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Yorodumi- EMDB-56831: E. coli 70S ribosome, conformational ground state mutation in SSU... -
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Open data
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Basic information
| Entry | ![]() | ||||||||||||||||||||||||||||||||||||
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| Title | E. coli 70S ribosome, conformational ground state mutation in SSU-h41, with A/P- and P/E-site tRNA | ||||||||||||||||||||||||||||||||||||
Map data | 70S main map | ||||||||||||||||||||||||||||||||||||
Sample |
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Keywords | 70S ribosome / RNA excited states / RIBOSOME | ||||||||||||||||||||||||||||||||||||
| Function / homology | Function and homology informationnegative regulation of cytoplasmic translational initiation / transcription antitermination factor activity, RNA binding / ornithine decarboxylase inhibitor activity / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / positive regulation of ribosome biogenesis / RNA-binding transcription regulator activity ...negative regulation of cytoplasmic translational initiation / transcription antitermination factor activity, RNA binding / ornithine decarboxylase inhibitor activity / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / positive regulation of ribosome biogenesis / RNA-binding transcription regulator activity / four-way junction DNA binding / negative regulation of cytoplasmic translation / DnaA-L2 complex / regulation of mRNA stability / translation repressor activity / negative regulation of translational initiation / negative regulation of DNA-templated DNA replication initiation / mRNA regulatory element binding translation repressor activity / positive regulation of RNA splicing / regulation of DNA-templated transcription elongation / transcription elongation factor complex / response to reactive oxygen species / cytosolic ribosome assembly / ribosome assembly / assembly of large subunit precursor of preribosome / transcription antitermination / DNA endonuclease activity / translational initiation / regulation of cell growth / DNA-templated transcription termination / response to radiation / maintenance of translational fidelity / mRNA 5'-UTR binding / regulation of translation / large ribosomal subunit / ribosomal small subunit assembly / transferase activity / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / 5S rRNA binding / small ribosomal subunit / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / response to antibiotic / negative regulation of DNA-templated transcription / hydrolase activity / mRNA binding / DNA binding / RNA binding / zinc ion binding / membrane / cytoplasm / cytosol Similarity search - Function | ||||||||||||||||||||||||||||||||||||
| Biological species | ![]() | ||||||||||||||||||||||||||||||||||||
| Method | single particle reconstruction / cryo EM / Resolution: 2.5 Å | ||||||||||||||||||||||||||||||||||||
Authors | Steinmetzger C / Riad M / Petzold K | ||||||||||||||||||||||||||||||||||||
| Funding support | European Union, Sweden, 11 items
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Citation | Journal: Biorxiv / Year: 2026Title: Universal 3D Motif dynamics in RNA: The A-minor Switch Authors: Steinmetzger C / Karlsson H / Fontana C / Galindo Riera N / Riad M / Kosek D / Schlagnitweit J / Steiner E / Friebe Sandoz S / Andersson ER / Petzold K | ||||||||||||||||||||||||||||||||||||
| History |
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Structure visualization
| Supplemental images |
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Downloads & links
-EMDB archive
| Map data | emd_56831.map.gz | 365.1 MB | EMDB map data format | |
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| Header (meta data) | emd-56831-v30.xml emd-56831.xml | 83.4 KB 83.4 KB | Display Display | EMDB header |
| FSC (resolution estimation) | emd_56831_fsc.xml | 21.5 KB | Display | FSC data file |
| Images | emd_56831.png | 215.3 KB | ||
| Masks | emd_56831_msk_1.map | 729 MB | Mask map | |
| Filedesc metadata | emd-56831.cif.gz | 15.7 KB | ||
| Others | emd_56831_additional_1.map.gz emd_56831_half_map_1.map.gz emd_56831_half_map_2.map.gz | 58.4 MB 677.4 MB 677.4 MB | ||
| Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-56831 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-56831 | HTTPS FTP |
-Related structure data
| Related structure data | ![]() 28ulMC ![]() 28uhC ![]() 28uiC ![]() 28ujC ![]() 28ukC ![]() 28umC ![]() 28unC ![]() 9tmiC ![]() 9tnlC ![]() 9tqaC M: atomic model generated by this map C: citing same article ( |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
| EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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| Related items in Molecule of the Month |
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Map
| File | Download / File: emd_56831.map.gz / Format: CCP4 / Size: 729 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| Annotation | 70S main map | ||||||||||||||||||||||||||||||||||||
| Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
| Voxel size | X=Y=Z: 0.7575 Å | ||||||||||||||||||||||||||||||||||||
| Density |
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| Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
| Details | EMDB XML:
