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- EMDB-55027: Cryo-EM structure of horse spleen apoferritin using the cryoWrite... -

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Basic information

Entry
Database: EMDB / ID: EMD-55027
TitleCryo-EM structure of horse spleen apoferritin using the cryoWriter automated grid preparation system with two-time writing with spiral pattern
Map data
Sample
  • Complex: Horse spleen apoferritin
Keywordson-grid mixing / cryo-EM / cryoWriter / single-particle / automated grid preparation / apoF / horse spleen apoferritin / METAL TRANSPORT
Biological speciesEquus caballus (horse)
Methodsingle particle reconstruction / cryo EM / Resolution: 1.71 Å
AuthorsChinmaya KV / Kube M / Stahlberg H
Funding support Switzerland, European Union, 2 items
OrganizationGrant numberCountry
Swiss National Science Foundation200021_200628 Switzerland
European Research Council (ERC)101118656European Union
CitationJournal: To Be Published
Title: CryoWriter: A Robotic Solution for Improved Cryo-EM Grid Preparation
Authors: Chinmaya KV / Ekundayo B / Di Fabrizio M / Mohammed I / Radecke J / Stahlberg H / Kube M
History
DepositionSep 5, 2025-
Header (metadata) releaseFeb 4, 2026-
Map releaseFeb 4, 2026-
UpdateFeb 4, 2026-
Current statusFeb 4, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_55027.png

Downloads & links

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Map

FileDownload / File: emd_55027.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.41 Å/pix.
x 600 pix.
= 246.72 Å		0.41 Å/pix.
x 600 pix.
= 246.72 Å		0.41 Å/pix.
x 600 pix.
= 246.72 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.4112 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0607
Minimum - Maximum-0.28281817 - 0.39206526
Average (Standard dev.)0.000008713496 (±0.012576838)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions600600600
Spacing600600600
CellA=B=C: 246.71999 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_55027_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_55027_half_map_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_55027_half_map_2.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Horse spleen apoferritin

EntireName: Horse spleen apoferritin
Components
  • Complex: Horse spleen apoferritin

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Supramolecule #1: Horse spleen apoferritin

SupramoleculeName: Horse spleen apoferritin / type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Equus caballus (horse)
Molecular weightTheoretical: 483 kDa/nm

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration5 mg/mL
BufferpH: 7.5
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec. / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Chamber humidity: 70 %

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionAlgorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 1.71 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 760785
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final 3D classificationNumber classes: 1 / Software - Name: cryoSPARC
FSC plot (resolution estimation)

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