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- EMDB-55029: Ribosome-dome complex including the Sec-translocon (SecYEG-SecA-S... -

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Basic information

Entry
Database: EMDB / ID: EMD-55029
TitleRibosome-dome complex including the Sec-translocon (SecYEG-SecA-SecDF) from antibiotics treated Mycoplasma pneumoniae cells (Combined)
Map data
Sample
  • Cell: pooled Ribosome-Sec translocon-Dome supercomplexes from four antibiotic treated Mycoplasma pneumoniae datasets(chloramphenicol treated & on K2 camera; puseudoridimycin treated; spectinomycin treated; chloramphenicol treated & on K3 camera)
  • Protein or peptide: x 59 types
  • RNA: x 8 types
  • Ligand: x 5 types
KeywordsRibosome / Translocation / Chaperone / Antibiotics / TRANSLATION
Function / homology
Function and homology information


cell envelope Sec protein transport complex / protein-exporting ATPase activity / protein-secreting ATPase / intracellular protein transmembrane transport / protein transport by the Sec complex / protein-transporting ATPase activity / protein import / protein secretion / transmembrane protein transporter activity / protein targeting ...cell envelope Sec protein transport complex / protein-exporting ATPase activity / protein-secreting ATPase / intracellular protein transmembrane transport / protein transport by the Sec complex / protein-transporting ATPase activity / protein import / protein secretion / transmembrane protein transporter activity / protein targeting / transferase activity / large ribosomal subunit / ribosomal small subunit assembly / ribosomal small subunit biogenesis / 5S rRNA binding / small ribosomal subunit rRNA binding / ribosomal large subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / tRNA binding / cytoplasmic translation / negative regulation of translation / rRNA binding / ribosome / translation / ribonucleoprotein complex / structural constituent of ribosome / response to antibiotic / mRNA binding / RNA binding / zinc ion binding / ATP binding / membrane / plasma membrane / cytosol / cytoplasm
Similarity search - Function
: / Family of unknown function (DUF6856) / Protein of unknown function DUF3713 / Protein of unknown function (DUF3713) / Preprotein translocase SecG subunit / SecE subunit of protein translocation complex, bacterial-like / Protein translocase subunit SecA / SecA DEAD-like, N-terminal / SecA Wing/Scaffold / SecA, preprotein cross-linking domain ...: / Family of unknown function (DUF6856) / Protein of unknown function DUF3713 / Protein of unknown function (DUF3713) / Preprotein translocase SecG subunit / SecE subunit of protein translocation complex, bacterial-like / Protein translocase subunit SecA / SecA DEAD-like, N-terminal / SecA Wing/Scaffold / SecA, preprotein cross-linking domain / SecA motor DEAD / SecA conserved site / SecA, Wing/Scaffold superfamily / SecA, preprotein cross-linking domain superfamily / SecA, C-terminal helicase domain / SecA preprotein cross-linking domain / SecA Wing and Scaffold domain / SecA DEAD-like domain / SecA P-loop domain / SecA family signature. / SecA family profile. / SecA DEAD-like domain / SecA preprotein cross-linking domain / Protein translocase subunit SecY / Protein secY signature 1. / Protein secY signature 2. / SecY/SEC61-alpha family / SecY domain superfamily / SecY conserved site / SecY / Ribosomal protein uL10 C-terminal region / Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / Ribosomal protein L10 / : / : / : / Ribosomal protein uL15, N-terminal region / Ribosomal protein S14, type Z / Ribosomal protein L11, bacterial-type / Ribosomal protein L31 type A / Ribosomal protein L31 signature. / Ribosomal protein L11, conserved site / Ribosomal protein L11 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein S21 / Ribosomal protein L9 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L6, conserved site / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L6 signature 1. / Ribosomal protein L9, C-terminal domain superfamily / : / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, RNA binding domain / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L17 signature. / Ribosomal protein L36 signature. / : / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L32p, bacterial type / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L9 / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S13, bacterial-type / : / Ribosomal protein S19, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L5, bacterial-type
Similarity search - Domain/homology
Small ribosomal subunit protein bS16 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein bS21 / Uncharacterized protein MG055 homolog / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS15 ...Small ribosomal subunit protein bS16 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein bS21 / Uncharacterized protein MG055 homolog / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein uL10 / Uncharacterized lipoprotein MG348 homolog / Uncharacterized lipoprotein MG338 homolog / Uncharacterized lipoprotein MG309 homolog / Uncharacterized lipoprotein MG307 homolog / Uncharacterized protein MG277 homolog / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein uL11 / Protein translocase subunit SecA / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL33A / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL21 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL17 / Protein translocase subunit SecY / Probable protein-export membrane protein SecG
Similarity search - Component
Biological speciesMycoplasmoides pneumoniae M129 (bacteria)
Methodsubtomogram averaging / cryo EM / Resolution: 9.4 Å
AuthorsXue L / Jensen RK / Mahamid J
Funding support United States, Germany, China, Denmark, 4 items
OrganizationGrant numberCountry
Chan Zuckerberg Initiative2021-234620 United States
German Research Foundation (DFG)426290502 Germany
Ministry of Science and Technology (MoST, China)2024YFA1306200 China
Independent Research Fund Denmark - Medical Sciences0164-00010A Denmark
CitationJournal: To Be Published
Title: In-cell discovery and characterization of a non-canonical bacterial protein translocation-folding complex
Authors: Jensen R / Xue L / Marotta F / Somody JC / Selkrig J / Lenz S / Rappsilber J / Savitski MM / Kosinski J / Typas A / Zimmermann-Kogadeeva M / Bork P / Mahamid J
History
DepositionSep 7, 2025-
Header (metadata) releaseSep 23, 2026-
Map releaseSep 23, 2026-
UpdateSep 23, 2026-
Current statusSep 23, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_55029.png

Downloads & links

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Map

FileDownload / File: emd_55029.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
2.9 Å/pix.
x 256 pix.
= 742.4 Å		2.9 Å/pix.
x 256 pix.
= 742.4 Å		2.9 Å/pix.
x 256 pix.
= 742.4 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 2.9 Å
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Histogram (log scale)
Contour LevelBy AUTHOR: 0.62
Minimum - Maximum-1.1255736 - 3.1521726
Average (Standard dev.)0.012433072 (±0.13694802)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 742.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_55029_msk_1.map
Projections & Slices
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Half map: #2

Fileemd_55029_half_map_1.map
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Half map: #1

Fileemd_55029_half_map_2.map
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Sample components

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Entire : pooled Ribosome-Sec translocon-Dome supercomplexes from four anti...

EntireName: pooled Ribosome-Sec translocon-Dome supercomplexes from four antibiotic treated Mycoplasma pneumoniae datasets(chloramphenicol treated & on K2 camera; puseudoridimycin treated; spectinomycin ...Name: pooled Ribosome-Sec translocon-Dome supercomplexes from four antibiotic treated Mycoplasma pneumoniae datasets(chloramphenicol treated & on K2 camera; puseudoridimycin treated; spectinomycin treated; chloramphenicol treated & on K3 camera)
Components
  • Cell: pooled Ribosome-Sec translocon-Dome supercomplexes from four antibiotic treated Mycoplasma pneumoniae datasets(chloramphenicol treated & on K2 camera; puseudoridimycin treated; spectinomycin treated; chloramphenicol treated & on K3 camera)
  • Protein or peptide: Large ribosomal subunit protein bL34
  • Protein or peptide: 50S ribosomal protein L35
  • Protein or peptide: Protein translocase subunit SecY
  • Protein or peptide: Probable protein-export membrane protein SecG
  • Protein or peptide: SecE
  • Protein or peptide: SecDF
  • Protein or peptide: 50S ribosomal protein L36
  • Protein or peptide: Protein translocase subunit SecA
  • Protein or peptide: 30S ribosomal protein S2
  • Protein or peptide: 30S ribosomal protein S5
  • Protein or peptide: 50S ribosomal protein L31
  • Protein or peptide: 30S ribosomal protein S4
  • Protein or peptide: 30S ribosomal protein S6
  • Protein or peptide: 30S ribosomal protein S7
  • Protein or peptide: 30S ribosomal protein S8
  • Protein or peptide: Small ribosomal subunit protein uS9
  • Protein or peptide: 30S ribosomal protein S10
  • Protein or peptide: 30S ribosomal protein S11
  • Protein or peptide: 30S ribosomal protein S12
  • Protein or peptide: 30S ribosomal protein S13
  • Protein or peptide: 30S ribosomal protein S14 type Z
  • Protein or peptide: 30S ribosomal protein S15
  • Protein or peptide: 30S ribosomal protein S16
  • Protein or peptide: 30S ribosomal protein S17
  • Protein or peptide: 30S ribosomal protein S18
  • Protein or peptide: 30S ribosomal protein S19
  • Protein or peptide: 30S ribosomal protein S20
  • Protein or peptide: 30S ribosomal protein S21
  • Protein or peptide: 30S ribosomal protein S3
  • Protein or peptide: Nascent peptide
  • Protein or peptide: 50S ribosomal protein L2
  • Protein or peptide: 50S ribosomal protein L3
  • Protein or peptide: 50S ribosomal protein L4
  • Protein or peptide: 50S ribosomal protein L5
  • Protein or peptide: 50S ribosomal protein L6
  • Protein or peptide: 50S ribosomal protein L9
  • Protein or peptide: 50S ribosomal protein L10
  • Protein or peptide: 50S ribosomal protein L11
  • Protein or peptide: 50S ribosomal protein L13
  • Protein or peptide: 50S ribosomal protein L14
  • Protein or peptide: 50S ribosomal protein L15
  • Protein or peptide: 50S ribosomal protein L16
  • Protein or peptide: 50S ribosomal protein L17
  • Protein or peptide: Large ribosomal subunit protein uL18
  • Protein or peptide: 50S ribosomal protein L19
  • Protein or peptide: 50S ribosomal protein L20
  • Protein or peptide: 50S ribosomal protein L21
  • Protein or peptide: 50S ribosomal protein L22
  • Protein or peptide: Large ribosomal subunit protein uL23
  • Protein or peptide: 50S ribosomal protein L24
  • Protein or peptide: 50S ribosomal protein L27
  • Protein or peptide: 50S ribosomal protein L28
  • Protein or peptide: 50S ribosomal protein L29
  • Protein or peptide: 50S ribosomal protein L32
  • Protein or peptide: 50S ribosomal protein L33 1
  • Protein or peptide: Uncharacterized lipoprotein MG307 homolog
  • Protein or peptide: Uncharacterized lipoprotein MG309 homolog
  • Protein or peptide: Uncharacterized lipoprotein MG338 homolog
  • Protein or peptide: Uncharacterized lipoprotein MG348 homolog
  • RNA: 23S ribosomal RNA
  • RNA: 5S ribosomal RNA
  • RNA: 16S ribosomal RNA
  • RNA: 16S ribosomal RNA
  • RNA: tRNA-Asp (P-site)
  • RNA: tRNA-Lys (A-site)
  • RNA: mRNA
  • RNA: mRNA
  • Ligand: ZINC ION
  • Ligand: MAGNESIUM ION
  • Ligand: 1,4-DIAMINOBUTANE
  • Ligand: POTASSIUM ION
  • Ligand: water