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-Supplemental data
-Mask #1
| File | emd_56831_msk_1.map | ||||||||||||
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| Density Histograms |
-Additional map: 70S map filtered to local resolution
| File | emd_56831_additional_1.map | ||||||||||||
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| Annotation | 70S map filtered to local resolution | ||||||||||||
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| Density Histograms |
-Half map: 70S half map A
| File | emd_56831_half_map_1.map | ||||||||||||
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| Annotation | 70S half map A | ||||||||||||
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-Half map: 70S half map B
| File | emd_56831_half_map_2.map | ||||||||||||
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| Annotation | 70S half map B | ||||||||||||
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| Density Histograms |
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Sample components
+Entire : E. coli 70S ribosome
+Supramolecule #1: E. coli 70S ribosome
+Macromolecule #1: Large ribosomal subunit protein bL36A
+Macromolecule #2: Large ribosomal subunit protein bL31
+Macromolecule #3: Small ribosomal subunit protein uS11
+Macromolecule #4: Small ribosomal subunit protein uS12
+Macromolecule #6: Large ribosomal subunit protein uL2
+Macromolecule #7: Large ribosomal subunit protein uL3
+Macromolecule #8: Large ribosomal subunit protein uL15
+Macromolecule #9: Large ribosomal subunit protein uL16
+Macromolecule #10: Large ribosomal subunit protein bL17
+Macromolecule #11: Large ribosomal subunit protein bL32
+Macromolecule #16: Large ribosomal subunit protein bL33
+Macromolecule #17: Large ribosomal subunit protein bL34
+Macromolecule #18: Large ribosomal subunit protein bL35
+Macromolecule #19: Small ribosomal subunit protein uS2
+Macromolecule #20: Small ribosomal subunit protein uS3
+Macromolecule #21: Small ribosomal subunit protein uS4
+Macromolecule #22: Small ribosomal subunit protein uS5
+Macromolecule #23: Small ribosomal subunit protein bS6, fully modified isoform
+Macromolecule #24: Small ribosomal subunit protein uS7
+Macromolecule #25: Small ribosomal subunit protein uS8
+Macromolecule #26: Small ribosomal subunit protein uS9
+Macromolecule #27: Small ribosomal subunit protein uS10
+Macromolecule #28: Small ribosomal subunit protein uS13
+Macromolecule #29: Small ribosomal subunit protein uS14
+Macromolecule #30: Small ribosomal subunit protein uS15
+Macromolecule #31: Small ribosomal subunit protein bS16
+Macromolecule #32: Small ribosomal subunit protein uS17
+Macromolecule #33: Small ribosomal subunit protein bS18
+Macromolecule #34: Small ribosomal subunit protein uS19
+Macromolecule #35: Small ribosomal subunit protein bS20
+Macromolecule #36: Small ribosomal subunit protein bS21
+Macromolecule #38: Large ribosomal subunit protein uL4
+Macromolecule #39: Large ribosomal subunit protein uL5
+Macromolecule #40: Large ribosomal subunit protein uL6
+Macromolecule #41: Large ribosomal subunit protein bL9
+Macromolecule #42: Large ribosomal subunit protein uL13
+Macromolecule #43: Large ribosomal subunit protein uL14
+Macromolecule #44: Large ribosomal subunit protein uL18
+Macromolecule #45: Large ribosomal subunit protein bL19
+Macromolecule #46: Large ribosomal subunit protein bL20
+Macromolecule #47: Large ribosomal subunit protein bL21
+Macromolecule #48: Large ribosomal subunit protein uL22
+Macromolecule #49: Large ribosomal subunit protein uL23
+Macromolecule #50: Large ribosomal subunit protein uL24
+Macromolecule #51: Large ribosomal subunit protein bL25
+Macromolecule #52: Large ribosomal subunit protein bL27
+Macromolecule #53: Large ribosomal subunit protein bL28
+Macromolecule #54: Large ribosomal subunit protein uL29
+Macromolecule #55: Large ribosomal subunit protein uL30
+Macromolecule #5: 5S rRNA
+Macromolecule #12: 16S rRNA
+Macromolecule #13: A/P-site Val-tRNA^Val
+Macromolecule #14: P/E-site tRNA^fMet
+Macromolecule #15: 23S rRNA
+Macromolecule #37: mRNA
+Macromolecule #56: ZINC ION
+Macromolecule #57: MAGNESIUM ION
+Macromolecule #58: POTASSIUM ION
+Macromolecule #59: SPECTINOMYCIN
+Macromolecule #60: VALINE
+Macromolecule #61: water
-Experimental details
-Structure determination
| Method | cryo EM |
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Processing | single particle reconstruction |
| Aggregation state | particle |
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Sample preparation
| Buffer | pH: 7.6 |
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| Grid | Model: Quantifoil R2/2 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 120 sec. |
| Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
| Microscope | TFS KRIOS |
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| Software | Name: EPU |
| Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 49.88 e/Å2 |
| Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
| Electron optics | C2 aperture diameter: 20.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.3 µm / Nominal magnification: 165000 |
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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About Yorodumi



Keywords
Authors
Sweden, 11 items
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Processing
FIELD EMISSION GUN


