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Basic information

Entry
Database: EMDB / ID: EMD-58354
TitleUapA (Q408E) in MSP1E3D1 nanodiscs, Xanthine-bound state
Map dataUnsharpened map
Sample
  • Complex: UapA (Q408E) in MSP1E3D1 nanodiscs, the in xanthine-bound state.
    • Protein or peptide: Uric acid-xanthine permease
  • Ligand: MYRISTIC ACID
  • Ligand: ERGOSTEROL
  • Ligand: XANTHINE
KeywordsUapA / DDM / Transporter / Inward-facing / Uric Acid / Xanthine / MEMBRANE PROTEIN
Biological speciesAspergillus nidulans FGSC A4 (mold)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.48 Å
AuthorsBroutzakis G / Gatsogiannis C
Funding support Germany, 2 items
OrganizationGrant numberCountry
German Research Foundation (DFG)496113311 Germany
German Research Foundation (DFG)211/667-1 Germany
CitationJournal: To Be Published
Title: Cryo-EM of the eukaryotic purine transporter UapA demonstrates intramolecular and lipid regulation of transport
Authors: Broutzakis G / Pyrris Y / Akrani I / Neuhaus A / Mikros E / Diallinas G / Gatsogiannis C
History
DepositionJun 1, 2026-
Header (metadata) releaseJul 1, 2026-
Map releaseJul 1, 2026-
UpdateJul 1, 2026-
Current statusJul 1, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_58354.png

Downloads & links

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Map

FileReleased
AnnotationUnsharpened map
Voxel sizeX=Y=Z: 1.38 Å
Density
Contour LevelBy AUTHOR: 0.15
Minimum - Maximum-0.22600305 - 0.5559804
Average (Standard dev.)0.002011256 (±0.018659817)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions180180180
Spacing180180180
CellA=B=C: 248.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_58354_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : UapA (Q408E) in MSP1E3D1 nanodiscs, the in xanthine-bound state.

EntireName: UapA (Q408E) in MSP1E3D1 nanodiscs, the in xanthine-bound state.
Components
  • Complex: UapA (Q408E) in MSP1E3D1 nanodiscs, the in xanthine-bound state.
    • Protein or peptide: Uric acid-xanthine permease
  • Ligand: MYRISTIC ACID
  • Ligand: ERGOSTEROL
  • Ligand: XANTHINE

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Supramolecule #1: UapA (Q408E) in MSP1E3D1 nanodiscs, the in xanthine-bound state.

SupramoleculeName: UapA (Q408E) in MSP1E3D1 nanodiscs, the in xanthine-bound state.
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Aspergillus nidulans FGSC A4 (mold) / Strain: A4
Molecular weightTheoretical: 120 KDa

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Macromolecule #1: Uric acid-xanthine permease

MacromoleculeName: Uric acid-xanthine permease / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Aspergillus nidulans FGSC A4 (mold)
Molecular weightTheoretical: 55.353102 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: QRVRLLARHL TTREGLIGDY DYGFLFRPEL PFMKKDPRAP PFFGLNEKIP VLLAFILGLQ HALAMLAGVV TPPLIISSSL SLPSDLQQY LVSTSLIVCG LLSMVQITRF HIYKTPYYIG SGVLSVMGVS FSIISVASGA FNQMYSNGFC QLDEAGNRLP C PEAYGALI ...String:
QRVRLLARHL TTREGLIGDY DYGFLFRPEL PFMKKDPRAP PFFGLNEKIP VLLAFILGLQ HALAMLAGVV TPPLIISSSL SLPSDLQQY LVSTSLIVCG LLSMVQITRF HIYKTPYYIG SGVLSVMGVS FSIISVASGA FNQMYSNGFC QLDEAGNRLP C PEAYGALI GTSACCALVE ILLAFVPPKV IQKIFPPIVT GPTVMLIGIS LIGTGFKDWA GGSACMDDGM LCPSATAPRP LP WGSPEFI GLGFLVFVSI ILCERFGAPI MKSCSVVIGL LVGCIVAAAC GYFSHADIDA APAASFIWVK TFPLSVYGPM VLP IIAVFI ICACECIGDV TATCDVSRLE VRGGTFESRI QGAVLADGIN SVVAALATMT PMTTFAENNG VIALTRCANR WAGY CCCLI LIVAGIFAKF AAAIVAIPNS VMGGMKTFLF ASVVISGQAI VAKAPFTRRN RFILTASMAL GYGATLVPTW FGNVF PQTE NRDLEGFENA IELVLETGFA VTAFVAMLLN AIMPAE

UniProtKB: Uric acid-xanthine permease

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Macromolecule #2: MYRISTIC ACID

MacromoleculeName: MYRISTIC ACID / type: ligand / ID: 2 / Number of copies: 36 / Formula: MYR
Molecular weightTheoretical: 228.371 Da
Chemical component information

ChemComp-MYR:
MYRISTIC ACID

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Macromolecule #3: ERGOSTEROL

MacromoleculeName: ERGOSTEROL / type: ligand / ID: 3 / Number of copies: 10 / Formula: ERG
Molecular weightTheoretical: 396.648 Da
Chemical component information

ChemComp-ERG:
ERGOSTEROL

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Macromolecule #4: XANTHINE

MacromoleculeName: XANTHINE / type: ligand / ID: 4 / Number of copies: 2 / Formula: XAN
Molecular weightTheoretical: 152.111 Da
Chemical component information

ChemComp-XAN:
XANTHINE

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration5 mg/mL
BufferpH: 7.5
Component:
ConcentrationFormulaName
20.0 mMC4H11NO3Tris
150.0 mMNaClSodium Chloride
0.03 %C24H46O11DDM
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 286.15 K / Instrument: FEI VITROBOT MARK IV
Details: Vitrification chamber was allowed to equilibrate for 15 min at 100% humidity..

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.48 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.5.1) / Number images used: 54200
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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