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- EMDB-54774: Cryo-EM structure of the Arabidopsis thaliana 60S ribosomal subunit -

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Basic information

Entry
Database: EMDB / ID: EMD-54774
TitleCryo-EM structure of the Arabidopsis thaliana 60S ribosomal subunit
Map data
Sample
  • Complex: 60S LSU Arabidopsis (root) ribosome complex
    • RNA: x 3 types
    • Protein or peptide: x 40 types
  • Ligand: x 3 types
KeywordsArabidopsis / Ribosome / CryoEM
Function / homology
Function and homology information


root morphogenesis / adaxial/abaxial pattern specification / leaf morphogenesis / response to high light intensity / response to fungus / plant-type cell wall / developmental process / plasmodesma / chloroplast envelope / plant-type vacuole ...root morphogenesis / adaxial/abaxial pattern specification / leaf morphogenesis / response to high light intensity / response to fungus / plant-type cell wall / developmental process / plasmodesma / chloroplast envelope / plant-type vacuole / vacuole / response to UV-B / nucleocytoplasmic transport / plastid / protein-RNA complex assembly / maturation of LSU-rRNA / cytosolic ribosome / response to cold / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / chloroplast / modification-dependent protein catabolic process / protein tag activity / large ribosomal subunit / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly / response to oxidative stress / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / structural constituent of ribosome / protein ubiquitination / ribosome / translation / ribonucleoprotein complex / cell division / mRNA binding / ubiquitin protein ligase binding / nucleolus / endoplasmic reticulum / mitochondrion / RNA binding / extracellular region / zinc ion binding / nucleoplasm / nucleus / plasma membrane / cytoplasm / cytosol
Similarity search - Function
Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ribosomal protein L30e / : / Ribosomal protein L23 / Ribosomal protein L2, archaeal-type / metallochaperone-like domain / TRASH domain / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. ...Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ribosomal protein L30e / : / Ribosomal protein L23 / Ribosomal protein L2, archaeal-type / metallochaperone-like domain / TRASH domain / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L19, eukaryotic / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L44e signature. / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L10e / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / : / Ribosomal L40e family / Ribosomal protein L36e signature. / Ribosomal protein L30e signature 1. / Ribosomal protein L44e / Ribosomal protein L44 / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L35Ae, conserved site / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L35Ae signature. / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / : / Ribosomal Protein L6, KOW domain / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / 60S ribosomal protein L35 / Ribosomal protein L30e signature 2. / Ribosomal protein L7A/L8 / Ribosomal protein L30e, conserved site / : / Ribosomal protein L34Ae / Ribosomal protein L34e / 60S ribosomal protein L19 / Ribosomal protein L6e / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L7, eukaryotic / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / 60S ribosomal protein L6E / : / Ribosomal protein L30/YlxQ / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L18e / Ribosomal protein L14e domain / Ribosomal protein L31e, conserved site / Ribosomal protein L14 / Ribosomal protein L31e signature. / : / : / Ribosomal protein L19e, C-terminal domain / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e, N-terminal domain / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L35A superfamily / Ribosomal protein L32e, conserved site / Ribosomal protein L32e signature.
Similarity search - Domain/homology
Ribosomal protein L18ae/LX family protein / (thale cress) hypothetical protein / Ubiquitin-ribosomal protein eL40z fusion protein / 60S ribosomal protein L29 / Large ribosomal subunit protein eL38z/eL38y / Large ribosomal subunit protein eL42z/eL42y / Large ribosomal subunit protein uL3z / Large ribosomal subunit protein eL13z / Large ribosomal subunit protein eL18y / Large ribosomal subunit protein uL5z ...Ribosomal protein L18ae/LX family protein / (thale cress) hypothetical protein / Ubiquitin-ribosomal protein eL40z fusion protein / 60S ribosomal protein L29 / Large ribosomal subunit protein eL38z/eL38y / Large ribosomal subunit protein eL42z/eL42y / Large ribosomal subunit protein uL3z / Large ribosomal subunit protein eL13z / Large ribosomal subunit protein eL18y / Large ribosomal subunit protein uL5z / Large ribosomal subunit protein uL6z/uL6y / Large ribosomal subunit protein eL32z / Large ribosomal subunit protein uL15x / Large ribosomal subunit protein uL14x/uL14z/uL14y / Large ribosomal subunit protein eL8z / Large ribosomal subunit protein uL24z / Large ribosomal subunit protein eL27x / Large ribosomal subunit protein eL31x / Large ribosomal subunit protein eL39z/eL39x / Large ribosomal subunit protein uL30y / Large ribosomal subunit protein uL16y / Large ribosomal subunit protein uL2x / Large ribosomal subunit protein eL24z / Large ribosomal subunit protein eL34z / Large ribosomal subunit protein uL18z / Large ribosomal subunit protein eL37z / Large ribosomal subunit protein eL15y / Large ribosomal subunit protein uL22z / Large ribosomal subunit protein eL6x / Large ribosomal subunit protein eL21x/eL21w / Large ribosomal subunit protein uL13w / Large ribosomal subunit protein eL33z / Large ribosomal subunit protein eL30x / Large ribosomal subunit protein eL19z / Large ribosomal subunit protein eL36y / Large ribosomal subunit protein uL23y / Large ribosomal subunit protein uL29x / Large ribosomal subunit protein eL22z / Large ribosomal subunit protein uL4z / Large ribosomal subunit protein eL14z
Similarity search - Component
Biological speciesArabidopsis (plant) / Arabidopsis thaliana (thale cress)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsKarki S / Lu X / Paatero AO / Ruonala R / Tranter D / Guryanov S / Rehan S / Hellmann E / Haakonsson A / Butcher SJ ...Karki S / Lu X / Paatero AO / Ruonala R / Tranter D / Guryanov S / Rehan S / Hellmann E / Haakonsson A / Butcher SJ / Huiskonen JT / Kajander T / Helariutta Y / Paavilainen VO
Funding support United States, Finland, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)1R01GM132649[PVO1] United States
Academy of Finland338836 and 314672 Finland
CitationJournal: To Be Published
Title: Cryo-EM structure of the Arabidopsis thaliana ribosome in translating and non-translating states
Authors: Karki S / Paavilainen V
History
DepositionAug 12, 2025-
Header (metadata) releaseJun 10, 2026-
Map releaseJun 10, 2026-
UpdateJun 10, 2026-
Current statusJun 10, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

Downloads & links

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Map

FileDownload / File: emd_54774.map.gz / Format: CCP4 / Size: 347.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.84 Å/pix.
x 450 pix.
= 378. Å
0.84 Å/pix.
x 450 pix.
= 378. Å
0.84 Å/pix.
x 450 pix.
= 378. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.84 Å
Density
Contour LevelBy AUTHOR: 0.07
Minimum - Maximum-0.18871398 - 0.4814583
Average (Standard dev.)0.0057333643 (±0.027870022)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions450450450
Spacing450450450
CellA=B=C: 378.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_54774_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Half map: #1

Fileemd_54774_half_map_2.map
Projections & Slices
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Slices (1/2)
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Sample components

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Entire : 60S LSU Arabidopsis (root) ribosome complex

EntireName: 60S LSU Arabidopsis (root) ribosome complex
Components
  • Complex: 60S LSU Arabidopsis (root) ribosome complex
    • RNA: 25S RNA
    • RNA: RNA (119-MER)
    • RNA: RNA (151-MER)
    • Protein or peptide: Large ribosomal subunit protein uL2x
    • Protein or peptide: Large ribosomal subunit protein uL3z
    • Protein or peptide: Large ribosomal subunit protein uL4z
    • Protein or peptide: Large ribosomal subunit protein uL18z
    • Protein or peptide: Large ribosomal subunit protein eL6x
    • Protein or peptide: Large ribosomal subunit protein uL30y
    • Protein or peptide: Large ribosomal subunit protein eL8z
    • Protein or peptide: Large ribosomal subunit protein uL6z/uL6y
    • Protein or peptide: Large ribosomal subunit protein uL16y
    • Protein or peptide: Large ribosomal subunit protein uL5z
    • Protein or peptide: Large ribosomal subunit protein eL42z/eL42y
    • Protein or peptide: Large ribosomal subunit protein eL13z
    • Protein or peptide: Large ribosomal subunit protein eL14z
    • Protein or peptide: Large ribosomal subunit protein eL15y
    • Protein or peptide: Large ribosomal subunit protein uL13w
    • Protein or peptide: Large ribosomal subunit protein uL22z
    • Protein or peptide: Large ribosomal subunit protein eL18y
    • Protein or peptide: Large ribosomal subunit protein eL19z
    • Protein or peptide: Ribosomal protein L18ae/LX family protein
    • Protein or peptide: Large ribosomal subunit protein eL21x/eL21w
    • Protein or peptide: Large ribosomal subunit protein uL14x/uL14z/uL14y
    • Protein or peptide: Large ribosomal subunit protein eL24z
    • Protein or peptide: Large ribosomal subunit protein uL23y
    • Protein or peptide: Large ribosomal subunit protein uL24z
    • Protein or peptide: Large ribosomal subunit protein eL27x
    • Protein or peptide: Large ribosomal subunit protein uL15x
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: Large ribosomal subunit protein eL30x
    • Protein or peptide: Large ribosomal subunit protein eL31x
    • Protein or peptide: Large ribosomal subunit protein eL32z
    • Protein or peptide: Large ribosomal subunit protein eL33z
    • Protein or peptide: Large ribosomal subunit protein eL34z
    • Protein or peptide: Large ribosomal subunit protein uL29x
    • Protein or peptide: Large ribosomal subunit protein eL36y
    • Protein or peptide: Large ribosomal subunit protein eL37z
    • Protein or peptide: Large ribosomal subunit protein eL38z/eL38y
    • Protein or peptide: Large ribosomal subunit protein eL39z/eL39x
    • Protein or peptide: Ubiquitin-ribosomal protein eL40z fusion protein
    • Protein or peptide: (thale cress) hypothetical protein
    • Protein or peptide: Large ribosomal subunit protein eL22z
  • Ligand: POTASSIUM ION
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: 60S LSU Arabidopsis (root) ribosome complex

SupramoleculeName: 60S LSU Arabidopsis (root) ribosome complex / type: complex / ID: 1 / Parent: 0
Macromolecule list: #1, #9, #13, #3, #19, #23, #27, #31, #35, #37, #39, #41, #43, #4, #8, #12, #14, #18, #20, #22, #24, #26, #28, #30, #32, #34, #7, #11, #15, #17, #21, #25, #29, #33, #36, #38, #40, ...Macromolecule list: #1, #9, #13, #3, #19, #23, #27, #31, #35, #37, #39, #41, #43, #4, #8, #12, #14, #18, #20, #22, #24, #26, #28, #30, #32, #34, #7, #11, #15, #17, #21, #25, #29, #33, #36, #38, #40, #42, #2, #6, #10, #5, #16
Source (natural)Organism: Arabidopsis (plant)
Molecular weightTheoretical: 4.3 MDa

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Macromolecule #1: 25S RNA

MacromoleculeName: 25S RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 1.0947562499999999 MDa
SequenceString: GCGACCCCAG GUCAGGCGGG AUUACCCGCU GAGUUUAAGC AUA(OMU)CAA(OMU)AA GCGGAGGAAA AGAAACUAAC AA GGAUUCC CUUAGUAACG GCGAGCGAAC CGGGAAGAGC CCAGCUUGAA AAUCGGACGU CUUCGGCGUU CGAA(OMU)UGUA GUCUGGAGAA ...String:
GCGACCCCAG GUCAGGCGGG AUUACCCGCU GAGUUUAAGC AUA(OMU)CAA(OMU)AA GCGGAGGAAA AGAAACUAAC AA GGAUUCC CUUAGUAACG GCGAGCGAAC CGGGAAGAGC CCAGCUUGAA AAUCGGACGU CUUCGGCGUU CGAA(OMU)UGUA GUCUGGAGAA GCGUCCUCAG CGACGGACCG GGCCUAAGUU CCCUGGAAAG GGGCGCCAGA GAGGGUGAGA GCCCCGUCGU GCCCGGACC CUGUCGCACC ACGAGGCGCU GUCUACGAGU CGGGUUGUUU GGGAAUGCAG CCCCAAUCGG GCGGUAAAUU C CGUCCAAG GCUAAAUACG GGCGAGAGAC CGAUAGCGAA CAAGUACCGC GAGGUAAAGA UGAAAAGGAC UUUGAAAAGA GA GUCAAAG AGUGCUUGAA AUUGUCGGGA GGGAAGCGGA UGGGGGCCGG CGAUGCGUCC UGGUCGGAUG CGGAACGGAG CAA UCCGGU CCGCCGAUCG AUUCGGGGCG UGGACCGACG CGGAUUACGG UGGCGGCCUA AGCCCGGGCU UUUGAUACGC UUGU GGACG UCGCUGCCGU GAUCGUGGUC UGCAGCACGC GCCUAACGGC GUGCCUCGGC AUCAGCGUGC UCCGGGCGUC GGCCU GUGG GCUCCCCAUU CGACCCGUCU UG(1MA)AAC(A2M)CGG ACCAAGGAGU (OMC)(OMU)GACAUGUG UGCGAGUCAA CGGGUGAGU AAACCCGUAA GGCGCAAGGA AGCUGAUUGG CGGGAUCCCU CGCGGGUGCA CCGCCGACCG ACCUUGAUCU U CUGAGAAG GGUUCGAGUG UGAGCAUGCC (OMU)GUCGGGACC C(OMG)AAAGAUGG UGAACUAUGC CUGAGCGGGG UAAA GCCAG AGGAAACUCU GGUGGAAGCC CGCAGCGAUA CUGACGUGCA AAUCGUUCGU CUGACUUGGG UAUAG(OMG)GGCG A AAGACUAA UCGAACCAUC UAGU(A2M)GCUGG UUCCCUCCGA AGUUUCCCUC AGGAUAGCUG GAGCUCGGCG AGUUCUAU C GGGUAAAGCC AAUGAUUAGA GGCAUUGGGG GCGCAACGCC CUCGACCUAU UCUCAAACUU UAAA(OMU)AGGUA GGACG UGUC GGCUGCUUUG UUGAGCCGUC ACACGGAAUC GAGAGCUCCA AGUGGGCCAU UUUUGGUAAG C(A2M)GAACUGGC GA UGCGGGA UGAACCGGAA GCCGGGUUAC GGUGCCCAAC UGCGCGCUAA CCUAGAACCC ACAAAGGGUG UUGGUCGAUU AAG ACAGCA GGACGGUGGU CAUGGAAGUC GAAAUCCGCU AAGGAGUGUG UAACAACUCA CCUGCCGAAU CAACUAGCCC CGAA AAUGG AUGGCGCUUA AGCGCGCGAC CUAUACCCGG CCGUCGGGGC AAGAGCCAGG CCUCGAUG(A2M)G UAGGAGGGCG C GGCGGUCG CUGCAAAACC UAGGGCGCGA GCCCGCGCGG AGCGGCCGUC GGUGCAGAU(OMC) UUGGUGGUAG U(A2M) (OMG)CAAAUA UUCAAAUGAG AACUUUGAAG GCCGAAGAGG GGAAAGGUUC CAUGUGAACG GCACUUGCAC AUGGGUUAG UCGAUCCUAA GAGUCGGGGG AAACCCGUCU GAUAGCGCUU AAGCGCGAAC UUCGAAAGGG GAUCCGGUUA AAAUUCCGGA ACCGGGACG UGGCGGUUGA CGGCAACGUU AGGGAGUCCG GAGACGUCGG CGGGGGCCUC GGGAAGAGUU AUCUUUUCUG U UUAACAGC CUGCCCACCC UGGAAACGGC UCAGCCGGAG GUAGGGUCCA GCGGCUGGAA GAGCACCGCA CGUCGCGUGG UG UCCGGUG CGCCCCCGGC GGCCCUUGAA AAUCCGGAGG ACCGAGUGCC GCUCACGCCC GGUCGUACUC AUAA(OMC)CGCA UCA(OMG)GUCU(OMC)C AAGGUGAACA GCCUCUGGUC GAUGGAACAA (OMU)GUAGGCAAG GGAAGUCGGC AAAAUGGA U CCGUAACUUC GGGAAAAGGA UUGGCUCUGA GGGCUGGGCU CGGGGGUCCC AGUUCCGAAC CCGUCGGCUG UCAGCGGAC UGCUCGAGCU GCUUCCGCGG CGAGAGCGGG UCGCCGCGUG CCGGCCGGGG GACGGACUGG GAACGGCUCU CUCGGGAGCU UUCCCCGGG CGUCGAACAG UCAGCUCAGA ACUGGUACGG ACAAGG(OMG)G(A2M)A UCCGACUGUU UAAUUAAAAC AAA GCAUUG CGAUGGUCCC UGCGGAUGCU AACGCAAUGU GAUUUCUGCC (OMC)AGUGCUCUG AAUGUCAAAG UGA(A2M)GA AAU UCAACCAAGC GCGGGUAAAC GGCGGGAGUA ACUAUGACUC UCUUAAGGUA GC(5MC)AAAUGCC UC(OMG)UCAU (OMC)U AAUUAGUGAC GCGCAUGAAU GGAUUA(A2M)CGA GAUUCCCACU GU(OMC)CCUGUCU ACUAUCCAGC GAAACC ACA G(OMC)CAAGGGAA CGGGCUUGGC AGAAUCA(OMG)CG G(OMG)GAAAGAAG ACCCU(OMG)UUGA GCUUGAC (OMU)C UAGUCCGACU UUGUGAAAUG ACUUGAGAGG UGUAGGAUAA GUGGGAGCUU CGGCGCAAGU GAAAUACCAC UACU UUUAA CGUUAUUUUA CUUACUCCGU GAAUCGGAGG CGGGGUACAA CCCCUGUUUU UGGUCCCAAG GCUCGCUUCG GCGGG UCGA UCCGGGCGGA GGACAUUGUC AGGUGGGGAG UUUGGCUG(OMG)G GCGGCACAUC UGUUAAAAG(A2M) UAACGCAG G (OMU)GUCCUAAGA UGAGCUCAAC GAGAACAGAA AU(OMC)UCGUGUG GAACAAAAGG GUAAAAGCUC GUUUGAUUCU G AUUUUCAG UACGAA(OMU)ACG AACCGUGAAA GCGUGGCCUA UCGAUCCUUU AGACCUUCGG AAUUUGAAGC UA(OMG)A GGUGU CAGAAAAGUU ACCACAG(OMG)GA UAACUGGCUU GUGGCAGCCA AGCGUUCAUA GCGACGUUGC UUUUUGAUCC U U(5MC)GAUGUC GG(OMC)UCU(OMU)CCU AUCAUUGUGA AGCAGAAUUC ACCAAGUGUU (OMG)GAUU(OMG)UUCA CC CACCAAU AGGGAACGUG (A2M)G(OMC)UGGG(UR3)UU AGACCGUCGU GAGACAGGUU AGUUUUACCC UACUGAUGCC C GCGUCGCG AUAGUAAUUC AACCUAGUAC GAGAGGAACC GUUGAUUCGC ACAAUUGGUC AUCGCGCUUG GUUGAAAAGC CA GUGGCGC GAAGCUACCG UGCGCUGGAU UAUGACUGAA CGCCUCUAAG UCAGAAUCCG GGCUAGAAGC GACGCAUGCG CCC GCCGCC CGAUUGCCGA CCCUCAGUAG GAGCUUAGGC UCCAAAGGCA CGUGUCGUUG GCUAAGUCCG UUCGGCGGAA GCGU CGUUC GGACCGCCUU GAAUUAUAAU UACCACCGAG CGGCGGGUAG AAUCCUUUGC AGACGAC(OMU)UA AAUACGCGAC G GGGUAUUG UAAGUGGCAG AGUGGCCUUG CUGCCACGAU CCACUGAGAU UCAGCCCUUU GUCGCUAAGA UUCGA

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Macromolecule #9: RNA (119-MER)

MacromoleculeName: RNA (119-MER) / type: rna / ID: 9 / Number of copies: 1
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 38.917082 KDa
SequenceString:
GGAUGCGAUC AUACCAGCAC UAAUGCACCG GAUCCCAUCA GAACUCCGCA GUUAAGCGUG CUUGGGCGAG AGUAGUACUA GGAUGGGUG ACCUCCCGGG AAGUCCUCGU GUUGCAUCCC UC

GENBANK: GENBANK: OX291454.1

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Macromolecule #13: RNA (151-MER)

MacromoleculeName: RNA (151-MER) / type: rna / ID: 13 / Number of copies: 1
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 52.785336 KDa
SequenceString:
AAAACGACUC UCGGCAACGG AUAUCUCGGC UCUCGCAUCG AUGAAGAACG UAGCGAAAUG CGAUACUUGG UGUGAAUUGC AGAAUCCCG UGAACCAUCG AGUCUUUGAA CGCAAGUUGC GCCCCAAGCC UUCUGGCCGA GGGCACGUCU GCCUGGGUGU C ACAA

GENBANK: GENBANK: OZ281780.1

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Macromolecule #2: Large ribosomal subunit protein eL38z/eL38y

MacromoleculeName: Large ribosomal subunit protein eL38z/eL38y / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 8.139694 KDa
SequenceString:
MPKQIHEIKD FLLTARRKDA RSVKIKRSKD IVKFKVRCSR YLYTLCVFDQ EKADKLKQSL PPGLSVQDL

UniProtKB: Large ribosomal subunit protein eL38z/eL38y

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Macromolecule #3: Large ribosomal subunit protein uL2x

MacromoleculeName: Large ribosomal subunit protein uL2x / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 28.008529 KDa
SequenceString: MGRVIRAQRK GAGSVFKSHT HHRKGPAKFR SLDFGERNGY LKGVVTEIIH DPGRGAPLAR VAFRHPFRFK KQKELFVAAE GMYTGQFLY CGKKATLVVG NVLPLRSIPE GAVICNVEHH VGDRGVFARA SGDYAIVIAH NPDNDTSRIK LPSGSKKIVP S GCRAMIGQ ...String:
MGRVIRAQRK GAGSVFKSHT HHRKGPAKFR SLDFGERNGY LKGVVTEIIH DPGRGAPLAR VAFRHPFRFK KQKELFVAAE GMYTGQFLY CGKKATLVVG NVLPLRSIPE GAVICNVEHH VGDRGVFARA SGDYAIVIAH NPDNDTSRIK LPSGSKKIVP S GCRAMIGQ VAGGGRTEKP MLKAGNAYHK YRVKRNCWPK VRGVAMNPVE HPHGGGNHQH IGHASTVRRD APPGKKVGLI AA RRTGRLR GQAAALASKA D

UniProtKB: Large ribosomal subunit protein uL2x

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Macromolecule #4: Large ribosomal subunit protein eL42z/eL42y

MacromoleculeName: Large ribosomal subunit protein eL42z/eL42y / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 12.160437 KDa
SequenceString:
MVNIPKTKNT YCKNKECKKH TLHKVTQYKK GKDSLAAQGK RRYDRKQSGY GGQTKPVFHK KAKTTKKIVL RLQCQSCKHF SQRPIKRCK HFEIGGDKKG KGTSLF

UniProtKB: Large ribosomal subunit protein eL42z/eL42y

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Macromolecule #5: (thale cress) hypothetical protein

MacromoleculeName: (thale cress) hypothetical protein / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 10.356228 KDa
SequenceString:
MTKRTKKAGI IGKYGTRYGA SLRKQIKKME VSQHSKYFCE FCGKYSVKRK VVGIWGCKDC GKVKAGGAYT MNTASAVTVR STIRRLREQ TES

UniProtKB: (thale cress) hypothetical protein

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Macromolecule #6: Large ribosomal subunit protein eL39z/eL39x

MacromoleculeName: Large ribosomal subunit protein eL39z/eL39x / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 6.435789 KDa
SequenceString:
MPSHKSFMIK KKLGKKMRQN RPIPHWIRLR TDNTIRYNAK RRHWRRTKLG F

UniProtKB: Large ribosomal subunit protein eL39z/eL39x

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Macromolecule #7: Large ribosomal subunit protein uL24z

MacromoleculeName: Large ribosomal subunit protein uL24z / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 16.983711 KDa
SequenceString:
MKYNPRVTSS RRKNRKAHFT ASSSERRVIM SSPLSTDLRQ KYNVRSMPIR KDDEVQIVRG TYKGREGKVV QVYRRKWVIH IERITREKV NGTTVNVGIQ PSKVVITKLR LDKDRKSLLE RKAKGRAAAD KEKGTKFTSE DVMQNVD

UniProtKB: Large ribosomal subunit protein uL24z

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Macromolecule #8: Large ribosomal subunit protein eL13z

MacromoleculeName: Large ribosomal subunit protein eL13z / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 23.824018 KDa
SequenceString: MKHNNVIPNG HFKKHWQNYV KTWFNQPARK TRRRIARQKK AVKIFPRPTS GPLRPVVHGQ TLKYNMKVRT GKGFTLEELK AAGIPKKLA PTIGIAVDHR RKNRSLEGLQ TNVQRLKTYK TKLVIFPRRA RKVKAGDSTP EELANATQVQ GDYLPIVREK P TMELVKLT ...String:
MKHNNVIPNG HFKKHWQNYV KTWFNQPARK TRRRIARQKK AVKIFPRPTS GPLRPVVHGQ TLKYNMKVRT GKGFTLEELK AAGIPKKLA PTIGIAVDHR RKNRSLEGLQ TNVQRLKTYK TKLVIFPRRA RKVKAGDSTP EELANATQVQ GDYLPIVREK P TMELVKLT SEMKSFKAFD KIRLERTNKR HAGARAKRAA EAEKEEKK

UniProtKB: Large ribosomal subunit protein eL13z

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Macromolecule #10: Ubiquitin-ribosomal protein eL40z fusion protein

MacromoleculeName: Ubiquitin-ribosomal protein eL40z fusion protein / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 14.763478 KDa
SequenceString:
MQIFVKTLTG KTITLEVESS DTIDNVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLADYN IQKESTLHLV LRLRGGIIEP SLMMLARKY NQDKMICRKC YARLHPRAVN CRKKKCGHSN QLRPKKKIK

UniProtKB: Ubiquitin-ribosomal protein eL40z fusion protein

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Macromolecule #11: Large ribosomal subunit protein eL27x