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Supramolecule #1: pooled Ribosome-Sec translocon-Dome supercomplexes from four anti...

SupramoleculeName: pooled Ribosome-Sec translocon-Dome supercomplexes from four antibiotic treated Mycoplasma pneumoniae datasets(chloramphenicol treated & on K2 camera; puseudoridimycin treated; spectinomycin ...Name: pooled Ribosome-Sec translocon-Dome supercomplexes from four antibiotic treated Mycoplasma pneumoniae datasets(chloramphenicol treated & on K2 camera; puseudoridimycin treated; spectinomycin treated; chloramphenicol treated & on K3 camera)
type: cell / ID: 1 / Parent: 0
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)

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Macromolecule #1: Large ribosomal subunit protein bL34

MacromoleculeName: Large ribosomal subunit protein bL34 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 5.616773 KDa
SequenceString:
MKRTYQPSKL KRAKTHGFLA RMATASGRKV LKLRRKKQRA QLTVSSER

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #2: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 6.793135 KDa
SequenceString:
MKVKSAAKKR FKLTKSGQIK RKHAYTSHLA PHKTTKQKRH LRKQGTVSAS DFKRIGNLI

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #3: Protein translocase subunit SecY

MacromoleculeName: Protein translocase subunit SecY / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 52.003633 KDa
SequenceString: MQAKPTTAVK QKQNFGQRLF TLLRNRDFMI SFLITVVLLV LFRVLAIIPL PGIQVNQTGL DQNSNDFFSL FNLLGGGGLN QLSLFAVGI SPYISAQIVM QLLSTDLIPP LSKLVNSGEV GRRKIEMITR IITLPFALVQ SFAVIQIATN SGGGSSPITL K NNGSDFVA ...String:
MQAKPTTAVK QKQNFGQRLF TLLRNRDFMI SFLITVVLLV LFRVLAIIPL PGIQVNQTGL DQNSNDFFSL FNLLGGGGLN QLSLFAVGI SPYISAQIVM QLLSTDLIPP LSKLVNSGEV GRRKIEMITR IITLPFALVQ SFAVIQIATN SGGGSSPITL K NNGSDFVA FYIIAMTAGT YLSVFLGDTI SKKGIGNGIT LLILSGILAQ LPEGFIAAYS VLSGVVVTIN ATLTTAISFF IY FMAFVTL LFATTFITQE TRKIPIQQSG QGLVTESSAL PYLPIKVNSA GVIPVIFASS IMSIPVTIAQ FQPQTESRWF VED YLSLSK PTGIVLYGIL VILFSFFYSY IQINPERLAK NFEKSGRFIP GIRPGKDTEK HIARVLVRIN FIGAPFLTVI AIIP YIVSA LIHLPNSLSL GGTGIIIIVT AVVEFMSALR SAATATNYQQ LRRNLAIEVQ KTAQQDKEEQ LRAETPGIGN LW

UniProtKB: Protein translocase subunit SecY

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Macromolecule #4: Probable protein-export membrane protein SecG

MacromoleculeName: Probable protein-export membrane protein SecG / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 8.352255 KDa
SequenceString:
MDAIQIVMFV MAILCLIIGL LLSNHGSTGG LASLSGQDLE IFRKTKDRGI VKILQITMFI LVVLFLILGL VFHFAL

UniProtKB: Probable protein-export membrane protein SecG

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Macromolecule #5: SecE

MacromoleculeName: SecE / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 14.489404 KDa
SequenceString:
MEKKKLPFGL KNKEKLTAYN DEKIHELHRQ LKAKIEAKKA KEKQDSKTKD TDKKVDQTPK VKVPFTKKFS NLWFGIDKEV NKIVWVTSK KLITIFLLIV LVSAIMIGIY FGINHLFIAL GVFKGK

UniProtKB: Uncharacterized protein MG055 homolog

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Macromolecule #6: SecDF

MacromoleculeName: SecDF / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 108.345633 KDa
SequenceString: MGLKKRVSFD WLLKIGIILV LAFLGLFGII FGSYKLLNDS RLGVVFNGST TTTVYFLNHK SKDANKELDP TQKKDGNGSG NNTEMITDV NGFLNNIEKS YANSLYAQGF SSVNITKNSH DTSAKELSDK GVFDSSWLVN DGLPSISVTF EQRREEARTR A RKRQIDAQ ...String:
MGLKKRVSFD WLLKIGIILV LAFLGLFGII FGSYKLLNDS RLGVVFNGST TTTVYFLNHK SKDANKELDP TQKKDGNGSG NNTEMITDV NGFLNNIEKS YANSLYAQGF SSVNITKNSH DTSAKELSDK GVFDSSWLVN DGLPSISVTF EQRREEARTR A RKRQIDAQ VKRNALSAIE HNYKLSLETT DGIVLFDSFD KEFIRNSLTA VVPQTTNTNS ALTFEYKLSK NVITKESLHN DF LDFIKSK SLTDSDYNTG NGQKSVEGSG KWFKDKANGS AQNGNKTLVL WKDKEGAVQY VRNIFNVPKG GTDYLTFNER EKN LWDFLH AEGNFSSGDN LFLQKNNNGN SPITKASDIT LKNIYYIYAA PTHFSQVATK DNNNKDAADV VAVANSADTR KLRS GDATG FSGLFSNYIL AEIRTEDPVA KNGDPVKVNA TLQSFLDSNN QRLEHGGNIK FQVANFVNND GSYVPASYLE ASRVK VLLS NGFPETATIA AANTKLVNAP LSNTLARDVS NFASSFIALG VIILLATIAL GIRYKLLGLY KALALALSAI SSLAFF SAV GGVVDVFSFV GIFFVVAVNI INLITLAELF LRNIKNNASI VESWKLCLKR SFFTMIETHI CWLLSALVVI YLSNYQV QQ LGTLMAVSAI TSYFLNYGLG VILVWLFVSS HSGLEWRFFL YKKDAQALTK TSSNYSILTE NNDIQTDFFI SKNQHDFF G KKKWSLLFIW LTVLAFGVVM LGLYLGAPQL LGNFLAADIE SSTGIMLGVG VISLLYLLYS LPRYGVAYGI SYLIALILL AAGLFGAMYL ANFIFRIDQS TIQLIIFVYL FWLFFQARIG QTTIWTYCYW FKRSLKDRVF VKNLFNDNVN SQWRLDLIGS SVLILLFIV YAAFNFGGIN GTINLVIFYL IAIVALFSVF VNGLPLFSFG LINGWLSPYN YVQIHITLRH KKQMFKEVDQ I EEQLISGI NSF

UniProtKB: Uncharacterized protein MG277 homolog

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Macromolecule #7: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 4.400488 KDa
SequenceString:
MKVRASVKPI CKDCKIIKRH QIVRVICKTQ KHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #14: Protein translocase subunit SecA

MacromoleculeName: Protein translocase subunit SecA / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO / EC number: protein-secreting ATPase
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 91.942234 KDa
SequenceString: MGLFNFLKLV SPRHRIYHKA SKIANEVEGH KNYYRNLTDV QLLEESNKLV DLVTKQNYTI LDVAVAALAL IREVVYRETG EFAYRVQII GAYIVLIGDF AEMMTGEGKT LTIVLAAYVS ALEKRGVHVV TVNEYLAQRD ATNATKILKR VGMTVGCNFA N LAPHLKQA ...String:
MGLFNFLKLV SPRHRIYHKA SKIANEVEGH KNYYRNLTDV QLLEESNKLV DLVTKQNYTI LDVAVAALAL IREVVYRETG EFAYRVQII GAYIVLIGDF AEMMTGEGKT LTIVLAAYVS ALEKRGVHVV TVNEYLAQRD ATNATKILKR VGMTVGCNFA N LAPHLKQA AFACDVTYTT NSELGFDYLR DNMVHRFEDK KIRELHFAIV DEGDSVLIDE ARTPLIISGP AKNEFAAYVA VD RFVKKLK EDEYKIDPES RAPALTELGI KHAEKNFKTD NLFALENSDL FHKIINALTA VKVFEQGKEY IVRDGKVLIV DHF TGRILE GRSYSNGLHQ AVQAKEMVEI EPENVIVATI TYQSFFRLYN RLSAVSGTAF TESEEFLKIY NMVVVPVPTN RPNI RKDRA DSVFGTPNIK WLAVVKEVKR IHETGRPILI GTANIDDSEL LHNYLQEANI PHEVLNAKNH SREAEIVAKA GQKGA VTIS TNMAGRGTDI RLGEGVAEMG GLYVLGTERN ESRRIDNQLR GRAGRQGDRG ETKFFISLGD ALFKRFAHDR IERAIT KLG NDTFDSSFFS KMLSRTQKRV EAINFDTRKN LIDYDHVLAS QRELIYKQRD KFLLATDLSD MIDKMLEKFV EQFCDQY RN PKNQNLVNHI ALSEALNLEL NMHGVISPKL FENMTFDATV HKTHSLIGEK ITNKVKVLTP PIALIRFREI MITAMDKH W IEHLDNVFKL REGVTLRSME QTSPLNVYIR ETDILFQTML QKIARDVIIQ IANLATPEEF DEELMKANAL KKLQALREA HEKSNEGQ

UniProtKB: Protein translocase subunit SecA

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Macromolecule #15: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 33.468629 KDa
SequenceString: MSELITTPVE TTAKAELVSL AKLGEMRTHV GMVKRYWNPK MGFFIEPERK HNNDHFVLEL QRQSLQTAYN YVKEVAQNNG QILFVGTKN DYVKKLVNNI AKRVDVAFIT QRWLGGTLTN FKTLSISINK LNKLVEKQAE NAADLTKKEN LMLSREIERL E KFFGGVKS ...String:
MSELITTPVE TTAKAELVSL AKLGEMRTHV GMVKRYWNPK MGFFIEPERK HNNDHFVLEL QRQSLQTAYN YVKEVAQNNG QILFVGTKN DYVKKLVNNI AKRVDVAFIT QRWLGGTLTN FKTLSISINK LNKLVEKQAE NAADLTKKEN LMLSREIERL E KFFGGVKS LKRLPNLLIV DDPVYEKNAV AEANILRIPV VALCNTNTNP ELVDFIIPAN NHQPQSTCLL MNLLADAVAE AK AMPTMFA YKPDEEIQIE IPQKQEAPRQ VVNRANSKQI TSQRLNITRN PEVLTRE

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #16: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 24.138008 KDa
SequenceString: MTDQNQKANQ GNGLQTTNLQ AHAQRKHNLR PSSEGIKKAV SKKEGGGHNR NNQNRRFQKP AFKSEFEERI VKLKRISKTT KGGRNMRFS VLVVVGNRKG KIGYGIAKAL EVPNAIKKAI KAAHNSLHTI EIHKGSIYHE VIGRSGASRV LLKPAPQGTG I IAGGAIRA ...String:
MTDQNQKANQ GNGLQTTNLQ AHAQRKHNLR PSSEGIKKAV SKKEGGGHNR NNQNRRFQKP AFKSEFEERI VKLKRISKTT KGGRNMRFS VLVVVGNRKG KIGYGIAKAL EVPNAIKKAI KAAHNSLHTI EIHKGSIYHE VIGRSGASRV LLKPAPQGTG I IAGGAIRA IIELAGYSDI YTKNLGRNTP INMIHATMDG ILKQLSPRRV AILRNKNLNE L

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #18: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 10.980657 KDa
SequenceString:
MKKDFHFPSQ SVSFKCASCS NSFTIESTLK QKEITIDICG KCHPFYIGEL TKQTVHGRAE KLSGKFNAGK AFLENKTPKK AKGKTEEYT KHRSLNEL

UniProtKB: Large ribosomal subunit protein bL31

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Macromolecule #19: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 23.817645 KDa
SequenceString: MKYTGSIFKR SRRLGFSLLE NNKEFSKGKK RKTIPGQHGN RFRSSTMSGY AQQLQEKQRM QYMYGITDKQ FRRLFRLVLK QRGNLAVNL FRVLESRLDN IVYRMGFAPT RRSARQLVNH GHVLLNDRTV DTPSIILNPG DKVRLKAKTI KIPIVKAASE S GVVSPFVE ...String:
MKYTGSIFKR SRRLGFSLLE NNKEFSKGKK RKTIPGQHGN RFRSSTMSGY AQQLQEKQRM QYMYGITDKQ FRRLFRLVLK QRGNLAVNL FRVLESRLDN IVYRMGFAPT RRSARQLVNH GHVLLNDRTV DTPSIILNPG DKVRLKAKTI KIPIVKAASE S GVVSPFVE TNNKTFEGTY VRFPERSELP AGINESYVVE WYKRLVK

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #20: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 25.430465 KDa
SequenceString: MQYNIILLVD GSLSLEQANQ VNEKQQQTLT NVEGLQTEYL GLKELAYPIK KQLSAHYYRW KFSGDNQSTK DFKRTANINK QVLRELIIN LEREYGYLAS INPKKQQLAL QKRAKYDEII ARENNPENPD VPVTSGLAST QPRLSRTEKA QKPKEELWDV V QKMGNFDS ...String:
MQYNIILLVD GSLSLEQANQ VNEKQQQTLT NVEGLQTEYL GLKELAYPIK KQLSAHYYRW KFSGDNQSTK DFKRTANINK QVLRELIIN LEREYGYLAS INPKKQQLAL QKRAKYDEII ARENNPENPD VPVTSGLAST QPRLSRTEKA QKPKEELWDV V QKMGNFDS VQANPYRPRF KRFNAEHVNQ RQNQQNNNNN RFDRNRNRQH NRFKDKQ

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #21: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 17.897029 KDa
SequenceString:
MRKNRAPKRT VLPDPVFNNT LVTRIINVIM EDGKKGLAQR ILYGAFDLIE QRTKEKPLTV FERAVGNVMP RLELRVRRIA GSNYQVPTE VPQDRKIALA LRWIAMFARK RHEKTMLEKI ANEIIDASNN TGAAIKKKDD THKMAEANKA FAHMRW

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #22: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 15.903083 KDa
SequenceString:
MITTTKPIKA HFDPVADLLT KINNARKAKL MTVTTIASKL KIAILEILVK EGYLANFQVL ENKSKTKRIV TFNLKYTQRR IPSINGVKQ ISKPGLRIYR PFEKLPLVLN GLGIAIISTS DGVMTDKVAR LKKIGGEILA YVW

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #23: Small ribosomal subunit protein uS9

MacromoleculeName: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 15.158702 KDa
SequenceString:
MEKQSYYGLG RRKSSSAKVY LTPTQDHGKI TVNRRDPSEY FPNKLVIQDM EQPLDLTDLK KNFDINVVVK GGGFTGQAGA IRLGIVRAL LQFNPELKKI LKSKKLTTRD KRVKERKKFG LYGARRAPQF TKR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #24: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 12.226571 KDa
SequenceString:
MNAANAVKYP ELKIKLESYD STLLDLTTKK IVEVVKGVDV KIKGPLPLPT KKEVITIIRS PHVDKASREQ FEKNRHKRLM ILVDVNQGA IDSLKRIKIP VGVTLRFSK

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #25: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 12.709851 KDa
SequenceString:
MAKKKKINVS SGIIHVSCSP NNTIVSASDP GGNVLCWASS GTMGFKGSRK KTPYSAGIAA DKVAKTVKEM GMATVKLFVK GTGRGKDTA IRSFANAGLS ITEINEKTPI PHNGCKPPKR PR

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #26: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 15.66658 KDa
SequenceString:
MATIAQLIRK PRKKKKVKSK SPALHYNLNL LNKKVTNVYS PLKRGVCTRV GTMTPKKPNS ALRKYAKVRL TNGFEVLTYI PGEGHNLQE HSVTLLRGGR VKDLPGVRYH IVRGTLDTVG VEKRRQQRSA YGAKKPKAKS

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #27: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 14.209607 KDa
SequenceString:
MARILGIDIP NQKRIEIALT YIFGIGLSRS QAILKQANIN PDKRVKDLTE EEFVAIRNVA SAYKIEGDLR REIALNIKHL SEIGAWRGL RHRKNLPVRG QRTRTNARTR KGPRKTVANK KIESK

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #28: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 6.901364 KDa
SequenceString:
MAKKSLKVKQ TRIPKFAVRA YTRCQRCGRA RAVLSHFGVC RLCFRELAYA GAIPGVKKAS W

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #29: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 9.921687 KDa
SequenceString:
MQIDKNGIIK SAQLHDKDVG SIQVQVSLLT SQIKQLTDHL LANKKDFISK RGLYAKVSKR KRLLKYLKHN DLEAYRNLVK TLNLRG

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #30: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 10.806921 KDa
SequenceString:
MVKIRLMRMG RVHYPTYRIV AVDSRVKRDG KYIALIGHLN PALKENKCKI DEAVALEWLN KGAKPTDTVR SLFSQTGLWK KFVESKKKP VAKSK

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #31: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 9.859643 KDa
SequenceString:
MKRNQRKVLI GIVKSTKNAK TATVQVESRF KHPLYHKSVV RHKKYQAHNE GEVLAKDGDK VQIVETRPLS ATKRFRIAKI IERAK

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #32: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 12.411522 KDa
SequenceString:
MMNNEHDNFQ KEVETTTETT FNREEGKRMV RPLFKRSKKY CRFCAIGQLR IDLIDDLEAL KRFLSPYAKI NPRRITGNCQ MHQRHVAKA LKRARYLALV PFVKD

