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- EMDB-60391: Cryo-EM structure of the N2-4N2C402 complex at a resolution of 1.... -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Cryo-EM structure of the N2-4N2C402 complex at a resolution of 1.9 angstrom | |||||||||
![]() | Cryo-EM map of the N2-4N2C402 complex | |||||||||
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![]() | viral enzyme / antibody / complex / inhibitor / VIRAL PROTEIN/IMMUNE SYSTEM / VIRAL PROTEIN-IMMUNE SYSTEM complex | |||||||||
Function / homology | ![]() exo-alpha-sialidase / exo-alpha-sialidase activity / viral budding from plasma membrane / carbohydrate metabolic process / host cell plasma membrane / virion membrane / metal ion binding / membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 1.9 Å | |||||||||
![]() | Chu BX / Wang X / Yang Q / Wu XD / Zhang ZL | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Profiling influenza neuraminidase pre-existing humoral immunity in humans Authors: Wang X / Yang Q / Chu BX / Wu XD / Zhang ZL | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 154.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 18.9 KB 18.9 KB | Display Display | ![]() |
Images | ![]() | 152.1 KB | ||
Filedesc metadata | ![]() | 6.4 KB | ||
Others | ![]() ![]() | 151.5 MB 151.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 805.5 KB | Display | ![]() |
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Full document | ![]() | 805.1 KB | Display | |
Data in XML | ![]() | 14 KB | Display | |
Data in CIF | ![]() | 16.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8zr4MC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||
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Annotation | Cryo-EM map of the N2-4N2C402 complex | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.92 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: halfmap A
File | emd_60391_half_map_1.map | ||||||||||||
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Annotation | halfmap_A | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: halfmap B
File | emd_60391_half_map_2.map | ||||||||||||
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Annotation | halfmap_B | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : A complex of N2 with 4N2C402-Fab
Entire | Name: A complex of N2 with 4N2C402-Fab |
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Components |
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-Supramolecule #1: A complex of N2 with 4N2C402-Fab
Supramolecule | Name: A complex of N2 with 4N2C402-Fab / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 500 KDa |
-Macromolecule #1: 4N2C402_Fab_H
Macromolecule | Name: 4N2C402_Fab_H / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 15.059547 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: QGRLQESGGG VVQPRRSLTL SCAASGFTLE TYTMHWVRQT PGKGLEWVAV SSKDGNNVYY RDSVKGRFTV SRDNSKNTLF LQMNHLRAE DTALYYCARG SDPDYDKGWG AYRNTDRPSY DGLDVWGQGT TVTVSS |
-Macromolecule #2: 4N2C402_Fab_L
Macromolecule | Name: 4N2C402_Fab_L / type: protein_or_peptide / ID: 2 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 12.490856 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: DIVMTQSPLF LSVTPGESAS ISCRSSQSLL HSNGYNYLDW YLQKPGQSPQ LLIYWGSNRA SGVSDRFSGR GSGTDFTLTI YNVEAEDVG VYYCMQALQT PPWTFGQGTK VDIK |
-Macromolecule #3: Neuraminidase
Macromolecule | Name: Neuraminidase / type: protein_or_peptide / ID: 3 / Number of copies: 4 / Enantiomer: LEVO / EC number: exo-alpha-sialidase |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 43.361598 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: ICPKPAEYRN WSKPQCGITG FAPFSKDNSI RLSAGGDIWV TREPYVSCDP DKCYQFALGQ GTTINNVHSN NTARDRTPHR TLLMNELGV PFHLGTKQVC IAWSSSSCHD GKAWLHVCIT GDDKNATASF IYNGRLVDSV VSWSKDILRT QESECVCING T CTVVMTDG ...String: ICPKPAEYRN WSKPQCGITG FAPFSKDNSI RLSAGGDIWV TREPYVSCDP DKCYQFALGQ GTTINNVHSN NTARDRTPHR TLLMNELGV PFHLGTKQVC IAWSSSSCHD GKAWLHVCIT GDDKNATASF IYNGRLVDSV VSWSKDILRT QESECVCING T CTVVMTDG NATGKADTKI LFIEEGKIVH TSKLSGSAQH VEECSCYPRY PGVRCVCRDN WKGSNRPIVD INIKDHSIVS SY VCSGLVG DTPRKTDSSS SSHCLNPNNE KGGHGVKGWA FDDGNDVWMG RTINETSRLG YETFKVVEGW SNPKSKLQIN RQV IVDRGD RSGYSGIFSV EGKSCINRCF YVELIRGRKE ETEVLWTSNS IVVFCGTSGT YGTGSWPDGA DLNLMHI UniProtKB: Neuraminidase |
-Macromolecule #5: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 5 / Number of copies: 8 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Macromolecule #6: CALCIUM ION
Macromolecule | Name: CALCIUM ION / type: ligand / ID: 6 / Number of copies: 4 / Formula: CA |
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Molecular weight | Theoretical: 40.078 Da |
-Macromolecule #7: water
Macromolecule | Name: water / type: ligand / ID: 7 / Number of copies: 789 / Formula: HOH |
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Molecular weight | Theoretical: 18.015 Da |
Chemical component information | ![]() ChemComp-HOH: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.2 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |