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- EMDB-56668: Cryo-ET of IAV (WSN-M1-Udorn) unfractionated -

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Basic information

Entry
Database: EMDB / ID: EMD-56668
TitleCryo-ET of IAV (WSN-M1-Udorn) unfractionated
Map dataCryo-ET of IAV (WSN-M1-Udorn) unfractionated
Sample
  • Virus: Influenza A virus (A/(H1N1))
KeywordsInfluenza A virus / cryo-ET / VIRUS
Biological speciesInfluenza A virus (A/(H1N1))
Methodelectron tomography / cryo EM
AuthorsPeterl S / Chlanda P
Funding support Germany, 1 items
OrganizationGrant numberCountry
German Research Foundation (DFG)240245660-SFB1129 Germany
CitationJournal: Nat Commun / Year: 2026
Title: Filamentous morphology of influenza A virus confers enhanced stability in aerosols.
Authors: Lu Liu / Ghislain Motos / Céline Terrettaz / Sarah Peterl / Josephine von Kempis / Marie O Pohl / Umut Karakus / Elisabeth Gaggioli / Beiping Luo / Ulrich K Krieger / Thomas Peter / Petr ...Authors: Lu Liu / Ghislain Motos / Céline Terrettaz / Sarah Peterl / Josephine von Kempis / Marie O Pohl / Umut Karakus / Elisabeth Gaggioli / Beiping Luo / Ulrich K Krieger / Thomas Peter / Petr Chlanda / Tamar Kohn / Athanasios Nenes / Silke Stertz /
Abstract: Airborne transmission of influenza A virus (IAV) poses significant challenges to public health. However, the mechanisms governing viral inactivation in aerosols remain poorly understood. IAVs exhibit ...Airborne transmission of influenza A virus (IAV) poses significant challenges to public health. However, the mechanisms governing viral inactivation in aerosols remain poorly understood. IAVs exhibit morphological variability, ranging from 100 nm spherical virions to micrometer-long filaments, depending on strain and growth conditions. Although virion morphology was shown to influence transmissibility, the mechanisms of how morphology affects airborne transmission are yet to be delineated. Here, we investigated the impact of virion shape on IAV stability in bulk solutions and an aerosol system with a focus on particles in the submicrometer range to examine how physicochemical aerosol properties, such as elevated solute concentration and low pH, affect infectivity. We show that filamentous viruses exhibit enhanced stability in aerosol particles at 80% relative humidity (RH) and in bulk solution mimicking increased salinity at this RH. Similarly, filamentous viruses exhibited slower decay under acidic conditions, both in bulk solutions and in acidified aerosol particles. Using primary human airway cultures, we further found that filamentous IAVs possess an infectivity advantage under mucosal immune pressures, including neutralizing antibodies and mucus. These results reveal that filamentous shape provides IAV with enhanced stability under diverse environmental conditions in the aerosol phase and increased infectivity in the respiratory epithelium.
History
DepositionFeb 10, 2026-
Header (metadata) releaseMay 27, 2026-
Map releaseMay 27, 2026-
UpdateMay 27, 2026-
Current statusMay 27, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_56668.png

Downloads & links

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Map

FileDownload / File: emd_56668.map.gz / Format: CCP4 / Size: 224.8 MB / Type: IMAGE STORED AS SIGNED BYTE
AnnotationCryo-ET of IAV (WSN-M1-Udorn) unfractionated
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
10.36 Å/pix.
x 160 pix.
= 1656.96 Å		10.36 Å/pix.
x 1440 pix.
= 14912.64 Å		10.36 Å/pix.
x 1023 pix.
= 10594.188 Å

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 10.356 Å
Density

Histogram

Histogram (log scale)
Minimum - Maximum-128.0 - 127.0
Average (Standard dev.)13.167028 (±6.840508)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-208208-80
Dimensions14401023160
Spacing10231440160
CellA: 10594.1875 Å / B: 14912.64 Å / C: 1656.96 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Influenza A virus (A/(H1N1))

EntireName: Influenza A virus (A/(H1N1))
Components
  • Virus: Influenza A virus (A/(H1N1))

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Supramolecule #1: Influenza A virus (A/(H1N1))

SupramoleculeName: Influenza A virus (A/(H1N1)) / type: virus / ID: 1 / Parent: 0 / Details: The virus was propagated in MDCK cells. / NCBI-ID: 3458231 / Sci species name: Influenza A virus (A/(H1N1)) / Virus type: VIRION / Virus isolate: OTHER / Virus enveloped: Yes / Virus empty: No

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Experimental details

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Structure determination

Methodcryo EM
Processingelectron tomography
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
GridModel: Quantifoil R2/1 / Material: COPPER / Mesh: 200
VitrificationCryogen name: ETHANE / Chamber humidity: 80 % / Chamber temperature: 90 K / Instrument: LEICA EM GP
SectioningOther: NO SECTIONING
Fiducial markerManufacturer: Aurion / Diameter: 10 nm

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 3.2 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 2.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionSoftware - Name: IMOD / Number images used: 41
CTF correctionSoftware - Name: IMOD / Type: PHASE FLIPPING ONLY

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