EMDB-60139: Human 26S immunoproteasome

Basic information

Entry

Database: EMDB / ID: EMD-60139

• Title: Human 26S immunoproteasome
• Map data: Locally filtered, globally sharpened map using a local resolution estimation computed from the half-maps.

Sample

• Complex: Human 26S immunoproteasome
  • Protein or peptide: x 33 types

• Keywords: proteasome / immunoproteasome / HYDROLASE
• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 3.6 Å
• Authors: Saratov GA / Baymukhametov TN / Konevega AL / Kudriaeva AA / Belogurov AA

Funding support

Russian Federation, 1 items

Organization	Grant number	Country
Russian Science Foundation	23-74-00053	Russian Federation

• Citation: Journal: Russ.J.Bioorganic Chem. / Year: 2024; Title: The 3.6 Angstroms cryo-EM structure of the outer heptameric alpha-ring of human 26S immunoproteasome in the preactivation state.; Authors: Saratov GA / Baymukhametov TN / Konevega AL / Kudriaeva AA / Belogurov AA

History

Deposition	May 14, 2024	-
Header (metadata) release	Jun 19, 2024	-
Map release	Jun 19, 2024	-
Update	Jun 19, 2024	-
Current status	Jun 19, 2024	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_60139.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Locally filtered, globally sharpened map using a local resolution estimation computed from the half-maps.
• Voxel size: X=Y=Z: 1.8 Å

Density

Contour Level	By AUTHOR: 0.08
Minimum - Maximum	-0.17556575 - 0.4256472
Average (Standard dev.)	0.00067605195 (±0.008018609)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 720.0 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_60139_msk_1.map

Additional map: The map post-processed by deepEMhancer using a default...

• File: emd_60139_additional_1.map
• Annotation: The map post-processed by deepEMhancer using a default deep learning model 'tightTarget' (with mask).

Additional map: The map post-processed by deepEMhancer using a default...

• File: emd_60139_additional_2.map
• Annotation: The map post-processed by deepEMhancer using a default deep learning model 'tightTarget' (without mask).

Additional map: Unfiltered and unsharpened map obtained using cryoSPARC's 3D...

• File: emd_60139_additional_3.map
• Annotation: Unfiltered and unsharpened map obtained using cryoSPARC's 3D flexible refinement.

Half map: The half-map masked by a relatively wide (15...

• File: emd_60139_half_map_1.map
• Annotation: The half-map masked by a relatively wide (15 angstrom dilation in a 256 px box) solvent soft mask due to the peculiarities of the cryoSPARC's 3D Flex Reconstruct procedure.

Half map: The half-map masked by a relatively wide (15...

• File: emd_60139_half_map_2.map
• Annotation: The half-map masked by a relatively wide (15 angstrom dilation in a 256 px box) solvent soft mask due to the peculiarities of the cryoSPARC's 3D Flex Reconstruct procedure.

Sample components

Entire : Human 26S immunoproteasome

• Entire: Name: Human 26S immunoproteasome

Components

• Complex: Human 26S immunoproteasome
  • Protein or peptide: Proteasome subunit alpha type-1
  • Protein or peptide: Proteasome subunit alpha type-2
  • Protein or peptide: Proteasome subunit alpha type-3
  • Protein or peptide: Proteasome subunit alpha type-4
  • Protein or peptide: Proteasome subunit alpha type-5
  • Protein or peptide: Proteasome subunit alpha type-6
  • Protein or peptide: Proteasome subunit alpha type-7
  • Protein or peptide: Proteasome subunit beta type-1
  • Protein or peptide: Proteasome subunit beta type-2
  • Protein or peptide: Proteasome subunit beta type-3
  • Protein or peptide: Proteasome subunit beta type-4
  • Protein or peptide: Proteasome subunit beta type-8
  • Protein or peptide: Proteasome subunit beta type-9
  • Protein or peptide: Proteasome subunit beta type-10
  • Protein or peptide: 26S proteasome regulatory subunit 4
  • Protein or peptide: 26S proteasome regulatory subunit 6A
  • Protein or peptide: 26S proteasome regulatory subunit 6B
  • Protein or peptide: 26S proteasome regulatory subunit 7
  • Protein or peptide: 26S proteasome regulatory subunit 8
  • Protein or peptide: 26S proteasome regulatory subunit 10B
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 1
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 2
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 3
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 4
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 6
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 7
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 8
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 9
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 11
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 12
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 13
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 14 (His)x6 TEV protease site
  • Protein or peptide: Proteasomal ubiquitin receptor ADRM1

Supramolecule #1: Human 26S immunoproteasome

• Supramolecule: Name: Human 26S immunoproteasome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
• Source (natural): Organism: Homo sapiens (human) / Strain: HeLa / Organ: Cervix
• Molecular weight: Theoretical: 2.5 MDa

Macromolecule #1: Proteasome subunit alpha type-1

• Macromolecule: Name: Proteasome subunit alpha type-1 / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MFRNQYDNDV TVWSPQGRIH QIEYAMEAVK QGSATVGLKS KTHAVLVALK RAQSELAAHQ KKILHVDNHI GISIAGLTAD ARLLCNFMRQ ECLDSRFVFD RPLPVSRLVS LIGSKTQIPT QRYGRRPYGV GLLIAGYDDM GPHIFQTCPS ANYFDCRAMS IGARSQSART YLERHMSEFM ECNLNELVKH GLRALRETLP AEQDLTTKNV SIGIVGKDLE FTIYDDDDVS PFLEGLEERP QRKAQPAQPA DEPAEKADEP MEH

Macromolecule #2: Proteasome subunit alpha type-2

• Macromolecule: Name: Proteasome subunit alpha type-2 / type: protein_or_peptide / ID: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MAERGYSFSL TTFSPSGKLV QIEYALAAVA GGAPSVGIKA ANGVVLATEK KQKSILYDER SVHKVEPITK HIGLVYSGMG PDYRVLVHRA RKLAQQYYLV YQEPIPTAQL VQRVASVMQE YTQSGGVRPF GVSLLICGWN EGRPYLFQSD PSGAYFAWKA TAMGKNYVNG KTFLEKRYNE DLELEDAIHT AILTLKESFE GQMTEDNIEV GICNEAGFRR LTPTEVKDYL AAIA

Macromolecule #3: Proteasome subunit alpha type-3

• Macromolecule: Name: Proteasome subunit alpha type-3 / type: protein_or_peptide / ID: 3 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MSSIGTGYDL SASTFSPDGR VFQVEYAMKA VENSSTAIGI RCKDGVVFGV EKLVLSKLYE EGSNKRLFNV DRHVGMAVAG LLADARSLAD IAREEASNFR SNFGYNIPLK HLADRVAMYV HAYTLYSAVR PFGCSFMLGS YSVNDGAQLY MIDPSGVSYG YWGCAIGKAR QAAKTEIEKL QMKEMTCRDI VKEVAKIIYI VHDEVKDKAF ELELSWVGEL TNGRHEIVPK DIREEAEKYA KESLKEEDES DDDNM

Macromolecule #4: Proteasome subunit alpha type-4

• Macromolecule: Name: Proteasome subunit alpha type-4 / type: protein_or_peptide / ID: 4 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MSRRYDSRTT IFSPEGRLYQ VEYAMEAIGH AGTCLGILAN DGVLLAAERR NIHKLLDEVF FSEKIYKLNE DMACSVAGIT SDANVLTNEL RLIAQRYLLQ YQEPIPCEQL VTALCDIKQA YTQFGGKRPF GVSLLYIGWD KHYGFQLYQS DPSGNYGGWK ATCIGNNSAA AVSMLKQDYK EGEMTLKSAL ALAIKVLNKT MDVSKLSAEK VEIATLTREN GKTVIRVLKQ KEVEQLIKKH EEEEAKAERE KKEKEQKEKD K

Macromolecule #5: Proteasome subunit alpha type-5

• Macromolecule: Name: Proteasome subunit alpha type-5 / type: protein_or_peptide / ID: 5 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MFLTRSEYDR GVNTFSPEGR LFQVEYAIEA IKLGSTAIGI QTSEGVCLAV EKRITSPLME PSSIEKIVEI DAHIGCAMSG LIADAKTLID KARVETQNHW FTYNETMTVE SVTQAVSNLA LQFGEEDADP GAMSRPFGVA LLFGGVDEKG PQLFHMDPSG TFVQCDARAI GSASEGAQSS LQEVYHKSMT LKEAIKSSLI ILKQVMEEKL NATNIELATV QPGQNFHMFT KEELEEVIKD I

Macromolecule #6: Proteasome subunit alpha type-6

• Macromolecule: Name: Proteasome subunit alpha type-6 / type: protein_or_peptide / ID: 6 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MSRGSSAGFD RHITIFSPEG RLYQVEYAFK AINQGGLTSV AVRGKDCAVI VTQKKVPDKL LDSSTVTHLF KITENIGCVM TGMTADSRSQ VQRARYEAAN WKYKYGYEIP VDMLCKRIAD ISQVYTQNAE MRPLGCCMIL IGIDEEQGPQ VYKCDPAGYY CGFKATAAGV KQTESTSFLE KKVKKKFDWT FEQTVETAIT CLSTVLSIDF KPSEIEVGVV TVENPKFRIL TEAEIDAHLV ALAERD

Macromolecule #7: Proteasome subunit alpha type-7

• Macromolecule: Name: Proteasome subunit alpha type-7 / type: protein_or_peptide / ID: 7 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MSYDRAITVF SPDGHLFQVE YAQEAVKKGS TAVGVRGRDI VVLGVEKKSV AKLQDERTVR KICALDDNVC MAFAGLTADA RIVINRARVE CQSHRLTVED PVTVEYITRY IASLKQRYTQ SNGRRPFGIS ALIVGFDFDG TPRLYQTDPS GTYHAWKANA IGRGAKSVRE FLEKNYTDEA IETDDLTIKL VIKALLEVVQ SGGKNIELAV MRRDQSLKIL NPEEIEKYVA EIEKEKEENE KKKQKKAS

Macromolecule #8: Proteasome subunit beta type-1

• Macromolecule: Name: Proteasome subunit beta type-1 / type: protein_or_peptide / ID: 8 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MLSSTAMYSA PGRDLGMEPH RAAGPLQLRF SPYVFNGGTI LAIAGEDFAI VASDTRLSEG FSIHTRDSPK CYKLTDKTVI GCSGFHGDCL TLTKIIEARL KMYKHSNNKA MTTGAIAAML STILYSRRFF PYYVYNIIGG LDEEGKGAVY SFDPVGSYQR DSFKAGGSAS AMLQPLLDNQ VGFKNMQNVE HVPLSLDRAM RLVKDVFISA AERDVYTGDA LRICIVTKEG IREETVSLRK D

Macromolecule #9: Proteasome subunit beta type-2

• Macromolecule: Name: Proteasome subunit beta type-2 / type: protein_or_peptide / ID: 9 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLAD CLRSRTPYHV NLLLAGYDEH EGPALYYMDY LAALAKAPFA AHGYGAFLTL SILDRYYTPT ISRERAVELL RKCLEELQKR FILNLPTFSV RIIDKNGIHD LDNISFPKQG S

Macromolecule #10: Proteasome subunit beta type-3

• Macromolecule: Name: Proteasome subunit beta type-3 / type: protein_or_peptide / ID: 10 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MSIMSYNGGA VMAMKGKNCV AIAADRRFGI QAQMVTTDFQ KIFPMGDRLY IGLAGLATDV QTVAQRLKFR LNLYELKEGR QIKPYTLMSM VANLLYEKRF GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCAEQMYGMC ESLWEPNMDP DHLFETISQA MLNAVDRDAV SGMGVIVHII EKDKITTRTL KARMD

Macromolecule #11: Proteasome subunit beta type-4

• Macromolecule: Name: Proteasome subunit beta type-4 / type: protein_or_peptide / ID: 11 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MEAFLGSRSG LWAGGPAPGQ FYRIPSTPDS FMDPASALYR GPITRTQNPM VTGTSVLGVK FEGGVVIAAD MLGSYGSLAR FRNISRIMRV NNSTMLGASG DYADFQYLKQ VLGQMVIDEE LLGDGHSYSP RAIHSWLTRA MYSRRSKMNP LWNTMVIGGY ADGESFLGYV DMLGVAYEAP SLATGYGAYL AQPLLREVLE KQPVLSQTEA RDLVERCMRV LYYRDARSYN RFQIATVTEK GVEIEGPLST ETNWDIAHMI SGFE

Macromolecule #12: Proteasome subunit beta type-8

• Macromolecule: Name: Proteasome subunit beta type-8 / type: protein_or_peptide / ID: 12 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MALLDVCGAP RGQRPESALP VAGSGRRSDP GHYSFSMRSP ELALPRGMQP TEFFQSLGGD GERNVQIEMA HGTTTLAFKF QHGVIAAVDS RASAGSYISA LRVNKVIEIN PYLLGTMSGC AADCQYWERL LAKECRLYYL RNGERISVSA ASKLLSNMMC QYRGMGLSMG SMICGWDKKG PGLYYVDEHG TRLSGNMFST GSGNTYAYGV MDSGYRPNLS PEEAYDLGRR AIAYATHRDS YSGGVVNMYH MKEDGWVKVE STDVSDLLHQ YREANQ

Macromolecule #13: Proteasome subunit beta type-9

• Macromolecule: Name: Proteasome subunit beta type-9 / type: protein_or_peptide / ID: 13 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MLRAGAPTGD LPRAGEVHTG TTIMAVEFDG GVVMGSDSRV SAGEAVVNRV FDKLSPLHER IYCALSGSAA DAQAVADMAA YQLELHGIEL EEPPLVLAAA NVVRNISYKY REDLSAHLMV AGWDQREGGQ VYGTLGGMLT RQPFAIGGSG STFIYGYVDA AYKPGMSPEE CRRFTTDAIA LAMSRDGSSG GVIYLVTITA AGVDHRVILG NELPKFYDE

Macromolecule #14: Proteasome subunit beta type-10

• Macromolecule: Name: Proteasome subunit beta type-10 / type: protein_or_peptide / ID: 14 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MLKPALEPRG GFSFENCQRN ASLERVLPGL KVPHARKTGT TIAGLVFQDG VILGADTRAT NDSVVADKSC EKIHFIAPKI YCCGAGVAAD AEMTTRMVAS KMELHALSTG REPRVATVTR ILRQTLFRYQ GHVGASLIVG GVDLTGPQLY GVHPHGSYSR LPFTALGSGQ DAALAVLEDR FQPNMTLEAA QGLLVEAVTA GILGDLGSGG NVDACVITKT GAKLLRTLSS PTEPVKRSGR YHFVPGTTAV LTQTVKPLTL ELVEETVQAM EVE

Macromolecule #15: 26S proteasome regulatory subunit 4

• Macromolecule: Name: 26S proteasome regulatory subunit 4 / type: protein_or_peptide / ID: 15 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MGQSQSGGHG PGGGKKDDKD KKKKYEPPVP TRVGKKKKKT KGPDAASKLP LVTPHTQCRL KLLKLERIKD YLLMEEEFIR NQEQMKPLEE KQEEERSKVD DLRGTPMSVG TLEEIIDDNH AIVSTSVGSE HYVSILSFVD KDLLEPGCSV LLNHKVHAVI GVLMDDTDPL VTVMKVEKAP QETYADIGGL DNQIQEIKES VELPLTHPEY YEEMGIKPPK GVILYGPPGT GKTLLAKAVA NQTSATFLRV VGSELIQKYL GDGPKLVREL FRVAEEHAPS IVFIDEIDAI GTKRYDSNSG GEREIQRTML ELLNQLDGFD SRGDVKVIMA TNRIETLDPA LIRPGRIDRK IEFPLPDEKT KKRIFQIHTS RMTLADDVTL DDLIMAKDDL SGADIKAICT EAGLMALRER RMKVTNEDFK KSKENVLYKK QEGTPEGLYL

Macromolecule #16: 26S proteasome regulatory subunit 6A

• Macromolecule: Name: 26S proteasome regulatory subunit 6A / type: protein_or_peptide / ID: 16 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MNLLPNIESP VTRQEKMATV WDEAEQDGIG EEVLKMSTEE IIQRTRLLDS EIKIMKSEVL RVTHELQAMK DKIKENSEKI KVNKTLPYLV SNVIELLDVD PNDQEEDGAN IDLDSQRKGK CAVIKTSTRQ TYFLPVIGLV DAEKLKPGDL VGVNKDSYLI LETLPTEYDS RVKAMEVDER PTEQYSDIGG LDKQIQELVE AIVLPMNHKE KFENLGIQPP KGVLMYGPPG TGKTLLARAC AAQTKATFLK LAGPQLVQMF IGDGAKLVRD AFALAKEKAP SIIFIDELDA IGTKRFDSEK AGDREVQRTM LELLNQLDGF QPNTQVKVIA ATNRVDILDP ALLRSGRLDR KIEFPMPNEE ARARIMQIHS RKMNVSPDVN YEELARCTDD FNGAQCKAVC VEAGMIALRR GATELTHEDY MEGILEVQAK KKANLQYYA

Macromolecule #17: 26S proteasome regulatory subunit 6B

• Macromolecule: Name: 26S proteasome regulatory subunit 6B / type: protein_or_peptide / ID: 17 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MEEIGILVEK AQDEIPALSV SRPQTGLSFL GPEPEDLEDL YSRYKKLQQE LEFLEVQEEY IKDEQKNLKK EFLHAQEEVK RIQSIPLVIG QFLEAVDQNT AIVGSTTGSN YYVRILSTID RELLKPNASV ALHKHSNALV DVLPPEADSS IMMLTSDQKP DVMYADIGGM DIQKQEVREA VELPLTHFEL YKQIGIDPPR GVLMYGPPGC GKTMLAKAVA HHTTAAFIRV VGSEFVQKYL GEGPRMVRDV FRLAKENAPA IIFIDEIDAI ATKRFDAQTG ADREVQRILL ELLNQMDGFD QNVNVKVIMA TNRADTLDPA LLRPGRLDRK IEFPLPDRRQ KRLIFSTITS KMNLSEEVDL EDYVARPDKI SGADINSICQ ESGMLAVREN RYIVLAKDFE KAYKTVIKKD EQEHEFYK

Macromolecule #18: 26S proteasome regulatory subunit 7

• Macromolecule: Name: 26S proteasome regulatory subunit 7 / type: protein_or_peptide / ID: 18 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MPDYLGADQR KTKEDEKDDK PIRALDEGDI ALLKTYGQST YSRQIKQVED DIQQLLKKIN ELTGIKESDT GLAPPALWDL AADKQTLQSE QPLQVARCTK IINADSEDPK YIINVKQFAK FVVDLSDQVA PTDIEEGMRV GVDRNKYQIH IPLPPKIDPT VTMMQVEEKP DVTYSDVGGC KEQIEKLREV VETPLLHPER FVNLGIEPPK GVLLFGPPGT GKTLCARAVA NRTDACFIRV IGSELVQKYV GEGARMVREL FEMARTKKAC LIFFDEIDAI GGARFDDGAG GDNEVQRTML ELINQLDGFD PRGNIKVLMA TNRPDTLDPA LMRPGRLDRK IEFSLPDLEG RTHIFKIHAR SMSVERDIRF ELLARLCPNS TGAEIRSVCT EAGMFAIRAR RKIATEKDFL EAVNKVIKSY AKFSATPRYM TYN

Macromolecule #19: 26S proteasome regulatory subunit 8

• Macromolecule: Name: 26S proteasome regulatory subunit 8 / type: protein_or_peptide / ID: 19 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MALDGPEQME LEEGKAGSGL RQYYLSKIEE LQLIVNDKSQ NLRRLQAQRN ELNAKVRLLR EELQLLQEQG SYVGEVVRAM DKKKVLVKVH PEGKFVVDVD KNIDINDVTP NCRVALRNDS YTLHKILPNK VDPLVSLMMV EKVPDSTYEM IGGLDKQIKE IKEVIELPVK HPELFEALGI AQPKGVLLYG PPGTGKTLLA RAVAHHTDCT FIRVSGSELV QKFIGEGARM VRELFVMARE HAPSIIFMDE IDSIGSSRLE GGSGGDSEVQ RTMLELLNQL DGFEATKNIK VIMATNRIDI LDSALLRPGR IDRKIEFPPP NEEARLDILK IHSRKMNLTR GINLRKIAEL MPGASGAEVK GVCTEAGMYA LRERRVHVTQ EDFEMAVAKV MQKDSEKNMS IKKLWK

Macromolecule #20: 26S proteasome regulatory subunit 10B

• Macromolecule: Name: 26S proteasome regulatory subunit 10B / type: protein_or_peptide / ID: 20 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MADPRDKALQ DYRKKLLEHK EIDGRLKELR EQLKELTKQY EKSENDLKAL QSVGQIVGEV LKQLTEEKFI VKATNGPRYV VGCRRQLDKS KLKPGTRVAL DMTTLTIMRY LPREVDPLVY NMSHEDPGNV SYSEIGGLSE QIRELREVIE LPLTNPELFQ RVGIIPPKGC LLYGPPGTGK TLLARAVASQ LDCNFLKVVS SSIVDKYIGE SARLIREMFN YARDHQPCII FMDEIDAIGG RRFSEGTSAD REIQRTLMEL LNQMDGFDTL HRVKMIMATN RPDTLDPALL RPGRLDRKIH IDLPNEQARL DILKIHAGPI TKHGEIDYEA IVKLSDGFNG ADLRNVCTEA GMFAIRADHD FVVQEDFMKA VRKVADSKKL ESKLDYKPV

Macromolecule #21: 26S proteasome non-ATPase regulatory subunit 1

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 1 / type: protein_or_peptide / ID: 21 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MITSAAGIIS LLDEDEPQLK EFALHKLNAV VNDFWAEISE SVDKIEVLYE DEGFRSRQFA ALVASKVFYH LGAFEESLNY ALGAGDLFNV NDNSEYVETI IAKCIDHYTK QCVENADLPE GEKKPIDQRL EGIVNKMFQR CLDDHKYKQA IGIALETRRL DVFEKTILES NDVPGMLAYS LKLCMSLMQN KQFRNKVLRV LVKIYMNLEK PDFINVCQCL IFLDDPQAVS DILEKLVKED NLLMAYQICF DLYESASQQF LSSVIQNLRT VGTPIASVPG STNTGTVPGS EKDSDSMETE EKTSSAFVGK TPEASPEPKD QTLKMIKILS GEMAIELHLQ FLIRNNNTDL MILKNTKDAV RNSVCHTATV IANSFMHCGT TSDQFLRDNL EWLARATNWA KFTATASLGV IHKGHEKEAL QLMATYLPKD TSPGSAYQEG GGLYALGLIH ANHGGDIIDY LLNQLKNASN DIVRHGGSLG LGLAAMGTAR QDVYDLLKTN LYQDDAVTGE AAGLALGLVM LGSKNAQAIE DMVGYAQETQ HEKILRGLAV GIALVMYGRM EEADALIESL CRDKDPILRR SGMYTVAMAY CGSGNNKAIR RLLHVAVSDV NDDVRRAAVE SLGFILFRTP EQCPSVVSLL SESYNPHVRY GAAMALGICC AGTGNKEAIN LLEPMTNDPV NYVRQGALIA SALIMIQQTE ITCPKVNQFR QLYSKVINDK HDDVMAKFGA ILAQGILDAG GHNVTISLQS RTGHTHMPSV VGVLVFTQFW FWFPLSHFLS LAYTPTCVIG LNKDLKMPKV QYKSNCKPST FAYPAPLEVP KEKEKEKVST AVLSITAKAK KKEKEKEKKE EEKMEVDEAE KKEEKEKKKE PEPNFQLLDN PARVMPAQLK VLTMPETCRY QPFKPLSIGG IIILKDTSED IEELVEPVAA HGPKIEEEEQ EPEPPEPFEY IDD

