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- EMDB-55526: Staphylococcus aureus 70S initiation complex with a natural mRNA -

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Basic information

Entry
Database: EMDB / ID: EMD-55526
TitleStaphylococcus aureus 70S initiation complex with a natural mRNA
Map data
Sample
  • Complex: 70S ribosome initiation complex
    • Protein or peptide: x 47 types
    • RNA: x 5 types
KeywordsShine-Delgarno / anti Shine-Delgarno / translation initiation / Staphylococcus aureus. / RIBOSOME
Function / homology
Function and homology information


large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / ribosomal large subunit assembly / 5S rRNA binding / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding ...large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / ribosomal large subunit assembly / 5S rRNA binding / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / RNA binding / zinc ion binding / cytoplasm / cytosol
Similarity search - Function
Ribosomal protein L31 type B / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / : / Ribosomal protein S14, type Z / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily ...Ribosomal protein L31 type B / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / : / Ribosomal protein S14, type Z / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L16 signature 1. / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / : / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / : / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L28/L24 superfamily / : / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / : / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L6, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S4, bacterial-type / Ribosomal protein L5, bacterial-type / Ribosomal protein S5, bacterial-type / 30S ribosomal protein S17 / : / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein S14/S29 / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 / Ribosomal protein S9, bacterial/plastid / Ribosomal L28 family / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L33 superfamily / Ribosomal protein L16 / Ribosomal protein L28/L24 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / Ribosomal protein L30, bacterial-type / Ribosomal protein S15, bacterial-type / L28p-like / : / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein S12, bacterial-type / Ribosomal protein L20 / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal L32p protein family / Ribosomal protein L21
Similarity search - Domain/homology
Large ribosomal subunit protein bL25 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL2 ...Large ribosomal subunit protein bL25 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein bL31B / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL27 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL33B / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS15
Similarity search - Component
Biological speciesStaphylococcus aureus (bacteria) / Escherichia coli (E. coli)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.3 Å
AuthorsBahena Ceron R / Klaholz B / Marzi S
Funding support France, 1 items
OrganizationGrant numberCountry
Agence Nationale de la Recherche (ANR) France
CitationJournal: Nat Commun / Year: 2026
Title: Extended Shine-Dalgarno motifs govern translation initiation in Staphylococcus aureus.
Authors: Maximilian P Kohl / Roberto Bahena-Ceron / Béatrice Chane-Woon-Ming / Maria Kompatscher / Matthias D Erlacher / Charles Barchet / Ottilie von Loeffelholz / Pascale Romby / Bruno P Klaholz / Stefano Marzi /
Abstract: Regulation of translation initiation is central to bacterial adaptation, but species-specific mechanisms remain poorly understood. We present high-resolution mapping of translation start sites in ...Regulation of translation initiation is central to bacterial adaptation, but species-specific mechanisms remain poorly understood. We present high-resolution mapping of translation start sites in Staphylococcus aureus, revealing distinct features of initiation alongside numerous unannotated small ORFs. Our analysis, combined with cryo-EM of a native mRNA-ribosome complex, shows that S. aureus relies on extended, start codon proximal Shine-Dalgarno (SD) interactions, creating specificity against phylogenetically distant bacteria. Several natural S. aureus initiation sites are not correctly decoded by E. coli ribosomes. We identify new and conserved non-canonical start codons, whose regulatory initiation sites contain these characteristic extended SD sequence motifs. Finally, we characterize a novel example of uORF-mediated translational control in S. aureus, demonstrating that translation of a small leader peptide modulates expression of a key biofilm regulator. The described mechanism involves codon rarity, ribosome pausing, and arginine availability, linking nutrient sensing to biofilm formation in this major human pathogen.
History
DepositionOct 30, 2025-
Header (metadata) releaseFeb 25, 2026-
Map releaseFeb 25, 2026-
UpdateFeb 25, 2026-
Current statusFeb 25, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_55526.png

Downloads & links

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Map

FileDownload / File: emd_55526.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
0.73 Å/pix.
x 600 pix.
= 437.4 Å		0.73 Å/pix.
x 600 pix.
= 437.4 Å		0.73 Å/pix.
x 600 pix.
= 437.4 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.729 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.078
Minimum - Maximum-0.32402357 - 1.0559887
Average (Standard dev.)-0.000114880844 (±0.02676556)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions600600600
Spacing600600600
CellA=B=C: 437.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_55526_half_map_1.map
Projections & Slices
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Half map: #1

Fileemd_55526_half_map_2.map
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Sample components

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Entire : 70S ribosome initiation complex

EntireName: 70S ribosome initiation complex
Components
  • Complex: 70S ribosome initiation complex
    • Protein or peptide: Large ribosomal subunit protein bL33B
    • Protein or peptide: Large ribosomal subunit protein bL34
    • Protein or peptide: Large ribosomal subunit protein bL35
    • Protein or peptide: Large ribosomal subunit protein bL36
    • Protein or peptide: Large ribosomal subunit protein bL31B
    • RNA: 16S rRNA
    • Protein or peptide: Small ribosomal subunit protein uS2
    • Protein or peptide: Small ribosomal subunit protein uS3
    • Protein or peptide: Small ribosomal subunit protein uS4
    • Protein or peptide: Small ribosomal subunit protein uS5
    • Protein or peptide: Small ribosomal subunit protein bS6
    • Protein or peptide: Small ribosomal subunit protein uS7
    • Protein or peptide: Small ribosomal subunit protein uS8
    • Protein or peptide: Small ribosomal subunit protein uS9
    • Protein or peptide: Large ribosomal subunit protein uL13
    • Protein or peptide: Small ribosomal subunit protein uS11
    • Protein or peptide: Small ribosomal subunit protein uS12
    • Protein or peptide: Small ribosomal subunit protein uS13
    • Protein or peptide: Small ribosomal subunit protein uS14B
    • Protein or peptide: Small ribosomal subunit protein uS15
    • Protein or peptide: Small ribosomal subunit protein bS16
    • Protein or peptide: Small ribosomal subunit protein uS17
    • Protein or peptide: Small ribosomal subunit protein bS18
    • Protein or peptide: Small ribosomal subunit protein uS19
    • Protein or peptide: Small ribosomal subunit protein bS20
    • Protein or peptide: Large ribosomal subunit protein uL14
    • RNA: aur mRNA
    • RNA: Methionine tRNA
    • RNA: 23S rRNA
    • RNA: 5S rRNA
    • Protein or peptide: Large ribosomal subunit protein uL2
    • Protein or peptide: Large ribosomal subunit protein uL3
    • Protein or peptide: Large ribosomal subunit protein uL4
    • Protein or peptide: Large ribosomal subunit protein uL5
    • Protein or peptide: Large ribosomal subunit protein uL6
    • Protein or peptide: Small ribosomal subunit protein uS10
    • Protein or peptide: Large ribosomal subunit protein uL15
    • Protein or peptide: Large ribosomal subunit protein uL16
    • Protein or peptide: Large ribosomal subunit protein bL17
    • Protein or peptide: Large ribosomal subunit protein uL18
    • Protein or peptide: Large ribosomal subunit protein bL19
    • Protein or peptide: Large ribosomal subunit protein bL20
    • Protein or peptide: Large ribosomal subunit protein bL21
    • Protein or peptide: Large ribosomal subunit protein uL22
    • Protein or peptide: Large ribosomal subunit protein uL23
    • Protein or peptide: Large ribosomal subunit protein uL24
    • Protein or peptide: Large ribosomal subunit protein bL25
    • Protein or peptide: Large ribosomal subunit protein bL27
    • Protein or peptide: Large ribosomal subunit protein bL28
    • Protein or peptide: Large ribosomal subunit protein uL29
    • Protein or peptide: Large ribosomal subunit protein uL30
    • Protein or peptide: Large ribosomal subunit protein bL32

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Supramolecule #1: 70S ribosome initiation complex

SupramoleculeName: 70S ribosome initiation complex / type: complex / ID: 1 / Parent: 0
Macromolecule list: #1, #3, #5, #7, #9, #11, #13, #15, #17, #19, #21, #23, #25, #27, #29, #31, #33, #35, #37, #39, #41, #43, #45, #47, #49, #51, #2, #4, #6, #8, #10, #12, #14, #16, #18, #20, #22, ...Macromolecule list: #1, #3, #5, #7, #9, #11, #13, #15, #17, #19, #21, #23, #25, #27, #29, #31, #33, #35, #37, #39, #41, #43, #45, #47, #49, #51, #2, #4, #6, #8, #10, #12, #14, #16, #18, #20, #22, #24, #26, #28, #30, #32, #34, #36, #38, #40, #42, #44, #46, #48, #50, #52
Source (natural)Organism: Staphylococcus aureus (bacteria)

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Macromolecule #1: Large ribosomal subunit protein bL33B

MacromoleculeName: Large ribosomal subunit protein bL33B / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.885783 KDa
SequenceString:
MRVNVTLACT ECGDRNYITT KNKRNNPERV EMKKFCSREN KQTLHRETK

UniProtKB: Large ribosomal subunit protein bL33B

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Macromolecule #3: Large ribosomal subunit protein bL34

MacromoleculeName: Large ribosomal subunit protein bL34 / type: protein_or_peptide / ID: 3 / Details: 50S ribosomal protein L34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.454642 KDa
SequenceString:
MVKRTYQPNK RKHSKVHGFR KRMSTKNGRK VLARRRRKGR KVLSA

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #5: Large ribosomal subunit protein bL35

MacromoleculeName: Large ribosomal subunit protein bL35 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 7.722368 KDa
SequenceString:
MPKMKTHRGA AKRVKRTASG QLKRSRAFTS HLFANKSTKQ KRQLRKARLV SKSDMKRVKQ LLAYKK

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #7: Large ribosomal subunit protein bL36

MacromoleculeName: Large ribosomal subunit protein bL36 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 4.318422 KDa
SequenceString:
MKVRPSVKPI CEKCKVIKRK GKVMVICENP KHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #9: Large ribosomal subunit protein bL31B

MacromoleculeName: Large ribosomal subunit protein bL31B / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.580719 KDa
SequenceString:
MKQGIHPEYH QVIFLDTTTN FKFLSGSTKT SSEMMEWEDG KEYPVIRLDI SSDSHPFYTG RQKFAAADGV ERFNKKFGLK SNN

UniProtKB: Large ribosomal subunit protein bL31B

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Macromolecule #10: Large ribosomal subunit protein uL2

MacromoleculeName: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 30.217164 KDa
SequenceString: MAIKKYKPIT NGRRNMTSLD FAEITKTTPE KSLLKPLPKK AGRNNQGKLT VRHHGGGHKR QYRVIDFKRN KDGINAKVDS IQYDPNRSA NIALVVYADG EKRYIIAPKG LEVGQIVESG AEADIKVGNA LPLQNIPVGT VVHNIELKPG KGGQIARSAG A SAQVLGKE ...String:
MAIKKYKPIT NGRRNMTSLD FAEITKTTPE KSLLKPLPKK AGRNNQGKLT VRHHGGGHKR QYRVIDFKRN KDGINAKVDS IQYDPNRSA NIALVVYADG EKRYIIAPKG LEVGQIVESG AEADIKVGNA LPLQNIPVGT VVHNIELKPG KGGQIARSAG A SAQVLGKE GKYVLIRLRS GEVRMILSTC RATIGQVGNL QHELVNVGKA GRSRWKGIRP TVRGSVMNPN DHPHGGGEGR AP IGRPSPM SPWGKPTLGK KTRRGKKSSD KLIVRGRKKK

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #12: Large ribosomal subunit protein uL3

MacromoleculeName: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 23.760256 KDa
SequenceString: MTKGILGRKI GMTQVFGENG ELIPVTVVEA KENVVLQKKT VEVDGYNAIQ VGFEDKKAYK KDAKSNKYAN KPAEGHAKKA DAAPKRFIR EFRNVDVDAY EVGQEVSVDT FVAGDVIDVT GVSKGKGFQG AIKRHGQSRG PMSHGSHFHR APGSVGMASD A SRVFKGQK ...String:
MTKGILGRKI GMTQVFGENG ELIPVTVVEA KENVVLQKKT VEVDGYNAIQ VGFEDKKAYK KDAKSNKYAN KPAEGHAKKA DAAPKRFIR EFRNVDVDAY EVGQEVSVDT FVAGDVIDVT GVSKGKGFQG AIKRHGQSRG PMSHGSHFHR APGSVGMASD A SRVFKGQK MPGRMGGNTV TVQNLEVVQV DTENKVILVK GNVPGPKKGL VEIRTSIKKG NK

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #13: Small ribosomal subunit protein uS2

MacromoleculeName: Small ribosomal subunit protein uS2 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 29.136369 KDa
SequenceString: MAVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKKV DEAYNFLKQV SEDGGQVLFV GTKKQAQESV KSEAERAGQ FYINQRWLGG LLTNYKTISK RIKRISEIEK MEEDGLFEVL PKKEVVELKK EYDRLIKFLG GIRDMKSMPQ A LFVVDPRK ...String:
MAVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKKV DEAYNFLKQV SEDGGQVLFV GTKKQAQESV KSEAERAGQ FYINQRWLGG LLTNYKTISK RIKRISEIEK MEEDGLFEVL PKKEVVELKK EYDRLIKFLG GIRDMKSMPQ A LFVVDPRK ERNAIAEARK LNIPIVGIVD TNCDPDEIDY VIPANDDIAR AVKLLTAKMA DAILEGQQGV SNEEVAAEQN ID LDEKEKS EETEATEE

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #14: Large ribosomal subunit protein uL4

MacromoleculeName: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 22.495697 KDa
SequenceString: MANYDVLKLD GTKSGSIELS DAVFGIEPNN SVLFEAINLQ RASLRQGTHA VKNRSAVSGG GRKPWKQKGT GRARQGTIRA PQWRGGGIV FGPTPRSYAY KMPKKMRRLA LRSALSFKAQ ENGLTVVDAF NFEAPKTKEF KNVLSTLEQP KKVLVVTENE D VNVELSAR ...String:
MANYDVLKLD GTKSGSIELS DAVFGIEPNN SVLFEAINLQ RASLRQGTHA VKNRSAVSGG GRKPWKQKGT GRARQGTIRA PQWRGGGIV FGPTPRSYAY KMPKKMRRLA LRSALSFKAQ ENGLTVVDAF NFEAPKTKEF KNVLSTLEQP KKVLVVTENE D VNVELSAR NIPGVQVTTA QGLNVLDITN ADSLVITEAA AKKVEEVLG

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #15: Small ribosomal subunit protein uS3

MacromoleculeName: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 24.143867 KDa
SequenceString: MGQKINPIGL RVGIIRDWEA KWYAEKDFAS LLHEDLKIRK FIDNELKEAS VSHVEIERAA NRINIAIHTG KPGMVIGKGG SEIEKLRNK LNALTDKKVH INVIEIKKVD LDARLVAENI ARQLENRASF RRVQKQAITR AMKLGAKGIK TQVSGRLGGA D IARAEQYS ...String:
MGQKINPIGL RVGIIRDWEA KWYAEKDFAS LLHEDLKIRK FIDNELKEAS VSHVEIERAA NRINIAIHTG KPGMVIGKGG SEIEKLRNK LNALTDKKVH INVIEIKKVD LDARLVAENI ARQLENRASF RRVQKQAITR AMKLGAKGIK TQVSGRLGGA D IARAEQYS EGTVPLHTLR ADIDYAHAEA DTTYGKLGVK VWIYRGEVLP TKNTSGGGK

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #16: Large ribosomal subunit protein uL5

MacromoleculeName: Large ribosomal subunit protein uL5 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 20.296637 KDa
SequenceString:
MNRLKEKFNT EVTENLMKKF NYSSVMEVPK IDKIVVNMGV GDAVQNSKVL DNAVEELELI TGQKPLVTKA KKSIATFRLR EGMPIGAKV TLRGERMYEF LDKLISVSLP RVRDFQGVSK KAFDGRGNYT LGVKEQLIFP EIDYDKVSKV RGMDIVIVTT A NTDEEARE LLANFGMPFR K

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #17: Small ribosomal subunit protein uS4

MacromoleculeName: Small ribosomal subunit protein uS4 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 23.023363 KDa
SequenceString: MARFRGSNWK KSRRLGISLS GTGKDVEKRP YAPGQHGPNQ RKKLSEYGLQ LREKQKLRYL YGMTERQFRN TFDIAGKKFG VHGENFMIL LASRLDAVVY SLGLARTRRQ ARQLVNHGHI LVDGKRVDIP SYSVKPGQTI SVREKSQKLN IIVESVEINN F VPEYLNFD ...String:
MARFRGSNWK KSRRLGISLS GTGKDVEKRP YAPGQHGPNQ RKKLSEYGLQ LREKQKLRYL YGMTERQFRN TFDIAGKKFG VHGENFMIL LASRLDAVVY SLGLARTRRQ ARQLVNHGHI LVDGKRVDIP SYSVKPGQTI SVREKSQKLN IIVESVEINN F VPEYLNFD ADSLTGTFVR LPERSELPAE INEQLIVEYY SR

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #18: Large ribosomal subunit protein uL6

MacromoleculeName: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 19.632348 KDa
SequenceString:
MSRVGKKIID IPSDVTVTFD GNHVTVKGPK GELSRTLNER MTFKQEENTI EVVRPSDSKE DRTNHGTTRA LLNNMVQGVS QGYVKVLEL VGVGYRAQMQ GKDLILNVGY SHPVEIKAEE NITFSVEKNT VVKVEGISKE QVGALASNIR SVRPPEPYKG K GIRYQGEY VRRKEGKT

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #19: Small ribosomal subunit protein uS5

MacromoleculeName: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 17.770512 KDa
SequenceString:
MARREEETKE FEERVVTINR VAKVVKGGRR FRFTALVVVG DKNGRVGFGT GKAQEVPEAI KKAVEAAKKD LVVVPRVEGT TPHTITGRY GSGSVFMKPA APGTGVIAGG PVRAVLELAG ITDILSKSLG SNTPINMVRA TIDGLQNLKN AEDVAKLRGK T VEELYN

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #20: Small ribosomal subunit protein uS10

MacromoleculeName: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.598503 KDa
SequenceString:
MAKQKIRIRL KAYDHRVIDQ SAEKIVETAK RSGADVSGPI PLPTEKSVYT IIRAVHKYKD SREQFEQRTH KRLIDIVNPT PKTVDALMG LNLPSGVDIE IKL

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #21: Small ribosomal subunit protein bS6

MacromoleculeName: Small ribosomal subunit protein bS6 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.613146 KDa
SequenceString:
MRTYEVMYIV RPNIEEDAKK ALVERFNGIL ATEGAEVLEA KDWGKRRLAY EINDFKDGFY NIVRVKSDNN KATDEFQRLA KISDDIIRY MVIREDEDK

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #22: Large ribosomal subunit protein uL15

MacromoleculeName: Large ribosomal subunit protein uL15 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 15.62889 KDa
SequenceString:
MKLHELKPAE GSRKERNRVG RGVATGNGKT SGRGHKGQKA RSGGGVRPGF EGGQLPLFRR LPKRGFTNIN RKEYAIVNLD QLNKFEDGT EVTPALLVES GVVKNEKSGI KILGNGSLDK KLTVKAHKFS ASAAEAIDAK GGAHEVI

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #23: Small ribosomal subunit protein uS7

MacromoleculeName: Small ribosomal subunit protein uS7 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 17.727422 KDa
SequenceString:
MPRKGSVPKR DVLPDPIHNS KLVTKLINKI MLDGKRGTAQ RILYSAFDLV EQRSGRDALE VFEEAINNIM PVLEVKARRG GSNYQVPVE VRPERRTTLG LRWLVNYARL RGEKTMEDRL ANEILDAANN TGGAVKKRED THKMAEANKA FAHYRW

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #24: Large ribosomal subunit protein uL16

MacromoleculeName: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 16.274049 KDa
SequenceString:
MLLPKRVKYR RQHRPKTTGR SKGGNYVTFG EFGLQATTTS WITSRQIESA RIAMTRYMKR GGKVWIKIFP HTPYTKKPLE VRMGAGKGA VEGWIAVVKP GRILFEVAGV SEEVAREALR LASHKLPVKT KFVKREELGG ETNES

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #25: Small ribosomal subunit protein uS8

MacromoleculeName: Small ribosomal subunit protein uS8 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 14.854315 KDa
SequenceString:
MTMTDPIADM LTRVRNANMV RHEKLELPAS NIKKEIAEIL KSEGFIKNVE YVEDDKQGVL RLFLKYGQND ERVITGLKRI SKPGLRVYA KASEMPKVLN GLGIALVSTS EGVITDKEAR KRNVGGEIIA YVW

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #26: Large ribosomal subunit protein bL17

MacromoleculeName: Large ribosomal subunit protein bL17 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.771773 KDa
SequenceString:
MGYRKLGRTS DQRKAMLRDL ATSLIISERI ETTEARAKEV RSVVEKLITL GKKGDLASRR NAAKTLRNVE ILNEDETTQT ALQKLFGEI AERYTERQGG YTRILKQGPR RGDGAESVII ELV

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #27: Small ribosomal subunit protein uS9

MacromoleculeName: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 14.727808 KDa
SequenceString:
MTLAQVEYRG TGRRKNSVAR VRLVPGEGNI TVNNRDVREY LPFESLILDL NQPFDVTETG NYDVLVNVHG GGFTGQAQAI RHGIARALL EADPEYRGSL KRAGLLTRDP RMKERKKPGL KAARRSPQFS KR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #28: Large ribosomal subunit protein uL18

MacromoleculeName: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.080083 KDa
SequenceString:
MISKIDKNKV RLKRHARVRT NLSGTAEKPR LNVYRSNKHI YAQIIDDNKG VTLAQASSKD SIAATTATKV ELATKVGEAI AKKAADKGI KEIVFDRGGY LYHGRVKALA EAARESGLEF

