[English] 日本語
Yorodumi- EMDB-54774: Cryo-EM structure of the Arabidopsis thaliana 60S ribosomal subunit -
+
Open data
-
Basic information
| Entry | ![]() | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Title | Cryo-EM structure of the Arabidopsis thaliana 60S ribosomal subunit | |||||||||
Map data | ||||||||||
Sample |
| |||||||||
Keywords | Arabidopsis / Ribosome / CryoEM | |||||||||
| Function / homology | Function and homology informationroot morphogenesis / response to fungus / adaxial/abaxial pattern specification / leaf morphogenesis / response to high light intensity / plant-type cell wall / developmental process / plasmodesma / chloroplast envelope / plant-type vacuole ...root morphogenesis / response to fungus / adaxial/abaxial pattern specification / leaf morphogenesis / response to high light intensity / plant-type cell wall / developmental process / plasmodesma / chloroplast envelope / plant-type vacuole / response to UV-B / vacuole / nucleocytoplasmic transport / plastid / response to cold / maturation of LSU-rRNA / protein-RNA complex assembly / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cytosolic ribosome / chloroplast / modification-dependent protein catabolic process / protein tag activity / large ribosomal subunit / ribosome biogenesis / response to oxidative stress / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / protein ubiquitination / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / cell division / mRNA binding / ubiquitin protein ligase binding / nucleolus / endoplasmic reticulum / mitochondrion / RNA binding / extracellular region / nucleoplasm / zinc ion binding / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | |||||||||
| Biological species | ![]() ![]() | |||||||||
| Method | single particle reconstruction / cryo EM / Resolution: 2.9 Å | |||||||||
Authors | Karki S / Lu X / Paatero AO / Ruonala R / Tranter D / Guryanov S / Rehan S / Hellmann E / Haakonsson A / Butcher SJ ...Karki S / Lu X / Paatero AO / Ruonala R / Tranter D / Guryanov S / Rehan S / Hellmann E / Haakonsson A / Butcher SJ / Huiskonen JT / Kajander T / Helariutta Y / Paavilainen VO | |||||||||
| Funding support | United States, Finland, 2 items
| |||||||||
Citation | Journal: Structure / Year: 2026Title: Cryo-EM structure of the Arabidopsisthaliana ribosome in translating and non-translating states. Authors: Sudeep Karki / Xun Lu / Anja O Paatero / Raili Ruonala / Dale Tranter / Sergey Guryanov / Shahid Rehan / Eva Hellmann / Anders Haakonsson / Sarah J Butcher / Juha T Huiskonen / Tommi ...Authors: Sudeep Karki / Xun Lu / Anja O Paatero / Raili Ruonala / Dale Tranter / Sergey Guryanov / Shahid Rehan / Eva Hellmann / Anders Haakonsson / Sarah J Butcher / Juha T Huiskonen / Tommi Kajander / Yrjö Helariutta / Ville O Paavilainen / ![]() Abstract: Translational control of mRNA expression underpins much of post-transcriptional gene expression driving developmental processes in eukaryotes. How ribosomal mRNA translation is executed in plants may ...Translational control of mRNA expression underpins much of post-transcriptional gene expression driving developmental processes in eukaryotes. How ribosomal mRNA translation is executed in plants may be especially important for adapting to an ever-changing environment. Arabidopsis thaliana is the main genetic model organism in plant science, yet structural insights into developmental processes where ribosomal gene expression plays important roles currently remain lacking. Here, we present cryoelectron microscopy (cryo-EM) structures of the Arabidopsis cytosolic ribosome in both translating and non-translating states, which together provide new structural details into how translation of cytosolic mRNAs is coordinated. Our structures reveal detailed information on the interactions of tRNAs, mRNA, and the nascent polypeptide with subunits of the actively translating 80S ribosome and the role of ribosomal RNA methylation in stabilizing ribosomal subunits. The structures provide a foundation for interpreting the functional effects of many mutations of ribosomal proteins affecting phenotypic effects for plant tissue development. | |||||||||
| History |
|
-
Structure visualization
| Supplemental images |
|---|
-
Downloads & links
-EMDB archive
| Map data | emd_54774.map.gz | 302.3 MB | EMDB map data format | |
|---|---|---|---|---|
| Header (meta data) | emd-54774-v30.xml emd-54774.xml | 65.6 KB 65.6 KB | Display Display | EMDB header |
| Images | emd_54774.png | 65.2 KB | ||
| Filedesc metadata | emd-54774.cif.gz | 14.2 KB | ||
| Others | emd_54774_half_map_1.map.gz emd_54774_half_map_2.map.gz | 323 MB 323 MB | ||
| Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-54774 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-54774 | HTTPS FTP |
-Related structure data
| Related structure data | ![]() 9scuMC ![]() 9sdpC ![]() 9sdqC M: atomic model generated by this map C: citing same article ( |
|---|---|
| Similar structure data | Similarity search - Function & homology F&H Search |
-
Links
| EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
|---|---|
| Related items in Molecule of the Month |
-
Map
| File | Download / File: emd_54774.map.gz / Format: CCP4 / Size: 347.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
| Voxel size | X=Y=Z: 0.84 Å | ||||||||||||||||||||||||||||||||||||
| Density |
| ||||||||||||||||||||||||||||||||||||
| Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
| Details | EMDB XML:
|
-Supplemental data
-Half map: #2
| File | emd_54774_half_map_1.map | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Projections & Slices |
| ||||||||||||
| Density Histograms |
-Half map: #1
| File | emd_54774_half_map_2.map | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Projections & Slices |
| ||||||||||||
| Density Histograms |
-
Sample components
+Entire : 60S LSU Arabidopsis (root) ribosome complex
+Supramolecule #1: 60S LSU Arabidopsis (root) ribosome complex
+Macromolecule #1: 25S RNA
+Macromolecule #9: RNA (119-MER)
+Macromolecule #13: RNA (151-MER)
+Macromolecule #2: Large ribosomal subunit protein eL38z/eL38y
+Macromolecule #3: Large ribosomal subunit protein uL2x
+Macromolecule #4: Large ribosomal subunit protein eL42z/eL42y
+Macromolecule #5: (thale cress) hypothetical protein
+Macromolecule #6: Large ribosomal subunit protein eL39z/eL39x
+Macromolecule #7: Large ribosomal subunit protein uL24z
+Macromolecule #8: Large ribosomal subunit protein eL13z
+Macromolecule #10: Ubiquitin-ribosomal protein eL40z fusion protein
+Macromolecule #11: Large ribosomal subunit protein eL27x
+Macromolecule #12: Large ribosomal subunit protein eL14z
+Macromolecule #14: Large ribosomal subunit protein eL15y
+Macromolecule #15: Large ribosomal subunit protein uL15x
+Macromolecule #16: Large ribosomal subunit protein eL22z
+Macromolecule #17: 60S ribosomal protein L29
+Macromolecule #18: Large ribosomal subunit protein uL13w
+Macromolecule #19: Large ribosomal subunit protein uL3z
+Macromolecule #20: Large ribosomal subunit protein uL22z
+Macromolecule #21: Large ribosomal subunit protein eL30x
+Macromolecule #22: Large ribosomal subunit protein eL18y
+Macromolecule #23: Large ribosomal subunit protein uL4z
+Macromolecule #24: Large ribosomal subunit protein eL19z
+Macromolecule #25: Large ribosomal subunit protein eL31x
+Macromolecule #26: Ribosomal protein L18ae/LX family protein
+Macromolecule #27: Large ribosomal subunit protein uL18z
+Macromolecule #28: Large ribosomal subunit protein eL21x/eL21w
+Macromolecule #29: Large ribosomal subunit protein eL32z
+Macromolecule #30: Large ribosomal subunit protein uL14x/uL14z/uL14y
+Macromolecule #31: Large ribosomal subunit protein eL6x
+Macromolecule #32: Large ribosomal subunit protein eL24z
+Macromolecule #33: Large ribosomal subunit protein eL33z
+Macromolecule #34: Large ribosomal subunit protein uL23y
+Macromolecule #35: Large ribosomal subunit protein uL30y
+Macromolecule #36: Large ribosomal subunit protein eL34z
+Macromolecule #37: Large ribosomal subunit protein eL8z
+Macromolecule #38: Large ribosomal subunit protein uL29x
+Macromolecule #39: Large ribosomal subunit protein uL6z/uL6y
+Macromolecule #40: Large ribosomal subunit protein eL36y
+Macromolecule #41: Large ribosomal subunit protein uL16y
+Macromolecule #42: Large ribosomal subunit protein eL37z
+Macromolecule #43: Large ribosomal subunit protein uL5z
+Macromolecule #44: POTASSIUM ION
+Macromolecule #45: MAGNESIUM ION
+Macromolecule #46: ZINC ION
-Experimental details
-Structure determination
| Method | cryo EM |
|---|---|
Processing | single particle reconstruction |
| Aggregation state | tissue |
-
Sample preparation
| Buffer | pH: 7.4 Details: 50 mM Hepes, pH 7.4, 5 mM MgAc, 100 mM KAc, pH 7.5, 1 mM DTT, Complete protease inhibitor |
|---|---|
| Grid | Model: Quantifoil / Material: COPPER |
| Vitrification | Cryogen name: ETHANE |
-
Electron microscopy
| Microscope | TFS KRIOS |
|---|---|
| Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 24.512 e/Å2 |
| Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
| Electron optics | Illumination mode: OTHER / Imaging mode: OTHER / Cs: 2.7 mm / Nominal defocus max: 1.2 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 105000 |
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
Movie
Controller
About Yorodumi



Keywords
Authors
United States,
Finland, 2 items
Citation














Z (Sec.)
Y (Row.)
X (Col.)




































Processing
FIELD EMISSION GUN