Macromolecule #22: 26S proteasome non-ATPase regulatory subunit 2

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 2 / type: protein_or_peptide / ID: 22 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MEEGGRDKAP VQPQQSPAAA PGGTDEKPSG KERRDAGDKD KEQELSEEDK QLQDELEMLV ERLGEKDTSL YRPALEELRR QIRSSTTSMT SVPKPLKFLR PHYGKLKEIY ENMAPGENKR FAADIISVLA MTMSGERECL KYRLVGSQEE LASWGHEYVR HLAGEVAKEW QELDDAEKVQ REPLLTLVKE IVPYNMAHNA EHEACDLLME IEQVDMLEKD IDENAYAKVC LYLTSCVNYV PEPENSALLR CALGVFRKFS RFPEALRLAL MLNDMELVED IFTSCKDVVV QKQMAFMLGR HGVFLELSED VEEYEDLTEI MSNVQLNSNF LALARELDIM EPKVPDDIYK THLENNRFGG SGSQVDSARM NLASSFVNGF VNAAFGQDKL LTDDGNKWLY KNKDHGMLSA AASLGMILLW DVDGGLTQID KYLYSSEDYI KSGALLACGI VNSGVRNECD PALALLSDYV LHNSNTMRLG SIFGLGLAYA GSNREDVLTL LLPVMGDSKS SMEVAGVTAL ACGMIAVGSC NGDVTSTILQ TIMEKSETEL KDTYARWLPL GLGLNHLGKG EAIEAILAAL EVVSEPFRSF ANTLVDVCAY AGSGNVLKVQ QLLHICSEHF DSKEKEEDKD KKEKKDKDKK EAPADMGAHQ GVAVLGIALI AMGEEIGAEM ALRTFGHLLR YGEPTLRRAV PLALALISVS NPRLNILDTL SKFSHDADPE VSYNSIFAMG MVGSGTNNAR LAAMLRQLAQ YHAKDPNNLF MVRLAQGLTH LGKGTLTLCP YHSDRQLMSQ VAVAGLLTVL VSFLDVRNII LGKSHYVLYG LVAAMQPRML VTFDEELRPL PVSVRVGQAV DVVGQAGKPK TITGFQTHTT PVLLAHGERA ELATEEFLPV TPILEGFVIL RKNPNYDL

Macromolecule #23: 26S proteasome non-ATPase regulatory subunit 3

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 3 / type: protein_or_peptide / ID: 23 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MKQEGSARRR GADKAKPPPG GGEQEPPPPP APQDVEMKEE AATGGGSTGE ADGKTAAAAA EHSQRELDTV TLEDIKEHVK QLEKAVSGKE PRFVLRALRM LPSTSRRLNH YVLYKAVQGF FTSNNATRDF LLPFLEEPMD TEADLQFRPR TGKAASTPLL PEVEAYLQLL VVIFMMNSKR YKEAQKISDD LMQKISTQNR RALDLVAAKC YYYHARVYEF LDKLDVVRSF LHARLRTATL RHDADGQATL LNLLLRNYLH YSLYDQAEKL VSKSVFPEQA NNNEWARYLY YTGRIKAIQL EYSEARRTMT NALRKAPQHT AVGFKQTVHK LLIVVELLLG EIPDRLQFRQ PSLKRSLMPY FLLTQAVRTG NLAKFNQVLD QFGEKFQADG TYTLIIRLRH NVIKTGVRMI SLSYSRISLA DIAQKLQLDS PEDAEFIVAK AIRDGVIEAS INHEKGYVQS KEMIDIYSTR EPQLAFHQRI SFCLDIHNMS VKAMRFPPKS YNKDLESAEE RREREQQDLE FAKEMAEDDD DSFP

Macromolecule #24: 26S proteasome non-ATPase regulatory subunit 4

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 4 / type: protein_or_peptide / ID: 24 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MVLESTMVCV DNSEYMRNGD FLPTRLQAQQ DAVNIVCHSK TRSNPENNVG LITLANDCEV LTTLTPDTGR ILSKLHTVQP KGKITFCTGI RVAHLALKHR QGKNHKMRII AFVGSPVEDN EKDLVKLAKR LKKEKVNVDI INFGEEEVNT EKLTAFVNTL NGKDGTGSHL VTVPPGPSLA DALISSPILA GEGGAMLGLG ASDFEFGVDP SADPELALAL RVSMEEQRQR QEEEARRAAA ASAAEAGIAT TGTEDSDDAL LKMTISQQEF GRTGLPDLSS MTEEEQIAYA MQMSLQGAEF GQAESADIDA SSAMDTSEPA KEEDDYDVMQ DPEFLQSVLE NLPGVDPNNE AIRNAMGSLA SQATKDGKKD KKEEDKK

Macromolecule #25: 26S proteasome non-ATPase regulatory subunit 6

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 6 / type: protein_or_peptide / ID: 25 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MPLENLEEEG LPKNPDLRIA QLRFLLSLPE HRGDAAVRDE LMAAVRDNNM APYYEALCKS LDWQIDVDLL NKMKKANEDE LKRLDEELED AEKNLGESEI RDAMMAKAEY LCRIGDKEGA LTAFRKTYDK TVALGHRLDI VFYLLRIGLF YMDNDLITRN TEKAKSLIEE GGDWDRRNRL KVYQGLYCVA IRDFKQAAEL FLDTVSTFTS YELMDYKTFV TYTVYVSMIA LERPDLREKV IKGAEILEVL HSLPAVRQYL FSLYECRYSV FFQSLAVVEQ EMKKDWLFAP HYRYYVREMR IHAYSQLLES YRSLTLGYMA EAFGVGVEFI DQELSRFIAA GRLHCKIDKV NEIVETNRPD SKNWQYQETI KKGDLLLNRV QKLSRVINM

