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- EMDB-60066: Cryo-EM structures of RNF168/UbcH5c-Ub in complex with H2AK13Ub n... -
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Open data
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Basic information
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Title | Cryo-EM structures of RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes (two Ub conformation) | |||||||||
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![]() | nucleosome / RNF168 / H2AK13/15 / H2AK13/15Ub / NUCLEAR PROTEIN | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.52 Å | |||||||||
![]() | Ai HS / Tong ZB / Deng ZH / Tian CL / Liu L / Pan M | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structures of RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes (two Ub conformation) Authors: Ai HS / Tong ZB / Deng ZH / Tian CL / Liu L / Pan M | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 3.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.3 KB 13.3 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 9.1 KB | Display | ![]() |
Images | ![]() | 121.9 KB | ||
Masks | ![]() | 64 MB | ![]() | |
Filedesc metadata | ![]() | 3.7 KB | ||
Others | ![]() ![]() ![]() | 49 MB 49.6 MB 49.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 714.6 KB | Display | ![]() |
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Full document | ![]() | 714.2 KB | Display | |
Data in XML | ![]() | 15.3 KB | Display | |
Data in CIF | ![]() | 20.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
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Links
EMDB pages | ![]() ![]() |
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Map
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.074 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Additional map: #1
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Projections & Slices |
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Density Histograms |
-Half map: #1
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Projections & Slices |
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Density Histograms |
-Half map: #2
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes
Entire | Name: RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes |
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Components |
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-Supramolecule #1: RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes
Supramolecule | Name: RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |