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Yorodumi- EMDB-55225: CryoEM structure of Candida auris 80S ribosome in complex with Cy... -
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Basic information
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| Title | CryoEM structure of Candida auris 80S ribosome in complex with Cycloheximide and Geneticin G418 | |||||||||
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Keywords | Ribosome / Candida auris / Cycloheximide / Geneticin G418 / cryoEM | |||||||||
| Function / homology | Function and homology informationADP transmembrane transporter activity / negative regulation of cell integrity MAPK cascade / positive regulation of conjugation with cellular fusion / yeast-form cell wall / GCN2-mediated signaling / regulation of cytoplasmic translation / negative regulation of p38MAPK cascade / ribosome hibernation / preribosome / regulation of amino acid metabolic process ...ADP transmembrane transporter activity / negative regulation of cell integrity MAPK cascade / positive regulation of conjugation with cellular fusion / yeast-form cell wall / GCN2-mediated signaling / regulation of cytoplasmic translation / negative regulation of p38MAPK cascade / ribosome hibernation / preribosome / regulation of amino acid metabolic process / negative regulation of glucose mediated signaling pathway / pre-mRNA 5'-splice site binding / GDP-dissociation inhibitor activity / nonfunctional rRNA decay / preribosome, small subunit precursor / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / mRNA destabilization / preribosome, large subunit precursor / signaling receptor activator activity / negative regulation of mRNA splicing, via spliceosome / negative regulation of translational frameshifting / translational elongation / G-protein alpha-subunit binding / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal subunit export from nucleus / 90S preribosome / translational termination / protein-RNA complex assembly / ribosome-associated ubiquitin-dependent protein catabolic process / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / protein-membrane adaptor activity / ribosomal small subunit export from nucleus / DNA-(apurinic or apyrimidinic site) endonuclease activity / positive regulation of autophagy / rescue of stalled cytosolic ribosome / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / rRNA processing / cytosolic ribosome / large ribosomal subunit / ribosomal small subunit assembly / ribosome binding / ribosomal small subunit biogenesis / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / small ribosomal subunit rRNA binding / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / ribosome / translation / structural constituent of ribosome / G protein-coupled receptor signaling pathway / ribonucleoprotein complex / mRNA binding / nucleolus / RNA binding / zinc ion binding / membrane / nucleus / cytosol / cytoplasm Similarity search - Function | |||||||||
| Biological species | Candidozyma auris (fungus) | |||||||||
| Method | single particle reconstruction / cryo EM / Resolution: 1.99 Å | |||||||||
Authors | Atamas A / Stetsenko A / Incarnato D / Macia Valero A / Rogachev A / Billerbeck S / Guskov A | |||||||||
| Funding support | 1 items
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Citation | Journal: Structure / Year: 2026Title: Unveiling the Molecular Architecture of Candida auris Ribosome Authors: Atamas A / Stetsenko A / Incarnato D / Macia Valero A / Rogachev A / Billerbeck S / Guskov A | |||||||||
| History |
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Structure visualization
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Downloads & links
-EMDB archive
| Map data | emd_55225.map.gz | 291 MB | EMDB map data format | |
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| Header (meta data) | emd-55225-v30.xml emd-55225.xml | 95.1 KB 95.1 KB | Display Display | EMDB header |
| FSC (resolution estimation) | emd_55225_fsc_1.xml emd_55225_fsc_2.xml emd_55225_fsc_3.xml | 14.8 KB 14.8 KB 14.7 KB | Display Display Display | FSC data file |
| Images | emd_55225.png | 106.9 KB | ||
| Filedesc metadata | emd-55225.cif.gz | 18.8 KB | ||
| Archive directory | https://data.pdbj.org/pub/emdb/structures/EMD-55225 ftp://data.pdbj.org/pub/emdb/structures/EMD-55225 | HTTPS FTP |
-Related structure data
| Related structure data | ![]() 9sumMC ![]() 9spjC ![]() 9t7tC M: atomic model generated by this map C: citing same article ( |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
| EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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| Related items in Molecule of the Month |
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Map
| File | Download / File: emd_55225.map.gz / Format: CCP4 / Size: 347.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
| Voxel size | X=Y=Z: 0.836 Å | ||||||||||||||||||||||||||||||||||||
| Density |
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| Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
| Details | EMDB XML:
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-Supplemental data
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Sample components
+Entire : Candida auris 80S ribosome
+Supramolecule #1: Candida auris 80S ribosome
+Macromolecule #1: P-site tRNA
+Macromolecule #2: RNA (5'-R(P*CP*GP*A)-3')
+Macromolecule #3: 25S rRNA
+Macromolecule #4: 5S rRNA
+Macromolecule #5: 5.8S rRNA
+Macromolecule #6: 18S rRNA
+Macromolecule #7: 60S ribosomal protein L2-A
+Macromolecule #8: Large ribosomal subunit protein uL3
+Macromolecule #9: 60S ribosomal protein L4-A
+Macromolecule #10: 60S ribosomal protein L5
+Macromolecule #11: 60S ribosomal protein L6
+Macromolecule #12: 60S ribosomal protein L7
+Macromolecule #13: 60S ribosomal protein L8
+Macromolecule #14: 60S ribosomal protein L9-B
+Macromolecule #15: 60S ribosomal protein L10
+Macromolecule #16: 60S ribosomal protein L11-A
+Macromolecule #17: 60S ribosomal protein L13
+Macromolecule #18: Large ribosomal subunit protein eL14 domain-containing protein
+Macromolecule #19: Ribosomal protein L15
+Macromolecule #20: 60S ribosomal protein L16-B
+Macromolecule #21: 60S ribosomal protein L17-B
+Macromolecule #22: 60S ribosomal protein L18-A
+Macromolecule #23: Ribosomal protein L19
+Macromolecule #24: 60S ribosomal protein L20-A
+Macromolecule #25: 60S ribosomal protein L21-A
+Macromolecule #26: 60S ribosomal protein L22
+Macromolecule #27: 60S ribosomal protein L23-B
+Macromolecule #28: 60S ribosomal protein L24
+Macromolecule #29: Large ribosomal subunit protein uL23
+Macromolecule #30: 40S ribosomal protein S8
+Macromolecule #31: 40S ribosomal protein S9-A
+Macromolecule #32: 40S ribosomal protein S10-A
+Macromolecule #33: 40S ribosomal protein S11-A
+Macromolecule #34: 40S ribosomal protein S13
+Macromolecule #35: Small ribosomal subunit protein uS11
+Macromolecule #36: 40S ribosomal protein S15
+Macromolecule #37: Small ribosomal subunit protein uS9
+Macromolecule #38: 40S ribosomal protein S17-B
+Macromolecule #39: 40S ribosomal protein S18
+Macromolecule #40: 40S ribosomal protein S19-A
+Macromolecule #41: Small ribosomal subunit protein uS10
+Macromolecule #42: 40S ribosomal protein S21
+Macromolecule #43: 40S ribosomal protein S22
+Macromolecule #44: 40S ribosomal protein S23
+Macromolecule #45: 40S ribosomal protein S24
+Macromolecule #46: 40S ribosomal protein S25
+Macromolecule #47: 40S ribosomal protein S26
+Macromolecule #48: 40S ribosomal protein S27
+Macromolecule #49: 40S ribosomal protein S28-A
+Macromolecule #50: Small ribosomal subunit protein uS14
+Macromolecule #51: Small ribosomal subunit protein eS30
+Macromolecule #52: Small ribosomal subunit protein RACK1
+Macromolecule #53: Ribosomal protein L24
+Macromolecule #54: 60S ribosomal protein L27
+Macromolecule #55: 60S ribosomal protein L28
+Macromolecule #56: 60S ribosomal protein L29
+Macromolecule #57: 60S ribosomal protein L30
+Macromolecule #58: 60S ribosomal protein L31-A
+Macromolecule #59: 60S ribosomal protein L32
+Macromolecule #60: 60S ribosomal protein L33-A
+Macromolecule #61: 60S ribosomal protein L34-B
+Macromolecule #62: 60S ribosomal protein L35
+Macromolecule #63: 60S ribosomal protein L36
+Macromolecule #64: Ribosomal protein L37
+Macromolecule #65: Large ribosomal subunit protein eL38
+Macromolecule #66: Large ribosomal subunit protein eL39
+Macromolecule #67: Large ribosomal subunit protein eL40
+Macromolecule #68: Small ribosomal subunit protein eS32
+Macromolecule #69: 60S ribosomal protein L44
+Macromolecule #70: 60S ribosomal protein L43
+Macromolecule #71: Small ribosomal subunit protein uS2
+Macromolecule #72: Small ribosomal subunit protein eS1
+Macromolecule #73: Rps21 ribosomal protein
+Macromolecule #74: Small ribosomal subunit protein uS3
+Macromolecule #75: 40S ribosomal protein S4
+Macromolecule #76: 40S ribosomal protein uS7 (formerly S5)
+Macromolecule #77: 40S ribosomal protein S6
+Macromolecule #78: 40S ribosomal protein eS7 (formerly S7)
+Macromolecule #79: GENETICIN
+Macromolecule #80: MAGNESIUM ION
+Macromolecule #81: SPERMIDINE
+Macromolecule #82: 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethy...
+Macromolecule #83: ZINC ION
+Macromolecule #84: water
-Experimental details
-Structure determination
| Method | cryo EM |
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Processing | single particle reconstruction |
| Aggregation state | particle |
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Sample preparation
| Buffer | pH: 7.4 |
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| Vitrification | Cryogen name: ETHANE-PROPANE |
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Electron microscopy
| Microscope | TFS KRIOS |
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| Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2 |
| Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
| Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm |
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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About Yorodumi



Keywords
Candidozyma auris (fungus)
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Processing
FIELD EMISSION GUN