Macromolecule #26: 26S proteasome non-ATPase regulatory subunit 7

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 7 / type: protein_or_peptide / ID: 26 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MPELAVQKVV VHPLVLLSVV DHFNRIGKVG NQKRVVGVLL GSWQKKVLDV SNSFAVPFDE DDKDDSVWFL DHDYLENMYG MFKKVNARER IVGWYHTGPK LHKNDIAINE LMKRYCPNSV LVIIDVKPKD LGLPTEAYIS VEEVHDDGTP TSKTFEHVTS EIGAEEAEEV GVEHLLRDIK DTTVGTLSQR ITNQVHGLKG LNSKLLDIRS YLEKVATGKL PINHQIIYQL QDVFNLLPDV SLQEFVKAFY LKTNDQMVVV YLASLIRSVV ALHNLINNKI ANRDAEKKEG QEKEESKKDR KEDKEKDKDK EKSDVKKEEK KEKK

Macromolecule #27: 26S proteasome non-ATPase regulatory subunit 8

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 8 / type: protein_or_peptide / ID: 27 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MFIKGRAPRA PPRERRRATR GGLRQVVAPP RALGSTSRPH FRRASVCRRR CRKSGGLLAA SRKMAAAAVN GAAGFSSSGP AATSGAVLQA ATGMYEQLKG EWNRKSPNLS KCGEELGRLK LVLLELNFLP TTGTKLTKQQ LILARDILEI GAQWSILRKD IPSFERYMAQ LKCYYFDYKE QLPESAYMHQ LLGLNLLFLL SQNRVAEFHT ELERLPAKDI QTNVYIKHPV SLEQYLMEGS YNKVFLAKGN IPAESYTFFI DILLDTIRDE IAGCIEKAYE KILFTEATRI LFFNTPKKMT DYAKKRGWVL GPNNYYSFAS QQQKPEDTTI PSTELAKQVI EYARQLEMIV

Macromolecule #28: 26S proteasome non-ATPase regulatory subunit 9

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 9 / type: protein_or_peptide / ID: 28 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MSDEEARQSG GSSQAGVVTV SDVQELMRRK EEIEAQIKAN YDVLESQKGI GMNEPLVDCE GYPRSDVDLY QVRTARHNII CLQNDHKAVM KQVEEALHQL HARDKEKQAR DMAEAHKEAM SRKLGQSESQ GPPRAFAKVN SISPGSPASI AGLQVDDEIV EFGSVNTQNF QSLHNIGSVV QHSEGKPLNV TVIRRGEKHQ LRLVPTRWAG KGLLGCNIIP LQR

Macromolecule #29: 26S proteasome non-ATPase regulatory subunit 11

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 11 / type: protein_or_peptide / ID: 29 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MAAAAVVEFQ RAQSLLSTDR EASIDILHSI VKRDIQENDE EAVQVKEQSI LELGSLLAKT GQAAELGGLL KYVRPFLNSI SKAKAARLVR SLLDLFLDME AATGQEVELC LECIEWAKSE KRTFLRQALE ARLVSLYFDT KRYQEALHLG SQLLRELKKM DDKALLVEVQ LLESKTYHAL SNLPKARAAL TSARTTANAI YCPPKLQATL DMQSGIIHAA EEKDWKTAYS YFYEAFEGYD SIDSPKAITS LKYMLLCKIM LNTPEDVQAL VSGKLALRYA GRQTEALKCV AQASKNRSLA DFEKALTDYR AELRDDPIIS THLAKLYDNL LEQNLIRVIE PFSRVQIEHI SSLIKLSKAD VERKLSQMIL DKKFHGILDQ GEGVLIIFDE PPVDKTYEAA LETIQNMSKV VDSLYNKAKK LT

Macromolecule #30: 26S proteasome non-ATPase regulatory subunit 12

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 12 / type: protein_or_peptide / ID: 30 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MADGGSERAD GRIVKMEVDY SATVDQRLPE CAKLAKEGRL QEVIETLLSL EKQTRTASDM VSTSRILVAV VKMCYEAKEW DLLNENIMLL SKRRSQLKQA VAKMVQQCCT YVEEITDLPI KLRLIDTLRM VTEGKIYVEI ERARLTKTLA TIKEQNGDVK EAASILQELQ VETYGSMEKK ERVEFILEQM RLCLAVKDYI RTQIISKKIN TKFFQEENTE KLKLKYYNLM IQLDQHEGSY LSICKHYRAI YDTPCIQAES EKWQQALKSV VLYVILAPFD NEQSDLVHRI SGDKKLEEIP KYKDLLKLFT TMELMRWSTL VEDYGMELRK GSLESPATDV FGSTEEGEKR WKDLKNRVVE HNIRIMAKYY TRITMKRMAQ LLDLSVDESE AFLSNLVVNK TIFAKVDRLA GIINFQRPKD PNNLLNDWSQ KLNSLMSLVN KTTHLIAKEE MIHNLQ