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #29: Large ribosomal subunit protein uL13

MacromoleculeName: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 16.359427 KDa
SequenceString:
MRQTFMANES NIERKWYVID AEGQTLGRLS SEVASILRGK NKVTYTPHVD TGDYVIVINA SKIEFTGNKE TDKVYYRHSN HPGGIKSIT AGELRRTNPE RLIENSIKGM LPSTRLGEKQ GKKLFVYGGA EHPHAAQQPE NYELRG

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #30: Large ribosomal subunit protein bL19

MacromoleculeName: Large ribosomal subunit protein bL19 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.392771 KDa
SequenceString:
MTNHKLIEAV TKSQLRTDLP SFRPGDTLRV HVRIIEGTRE RIQVFEGVVI KRRGGGVSET FTVRKISSGV GVERTFPLHT PKIEKIEVK RRGKVRRAKL YYLRSLRGKA ARIQEIR

UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #31: Small ribosomal subunit protein uS11

MacromoleculeName: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.879964 KDa
SequenceString:
MARKQVSRKR RVKKNIENGV AHIRSTFNNT IVTITDEFGN ALSWSSAGAL GFKGSKKSTP FAAQMASETA SKSAMEHGLK TVEVTVKGP GPGRESAIRA LQSAGLEVTA IRDVTPVPHN GCRPPKKRRV

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #32: Large ribosomal subunit protein bL20

MacromoleculeName: Large ribosomal subunit protein bL20 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.720295 KDa
SequenceString:
MPRVKGGTVT RARRKKTIKL AKGYFGSKHT LYKVAKQQVM KSGQYAFRDR RQRKRDFRKL WITRINAAAR QHEMSYSRLM NGLKKAGID INRKMLSEIA ISDEKAFAQL VTKAKDALK

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #33: Small ribosomal subunit protein uS12

MacromoleculeName: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 15.376897 KDa
SequenceString:
MPTINQLVRK PRQSKIKKSD SPALNKGFNS KKKKFTDLNS PQKRGVCTRV GTMTPRKPNS ALRKYARVRL SNNIEINAYI PGIGHNLQE HSVVLVRGGR VRDLPGVRYH IVRGALDTSG VDGRRQGRSL YGTKKPKN

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #34: Large ribosomal subunit protein bL21

MacromoleculeName: Large ribosomal subunit protein bL21 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.354081 KDa
SequenceString:
MFAIIETGGK QIKVEEGQEI FVEKLDVNEG DTFTFDKVLF VGGDSVKVGA PTVEGATVTA TVNKQGRGKK ITVFTYKRRK NSKRKKGHR QPYTKLTIDK INA

UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #35: Small ribosomal subunit protein uS13

MacromoleculeName: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.747919 KDa
SequenceString:
MARIAGVDIP REKRVVISLT YIYGIGTSTA QKILEEANVS ADTRVKDLTD DELGRIREVV DGYKVEGDLR RETNLNIKRL MEISSYRGI RHRRGLPVRG QKTKNNARTR KGPVKTVANK KK

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #36: Large ribosomal subunit protein uL22

MacromoleculeName: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 12.857922 KDa
SequenceString:
MEAKAVARTI RIAPRKVRLV LDLIRGKNAA EAIAILKLTN KASSPVIEKV LMSALANAEH NYDMNTDELV VKEAYANEGP TLKRFRPRA QGRASAINKR TSHITIVVSD GKEEAKEA

UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #37: Small ribosomal subunit protein uS14B

MacromoleculeName: Small ribosomal subunit protein uS14B / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 7.317769 KDa
SequenceString:
MAKTSMVAKQ QKKQKYAVRE YTRCERCGRP HSVYRKFKLC RICFRELAYK GQIPGVRKAS W

UniProtKB: Small ribosomal subunit protein uS14B

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Macromolecule #38: Large ribosomal subunit protein uL23

MacromoleculeName: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.625398 KDa
SequenceString:
MEARDILKRP VITEKSSEAM AEDKYTFDVD TRVNKTQVKM AVEEIFNVKV ASVNIMNYKP KKKRMGRYQG YTNKRRKAIV TLKEGSIDL FN

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #39: Small ribosomal subunit protein uS15

MacromoleculeName: Small ribosomal subunit protein uS15 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.63433 KDa
SequenceString:
MAISQERKNE IIKEYRVHET DTGSPEVQIA VLTAEINAVN EHLRTHKKDH HSRRGLLKMV GRRRHLLNYL RSKDIQRYRE LIKSLGIRR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #40: Large ribosomal subunit protein uL24

MacromoleculeName: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.839577 KDa
SequenceString:
IKKGDNVKVI AGKDKGKEGK VIATLPKKDR VVVEGVNIMK KHQKNAQGIE TEAAIHVSNV QLLDPKTNEP TRVGYKFVDG KKVRIAKKS G

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #41: Small ribosomal subunit protein bS16

MacromoleculeName: Small ribosomal subunit protein bS16 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.253886 KDa
SequenceString:
MAVKIRLTRL GSKRNPFYRI VVADARSPRD GRIIEQIGTY NPTSANAPEI KVDEALALKW LNDGAKPTDT VHNILSKEGI MKKFDEQKK AK

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #42: Large ribosomal subunit protein bL25

MacromoleculeName: Large ribosomal subunit protein bL25 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 23.810609 KDa
SequenceString: MASLKSIIRQ GKQTRSDLKQ LRKSGKVPAV VYGYGTKNVS VKVDEVEFIK VIREVGRNGV IELGVGSKTI KVMVADYQFD PLKNQITHI DFLAINMSEE RTVEVPVQLV GEAVGAKEGG VVEQPLFNLE VTATPDNIPE AIEVDITELN INDSLTVADV K VTGDFKIE ...String:
MASLKSIIRQ GKQTRSDLKQ LRKSGKVPAV VYGYGTKNVS VKVDEVEFIK VIREVGRNGV IELGVGSKTI KVMVADYQFD PLKNQITHI DFLAINMSEE RTVEVPVQLV GEAVGAKEGG VVEQPLFNLE VTATPDNIPE AIEVDITELN INDSLTVADV K VTGDFKIE NDSAESVVTV VAPTEEPTEE EIEAMEGEQQ TEEPEVVGES KEDEEKTEE

UniProtKB: Large ribosomal subunit protein bL25

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Macromolecule #43: Small ribosomal subunit protein uS17

MacromoleculeName: Small ribosomal subunit protein uS17 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.196888 KDa
SequenceString:
MSERNDRKVY VGKVVSDKMD KTITVLVETY KTHKLYGKRV KYSKKYKTHD ENNSAKLGDI VKIQETRPLS ATKRFRLVEI VEESVII

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #44: Large ribosomal subunit protein bL27

MacromoleculeName: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.334798 KDa
SequenceString:
MLKLNLQFFA SKKGVSSTKN GRDSESKRLG AKRADGQFVT GGSILYRQRG TKIYPGENVG RGGDDTLFAK IDGVVKFERK GRDKKQVSV YAVAE

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #45: Small ribosomal subunit protein bS18

MacromoleculeName: Small ribosomal subunit protein bS18 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.332018 KDa
SequenceString:
MAGGPRRGGR RRKKVCYFTA NGITHIDYKD TELLKRFISE RGKILPRRVT GTSAKYQRML TTAIKRSRHM ALLPYVKEEQ

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #46: Large ribosomal subunit protein bL28

MacromoleculeName: Large ribosomal subunit protein bL28 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.995289 KDa
SequenceString:
MGKQCFVTGR KASTGNRRSH ALNSTKRRWN ANLQKVRILV DGKPKKVWVS ARALKSGKVT RV

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #47: Small ribosomal subunit protein uS19

MacromoleculeName: Small ribosomal subunit protein uS19 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.639309 KDa
SequenceString:
MARSIKKGPF VDEHLMKKVE AQEGSEKKQV IKTWSRRSTI FPNFIGHTFA VYDGRKHVPV YVTEDMVGHK LGEFAPTRTF KGHVADDKK TRR

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #48: Large ribosomal subunit protein uL29

MacromoleculeName: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 8.105266 KDa
SequenceString:
MKAKEIRDLT TSEIEEQIKS SKEELFNLRF QLATGQLEET ARIRTVRKTI ARLKTVARER EIEQSKANQ

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #49: Small ribosomal subunit protein bS20

MacromoleculeName: Small ribosomal subunit protein bS20 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.012446 KDa
SequenceString:
MANIKSAIKR VKTTEKAEAR NISQKSAMRT AVKNAKTAVS NNADNKNELV SLAVKLVDKA AQSNLIHSNK ADRIKSQLMT ASK

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #50: Large ribosomal subunit protein uL30

MacromoleculeName: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.565683 KDa
SequenceString:
MAKLQITLTR SVIGRPETQR KTVEALGLKK TNSSVVVEDN PAIRGQINKV KHLVTVEEK

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #51: Large ribosomal subunit protein uL14

MacromoleculeName: Large ribosomal subunit protein uL14 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.157342 KDa
SequenceString:
MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKNATPGG VVKKGDVVKA VIVRTKSGVR RNDGSYIKFD ENACVIIRD DKGPRGTRIF GPVARELREG NFMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #52: Large ribosomal subunit protein bL32

MacromoleculeName: Large ribosomal subunit protein bL32 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.500609 KDa
SequenceString:
MAVPKRRTSK TRKNKRRTHF KISVPGMTEC PNCGEYKLSH RVCKNCGSYN GEEVAAK

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #2: aur mRNA

MacromoleculeName: aur mRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.594422 KDa
SequenceString:
AGGAGGAAUG AAAUGUG

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Macromolecule #4: Methionine tRNA

MacromoleculeName: Methionine tRNA / type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 24.497602 KDa
SequenceString:
GCGGGGUGGA GCAGCCUGGU AGCUCGUCGG GCUCAUAACC CGAAGGUCGU CGGUUCAAAU CCGGCCCCCG CAACCA