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #33: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 10.057626 KDa
SequenceString:
MSRSAKKGAF VDAHLLKKVI DMNKQEKKRP IKTWSRRSTI FPEFVGNTFA VHNGKTFINV YVTDDMVGHK LGEFSPTRNF KQHTANR

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #34: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 9.993599 KDa
SequenceString:
MANIKSNEKR LRQNIKRNLN NKGQKTKLKT NVKNFHKEIN LDNLGNVYSQ ADRLARKGII STNRARRLKS RNVAVLNKTQ VTAVEGK

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #35: 30S ribosomal protein S21

MacromoleculeName: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 7.539182 KDa
SequenceString:
MPKIEVKNDD LELALKKFKR VSLEIRRLAQ RHEYHLRKGM RLREKRKIAQ KKRRKFRNMV

UniProtKB: Small ribosomal subunit protein bS21

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Macromolecule #36: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 30.657205 KDa
SequenceString: MGQKVNSNGL RFGINKNWIS RWTANSHAQT AKWLIEDEKI RNLFFVNYRN AQVSNVEIER TQATVDVFVY AAQPAFLIGS ENKNIQKIT KQIKQIIGRT TNLDLTINEI GSPMLSARII ARDLANAIEA RVPLRTAMRQ SLIKVLKAGA NGIKVLVSGR L NGAEIARD ...String:
MGQKVNSNGL RFGINKNWIS RWTANSHAQT AKWLIEDEKI RNLFFVNYRN AQVSNVEIER TQATVDVFVY AAQPAFLIGS ENKNIQKIT KQIKQIIGRT TNLDLTINEI GSPMLSARII ARDLANAIEA RVPLRTAMRQ SLIKVLKAGA NGIKVLVSGR L NGAEIARD KMYIEGNMPL STLRADIDYA LEKAQTTYGV IGVKVWINRG MIYTKGLNRT PAHILHPQKK QPNRQNQQPR HF NQGQVLS ANKLTGSDVE TSSIQALTKP NKEDKQ

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #38: Nascent peptide

MacromoleculeName: Nascent peptide / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 3.127794 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) KAAD

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Macromolecule #39: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 31.966871 KDa
SequenceString: MPIKKIISRS NSGIHHSTVI DYKKLLTTNK NKPEKSLLVT LKKHGGRNNQ GKITVRHQGG RNKRKYRIID FKRTHYDNIE ATVKSIEYD PNRSCFVSLI TYANGAKSYI ISPDGIKVGD KILASEHPID IKPGFSMPLA FIPEGTQVHN IELHPKGGGQ I ARSAGSYA ...String:
MPIKKIISRS NSGIHHSTVI DYKKLLTTNK NKPEKSLLVT LKKHGGRNNQ GKITVRHQGG RNKRKYRIID FKRTHYDNIE ATVKSIEYD PNRSCFVSLI TYANGAKSYI ISPDGIKVGD KILASEHPID IKPGFSMPLA FIPEGTQVHN IELHPKGGGQ I ARSAGSYA RILGQDETGK YVILQLLSGE TRKFLKECRA TVGVVSNLDH NLVVIGKAGR NRHRGIRPTV RGSAMNPNDH PH GGGEGRS PVGRDAPRTP WGKRHMGVKT RNMKKASTNL IIRNRKGEQY

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #40: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 31.298172 KDa
SequenceString: MEIRGIFGVK VGMSQVFTTN NERLPITVIY CEPNQVAGVK TEAKDKYSAT LLSFDTVENK KLNKPQQGFF EKNNLKPTKH LQEIRNMTG FEMGQQITPQ NLFQVGEYVD VSAISKGRGF TGAIKRWNFK IGPLGHGAGY PHRFQGSVQA GRGGASAQRV F KGKKMSGH ...String:
MEIRGIFGVK VGMSQVFTTN NERLPITVIY CEPNQVAGVK TEAKDKYSAT LLSFDTVENK KLNKPQQGFF EKNNLKPTKH LQEIRNMTG FEMGQQITPQ NLFQVGEYVD VSAISKGRGF TGAIKRWNFK IGPLGHGAGY PHRFQGSVQA GRGGASAQRV F KGKKMSGH YGHEKVTVQN LRIVGFDEAN MLVLVSGAIA GPEGGVVLIR TAKKKPGVVK PIELAVQTEK APEAKPAKLS KK KQAKELA KAQAANQQTV EAKVDTPVVE PKPTEVKKAA PVVEKKGEDK

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #41: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 23.632572 KDa
SequenceString: MAKLKLIKID GSFETEPVKL SPGLIAKELK QQPVFDAVLV EQASWRQGTH SILTKGEVRG GGKKPYKQKH TGKARQGSTR NPHFVGGGI VFGPKPNRNY SLKLNKKAHT AALHTVWSEK LASDNTHLVD QNLFNKTEGK TKVMMQFLKS AKLLDKNVLF V VNTLNTNL ...String:
MAKLKLIKID GSFETEPVKL SPGLIAKELK QQPVFDAVLV EQASWRQGTH SILTKGEVRG GGKKPYKQKH TGKARQGSTR NPHFVGGGI VFGPKPNRNY SLKLNKKAHT AALHTVWSEK LASDNTHLVD QNLFNKTEGK TKVMMQFLKS AKLLDKNVLF V VNTLNTNL EQSTSNIKNV QVKHLDKVSV RDLMLANALL VEKEVLKALE GKFK

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #42: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 20.270834 KDa
SequenceString:
MNNLKAHYQK TIAKELQKSF AFSSIMQVPR LEKIVINMGV GDAIRDSKFL ESALNELHLI SGQKPVATKA KNAISTYKLR AGQLIGCKV TLRGERMWAF LEKLIYVALP RVRDFRGLSL KSFDGRGNYT IGIKEQIIFP EIVYDDIKRI RGFDVTLVTS T NKDSEALA LLRALNLPLV KG

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #43: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 20.624062 KDa
SequenceString:
MSKIGNRTIT LDPAKVNLNF QKDHIAVKGP LGQIELKLPP NLPLKFELKD NNLQITRNNE LKQSKIFHGT YNALITNAII GVTQGFEKK LRLVGVGYRA NVEGETLNLQ LGYSHPIKEK IPKGLTVKVE KNTEITISGI SKELVGQFAT EVRKWRKPEP Y KGKGVLYF DEVIVRKAGK TAEGKK

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #44: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 17.185037 KDa
SequenceString:
MKVILKQDVS NLGKRFDVVD VKDGYAIHFL FPKKLAAPLT KKSLQDRDLF LKKQQEHYEI NKALSHKLKE VIEQTELHFS LKEHNGRPY GSIITKQIIN QAHTKGMALQ KFMFKDNVRL GFGDHEITLH IFEDTTAVLK VKVTPDNGVK

UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #45: 50S ribosomal protein L10

MacromoleculeName: 50S ribosomal protein L10 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 17.645719 KDa
SequenceString:
MEAKKDKAQQ VADVSHLLST SAGFVIFDYT SMSAIEATSI RKKLFKNGSK IKVIKNNILR RALKAGKFEG IDETAIKGKL AVAVGVNEI VETLKAVDGV VKAKEAMNFV CGYFDNRAFN SADLEKIAKL PGRNELYGMF LSVLQAPLRK FLYALEAVKA A K

UniProtKB: Large ribosomal subunit protein uL10

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Macromolecule #46: 50S ribosomal protein L11

MacromoleculeName: 50S ribosomal protein L11 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 14.80361 KDa
SequenceString:
MAKKTITRIA KINLLGGQAK PGPALASVGI NMGEFTKQFN EKTKDKQGEM IPCVITAYND KSFDFILKTT PVSILLKQAA KLEKGAKNA KTIVGKITMA KAKEIAQYKL VDLNANTVEA ALKMVLGTAK QMGIEVIE

UniProtKB: Large ribosomal subunit protein uL11

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Macromolecule #47: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 16.821477 KDa
SequenceString:
MQKTSMLTKE QANKRRQWYI VDAAGLVLGK LAVKAADLIR GKNKVDFTPN QDCGDYLIII NSDQVVLTGN KKENEFWYHH SQYIGGIKK VSGRDMLKKQ SDKLVYNAVK GMLPDNRLSR RWITKVHVFK GDKHNMEAQK PTTLNWS

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #48: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 13.478906 KDa
SequenceString:
MVSFMTRLNV ADNTGAKQVG IIKVLGSTRK RYAFLGDVVV VSVKDAIPSG MVKKGQVLRA VIVRTKKGQQ RKDGTHLKFD DNACVLIKE DKSPRGTRIF GPVARELRER GYNKILSLAV EVV

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #49: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 16.768812 KDa
SequenceString:
MELNQLKSVP KARNHKTKTL GRGHGSGLGK TSGRGQKGQK ARKSGLTRPG FEGGQTPLYR RLPKFGNARK GFLKQEWVVL NLNKIAKLK LDKINRASLI EKQVISAKSQ LPIKLIGHTK LEKPLHFEVH KVSKQALKAV ENANGSVKLL EK

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #50: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 15.646479 KDa
SequenceString:
MLQPKRTKYR KPHNVSYEGK AKGNSYVAFG EYGLVATKGN WIDARAIESA RIAISKCLGK TGKMWIRIFP HMSKTKKPLE VRMGSGKGN PEFWVAVVKQ GTVMFEVANI PESQMIKALT RAGHKLPVTW KILKREEVSA