MacromoleculeName: Large ribosomal subunit protein eL27x / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 15.644693 KDa
SequenceString:
MVKFLKQNKA VILLQGRYAG KKAVIIKSFD DGNRDRPYGH CLVAGLKKYP SKVIRKDSAK KTAKKSRVKC FIKLVNYQHL MPTRYTLDV DLKEVATLDA LQSKDKKVAA LKEAKAKLEE RFKTGKNRWF FTKLRF

UniProtKB: Large ribosomal subunit protein eL27x

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Macromolecule #12: Large ribosomal subunit protein eL14z

MacromoleculeName: Large ribosomal subunit protein eL14z / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 15.536518 KDa
SequenceString:
MGFKRFVEIG RVALVNYGED YGKLVVIVDV VDQNRALVDA PDMERIQMNL KRLSLTDIVI DINRVPKKKV LIEAMEKADV KNKWEKSSW GRKLIVQKRR AALNDFDRFK IMLAKIKRAG IVRQELAKLK REIAA

UniProtKB: Large ribosomal subunit protein eL14z

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Macromolecule #14: Large ribosomal subunit protein eL15y

MacromoleculeName: Large ribosomal subunit protein eL15y / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 24.297225 KDa
SequenceString: MGAYKYVSEL WRKKQSDVMR FLQRVRCWEY RQQPSIVRLV RPTRPDKARR LGYKAKQGFV VYRVRVRRGG RKRPVPKGIV YGKPTNQGV TQLKFQRSKR SVAEERAGRK LGGLRVVNSY WLNEDSTYKY YEIILVDPAH NAVRNDPRIN WICNPVHKHR E LRGLTSEG ...String:
MGAYKYVSEL WRKKQSDVMR FLQRVRCWEY RQQPSIVRLV RPTRPDKARR LGYKAKQGFV VYRVRVRRGG RKRPVPKGIV YGKPTNQGV TQLKFQRSKR SVAEERAGRK LGGLRVVNSY WLNEDSTYKY YEIILVDPAH NAVRNDPRIN WICNPVHKHR E LRGLTSEG KKNRGLRGKG HNNHKNRPSR RATWKKNNSI SLRRYR

UniProtKB: Large ribosomal subunit protein eL15y

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Macromolecule #15: Large ribosomal subunit protein uL15x

MacromoleculeName: Large ribosomal subunit protein uL15x / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 16.498523 KDa
SequenceString:
MTTRFKKNRK KRGHVSAGHG RIGKHRKHPG GRGNAGGMHH HRILFDKYHP GYFGKVGMRY FHKLRNKFFC PIVNLDKLWS LVPEDVKAK STKDNVPLID VTQHGFFKVL GKGHLPENKP FVVKAKLISK TAEKKIKEAG GAVVLTA

UniProtKB: Large ribosomal subunit protein uL15x

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Macromolecule #16: Large ribosomal subunit protein eL22z

MacromoleculeName: Large ribosomal subunit protein eL22z / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 14.045177 KDa
SequenceString:
MSRGGAAVAK GKKKGVSFTI DCSKPVDDKI MEIASLEKFL QERIKVGGKA GALGDSVTIT REKSKITVTA DGQFSKRYLK YLTKKYLKK HNVRDWLRVI AANKDRNLYE LRYFNIAENE GEEED

UniProtKB: Large ribosomal subunit protein eL22z

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Macromolecule #17: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 9.410883 KDa
SequenceString:
MSPIFPLASL PFTQLCCAVV TEMAKSKNHT AHNQSAKAHK NGIKKPRRHR HTPTRGMDPK FLRNQRYARK HNVKSGENAG VEE

UniProtKB: 60S ribosomal protein L29

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Macromolecule #18: Large ribosomal subunit protein uL13w

MacromoleculeName: Large ribosomal subunit protein uL13w / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 23.643324 KDa
SequenceString: MVSGSGICSK RVVVDARHHM LGRLASITAK ELLNGQKVVI VRCEEICLSG GLVRQKMKYM RFLRKRMNTK PSHGPIHFRA PSKIFWRTV RGMIPHKTKR GAAALARLKV YEGVPTPYDK IKRMVIPDAL KVLRLQAGHK YCLLGRLSSE VGWNHYDTIK E LETKRKER ...String:
MVSGSGICSK RVVVDARHHM LGRLASITAK ELLNGQKVVI VRCEEICLSG GLVRQKMKYM RFLRKRMNTK PSHGPIHFRA PSKIFWRTV RGMIPHKTKR GAAALARLKV YEGVPTPYDK IKRMVIPDAL KVLRLQAGHK YCLLGRLSSE VGWNHYDTIK E LETKRKER AHVVYERKKQ LNKLRVKAEK VAEEKLGAQL DILAPVKY

UniProtKB: Large ribosomal subunit protein uL13w

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Macromolecule #19: Large ribosomal subunit protein uL3z

MacromoleculeName: Large ribosomal subunit protein uL3z / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 44.653297 KDa
SequenceString: MSHRKFEHPR HGSLGFLPRK RANRHRGKVK AFPKDDQTKP CKFTAFMGYK AGMTHIVREV EKPGSKLHKK ETCEAVTIIE TPAMVVVGV VAYVKTPRGL RSLNTVWAQH LSEEVRRRFY KNWAKSKKKA FTGYAKQYDS EDGKKGIQAQ LEKMKKYATV I RVLAHTQI ...String:
MSHRKFEHPR HGSLGFLPRK RANRHRGKVK AFPKDDQTKP CKFTAFMGYK AGMTHIVREV EKPGSKLHKK ETCEAVTIIE TPAMVVVGV VAYVKTPRGL RSLNTVWAQH LSEEVRRRFY KNWAKSKKKA FTGYAKQYDS EDGKKGIQAQ LEKMKKYATV I RVLAHTQI RKMKGLKQKK AHMMEIQING GTIAQKVDFA YSFFEKQIPI EAVFQKDEMI DIIGVTKGKG YEGVVTRWGV TR LPRKTHR GLRKVACIGA WHPARVSYTV ARAGQNGYHH RTELNKKIYR LGKVGTEAHT AMTEYDRTEK DVTPMGGFPH YGI VKDDYL MIKGCCVGPK KRVVTLRQSL LTQTSRLALE EIKLKFIDTA SIFGHGRFQT SLEKMRFYNR VTK

UniProtKB: Large ribosomal subunit protein uL3z

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Macromolecule #20: Large ribosomal subunit protein uL22z

MacromoleculeName: Large ribosomal subunit protein uL22z / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 19.937902 KDa
SequenceString:
MVKYSQEPDN ITKSCKARGA DLRVHFKNTR ETAHAIRKLP LNKAKRYLED VIAHKQAIPF TRFCRGVGRT AQAKNRHSNG QGRWPAKSA QFVLDLLKNA ESNAEVKGLD VDALFISHIQ VNQAAKQRRR TYRAHGRINP YMSNPCHIEL ILSEKEEPVK K EPETQLAA KSKKGASS

UniProtKB: Large ribosomal subunit protein uL22z

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Macromolecule #21: Large ribosomal subunit protein eL30x

MacromoleculeName: Large ribosomal subunit protein eL30x / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 12.300389 KDa
SequenceString:
MVAEKKAKKS HEGINSRLAL VMKSGKYTLG YKSVLKSLRS SKGKLILISS NCPPLRRSEI EYYAMLAKVG VHRYNGNNVD LGTACGKYF RVSCLSIVDP GDSDIIKTLP GDQ

UniProtKB: Large ribosomal subunit protein eL30x

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Macromolecule #22: Large ribosomal subunit protein eL18y

MacromoleculeName: Large ribosomal subunit protein eL18y / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 20.972889 KDa
SequenceString:
MGIDLIAGGK SKKTKRTAPK SDDVYLKLTV KLYRFLVRRT NSKFNGVILK RLFMSKVNKA PLSLSRLVEF MTGKEDKIAV LVGTITDDL RVHEIPAMKV TALRFTERAR ARIEKAGGEC LTFDQLALRA PLGQNTVLLR GPKNSREAVK HFGPAPGVPH S HSKPYVRA KGRKFEKARG KRKSRGFKV

UniProtKB: Large ribosomal subunit protein eL18y

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Macromolecule #23: Large ribosomal subunit protein uL4z

MacromoleculeName: Large ribosomal subunit protein uL4z / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 44.783258 KDa
SequenceString: MAAAAARPLV TIQTLDGDMS TDQSSTVVLP DVMTAPVRPD IVNFVHAQIS NNSRQPYAVS KKAGHQTSAE SWGTGRAVSR IPRVPGGGT HRAGQAAFGN MCRGGRMFAP TKIWRRWHRR VNVNMKRHAI VSAIAATAVP ALVMARGHKI ENVPEMPLVV S DSAEAVEK ...String:
MAAAAARPLV TIQTLDGDMS TDQSSTVVLP DVMTAPVRPD IVNFVHAQIS NNSRQPYAVS KKAGHQTSAE SWGTGRAVSR IPRVPGGGT HRAGQAAFGN MCRGGRMFAP TKIWRRWHRR VNVNMKRHAI VSAIAATAVP ALVMARGHKI ENVPEMPLVV S DSAEAVEK TSAAIKVLKQ IGAYDDAEKA KNSIGIRPGK GKMRNRRYIS RKGPLVVYGT EGSKIVKAFR NLPGVELCHV ER LNLLKLA PGGHLGRFVI WTKSAFEKLE SIYGSFEKPS EKKKGYVLPR AKMVNADLAR IINSDEIQSV VNPIKKDAKR AVL KKNPLK NLNVMLKLNP YAKTAKRMSL LAEAQRVKAK KEKLAKKRKT VTKEEALAIK AAGKSWYKTM ISDSDYTEFD NFTK WLGAS Q

UniProtKB: Large ribosomal subunit protein uL4z

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Macromolecule #24: Large ribosomal subunit protein eL19z

MacromoleculeName: Large ribosomal subunit protein eL19z / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 24.395965 KDa
SequenceString: MVSLKIQKRL AASVMKCGKG KVWLDPNESG DISMANSRQN IRKLVKDGFI IRKPTKIHSR SRARALNEAK RKGRHSGYGK RKGTREARL PTKILWMRRM RVLRRFLSKY RESKKIDRHM YHDMYMKVKG NVFKNKRVLM ESIHKMKAEK AREKTLADQF E AKRIKNKA ...String:
MVSLKIQKRL AASVMKCGKG KVWLDPNESG DISMANSRQN IRKLVKDGFI IRKPTKIHSR SRARALNEAK RKGRHSGYGK RKGTREARL PTKILWMRRM RVLRRFLSKY RESKKIDRHM YHDMYMKVKG NVFKNKRVLM ESIHKMKAEK AREKTLADQF E AKRIKNKA SRERKFARRE ERLAQGPGGG ETTTPAGAPQ QPEVTKKKSK K

UniProtKB: Large ribosomal subunit protein eL19z

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Macromolecule #25: Large ribosomal subunit protein eL31x

MacromoleculeName: Large ribosomal subunit protein eL31x / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 13.836154 KDa
SequenceString:
MSEKKGRKEE VITREYTINL HRRLHKCTFK KKAPKAIKEI RKFAEKAMGT KDVRVDVKLN KQIWSKGIRG PPRRIRVRVA RKRNDDEDA KEEFFSLVTV AEIPAEGLSG LGTKVIEEED

UniProtKB: Large ribosomal subunit protein eL31x

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Macromolecule #26: Ribosomal protein L18ae/LX family protein

MacromoleculeName: Ribosomal protein L18ae/LX family protein / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 25.781607 KDa
SequenceString: MISVLELGFL GRCSFIKKKN PSRLYLVEVS TALSLSRRKM GAFRFHQYQV VGRALPTEKD VQPKIYRMKL WATNEVRAKS KFWYFLRKL KKVKKSNGQM LAINEIYEKN PTTIKNFGIW LRYQSRTGYH NMYKEYRDTT LNGAVEQMYT EMASRHRVRF P CIQIIKTA ...String:
MISVLELGFL GRCSFIKKKN PSRLYLVEVS TALSLSRRKM GAFRFHQYQV VGRALPTEKD VQPKIYRMKL WATNEVRAKS KFWYFLRKL KKVKKSNGQM LAINEIYEKN PTTIKNFGIW LRYQSRTGYH NMYKEYRDTT LNGAVEQMYT EMASRHRVRF P CIQIIKTA TVPAKLCKRE STKQFHNSKI KFPLVFRKVR PPSRKLKTTY KANKPNLFM

UniProtKB: Ribosomal protein L18ae/LX family protein

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Macromolecule #27: Large ribosomal subunit protein uL18z

MacromoleculeName: Large ribosomal subunit protein uL18z / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 34.420059 KDa
SequenceString: MVFVKSTKSN AYFKRYQVKF RRRRDGKTDY RARIRLINQD KNKYNTPKYR FVVRFTNKDI VAQIVSASIA GDIVKASAYA HELPQYGLT VGLTNYAAAY CTGLLLARRV LKMLEMDDEY EGNVEATGED FSVEPTDSRR PFRALLDVGL IRTTTGNRVF G ALKGALDG ...String:
MVFVKSTKSN AYFKRYQVKF RRRRDGKTDY RARIRLINQD KNKYNTPKYR FVVRFTNKDI VAQIVSASIA GDIVKASAYA HELPQYGLT VGLTNYAAAY CTGLLLARRV LKMLEMDDEY EGNVEATGED FSVEPTDSRR PFRALLDVGL IRTTTGNRVF G ALKGALDG GLDIPHSDKR FAGFHKENKQ LDAEIHRNYI YGGHVSNYMK LLGEDEPEKL QTHFSAYIKK GVEAESIEEL YK KVHAAIR ADPNPKKTVK PAPKQHKRYN LKKLTYEERK NKLIERVKAL NGAGGDDDDE DDEE

