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- EMDB-60478: CryoEM structure of a cellulose CelS in monomeric form -

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Basic information

Entry
Database: EMDB / ID: EMD-60478
TitleCryoEM structure of a cellulose CelS in monomeric form
Map data
Sample
  • Complex: Monomeric form a cellulose CelS mutant
    • Protein or peptide: cellulose CelS
KeywordsCellulose mutant / HYDROLASE
Biological speciesAcetivibrio thermocellus (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.84 Å
AuthorsJiang WX / Cheng XQ / Ma LX / Cao Z / Xing Q
Funding support China, 2 items
OrganizationGrant numberCountry
National Basic Research Program of China (973 Program)2021YFC2100100 China
National Science Foundation (NSF, China)32371277 China
CitationJournal: To Be Published
Title: CryoEM structure of a cellulose CelS in monomeric form
Authors: Jiang WX / Cheng XQ / Ma LX / Cao Z / Xing Q
History
DepositionJun 7, 2024-
Header (metadata) releaseJun 11, 2025-
Map releaseJun 11, 2025-
UpdateJun 11, 2025-
Current statusJun 11, 2025Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_60478.png

Downloads & links

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Map

FileDownload / File: emd_60478.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.56 Å/pix.
x 320 pix.
= 180.224 Å		0.56 Å/pix.
x 320 pix.
= 180.224 Å		0.56 Å/pix.
x 320 pix.
= 180.224 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.5632 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.073
Minimum - Maximum-0.60879934 - 0.85110116
Average (Standard dev.)0.0000729329 (±0.022943638)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 180.224 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_60478_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_60478_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Monomeric form a cellulose CelS mutant

EntireName: Monomeric form a cellulose CelS mutant
Components
  • Complex: Monomeric form a cellulose CelS mutant
    • Protein or peptide: cellulose CelS

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Supramolecule #1: Monomeric form a cellulose CelS mutant

SupramoleculeName: Monomeric form a cellulose CelS mutant / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Acetivibrio thermocellus (bacteria)

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Macromolecule #1: cellulose CelS

MacromoleculeName: cellulose CelS / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Acetivibrio thermocellus (bacteria)
Molecular weightTheoretical: 70.796164 KDa
Recombinant expressionOrganism: Escherichia coli BL21 (bacteria)
SequenceString: MYKQRFLELW EELHDPSNGY FSSHGIPYHA VETLIVEAPD YGHLTTSEAM SYYLWLEALY GKFTGDFSYF MKAWETIEKY MIPDSTEQP GMSSYNPNSP ATYADEYEDP SYYPSELKFD TVRVGSDPVH NDLVSAYGPN MYLMHWLMDV DNWYGFGTGT R ATFINTFQ ...String:
MYKQRFLELW EELHDPSNGY FSSHGIPYHA VETLIVEAPD YGHLTTSEAM SYYLWLEALY GKFTGDFSYF MKAWETIEKY MIPDSTEQP GMSSYNPNSP ATYADEYEDP SYYPSELKFD TVRVGSDPVH NDLVSAYGPN MYLMHWLMDV DNWYGFGTGT R ATFINTFQ RGEQESTWET IPHPSIEEFK YGGPNGFLDL FTKDRSYAKQ WRYTNAPDAE GRAIQATYWA NQWAKEHGVN LS QYVKKAS RMGDFLRNDM FDKYFMKIGA QDKTPATGYD SAHYLMAWYT AWGGGIGASW AWKIGCSHAH AGYQNPMTAW ILA NDPEFK PESPNGANDW AKSLERQLEF YQWLQSAEGG IAGGATNSWN GRYEKYPAGT STFYGMAYVP HPVYADPGSN QWFG FQAWS MQRVMEYYLE TGDSSVKNLI KKWVDWVMSE IKLYDDGTFA IPSDLEWSGQ PDTWTGTYTG NPNLHVRVTS YGTDL GVAG SLANALATYA AATERWEGKL DTKARDMAAE LLDRMWNLYR DDKGLSAPET REDYVRFFEQ EVYVPQGWSG TMPNGD RIE PGVTFLDIRS KYLNDPDYPK LQQAYNEGKA PVFNYHRFWA QCDIAIANGL YSILF

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.2
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 38.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: DIFFRACTION / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.84 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 112442
Initial angle assignmentType: OTHER
Final angle assignmentType: NOT APPLICABLE
FSC plot (resolution estimation)

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