTVSI KNMK EMEFKVKTEM DSETLQQVKI ISINNPLVFL NAKKFYTDLM NMIQKENACN QPLDDISKCE QNTLLNSLSN GNCNEE ASQ SCPNEKCYLI LDCSGFTFFD YSGVSMLVEV YMDCKGRSVD VLLAHCTASL IKAMTYYGNL DSEKPIFFES VSAAISH IH SNKNLSKLSD HSEV

UniProtKB: Anion exchange transporter

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 208237
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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