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Yorodumi- EMDB-54774: Cryo-EM structure of the Arabidopsis thaliana 60S ribosomal subunit -
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Open data
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Basic information
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| Title | Cryo-EM structure of the Arabidopsis thaliana 60S ribosomal subunit | |||||||||
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Sample |
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Keywords | Arabidopsis / Ribosome / CryoEM | |||||||||
| Function / homology | Function and homology informationroot morphogenesis / adaxial/abaxial pattern specification / leaf morphogenesis / response to high light intensity / response to fungus / plant-type cell wall / developmental process / plasmodesma / chloroplast envelope / plant-type vacuole ...root morphogenesis / adaxial/abaxial pattern specification / leaf morphogenesis / response to high light intensity / response to fungus / plant-type cell wall / developmental process / plasmodesma / chloroplast envelope / plant-type vacuole / vacuole / response to UV-B / nucleocytoplasmic transport / plastid / protein-RNA complex assembly / maturation of LSU-rRNA / cytosolic ribosome / response to cold / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / chloroplast / modification-dependent protein catabolic process / protein tag activity / large ribosomal subunit / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly / response to oxidative stress / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / structural constituent of ribosome / protein ubiquitination / ribosome / translation / ribonucleoprotein complex / cell division / mRNA binding / ubiquitin protein ligase binding / nucleolus / endoplasmic reticulum / mitochondrion / RNA binding / extracellular region / zinc ion binding / nucleoplasm / nucleus / plasma membrane / cytoplasm / cytosol Similarity search - Function | |||||||||
| Biological species | ![]() ![]() | |||||||||
| Method | single particle reconstruction / cryo EM / Resolution: 2.9 Å | |||||||||
Authors | Karki S / Lu X / Paatero AO / Ruonala R / Tranter D / Guryanov S / Rehan S / Hellmann E / Haakonsson A / Butcher SJ ...Karki S / Lu X / Paatero AO / Ruonala R / Tranter D / Guryanov S / Rehan S / Hellmann E / Haakonsson A / Butcher SJ / Huiskonen JT / Kajander T / Helariutta Y / Paavilainen VO | |||||||||
| Funding support | United States, Finland, 2 items
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Citation | Journal: To Be PublishedTitle: Cryo-EM structure of the Arabidopsis thaliana ribosome in translating and non-translating states Authors: Karki S / Paavilainen V | |||||||||
| History |
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Structure visualization
| Supplemental images |
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Downloads & links
-EMDB archive
| Map data | emd_54774.map.gz | 302.3 MB | EMDB map data format | |
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| Header (meta data) | emd-54774-v30.xml emd-54774.xml | 62.2 KB 62.2 KB | Display Display | EMDB header |
| Images | emd_54774.png | 65.2 KB | ||
| Filedesc metadata | emd-54774.cif.gz | 13.7 KB | ||
| Others | emd_54774_half_map_1.map.gz emd_54774_half_map_2.map.gz | 323 MB 323 MB | ||
| Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-54774 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-54774 | HTTPS FTP |
-Related structure data
| Related structure data | ![]() 9scuMC ![]() 9sdpC M: atomic model generated by this map C: citing same article ( |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
| EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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| Related items in Molecule of the Month |
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Map
| File | Download / File: emd_54774.map.gz / Format: CCP4 / Size: 347.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
| Voxel size | X=Y=Z: 0.84 Å | ||||||||||||||||||||||||||||||||||||
| Density |
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| Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
| Details | EMDB XML:
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-Supplemental data