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #51: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 14.270727 KDa
SequenceString:
MSYINKPGKT SAWRVMTVRQ QVSAVLAYGK IETTLKKAKN TQKRLDKLIT LAKVDNFNNR RQVKKWLLNT NLFDVDQLMD HLFSKVAPK YEKTPGGYSR VLKLGPRRGD ATEMAILQLT DAKYK

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #52: Large ribosomal subunit protein uL18

MacromoleculeName: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 13.073256 KDa
SequenceString:
MKTRTEQRRL RHKRIVKKIR ATNHDNRVVL MVIKSLNHIS VQAWDFSQNI VLASSSSLAL KLKNGNKDNA KLVGQDIADK LVKLKLTNV VFDTGGSKYH GRIAALAEAA RERGLNF

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #53: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 13.870327 KDa
SequenceString:
MKKINKQALI DLVEQKQLKA YVPEFSAGDE VNVAIKLKEK EKVRIQNFTG TVLRRRGKGI SETFIVRKTT DGIPIEKNFQ IHNPNISIE LKRRGKVRRA YISYMRERSG KAAKIKERKQ

UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #54: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 14.742431 KDa
SequenceString:
MRIKGGKQTR VRRKKWLKQA SGSFGTRHAS YKVAKQTVIQ AAKYAYRDRR NKKRDFRSLW ILRLNAALRE QGMTYSVFIN LLKKHNIEI NRKVLSELAI KEPSKFNLIV QKVKSEQPKA AKPAALGN

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #55: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 11.664824 KDa
SequenceString:
MHAIVVCGSK QYLVHENDTF FVEKLEAPVG KEIQLDKVLM LDEKIGAPYL EKARVVCVVE KHGLQRKVNV IKHISQKHHL KKYGHRQPY TKLKVVRFVH D

UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #56: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 17.355402 KDa
SequenceString:
MIAFAKQFRV RISPQKARLV CQLIVGKKTA DAQNILSNTP KKAATLIAKL LNSAIANATN NHGMNGDALY VFECVANQGP SMKRTIPRA KGSSNMITKR SSNLVVKLSD NPNERQELIK QQKALVKKRV EGQQKAKMAR QKAVTSVVKA PSKTQGGVQK

UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #57: Large ribosomal subunit protein uL23

MacromoleculeName: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 21.139135 KDa
SequenceString: MDVTNVLLKP VLTEKVYFNQ MGETKKYVFV VNPKASKTRV KLAFELVYGI KPLKVNTLIR KPTTIRGGSR FPGLSKLEKL AVITLPKGI AISVTGEAPE KTDKPADKTT LKESTVKEIK DTKNSPEAVV KTAVEALQIK PTAAPVTTAP LQTVAVKVAK E VKEVKVEK ...String:
MDVTNVLLKP VLTEKVYFNQ MGETKKYVFV VNPKASKTRV KLAFELVYGI KPLKVNTLIR KPTTIRGGSR FPGLSKLEKL AVITLPKGI AISVTGEAPE KTDKPADKTT LKESTVKEIK DTKNSPEAVV KTAVEALQIK PTAAPVTTAP LQTVAVKVAK E VKEVKVEK PVKVEKPTKP AKVAKEAKTT KVAKET

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #58: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 12.461865 KDa
SequenceString:
MQRIKKGDKV VVITGKNKGG SGIVLKIMPA RQQAIVEGLN KVTRHKKKDQ TTKRAAKQST GKVQQEAPIF LSKLALFDQK AKQQTIGKI KYVMDPKTNK KTRVFKKSNN TL

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #59: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 11.527338 KDa
SequenceString:
MNNKYFLTKI DLQFFASKKG VGSTKNGRDS HAKRLGAKKA DGQMIRTGQI IYRQRGTRVY PGVNVGLGSD DTLFALSDGL VKYQKFGPK QGKTRVSVVK HKLDA

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #60: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 7.549902 KDa
SequenceString:
MAKKDQLTLR GPLYGNNRSH SKTITRRKWN VNLQPCKVKT ADGKTTRILV STRTLRTLKK HNRLS

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #61: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 13.003328 KDa
SequenceString:
MTVAKELRQK SSEELVKLVI KLKGELLEYR FKLAHGELDK PHLINQTRRL LATILTILTE RKLNWQEEQA KYKLLTKKTN EAAVNAWKQ HLEANKAKLL KSRAKREDAS KK

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #62: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 6.641897 KDa
SequenceString:
MAVQQRRSSK HRRDKRRSHD ALTAQALSVC QKCGKKKLFH RVCSCGMYGD LRVKKAY

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #63: 50S ribosomal protein L33 1

MacromoleculeName: 50S ribosomal protein L33 1 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 6.298612 KDa
SequenceString:
MAVKRSTRLG CNDCREINYL TFKNVKKNPE KLALNKFCSR CRKVVVHKEV KRK

UniProtKB: Large ribosomal subunit protein bL33A

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Macromolecule #64: Uncharacterized lipoprotein MG307 homolog

MacromoleculeName: Uncharacterized lipoprotein MG307 homolog / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 136.216672 KDa
SequenceString: CATPSNSALQ TVFKARSSQF FNGEQGSLQS ALTTALKNPV ANKQFIAAPL LKALEAWYEN NEDKKITQFL KDTKSNVDSQ YTTAVDKVV SASRNKSLFV QQDLLDNAGG SEATWKAQKL LEQLISDFAS RVFQKNYLNY KKDGQVSTGP FTYDELHKEE S WKNFEFSA ...String:
CATPSNSALQ TVFKARSSQF FNGEQGSLQS ALTTALKNPV ANKQFIAAPL LKALEAWYEN NEDKKITQFL KDTKSNVDSQ YTTAVDKVV SASRNKSLFV QQDLLDNAGG SEATWKAQKL LEQLISDFAS RVFQKNYLNY KKDGQVSTGP FTYDELHKEE S WKNFEFSA PRFSETNDDF FAKIQSQVFD QWVEYTDPTL ISQVNYKYSA PSQGLGQIYN REKLKDKLTP SYAFPFFAEE KD IAPNQNV GNKRWKQLVK GEGAITDNNI GQSGTNSQKT GLLKYRNESN KGDFLDFPLN LSDTNETKQL VDASNIVDQL EAA NLGAAL NLKLQVFEQD NDELPQIKEL KEDLNNTIVV DKSKDVEKAS KTNALFYNDQ EGKQQQSDSD PIAGALDDIF AQNT SEGTN LSKLAEQVKK AAATKMEAKT AVLRTNNSKG QQNNYVVLDA AIPTFNSTTS KSKNNSASNE VLVALKSGSI NLRQV QQTD QNSYSPIKFR IVRNSTGVTV FGLDGGSYYL KQDSTNKKSV SKQSLTLLTK SSSGNSNKVL RDLDKQKQFL KFRAFQ AKT NTFYSTNFAF SFPLNETLKS WFDKHRELIL ANALVNASLD QKDKASKALT EAFNPYKELI KEFAPVALAT TMISFYF DQ MKALNNKLLE RARNLNQNVN QANPTPWLNG LSAKLPYVNT NGNYEKLNNY FTFLITKTLW PKVGQEETSI SEESNKLK T KTADVDKIRD KILENIQTKV NDFVKNKLKP ALAPRPAYSN VILLNVNNDK VLSSGANWSL ASLLQSDKVN PLSFMLLKQ AFDNNDLFKK AQKLFKDIQE KSSNNGGMQS SSTTNSDADA LSKVIGNYYY TTWAKLTDKS IYGNPKDNKF DELFKLAFEA SIDEKSFNV DYKAVIDHYR FIYTLQWLVD QKLKNFKSLL KTNLKFGEVA FIAYKNTETT NFSNPQGVFG SYFNYENSAS E VKESTQTL DPNNFFYKTT TKPTVQAIQQ VASLALVQKQ QMQQNSTDHY GFTGLSTSTS SMFDASSRDA ILQQITKTSL QQ YGSKDQL KKIIQGTNNQ LLLDRIAVQL SGLNPSTTNG GSGKTIATYF QVDAVGNPTL DFQAKRKLLL DLLDQYQNYF GNG AQKSQR DSTPSGTGNY LTYQNGSDKY TYTQFTYQDI DSLSLTTTSG TNNKIASDVV AALLLFQAAD KGTQQLALSA INKP QLNIG DKRIESGLKL LK

UniProtKB: Uncharacterized lipoprotein MG307 homolog

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Macromolecule #65: Uncharacterized lipoprotein MG309 homolog

MacromoleculeName: Uncharacterized lipoprotein MG309 homolog / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 143.385078 KDa
SequenceString: CAQPTASTVE GLFKPSSAFA DRTDFSLSSI LQKSLINRES FNQYLAMRLA PVLRTFYEDN YDTDIKERLN GFTADTDNAF VSQEQNLRN QFRENYLVHL QTDIFDNTGG NQAAWKLRDV NNKIIDDFIS RIFAKNFVEY VQDGVGPLTK PTKSLIENTS N FKNIKLQP ...String:
CAQPTASTVE GLFKPSSAFA DRTDFSLSSI LQKSLINRES FNQYLAMRLA PVLRTFYEDN YDTDIKERLN GFTADTDNAF VSQEQNLRN QFRENYLVHL QTDIFDNTGG NQAAWKLRDV NNKIIDDFIS RIFAKNFVEY VQDGVGPLTK PTKSLIENTS N FKNIKLQP KFVNKNAKLK INNDAVYAAI QDKLLDQFIT NENPNLVSRV VFTNETPVDG FDNYFNTKVI QSPTPSYQFQ VF NKYNQQS GGTKGANGFN LLASNLKSYK NDQSKGIDIP NKFSSDSGGK LLLKASDMFD TFDPSFSAAF IQGYLALQKK SKG ADSKEV DSLIKDKSII ENFFVDNNTQ AAAAARAASS SSEGTIQLKT ASDGGGTTQS TVHKTDLVKI FGDKDVFAGE YKQQ IGNTN ANQTGGGGSG GGGGTSTGSS TGSSTETTTG NSSKAVVDLI EVKKDSSSQP DYILSRGKDG IHLMAVDGGS HYLTE SGRD VAKQKKFLLF RALQTKYGLV DTDTTYDFKL FDEVKKYFDT NRILFLFEAL LDLSSDTNNK DNFLSYPQFK KFADSI KSI EKDLKELVQA HYKQAVFNET AVAENKVTLK LAERNQPFID NERNNQIEQN GLAAKLPYEQ DAKTGHYNDL GNYYKDI ID NVDKKGTSTV KTTSSNTGQT KNFSEEVVSK LKDNKKKVEE AAKKHVEALK VFTIPSPLYS QVILVQTKLS FTPESTSL G LNLALNNYLT STELQNSIKL SYFQEDEAFK KIIDITNLTF SQQSGGTGGT NGNNNLTADN WKIFKETYLL DLFESQAQK SIFGHVGSSD KNSSTKTGIE GVLDTLYSSL NLEERLDSDD VIDYLSYLYT AHWLLKDNLK NYKQSLQSKL SRTSNAFLVW SVDSEKNKN DSQSTLSSTA SSTSNTGLIQ LRSVVSLAQN QAAGQGGDNN SDITQTEVKN PNFVFGSSVY DWTNSKTPEV N RAADDTSS FFYTKSSSSS TGAAQSSATV LRSLNQASGM TTKTAKNRYG FRGIVTSSTS GSLPEAVSRR LFKQFVNQTE KG VKVGGQM LITTAKSGKA TLVLKQQADD AESTTNNAYK GALFSFGSMD NLKNIINGIQ TQTEFDALYN HLTSDLNIDV TGV DKNKTL TEQKTSLTSF VDSNFKQSTQ SAQRGDTSAR SARSATVQIK KTQEDNQNTN YKDVFSRFDG YIGDNKVEEK NYTS YQFLS DGGKYHATFV KQVNLDDVEK IGTDSLKQED SSKDKRLNLS LEEFLAAIAL EALDPNNQTQ AINALISGNK KGLVK VGDF RIFSSISAQW VRRF

UniProtKB: Uncharacterized lipoprotein MG309 homolog

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Macromolecule #66: Uncharacterized lipoprotein MG338 homolog

MacromoleculeName: Uncharacterized lipoprotein MG338 homolog / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 140.427859 KDa
SequenceString: CSALNTSNLF PRQNRSKQLI GFTENNIIKP EAVLKAALAE DNGTETILRV NFGEALKSWY QNNKDRNIAT RLTIFSENVE DEHDNLLDQ KQQAEPINWP IELQKEYDQW GGSESSWKAL KLYDRLIADF QSLIFSNIVA NVQLTDGSDQ FKPTTKDNLD S TSNKIKFV ...String:
CSALNTSNLF PRQNRSKQLI GFTENNIIKP EAVLKAALAE DNGTETILRV NFGEALKSWY QNNKDRNIAT RLTIFSENVE DEHDNLLDQ KQQAEPINWP IELQKEYDQW GGSESSWKAL KLYDRLIADF QSLIFSNIVA NVQLTDGSDQ FKPTTKDNLD S TSNKIKFV NSKPNDPNGE FFANLQAYLF AQWVVEENPL PLTQAFFAYQ APKDGLDSLY DQAAIGSALQ LGYAFPAFRE PN NGQSQGK TTFDPTPNSA QNFGDFIKAV FPEQKNGQTQ QSNTSSRTGL FDWQTKWNTN GAANKLLVTK SNLRGAFKGV GLA TAIIDQ YEYLVGGSKT SSLPEVKVDS NKSNQNPLDS FFMEGKDAVA IRSIVSRAKI AMTDQTPGFK VNPAFVKVKQ SQQN DTFYQ NQRKLSGGQS GDNNSQGKHH YLQDAVRLTS SQAMAAASTG ADSSSGTNVG GSSGGNSVLI PLPRSAALTH TQQQV QQTT STLQTPVYAR GDDGTYALAI DGGDYFLANN KRDFTKQADI LLYRYLQAKS NNFKENGVEF SLNLLESGSL FQTWAQ TGL TAKLYGALVA MMGSGQGTQV KGSVQGSSRA ASVSVQTTQQ NRQQSTDTQE SEVVKLAKSL LKSSADLAKP FTDNPTF KK ALTDIQSEYK DYLAAAGKLS EFKKDLGEVS GLQQAIIDRA DKYIQLEKQA QKSAIGLGQP LPYQRASDGS YPALEKFF I PEDSAADGKV KASESGSAAL VTLKTTDSQK STNTVKQPDI KPTRENNDKK LKQLTSDVET KASSLITKWG ATPQIGSQF SEIVSLKSKD NKPQTNMILA LLSDVGIKWT KILNSFKEWF FTNTNDFKNN YDSEKKELKG NEYKDFNDLV KQTLYLRSWQ RLTSKEKFG YYKELGSVKA QAAQSGMVSL SSSAAVANAV ASSGMQKSGD QTLLELGKKA FESELEASSS DGQYKYLRFL S TLMWLVKD GAKNYKRLLQ QAITVGTRAF VSWTVSYDDT ATASAAAAKA QVAVLKTAQA TNTQSDNPFN KFVQNPDYVQ GS ETNWFND KSTPIKPDSL LESESTYNFT AEPFDDKTKS QKRSTGGTTN EKHFFGFNGL TINSPQSVST ASAGLTEQIF NNF GQLVTS SDKSGALSQY KDKATLKRLI QNTNSDAELN AFGEVLHRAV NVDTSNLGRF NSSGEPLISF DNKKKFLVDV VDKL DDVYF NKFEGYVGQT KVKMSDSSSS SQGTKTIRKP KPHHSPRTRV SRLWAMSFRL PTRTLTKFLL VEKLIRTVL

UniProtKB: Uncharacterized lipoprotein MG338 homolog

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Macromolecule #67: Uncharacterized lipoprotein MG348 homolog

MacromoleculeName: Uncharacterized lipoprotein MG348 homolog / type: protein_or_peptide / ID: 67 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 30.410285 KDa
SequenceString: CSNIDKPNVF RTLSQQSVEN KVDYSKLPKE NKTVRNLVFG TAEYNDGNYV LVVTTETDSS QINFLNGSNN QAVSTENWAG DLGTTVKQV QNRYSTYPKG VKFLIWNDID PNPVKWNPFA RYPVIASDNE LAKQTDKDNS DKLRRNDESA IQYREIVTFI Q TVYSGSVN ...String:
CSNIDKPNVF RTLSQQSVEN KVDYSKLPKE NKTVRNLVFG TAEYNDGNYV LVVTTETDSS QINFLNGSNN QAVSTENWAG DLGTTVKQV QNRYSTYPKG VKFLIWNDID PNPVKWNPFA RYPVIASDNE LAKQTDKDNS DKLRRNDESA IQYREIVTFI Q TVYSGSVN NLINQSNVHA QTVGTDVTKA IVIAFRKNNL DQISAHFYNP DNSNGSNAPG SNQPNQDSGN NGSTTPAAPA AA AAKKSYS AGSFGVKRHA VQVSINFLNF LDSVYTPLN

UniProtKB: Uncharacterized lipoprotein MG348 homolog

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Macromolecule #8: 23S ribosomal RNA

MacromoleculeName: 23S ribosomal RNA / type: rna / ID: 8 / Number of copies: 1
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 940.9115 KDa
SequenceString: UACAAUAAGU UACUAAGGGC UUAUGGUGGA UGCCUUGGCA CUAAUAGGCG AUGAAGGACG UGUUAACCUG CGAUAAGCUU CGGGUAGGU GGUAAGAACC UCAGAUCCGG AGAUUUCCGA AUGGAGCAAU CCGGUAGUUG GAAACAGCUA UCAUUAAUUG A UGAAUAAA ...String:
UACAAUAAGU UACUAAGGGC UUAUGGUGGA UGCCUUGGCA CUAAUAGGCG AUGAAGGACG UGUUAACCUG CGAUAAGCUU CGGGUAGGU GGUAAGAACC UCAGAUCCGG AGAUUUCCGA AUGGAGCAAU CCGGUAGUUG GAAACAGCUA UCAUUAAUUG A UGAAUAAA UAGUCAAUUA AAGCAAUACG UGGUGAAGUG AAACAUCUCA GUAGCCACAG GAAAAGAAAA CGAAUGUGAU UC CGUGUGU AGUGGCGAGC GAAAGCGGAA CAGGCCAAAC UUAUCAUUAG AUAGGGGUUG UAGGGCUUGC AAUGUGGACU UGA AAACGA UAGAAGAAGC UGUUGGAAAG CAGCGCGCAA AAGGGUGAUA GCCCCGUAUU UGAAAUUGUU UUCAUACCUA GCGA GAUCC CUGAGUAGCU CGGAAAACGU UAUUUUGAGU GAAUCUGCCC AGACCAUUGG GUAAGCCUAA AUACUAAUUA GUGAC CGAU AGCGAAACAG UACCGUGAGG GAAAGGUGAA AAGAACCCAG AGAUGGGAGU GAAAUAGAUU CUGAAACCAU AUGCCU ACA ACGUGUCAGA GCACAUUAAU GUGUGAUGGC GUGCGUUUUG AAGUAUGAGC CGGCGAGUUA UGAUAGCAAG CGUUAGU UA ACCAGGAGAU GGGGAGCUGU AGCGAAAGCG AGUUUUAAAA GAGCGUUUGU UUGUUAUUAU AGACCCGAAA CGGGUUGA G CUAGUCAUGA GCAGGUUGAA GGUUGAGUAA CAUCAACUGG AGGACCGAAC CGACUCUCGU UGAAACGAUA GCGGAUGAC UUGUGAUUAG GGGUGAAAUU CCAAUCGAAA UCCGUGAUAG CUGGUUCUCG UCGAAAUAGC UUUAAGGCUA GCGUGAGAUC ACAAAUAAG UGGAGGUAAA GCUACUGAAU GUAUGAUGGC GCCACCUAGG CGUACUGAAU ACAAUUAAAC UCUGAAUGCC A UUUAUUUU AUUCUCGCAG UCAGACAGUG GGGGAUAAGC UUCAUUGUCA AGAGGGGAAG AGCCCAGAUC AUUAAAUAAG GU CCCCAAA AUAUACUAAG UGGAAAAGGA UGUGAAAGUG CUAAAACAGC AAGGAUGUUG GCUUAGAAGC AGCCAUCGUU UAA AGAGUG CGUAACAGCU CACUUGUCGA GUGUUUUUGC GCCGAAGAUG UAACGGGGCU AAGUAUAUUA CCGAAUUUAU GGAU AAGAU UUAUAUCUUG UGGUAGACGA GCGUUGUAUU GGAGUUGAAG UCAAAGCGUG AGCAUUGGUG GAUCCAAUAC AAGUG AGAA UGCCGGCAUG AGUAACGCUU GGGAGUGAGA AUCUCCCAAA CCGAUUGACU AAGGUUUCCU GGACCAGGGU CGUCCU UCC AGGGUUAGUC UGGACCUAAG CUGAGGCUGA AAAGCGUAGG CGAUGGACAA CAGGUUAAUA UUCCUGUACU UACAGUU AG ACUGAUGGAG UGACAAAGAA GGUUUUCCAC CCCCAUAAUU GGAUUUGGGG AUAAAUCAUA AGGUGGUACA AUAGGCAA A UCCGUUGUGC AUAACAUUGA GUGAUGAUGU CGAGUGAAUG AGUGAUCAAG UAGCGAAGGU GGUAUUAAUC AUGCUUUCA AGAAAAGCUU CUAGGGUUAA UCUAGCUGUA ACCAGUACCG AGAACGAACA CACGUAGUCA AGGAGAGGAU CCUAAGGUUA GCGAGUGAA CUAUAGCCAA GGAACUCUGC AAAUUAACCC CGUAAGUUAG CGAGAAGGGG UGCUUAUGUA AAAGUAAGCC G CAGUGAAG AACGAGGGGG GACUGUUUAA CUAAAACACA ACUCUAUGCC AAACCGUAAG GUGAUGUAUA UGGGGUGACA CC UGCCCAG UGCUGGAAGG UUAAAGAAGG AGGUUAGCGC AAGCGAAGCU UUUAACUGAA GCCCCAGUGA ACGGCGGCCG UAA CUAUAA CGGUCCUAAG GUAGCGAAAU UCCUAGUCGG GUAAAUUCCG UCCCGCUUGA AUGGUGUAAC CAUCUCUUGA CUGU CUCGG CUAUAGACUC GGUGAAAUCC AGGUACGGGU GAAGACACCC GUUAGGCGCA ACGGGACGGA AAGACCCCGU GAAGC UUUA CUGUAGCUUA AUAUUGAUCA GGACAUUAUC AUGUAGAGAA UAGGUAGGAG CAAUCGAUGC AAGUUCGCUA GGACUU GUU GAUGCGAAAG GUGGAAUACU ACCCUUGGUU GUGUGCUGUU CUAAUUGGUA ACUGUUAUCC AGUUUCAAGA CAGUGUU AG GUGGGCAGUU UGACUGGGGC GGUCGCCUCC UAAAAGGUAA CGGAGGCGUA CAAAGGUACC UUCAGUACGG UUGGAAAU C GUAUGUAGAG UGUAAUGGUG UAAGGGUGCU UGACUGUGAG ACAUACAGGU CGAACAGGUG AGAAAUCAGG UCAUAGUGA UCCGGUGGUC CAGUAUGGAA UGGCCAUCGC UCAACGGAUA AAAGCUACUC CGGGGAUAAC AGGCUGAUAC UGCCCAAGAG UUCAUAUCG ACGGCAGUGU UUGGCACCUC GAUGUCGACU CAUCUCAUCC UCGAGCUGAA GCAGGUUCGA AGGGUUCGGC U GUUCGCCG AUUAAAGAGA UACGUGAGUU GGGUUCAAAC CGUCGUGAGA CAGGUUGGUC CCUAUCUAUU GUGCCCGUAG GA AGAUUGA AGAGUGUUGC UUCUAGUACG AGAGGACCGA AGCGAGGACA CCUCUUAUGC UCCAGUUGUA GCGCCAGCUG CAC CGCUGG GUAGUAACGU GUCUAUUAGA UAAACGCUGA AAGCAUCUAA GUGUGAAACU AUCUCAAAGA UUAAUCUUCC CAUU UCGCA AGAAAGUAAG AGCCGUCAAA GACGAUGACG UUGAUAGGUU ACAGGUGUAA GCAUAGUGAU AUGUUGAGCU GAGUA AUAC UAAUUGCUCG AGGACUUAUU GGA

GENBANK: GENBANK: U00089.2

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Macromolecule #9: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 9 / Number of copies: 1
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 34.796676 KDa
SequenceString:
UUUGGUGCCC AUGUCGCUGU GGAAACACCU GGUUCCAUUU CGAACCCAGC AGUUAAGCAC AGUGGAGCCG AAUGUAGCUG UUUCAGUGA GAAUAGGAAA GCACCAAGC

GENBANK: GENBANK: CP179154.1

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Macromolecule #10: 16S ribosomal RNA

MacromoleculeName: 16S ribosomal RNA / type: rna / ID: 10 / Number of copies: 1
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 492.302406 KDa
SequenceString: UUUUUCUGAG AGUUUGAUCC UGGCUCAGGA UUAACGCUGG CGGCAUGCCU AAUACAUGCA AGUCGAUCGA AAGUAGUAAU ACUUUAGAG GCGAACGGGU GAGUAACACG UAUCCAAUCU ACCUUAUAAU GGGGGAUAAC UAGUUGAAAG ACUAGCUAAU A CCGCAUAA ...String:
UUUUUCUGAG AGUUUGAUCC UGGCUCAGGA UUAACGCUGG CGGCAUGCCU AAUACAUGCA AGUCGAUCGA AAGUAGUAAU ACUUUAGAG GCGAACGGGU GAGUAACACG UAUCCAAUCU ACCUUAUAAU GGGGGAUAAC UAGUUGAAAG ACUAGCUAAU A CCGCAUAA GAACUUUGGU UCGCAUGAAU CAAAGUUGAA AGGACCUGCA AGGGUUCGUU AUUUGAUGAG GGUGCGCCAU AU CAGCUAG UUGGUGGGGU AACGGCCUAC CAAGGCAAUG ACGUGUAGCU AUGCUGAGAA GUAGAAUAGC CACAAUGGGA CUG AGACAC GGCCCAUACU CCUACGGGAG GCAGCAGUAG GGAAUUUUUC ACAAUGAGCG AAAGCUUGAU GGAGCAAUGC CGCG UGAAC GAUGAAGGUC UUUAAGAUUG UAAAGUUCUU UUAUUUGGGA AGAAUGACUU UAGCAGGUAA UGGCUAGAGU UUGAC UGUA CCAUUUUGAA UAAGUGACGA CUAACUAUGU GCCAGCAGUC (G7M)GCGGUAAUA CAUAGGUCGC AAGCGUUAUC CG GAUUUAU UGGGCGUAAA GCAAGCGCAG GCGGAUUGAA AAGUCUGGUG UUAAAGGCAG CUGCUUAACA GUUGUAUGCA UUG GAAACU AUUAAUCUAG AGUGUGGUAG GGAGUUUUGG AAUUUCAUGU GGAGCGGUGA AAUGCGUAGA UAUAUGAAGG AACA CCAGU GGCGAAGGCG AAAACUUAGG CCAUUACUGA CGCUUAGGCU UGAAAGUGUG GGGAGCAAAU AGGAUUAGAU ACCCU AGUA GUCCACACCG UAAACGAUAG AUACUAGCUG UCGGGGCGAU CCCCUCGGUA GUGAAGUUAA CACAUUAAGU AUCUCG CCU GGGUAGUACA UUCGCAAGAA UGAAACUCAA ACGGAAUUGA CGGGGACCCG CACAAGUGGU GGAGCAUGUU GCUUAAU UC GACGGUACAC GAAAAACCUU ACCUAGACUU GACAUCCUUG GCAAAAUUAU GGAAACAUAA UGGAGGUUAA CCGAGUGA C AGGUGGUGCA UGGUUGUCGU CAGCUCGUGU CGUGAGAUGU UGGGUUAAGU CCCGCAACGA GCGCAACCCU UAUCGUUAG UUACAUUGUC UAGCGAGACU GCUAAUGCAA AUUGGAGGAA GGAAGGGAUG ACGUCAAAUC AUCAUGCCCC UUAUGUCUAG GGCUGCAAA CGUGCUACAA UGGCCAAUAC AAACAGUCGC CAGCUUGUAA AAGUGAGCAA AUCUGUAAAG UUGGUCUCAG U UCGGAUUG AGGGCUGCAA UUCGUCCUCA UGAAGUCGGA AUCACUAGUA AUCGCGAAUC AGCUAUGUCG CGGUGAAUAC GU UCUCGGG UCUUGUACAC ACCGCCCGUC AAACUAUGAA AGCUGGUAAU AUUUAAAAAC GUGUUGCUAA CCAUUAGGAA GCG CAUGUC AAGGAUAGCA CCGGUGAUUG GAGUUAAGUC GUAACAAGGU ACCCCUACGA G(MA6)(MA6)AACGUGG GGGUGG AUC ACCUCCUUUC UA