Macromolecule #31: 26S proteasome non-ATPase regulatory subunit 13

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 13 / type: protein_or_peptide / ID: 31 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MKDVPGFLQQ SQNSGPGQPA VWHRLEELYT KKLWHQLTLQ VLDFVQDPCF AQGDGLIKLY ENFISEFEHR VNPLSLVEII LHVVRQMTDP NVALTFLEKT REKVKSSDEA VILCKTAIGA LKLNIGDLQV TKETIEDVEE MLNNLPGVTS VHSRFYDLSS KYYQTIGNHA SYYKDALRFL GCVDIKDLPV SEQQERAFTL GLAGLLGEGV FNFGELLMHP VLESLRNTDR QWLIDTLYAF NSGNVERFQT LKTAWGQQPD LAANEAQLLR KIQLLCLMEM TFTRPANHRQ LTFEEIAKSA KITVNEVELL VMKALSVGLV KGSIDEVDKR VHMTWVQPRV LDLQQIKGMK DRLEFWCTDV KSMEMLVEHQ AHDILT

Macromolecule #32: 26S proteasome non-ATPase regulatory subunit 14 (His)x6 TEV prote...

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 14 (His)x6 TEV protease site; type: protein_or_peptide / ID: 32 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MDRLLRLGGG MPGLGQGPPT DAPAVDTAEQ VYISSLALLK MLKHGRAGVP MEVMGLMLGE FVDDYTVRVI DVFAMPQSGT GVSVEAVDPV FQAKMLDMLK QTGRPEMVVG WYHSHPGFGC WLSGVDINTQ QSFEALSERA VAVVVDPIQS VKGKVVIDAF RLINANMMVL GHEPRQTTSN LGHLNKPSIQ ALIHGLNRHY YSITINYRKN ELEQKMLLNL HKKSWMEGLT LQDYSEHCKH NESVVKEMLE LAKNYNKAVE EEDKMTPEQL AIKNVGKQDP KRHLEEHVDV LMTSNIVQCL AAMLDTVVFK HHHHHHDYDI PTTASENLYF Q

Macromolecule #33: Proteasomal ubiquitin receptor ADRM1

• Macromolecule: Name: Proteasomal ubiquitin receptor ADRM1 / type: protein_or_peptide / ID: 33 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)

Sequence

String:

MTTSGALFPS LVPGSRGASN KYLVEFRAGK MSLKGTTVTP DKRKGLVYIQ QTDDSLIHFC WKDRTSGNVE DDLIIFPDDC EFKRVPQCPS GRVYVLKFKA GSKRLFFWMQ EPKTDQDEEH CRKVNEYLNN PPMPGALGAS GSSGHELSAL GGEGGLQSLL GNMSHSQLMQ LIGPAGLGGL GGLGALTGPG LASLLGSSGP PGSSSSSSSR SQSAAVTPSS TTSSTRATPA PSAPAAASAT SPSPAPSSGN GASTAASPTQ PIQLSDLQSI LATMNVPAGP AGGQQVDLAS VLTPEIMAPI LANADVQERL LPYLPSGESL PQTADEIQNT LTSPQFQQAL GMFSAALASG QLGPLMCQFG LPAEAVEAAN KGDVEAFAKA MQNNAKPEQK EGDTKDKKDE EEDMSLD

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.9 mg/mL
• Buffer: pH: 7.5 / Details: 30mM Tris-HCl, 5mM MgCl2, 1mM ATP, 1mM TCEP
• Grid: Model: Quantifoil R1.2/1.3 / Material: GRAPHENE OXIDE / Mesh: 400 / Support film - Material: GRAPHENE OXIDE / Support film - topology: HOLEY ARRAY / Details: The grid was not pretreated.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Specialist optics: Spherical aberration corrector: Microscope was modified with a Cs corrector (CEOS GmbH, Germany).
• Details: Preliminary grid screening was performed manually.
• Image recording: Film or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Digitization - Frames/image: 1-20 / Number grids imaged: 1 / Number real images: 1130 / Average exposure time: 3.0 sec. / Average electron dose: 60.0 e/Ų; Details: Images were collected in movie-mode at 20 frames per second.
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 100.0 µm / Calibrated magnification: 77778 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 0.01 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 37000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 146134
• Startup model: Type of model: OTHER / Details: Ab-initio reconstruction was performed.
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.2.1) / Number images used: 45782
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.2.1); Details: Heterogeneous Refinement was performed for the initial angular assignment of 20S, 26S and 30S particles.
• Final angle assignment: Type: OTHER / Software - Name: cryoSPARC (ver. 4.2.1); Details: 3D Flexible Refinement was performed for final reconstruction of 26S proteasome.