GENBANK: GENBANK: AP023205.1

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Macromolecule #6: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 6 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 944.65025 KDa
SequenceString: UUAAGUUAUU AAGGGCGCAC GGUGGAUGCC UUGGCACUAG AAGCCGAUGA AGGACGUUAC UAACGACGAU AUGCUUUGGG GAGCUGUAA GUAAGCUUUG AUCCAGAGAU UUCCGAAUGG GGAAACCCAG CAUGAGUUAU GUCAUGUUAU CGAUAUGUGA A UACAUAGC ...String:
UUAAGUUAUU AAGGGCGCAC GGUGGAUGCC UUGGCACUAG AAGCCGAUGA AGGACGUUAC UAACGACGAU AUGCUUUGGG GAGCUGUAA GUAAGCUUUG AUCCAGAGAU UUCCGAAUGG GGAAACCCAG CAUGAGUUAU GUCAUGUUAU CGAUAUGUGA A UACAUAGC AUAUCAGAAG GCACACCCGG AGAACUGAAA CAUCUUAGUA CCCGGAGGAA GAGAAAGAAA AUUCGAUUCC CU UAGUAGC GGCGAGCGAA AUGGGAAGAG CCCAAACCAA CAAGCUUGCU UGUUGGGGUU GUAGGACACU CUAUACGGAG UUA CAAAGG ACGACAUUAG ACGAAUCAUC UGGAAAGAUG AAUCAAAGAA GGUAAUAAUC CUGUAGUCGA AAAUGUUGUC UCUC UUGAG UGCAUCCUGA GUACGACGGA GCACGUGAAA UUCCGUCGGA AUCUGGGAGG ACCAUCUCCU AAGGCUAAAU ACUCU CUAG UGACCGAUAG UGAACCAGUA CCGUGAGGGA AAGGUGAAAA GCACCCCGGA AGGGGAGUGA AAUAGAACCU GAAACC GUG UGCUUACAAG UAGUCAGAGC CCGUUAAUGG GUGAUGGCGU GCCUUUUGUA GAAUGAACCG GCGAGUUACG AUUUGAU GC AAGGUUAAGC AGUAAAUGUG GAGCCGUAGC GAAAGCGAGU CUGAAUAGGG CGUUUAGUAU UUGGUCGUAG ACCCGAAA C CAGGUGAUCU ACCCUUGGUC AGGUUGAAGU UCAGGUAACA CUGAAUGGAG GACCGAACCG ACUUACGUUG AAAAGUGAG CGGAUGAACU GAGGGUAGCG GAGAAAUUCC AAUCGAACCU GGAGAUAGCU GGUUCUCUCC GAAAUAGCUU UAGGGCUAGC CUCAAGUGA UGAUUAUUGG AGGUAGAGCA CUGUUUGGAC GAGGGGCCCC UCUCCGGGUU ACCGAAUUCA GACAAACUCC G AAUGCCAA UUAAUUUAAC UUGGGAGUCA GAACAUGGGU GAUAAGGUCC GUGUUCGAAA GGGAAACAGC CCAGACCACC AG CUAAGGU CCCAAAAUAU AUGUUAAGUG GAAAAGGAUG UGGCGUUGCC CAGACAACUA GGAUGUUGGC UUAGAAGCAG CCA UCAUUU AAAGAGUGCG UAAUAGCUCA CUAGUCGAGU GACACUGCGC CGAAAAUGUA CCGGGGCUAA ACAUAUUACC GAAG CUGUG GAUUGUCCUU UGGACAAUGG UAGGAGAGCG UUCUAAGGGC GUUGAAGCAU GAUCGUAAGG ACAUGUGGAG CGCUU AGAA GUGAGAAUGC CGGUGUGAGU CGCGAAAGAC GGGUGAGAAU CCCGUCCACC GAUUGACUAA GGUUUCCAGA GGAAGG CUC GUCCGCUCUG GGUUAGUCGG GUCCUAAGCU GAGGCCGACA GGCGUAGGCG AUGGAUAACA GGUUGAUAUU CCUGUAC CA CCUAUAAUCG UUUUAAUCGA UGGGGGGACG CAGUAGGAUA GGCGAAGCGU GCGAUUGGAU UGCACGUCUA AGUAGUAA G GCUGAGUAUU AGGCAAAUCC GGUACUCGUU AAGGCUGAGC UGUGAUGGGG AGAAGACAUU GUCUUCGAGU CGUUGAUUU CACACUGCCG AGAAAAGCCU CUAGAUAGAA AAUAGGUGCC CGUACCGCAA ACCGACACAG GUAGUCAAGA UGAGAAUUCU AAGGUGAGC GAGCGAACUC UCGUUAAGGA ACUCGGCAAA AUGACCCCGU AACUUCGGGA GAAGGGGUGC UCUUUAGGGU U AACGCCCA GAAGAGCCGC AGUGAAUAGG CCCAAGCGAC UGUUUAUCAA AAACACAGGU CUCUGCUAAA CCGUAAGGUG AU GUAUAGG GGCUGACGCC UGCCCGGUGC UGGAAGGUUA AGAGGAGUGG UUAGCUUCUG CGAAGCUACG AAUCGAAGCC CCA GUAAAC GGCGGCCGUA ACUAUAACGG UCCUAAGGUA GCGAAAUUCC UUGUCGGGUA AGUUCCGACC CGCACGAAAG GCGU AACGA UUUGGGCACU GUCUCAACGA GAGACUCGGU GAAAUCAUAG UACCUGUGAA GAUGCAGGUU ACCCGCGACA GGACG GAAA GACCCCGUGG AGCUUUACUG UAGCCUGAUA UUGAAAUUCG GCACAGCUUG UACAGGAUAG GUAGGAGCCU UUGAAA CGU GAGCGCUAGC UUACGUGGAG GCGCUGGUGG GAUACUACCC UAGCUGUGUU GGCUUUCUAA CCCGCACCAC UUAUCGU GG UGGGAGACAG UGUCAGGCGG GCAGUUUGAC UGGGGCGGUC GCCUCCUAAA AGGUAACGGA GGCGCUCAAA GGUUCCCU C AGAAUGGUUG GAAAUCAUUC AUAGAGUGUA AAGGCAUAAG GGAGCUUGAC UGCGAGACCU ACAAGUCGAG CAGGGUCGA AAGACGGACU UAGUGAUCCG GUGGUUCCGC AUGGAAGGGC CAUCGCUCAA CGGAUAAAAG CUACCCCGGG GAUAACAGGC UUAUCUCCC CCAAGAGUUC ACAUCGACGG GGAGGUUUGG CACCUCGAUG UCGGCUCAUC GCAUCCUGGG GCUGUAGUCG G UCCCAAGG GUUGGGCUGU UCGCCCAUUA AAGCGGUACG CGAGCUGGGU UCAGAACGUC GUGAGACAGU UCGGUCCCUA UC CGUCGUG GGCGUAGGAA AUUUGAGAGG AGCUGUCCUU AGUACGAGAG GACCGGGAUG GACAUACCUC UGGUGUACCA GUU GUCGUG CCAACGGCAU AGCUGGGUAG CUAUGUGUGG ACGGGAUAAG UGCUGAAAGC AUCUAAGCAU GAAGCCCCCC UCAA GAUGA GAUUUCCCAA CUUCGGUUAU AAGAUCCCUC AAAGAUGAUG AGGUUAAUAG GUUCGAGGUG GAAGCAUGGU GACAU GUGG AGCUGACGAA UACUAAUCGA UCGAAGACUU AAU

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Macromolecule #8: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 8 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 36.97593 KDa
SequenceString:
UCUGGUGACU AUAGCAAGGA GGUCACACCU GUUCCCAUGC CGAACACAGA AGUUAAGCUC CUUAGCGUCG AUGGUAGUCG AACUUACGU UCCGUUAGAG UAGAACGUUG CCAGGC

GENBANK: GENBANK: CP077870.1

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Macromolecule #11: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 11 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 500.810594 KDa
SequenceString: UGGAGAGUUU GAUCCUGGCU CAGGAUGAAC GCUGGCGGCG UGCCUAAUAC AUGCAAGUCG AGCGAACGGA CGAGAAGCUU GCUUCUCUG AUGUUAGCGG CGGACGGGUG AGUAACACGU GGAUAACCUA CCUAUAAGAC UGGGAUAACU UCGGGAAACC G GAGCUAAU ...String:
UGGAGAGUUU GAUCCUGGCU CAGGAUGAAC GCUGGCGGCG UGCCUAAUAC AUGCAAGUCG AGCGAACGGA CGAGAAGCUU GCUUCUCUG AUGUUAGCGG CGGACGGGUG AGUAACACGU GGAUAACCUA CCUAUAAGAC UGGGAUAACU UCGGGAAACC G GAGCUAAU ACCGGAUAAU AUUUUGAACC GCAUGGUUCA AAAGUGAAAG ACGGUCUUGC UGUCACUUAU AGAUGGAUCC GC GCUGCAU UAGCUAGUUG GUAAGGUAAC GGCUUACCAA GGCAACGAUG CAUAGCCGAC CUGAGAGGGU GAUCGGCCAC ACU GGAACU GAGACACGGU CCAGACUCCU ACGGGAGGCA GCAGUAGGGA AUCUUCCGCA AUGGGCGAAA GCCUGACGGA GCAA CGCCG CGUGAGUGAU GAAGGUCUUC GGAUCGUAAA ACUCUGUUAU UAGGGAAGAA CAUAUGUGUA AGUAACUGUG CACAU CUUG ACGGUACCUA AUCAGAAAGC CACGGCUAAC UACGUGCCAG CAGCCGCGGU AAUACGUAGG UGGCAAGCGU UAUCCG GAA UUAUUGGGCG UAAAGCGCGC GUAGGCGGUU UUUUAAGUCU GAUGUGAAAG CCCACGGCUC AACCGUGGAG GGUCAUU GG AAACUGGAAA ACUUGAGUGC AGAAGAGGAA AGUGGAAUUC CAUGUGUAGC GGUGAAAUGC GCAGAGAUAU GGAGGAAC A CCAGUGGCGA AGGCGACUUU CUGGUCUGUA ACUGACGCUG AUGUGCGAAA GCGUGGGGAU CAAACAGGAU UAGAUACCC UGGUAGUCCA CGCCGUAAAC GAUGAGUGCU AAGUGUUAGG GGGUUUCCGC CCCUUAGUGC UGCAGCUAAC GCAUUAAGCA CUCCGCCUG GGGAGUACGA CCGCAAGGUU GAAACUCAAA GGAAUUGACG GGGACCCGCA CAAGCGGUGG AGCAUGUGGU U UAAUUCGA AGCAACGCGA AGAACCUUAC CAAAUCUUGA CAUCCUUUGA CAACUCUAGA GAUAGAGCCU UCCCCUUCGG GG GACAAAG UGACAGGUGG UGCAUGGUUG UCGUCAGCUC GUGUCGUGAG AUGUUGGGUU AAGUCCCGCA ACGAGCGCAA CCC UUAAGC UUAGUUGCCA UCAUUAAGUU GGGCACUCUA AGUUGACUGC CGGUGACAAA CCGGAGGAAG GUGGGGAUGA CGUC AAAUC AUCAUGCCCC UUAUGAUUUG GGCUACACAC GUGCUACAAU GGACAAUACA AAGGGCAGCG AAACCGCGAG GUCAA GCAA AUCCCAUAAA GUUGUUCUCA GUUCGGAUUG UAGUCUGCAA CUCGACUACA UGAAGCUGGA AUCGCUAGUA AUCGUA GAU CAGCAUGCUA CGGUGAAUAC GUUCCCGGGU CUUGUACACA CCGCCCGUCA CACCACGAGA GUUUGUAACA CCCGAAG CC GGUGGAGUAA CCUUUUAGGA GCUAGCCGUC GAAGGUGGGA CAAAUGAUUG GGGUGAAGUC GUAACAAGGU AGCCGUAU C GGAAGGUGCG GCUGGAUCAC CUCCUUU

GENBANK: GENBANK: CP030455.1

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 283 K

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 30.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.8000000000000003 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING ONLY
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6yef

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 316031
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.7.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD

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