UniProtKB: Large ribosomal subunit protein uL18z

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Macromolecule #28: Large ribosomal subunit protein eL21x/eL21w

MacromoleculeName: Large ribosomal subunit protein eL21x/eL21w / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 18.749914 KDa
SequenceString:
MPAGHGVRAR TRDLFARPFR KKGYIPLSTY LRTFKVGDYV DVKVNGAIHK GMPHKFYHGR TGRIWNVTKR AVGVEVNKQI GNRIIRKRI HVRVEHVQQS RCAEEFKLRK KQNDVLKADA KARGETISTK RQPKGPKPGF MVEGMTLETV TPIPYDVVND L KGGY

UniProtKB: Large ribosomal subunit protein eL21x/eL21w

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Macromolecule #29: Large ribosomal subunit protein eL32z

MacromoleculeName: Large ribosomal subunit protein eL32z / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 15.539284 KDa
SequenceString:
MAVPLLTKKV VKKRSAKFIR PQSDRRITVK ESWRRPKGID SRVRRKFKGV TLMPNVGYGS DKKTRHYLPN GFKKFVVHNT SELELLMMH NRTYCAEIAH NVSTKKRKAI VERASQLDVV VTNRLARLRS QEDE

UniProtKB: Large ribosomal subunit protein eL32z

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Macromolecule #30: Large ribosomal subunit protein uL14x/uL14z/uL14y

MacromoleculeName: Large ribosomal subunit protein uL14x/uL14z/uL14y / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 15.052845 KDa
SequenceString:
MSKRGRGGTS GNKFRMSLGL PVAATVNCAD NTGAKNLYII SVKGIKGRLN RLPSACVGDM VMATVKKGKP DLRKKVLPAV IVRQRKPWR RKDGVFMYFE DNAGVIVNPK GEMKGSAITG PIGKECADLW PRIASAANAI V

UniProtKB: Large ribosomal subunit protein uL14x/uL14z/uL14y

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Macromolecule #31: Large ribosomal subunit protein eL6x

MacromoleculeName: Large ribosomal subunit protein eL6x / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 26.160844 KDa
SequenceString: MPAKQRTPKV NRNPDLIRGV GKYSRSQMYH KRGLWAIKAK NGGVFPRHDA KSKVDAPVEK PPKFYPAEDV KKPLPNRRTA KPTKLRASI TPGTVLIILA GRFKGKRVVF LKQLASGLLL VTGPFKINGV PLRRVNQAYV IGTSTKVDIS GVTLDKFDDK Y FGKVAEKK ...String:
MPAKQRTPKV NRNPDLIRGV GKYSRSQMYH KRGLWAIKAK NGGVFPRHDA KSKVDAPVEK PPKFYPAEDV KKPLPNRRTA KPTKLRASI TPGTVLIILA GRFKGKRVVF LKQLASGLLL VTGPFKINGV PLRRVNQAYV IGTSTKVDIS GVTLDKFDDK Y FGKVAEKK KKKTEGEFFE AEKEEKKEIP QVKKDDQKAV DAALIKAIEA VPELKTYLGA RFSLKQGMKP HELVF

UniProtKB: Large ribosomal subunit protein eL6x

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Macromolecule #32: Large ribosomal subunit protein eL24z

MacromoleculeName: Large ribosomal subunit protein eL24z / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 18.900389 KDa
SequenceString:
MVLKTELCRF SGQKIYPGRG IRFIRSDSQV FLFLNSKCKR YFHNKLKPSK LCWTAMYRKQ HKKDAAQEAV KRRRRATKKP YSRSIVGAT LEVIQKKRAE KPEVRDAARE AALREIKERI KKTKDEKKAK KVEYASKQQK SQVKGNIPKS AAPKAAKMGG G GGRR

UniProtKB: Large ribosomal subunit protein eL24z

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Macromolecule #33: Large ribosomal subunit protein eL33z

MacromoleculeName: Large ribosomal subunit protein eL33z / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 12.823868 KDa
SequenceString:
MKGRQGERVR LYVRGTVLGY KRSKSNQYPN TSLIQIEGVN TQEEVNWYKG KRLAYIYKAK TKKNGSHYRC IWGKVTRPHG NSGVVRSKF TSNLPPKSMG ARVRVFMYPS NI

UniProtKB: Large ribosomal subunit protein eL33z

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Macromolecule #34: Large ribosomal subunit protein uL23y

MacromoleculeName: Large ribosomal subunit protein uL23y / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 17.436879 KDa
SequenceString:
MSPAKVDVTK KADPKAKALK AAKAVKSGQI VKKPAKKIRT KVTFHRPKTL TVPRKPKYPK ISATPRNKLD HYQILKYPLT TESAMKKIE DNNTLVFIVD IRADKKKIKD AVKKMYDIQT KKVNTLIRPD GTKKAYVRLT PDYDALDVAN KIGII

UniProtKB: Large ribosomal subunit protein uL23y

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Macromolecule #35: Large ribosomal subunit protein uL30y

MacromoleculeName: Large ribosomal subunit protein uL30y / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 28.226152 KDa
SequenceString: MVESKVVVPE SVLKKRKREE EWALEKKQNV EAAKKKNAEN RKLIFKRAEQ YSKEYAEKEK ELISLKREAK LKGGFYVDPE AKLLFIIRI RGINAIDPKT KKILQLLRLR QIFNGVFLKV NKATMNMLRR VEPYVTYGFP NLKSVKELIY KRGYGKLNHQ R IALTDNSI ...String:
MVESKVVVPE SVLKKRKREE EWALEKKQNV EAAKKKNAEN RKLIFKRAEQ YSKEYAEKEK ELISLKREAK LKGGFYVDPE AKLLFIIRI RGINAIDPKT KKILQLLRLR QIFNGVFLKV NKATMNMLRR VEPYVTYGFP NLKSVKELIY KRGYGKLNHQ R IALTDNSI VEQALGKHGI ICTEDLIHEI LTVGPHFKEA NNFLWPFQLK APLGGLKKKR NHYVEGGDAG NRENFINELI RR MN

UniProtKB: Large ribosomal subunit protein uL30y

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Macromolecule #36: Large ribosomal subunit protein eL34z