-Half map: #2
| File | emd_54774_half_map_1.map | ||||||||||||
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| Density Histograms |
-Half map: #1
| File | emd_54774_half_map_2.map | ||||||||||||
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| Density Histograms |
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Sample components
+Entire : 60S LSU Arabidopsis (root) ribosome complex
+Supramolecule #1: 60S LSU Arabidopsis (root) ribosome complex
+Macromolecule #1: 25S RNA
+Macromolecule #9: RNA (119-MER)
+Macromolecule #13: RNA (151-MER)
+Macromolecule #2: Large ribosomal subunit protein eL38z/eL38y
+Macromolecule #3: Large ribosomal subunit protein uL2x
+Macromolecule #4: Large ribosomal subunit protein eL42z/eL42y
+Macromolecule #5: (thale cress) hypothetical protein
+Macromolecule #6: Large ribosomal subunit protein eL39z/eL39x
+Macromolecule #7: Large ribosomal subunit protein uL24z
+Macromolecule #8: Large ribosomal subunit protein eL13z
+Macromolecule #10: Ubiquitin-ribosomal protein eL40z fusion protein
+Macromolecule #11: Large ribosomal subunit protein eL27x
+Macromolecule #12: Large ribosomal subunit protein eL14z
+Macromolecule #14: Large ribosomal subunit protein eL15y
+Macromolecule #15: Large ribosomal subunit protein uL15x
+Macromolecule #16: Large ribosomal subunit protein eL22z
+Macromolecule #17: 60S ribosomal protein L29
+Macromolecule #18: Large ribosomal subunit protein uL13w
+Macromolecule #19: Large ribosomal subunit protein uL3z
+Macromolecule #20: Large ribosomal subunit protein uL22z
+Macromolecule #21: Large ribosomal subunit protein eL30x
+Macromolecule #22: Large ribosomal subunit protein eL18y
+Macromolecule #23: Large ribosomal subunit protein uL4z
+Macromolecule #24: Large ribosomal subunit protein eL19z
+Macromolecule #25: Large ribosomal subunit protein eL31x
+Macromolecule #26: Ribosomal protein L18ae/LX family protein
+Macromolecule #27: Large ribosomal subunit protein uL18z
+Macromolecule #28: Large ribosomal subunit protein eL21x/eL21w
+Macromolecule #29: Large ribosomal subunit protein eL32z
+Macromolecule #30: Large ribosomal subunit protein uL14x/uL14z/uL14y
+Macromolecule #31: Large ribosomal subunit protein eL6x
+Macromolecule #32: Large ribosomal subunit protein eL24z
+Macromolecule #33: Large ribosomal subunit protein eL33z
+Macromolecule #34: Large ribosomal subunit protein uL23y
+Macromolecule #35: Large ribosomal subunit protein uL30y
+Macromolecule #36: Large ribosomal subunit protein eL34z
+Macromolecule #37: Large ribosomal subunit protein eL8z
+Macromolecule #38: Large ribosomal subunit protein uL29x
+Macromolecule #39: Large ribosomal subunit protein uL6z/uL6y
+Macromolecule #40: Large ribosomal subunit protein eL36y
+Macromolecule #41: Large ribosomal subunit protein uL16y
+Macromolecule #42: Large ribosomal subunit protein eL37z
+Macromolecule #43: Large ribosomal subunit protein uL5z
+Macromolecule #44: POTASSIUM ION
+Macromolecule #45: MAGNESIUM ION
+Macromolecule #46: ZINC ION
-Experimental details
-Structure determination
| Method | cryo EM |
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Processing | single particle reconstruction |
| Aggregation state | tissue |
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Sample preparation
| Buffer | pH: 7.4 Details: 50 mM Hepes, pH 7.4, 5 mM MgAc, 100 mM KAc, pH 7.5, 1 mM DTT, Complete protease inhibitor |
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| Grid | Model: Quantifoil / Material: COPPER |
| Vitrification | Cryogen name: ETHANE |
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Electron microscopy
| Microscope | TFS KRIOS |
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| Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 24.512 e/Å2 |
| Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
| Electron optics | Illumination mode: OTHER / Imaging mode: OTHER / Cs: 2.7 mm / Nominal defocus max: 1.2 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 105000 |
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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About Yorodumi



Keywords
Authors
United States,
Finland, 2 items
Citation











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Processing
FIELD EMISSION GUN