GENBANK: GENBANK: U00089.2

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Macromolecule #11: 16S ribosomal RNA

MacromoleculeName: 16S ribosomal RNA / type: rna / ID: 11 / Number of copies: 1
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 492.303406 KDa
SequenceString: UUUUUCUGAG AGUUUGAUCC UGGCUCAGGA UUAACGCUGG CGGCAUGCCU AAUACAUGCA AGUCGAUCGA AAGUAGUAAU ACUUUAGAG GCGAACGGGU GAGUAACACG UAUCCAAUCU ACCUUAUAAU GGGGGAUAAC UAGUUGAAAG ACUAGCUAAU A CCGCAUAA ...String:
UUUUUCUGAG AGUUUGAUCC UGGCUCAGGA UUAACGCUGG CGGCAUGCCU AAUACAUGCA AGUCGAUCGA AAGUAGUAAU ACUUUAGAG GCGAACGGGU GAGUAACACG UAUCCAAUCU ACCUUAUAAU GGGGGAUAAC UAGUUGAAAG ACUAGCUAAU A CCGCAUAA GAACUUUGGU UCGCAUGAAU CAAAGUUGAA AGGACCUGCA AGGGUUCGUU AUUUGAUGAG GGUGCGCCAU AU CAGCUAG UUGGUGGGGU AACGGCCUAC CAAGGCAAUG ACGUGUAGCU AUGCUGAGAA GUAGAAUAGC CACAAUGGGA CUG AGACAC GGCCCAUACU CCUACGGGAG GCAGCAGUAG GGAAUUUUUC ACAAUGAGCG AAAGCUUGAU GGAGCAAUGC CGCG UGAAC GAUGAAGGUC UUUAAGAUUG UAAAGUUCUU UUAUUUGGGA AGAAUGACUU UAGCAGGUAA UGGCUAGAGU UUGAC UGUA CCAUUUUGAA UAAGUGACGA CUAACUAUGU GCCAGCAGUC (7MG)GCGGUAAUA CAUAGGUCGC AAGCGUUAUC CG GAUUUAU UGGGCGUAAA GCAAGCGCAG GCGGAUUGAA AAGUCUGGUG UUAAAGGCAG CUGCUUAACA GUUGUAUGCA UUG GAAACU AUUAAUCUAG AGUGUGGUAG GGAGUUUUGG AAUUUCAUGU GGAGCGGUGA AAUGCGUAGA UAUAUGAAGG AACA CCAGU GGCGAAGGCG AAAACUUAGG CCAUUACUGA CGCUUAGGCU UGAAAGUGUG GGGAGCAAAU AGGAUUAGAU ACCCU AGUA GUCCACACCG UAAACGAUAG AUACUAGCUG UCGGGGCGAU CCCCUCGGUA GUGAAGUUAA CACAUUAAGU AUCUCG CCU GGGUAGUACA UUCGCAAGAA UGAAACUCAA ACGGAAUUGA CGGGGACCCG CACAAGUGGU GGAGCAUGUU GCUUAAU UC GACGGUACAC GAAAAACCUU ACCUAGACUU GACAUCCUUG GCAAAAUUAU GGAAACAUAA UGGAGGUUAA CCGAGUGA C AGGUGGUGCA UGGUUGUCGU CAGCUCGUGU CGUGAGAUGU UGGGUUAAGU CCCGCAACGA GCGCAACCCU UAUCGUUAG UUACAUUGUC UAGCGAGACU GCUAAUGCAA AUUGGAGGAA GGAAGGGAUG ACGUCAAAUC AUCAUGCCCC UUAUGUCUAG GGCUGCAAA CGUGCUACAA UGGCCAAUAC AAACAGUCGC CAGCUUGUAA AAGUGAGCAA AUCUGUAAAG UUGGUCUCAG U UCGGAUUG AGGGCUGCAA UUCGUCCUCA UGAAGUCGGA AUCACUAGUA AUCGCGAAUC AGCUAUGUCG CGGUGAAUAC GU UCUCGGG UCUUGUACAC ACCGCCCGUC AAACUAUGAA AGCUGGUAAU AUUUAAAAAC GUGUUGCUAA CCAUUAGGAA GCG CAUGUC AAGGAUAGCA CCGGUGAUUG GAGUUAAGUC GUAACAAGGU ACCCCUACGA G(MA6)(MA6)AACGUGG GGGUGG AUC ACCUCCUUUC UA

GENBANK: GENBANK: U00089.2

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Macromolecule #12: tRNA-Asp (P-site)

MacromoleculeName: tRNA-Asp (P-site) / type: rna / ID: 12 / Number of copies: 1
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 24.731953 KDa
SequenceString:
GCCCGGA(4SU)AG CUCAGUCGGU AGAGCAGGGG A(PSU)UGAA(MIA)A(PSU)C CCCGU(G7M)(3AU)CCU UGGU (PSU)CGAU UCCGAGUCCG GGCACCA

+
Macromolecule #13: tRNA-Lys (A-site)

MacromoleculeName: tRNA-Lys (A-site) / type: rna / ID: 13 / Number of copies: 1
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 24.697787 KDa
SequenceString:
GGGUCGUUAG CUCAGUUGGU AGAGCAGUUG ACU(U8U)UU(T6A)A(PSU)C AAUUGG(3AU)CGC AGG(5MU)(PSU)C GAA UCCUGCACGA CCCACCA

+
Macromolecule #17: mRNA

MacromoleculeName: mRNA / type: rna / ID: 17 / Number of copies: 1
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 6.61798 KDa
SequenceString:
CUGCCUUCAA AGUCUUCAAA A

+
Macromolecule #37: mRNA

MacromoleculeName: mRNA / type: rna / ID: 37 / Number of copies: 1
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Molecular weightTheoretical: 6.698029 KDa
SequenceString:
CUGCUGAUAA GGUCUUCAAA A

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Macromolecule #68: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 68 / Number of copies: 7 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #69: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 69 / Number of copies: 317 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #70: 1,4-DIAMINOBUTANE

MacromoleculeName: 1,4-DIAMINOBUTANE / type: ligand / ID: 70 / Number of copies: 12 / Formula: PUT
Molecular weightTheoretical: 88.151 Da
Chemical component information

ChemComp-PUT:
1,4-DIAMINOBUTANE

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Macromolecule #71: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 71 / Number of copies: 1 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #72: water

MacromoleculeName: water / type: ligand / ID: 72 / Number of copies: 19 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statecell

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Sample preparation

BufferpH: 7.4
Details: Modified Hayflick medium: 14.7g/l Difco PPLO(Becton Dickinson), 20% (v/v) Gibco horse serum (New Zealand origin), 100 mM HEPES-Na; pH 7.4, 1% (w/w) glucose, 0.002% (w/w) phenol red, 1000 U/ml penicillin G.
GridModel: Quantifoil R2/1 / Support film - Material: CARBON / Support film - topology: HOLEY
VitrificationCryogen name: ETHANE / Instrument: HOMEMADE PLUNGER
Details: Back-side blotting for 2-3 seconds before plunging using a manual plunger without an environmental control chamber..
DetailsMycoplasma pneumoniae M129 cells were grown on gold Quantifoil grids at 37 Celsius in modified Hayflick medium. Treatment with different antibiotics for 15-20 minutes before plunge freezing.

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Electron microscopy

MicroscopeTFS KRIOS
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Image recording#0 - Image recording ID: 1 / #0 - Film or detector model: GATAN K2 SUMMIT (4k x 4k) / #0 - Detector mode: COUNTING / #0 - Average electron dose: 3.2 e/Å2 / #1 - Image recording ID: 2 / #1 - Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / #1 - Average electron dose: 3.34 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.0 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Image recording ID2
DetailsData collected on both GATAN K2 and K3 camera were combined for averaging
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 9.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0.8) / Number subtomograms used: 2304
ExtractionNumber tomograms: 360 / Number images used: 77980 / Software: (Name: PyTom, Warp (ver. 1.0.9))
Details: Ribosome-Sec translocon-Dome supercomplex in chloramphenicol treated Mycoplasma pneumoniae cells by K2 camera: 18957 sub-tomograms from 65 tomograms Ribosome-Sec translocon-Dome supercomplex ...Details: Ribosome-Sec translocon-Dome supercomplex in chloramphenicol treated Mycoplasma pneumoniae cells by K2 camera: 18957 sub-tomograms from 65 tomograms Ribosome-Sec translocon-Dome supercomplex in puseudoridimycin treated Mycoplasma pneumoniae cells : 15314 sub-tomograms from 86 tomograms ribosome-cage supercomplexs in spectinomycin-treated Mycoplasma pneumoniae cells: 12935 sub-tomograms from 70 tomograms; ribosome-cage supercomplexs in chlooramphenicol-treated Mycoplasma pneumoniae cells by K3 camera: 30774 sub-tomograms from 139 tomograms
CTF correctionSoftware - Name: Warp (ver. 1.0.9) / Type: PHASE FLIPPING ONLY
Final 3D classificationSoftware - Name: RELION (ver. 3.0.8)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0.8)
FSC plot (resolution estimation)

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