MacromoleculeName: Large ribosomal subunit protein eL34z / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 13.740295 KDa
SequenceString:
MVQRLVYRSR HSYATKSNQH RIVKTPGGKL VYQTTKKRAS GPKCPVTGKR IQGIPHLRPS EYKRSRLSRN RRTVNRAYGG VLSGSAVRE RIIRAFLVEE QKIVKKVLKL QKAKEKVAPK A

UniProtKB: Large ribosomal subunit protein eL34z

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Macromolecule #37: Large ribosomal subunit protein eL8z

MacromoleculeName: Large ribosomal subunit protein eL8z / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 29.191777 KDa
SequenceString: MAPKKGVKVA AKKKTAEKVS NPLFERRPKQ FGIGGALPPK KDLSRYIKWP KSIRLQRQKR ILKQRLKVPP ALNQFTKTLD KNLATSLFK VLLKYRPEDK AAKKERLVKK AQAEAEGKPS ESKKPIVVKY GLNHVTYLIE QNKAQLVVIA HDVDPIELVV W LPALCRKM ...String:
MAPKKGVKVA AKKKTAEKVS NPLFERRPKQ FGIGGALPPK KDLSRYIKWP KSIRLQRQKR ILKQRLKVPP ALNQFTKTLD KNLATSLFK VLLKYRPEDK AAKKERLVKK AQAEAEGKPS ESKKPIVVKY GLNHVTYLIE QNKAQLVVIA HDVDPIELVV W LPALCRKM EVPYCIVKGK SRLGAVVHQK TASCLCLTTV KNEDKLEFSK ILEAIKANFN DKYEEYRKKW GGGIMGSKSQ AK TKAKERV IAKEAAQRMN

UniProtKB: Large ribosomal subunit protein eL8z

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Macromolecule #38: Large ribosomal subunit protein uL29x

MacromoleculeName: Large ribosomal subunit protein uL29x / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 14.215099 KDa
SequenceString:
MARIKVHELR DKSKSDLSTQ LKELKAELAS LRVAKVTGGA PNKLSKIKVV RKSIAQVLTV SSQKQKSALR EAYKNKKLLP LDLRPKKTR AIRRRLTKHQ ASLKTEREKK KDMYFPIRKY AIKV

UniProtKB: Large ribosomal subunit protein uL29x

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Macromolecule #39: Large ribosomal subunit protein uL6z/uL6y

MacromoleculeName: Large ribosomal subunit protein uL6z/uL6y / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 22.059744 KDa
SequenceString: MKTILSSETM DIPDSVTIKV HAKVIEVEGP RGKLVRDFKH LNLDFQLIKD PETGKKKLKI DSWFGTRKTS ASIRTALSHV DNLISGVTR GFRYKMRFVY AHFPINASIG GDGKSIEIRN FLGEKKVRKV EMLDGVTIVR SEKVKDEIVL DGNDIELVSR S CALINQKC ...String:
MKTILSSETM DIPDSVTIKV HAKVIEVEGP RGKLVRDFKH LNLDFQLIKD PETGKKKLKI DSWFGTRKTS ASIRTALSHV DNLISGVTR GFRYKMRFVY AHFPINASIG GDGKSIEIRN FLGEKKVRKV EMLDGVTIVR SEKVKDEIVL DGNDIELVSR S CALINQKC HVKKKDIRKF LDGIYVSEKS KIVEEE

UniProtKB: Large ribosomal subunit protein uL6z/uL6y

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Macromolecule #40: Large ribosomal subunit protein eL36y

MacromoleculeName: Large ribosomal subunit protein eL36y / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 12.713265 KDa
SequenceString:
MTTPQVKTGL FVGLNKGHVV TRRELAPRPR SRKGKTSKRT IFIRNLIKEV AGQAPYEKRI TELLKVGKDK RALKVAKRKL GTHKRAKRK REEMSSVLRK MRSGGGGATE KKK

UniProtKB: Large ribosomal subunit protein eL36y

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Macromolecule #41: Large ribosomal subunit protein uL16y

MacromoleculeName: Large ribosomal subunit protein uL16y / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 24.962348 KDa
SequenceString: MGRRPARCYR QIKGKPYPKS RYCRGVPDPK IRIYDVGMKR KGVDEFPYCV HLVSWEKENV SSEALEAARI ACNKYMVKSA GKDAFHLRI RVHPFHVLRI NKMLSCAGAD RLQTGMRGAF GKALGTCARV AIGQVLLSVR CKDAHGHHAQ EALRRAKFKF P GRQKIIVS ...String:
MGRRPARCYR QIKGKPYPKS RYCRGVPDPK IRIYDVGMKR KGVDEFPYCV HLVSWEKENV SSEALEAARI ACNKYMVKSA GKDAFHLRI RVHPFHVLRI NKMLSCAGAD RLQTGMRGAF GKALGTCARV AIGQVLLSVR CKDAHGHHAQ EALRRAKFKF P GRQKIIVS RKWGFTKFNR ADYTKLRQEK RIVPDGVNAK FLSCHGPLAN RQPGSAFLSA GAQ

UniProtKB: Large ribosomal subunit protein uL16y

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Macromolecule #42: Large ribosomal subunit protein eL37z

MacromoleculeName: Large ribosomal subunit protein eL37z / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 10.844653 KDa
SequenceString:
MGKGTGSFGK RRNKSHTLCV RCGRRSFHIQ KSRCSACAYP AARKRTYNWS VKAIRRKTTG TGRMRYLRNV PRRFKTCFRE GTQATPRNK AAASSS

UniProtKB: Large ribosomal subunit protein eL37z

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Macromolecule #43: Large ribosomal subunit protein uL5z

MacromoleculeName: Large ribosomal subunit protein uL5z / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 20.867227 KDa
SequenceString:
MASEKKLSNP MRDIKVQKLV LNISVGESGD RLTRASKVLE QLSGQTPVFS KARYTVRSFG IRRNEKIACY VTVRGEKAMQ LLESGLKVK EYELLRRNFS DTGCFGFGIQ EHIDLGIKYD PSTGIYGMDF YVVLERPGYR VARRRRCKAR VGIQHRVTKD D AMKWFQVK YEGVILNKSQ NITG

UniProtKB: Large ribosomal subunit protein uL5z

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Macromolecule #44: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 44 / Number of copies: 18 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #45: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 45 / Number of copies: 32 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #46: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 46 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statetissue

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Sample preparation

BufferpH: 7.4
Details: 50 mM Hepes, pH 7.4, 5 mM MgAc, 100 mM KAc, pH 7.5, 1 mM DTT, Complete protease inhibitor
GridModel: Quantifoil / Material: COPPER
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 24.512 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER / Cs: 2.7 mm / Nominal defocus max: 1.2 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 105000
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 115070
Initial angle assignmentType: OTHER
Final angle assignmentType: OTHER

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