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- EMDB-5502: Dissecting the in vivo assembly of the 30S ribosomal subunit reve... -

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Entry
Database: EMDB / ID: EMD-5502
TitleDissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM
Map data
SampleImmature ribosomal small subunit from rimm gene deleted E.coli strain:
ribosome-prokaryote
KeywordsRibosome biogenesis / 30S subunit assembly / RimM
Biological speciesEscherichia coli (E. coli)
Methodsingle particle reconstruction / cryo EM / Resolution: 13.1 Å
AuthorsGuo Q / Goto S / Chen Y / Muto A / Himeno H / Deng H / Lei J / Gao N
CitationJournal: Nucleic Acids Res / Year: 2013
Title: Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM and general features of the assembly process.
Authors: Qiang Guo / Simon Goto / Yuling Chen / Boya Feng / Yanji Xu / Akira Muto / Hyouta Himeno / Haiteng Deng / Jianlin Lei / Ning Gao /
Abstract: Ribosome biogenesis is a tightly regulated, multi-stepped process. The assembly of ribosomal subunits is a central step of the complex biogenesis process, involving nearly 30 protein factors in vivo ...Ribosome biogenesis is a tightly regulated, multi-stepped process. The assembly of ribosomal subunits is a central step of the complex biogenesis process, involving nearly 30 protein factors in vivo in bacteria. Although the assembly process has been extensively studied in vitro for over 40 years, very limited information is known for the in vivo process and specific roles of assembly factors. Such an example is ribosome maturation factor M (RimM), a factor involved in the late-stage assembly of the 30S subunit. Here, we combined quantitative mass spectrometry and cryo-electron microscopy to characterize the in vivo 30S assembly intermediates isolated from mutant Escherichia coli strains with genes for assembly factors deleted. Our compositional and structural data show that the assembly of the 3'-domain of the 30S subunit is severely delayed in these intermediates, featured with highly underrepresented 3'-domain proteins and large conformational difference compared with the mature 30S subunit. Further analysis indicates that RimM functions not only to promote the assembly of a few 3'-domain proteins but also to stabilize the rRNA tertiary structure. More importantly, this study reveals intriguing similarities and dissimilarities between the in vitro and the in vivo assembly pathways, suggesting that they are in general similar but with subtle differences.
Validation ReportSummary, Full report, XML, About validation report
History
DepositionSep 28, 2012-
Header (metadata) releaseNov 28, 2012-
Map releaseJan 16, 2013-
UpdateMar 6, 2013-
Current statusMar 6, 2013Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: -2.8
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: -2.8
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-3j2a
  • Surface level: -2.8
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol

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Map

FileDownload / File: emd_5502.map.gz / Format: CCP4 / Size: 7.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.76 Å/pix.
x 125 pix.
= 345. Å
2.76 Å/pix.
x 125 pix.
= 345. Å
2.76 Å/pix.
x 125 pix.
= 345. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.76 Å
Density
Contour LevelBy AUTHOR: -2.8 / Movie #1: -2.8
Minimum - Maximum-6.63006067 - 4.176404
Average (Standard dev.)-3.90336227 (±0.56780559)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-62-62-62
Dimensions125125125
Spacing125125125
CellA=B=C: 345.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.762.762.76
M x/y/z125125125
origin x/y/z0.0000.0000.000
length x/y/z345.000345.000345.000
α/β/γ90.00090.00090.000
start NX/NY/NZ-5029166
NX/NY/NZ106122134
MAP C/R/S123
start NC/NR/NS-62-62-62
NC/NR/NS125125125
D min/max/mean-6.6304.176-3.903

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Supplemental data

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Sample components

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Entire Immature ribosomal small subunit from rimm gene deleted E.coli strain

EntireName: Immature ribosomal small subunit from rimm gene deleted E.coli strain
Number of components: 1
MassTheoretical: 800 kDa / Experimental: 800 kDa

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Component #1: ribosome-prokaryote, Small subunit from rimm gene deleted E.coli ...

Ribosome-prokaryoteName: Small subunit from rimm gene deleted E.coli strain / a.k.a: immature 30S / Prokaryote: SSU 30S / Recombinant expression: No
MassTheoretical: 800 kDa / Experimental: 800 kDa
SourceSpecies: Escherichia coli (E. coli)

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Experimental details

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Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionBuffer solution: 150mM NH4Cl,10mM Tris-HCL,10mM MgCl2 / pH: 7.5
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Method: Blot for 1 seconds before plunging

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Electron microscopy imaging

Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company
ImagingMicroscope: FEI TECNAI F20 / Date: Jan 1, 2012
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Electron dose: 20 e/Å2 / Illumination mode: FLOOD BEAM
LensMagnification: 80000 X (nominal) / Imaging mode: BRIGHT FIELD / Defocus: 1300 - 4000 nm
Specimen HolderModel: GATAN LIQUID NITROGEN
CameraDetector: GENERIC GATAN (4k x 4k)

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 30262
Details: This is a classification volume (No. 3) using ML3D methods.
Applied symmetry: C1 (asymmetric)
3D reconstructionAlgorithm: Reference projections / Software: Spider / CTF correction: Weiner filter / Resolution: 13.1 Å / Resolution method: FSC 0.5

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Atomic model buiding

Modeling #1Software: MDFF / Refinement protocol: flexible / Target criteria: Cross-correlation / Refinement space: REAL
Details: Protocol: Initial local fitting was done using Chimera and then MDFF was used for flexible fitting. ref: Trabuco, L.G., Villa, E., Mitra, K., Frank, J. and Schulten, K. (2008) Flexible ...Details: Protocol: Initial local fitting was done using Chimera and then MDFF was used for flexible fitting. ref: Trabuco, L.G., Villa, E., Mitra, K., Frank, J. and Schulten, K. (2008) Flexible fitting of atomic structures into electron microscopy maps using molecular dynamics. Structure, 16, 673-683
Input PDB model: 3OFA

3ofa
PDB Unreleased entry


Chain ID: A
Output model

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