EMDB-54286: staphylococcus aureus 70S initiation complex with natural mRNA

Basic information

Entry

Database: EMDB / ID: EMD-54286

• Title: staphylococcus aureus 70S initiation complex with natural mRNA

Sample

• Complex: Staphylococcus aureus 70S ribosome in complex with mRNA and tRNA
  • Protein or peptide: x 47 types
  • RNA: x 6 types

• Keywords: Ribosome / translation initiation / Shine-Dalgarno / anti Shine-Dalgarno / staphylococcus aureus

Function / homology

Function:

large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / RNA binding / zinc ion binding / cytosol / cytoplasm

Domain/homology:

Ribosomal protein L31 type B / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / : / Ribosomal protein S14, type Z / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / : / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / : / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L32p, bacterial type / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S19, bacterial-type / : / Ribosomal protein L6, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S4, bacterial-type / Ribosomal protein L5, bacterial-type / Ribosomal protein S5, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / 30S ribosomal protein S17 / : / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L20 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S14/S29 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L33 / Ribosomal L28 family / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L33 superfamily / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L28/L24 / Ribosomal protein L30, bacterial-type / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / L28p-like / Ribosomal protein L16 / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein L20 / Ribosomal protein S12, bacterial-type / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein L19 superfamily / : / Large ribosomal subunit protein uL24, C-terminal domain / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein L17

Component:

Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein bL31B / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL27 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL33B / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL25 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS15

• Biological species: Staphylococcaceae bacterium (bacteria) / Staphylococcus aureus (bacteria) / Escherichia coli (E. coli)
• Method: single particle reconstruction / cryo EM / Resolution: 2.9 Å
• Authors: Bahena R / Klaholz B / Marzi S

Funding support

France, 2 items

Organization	Grant number	Country
Agence Nationale de la Recherche (ANR)	ANR-21-CE12-0030-01	France
Agence Nationale de la Recherche (ANR)	ANR-23-CE12-0041-01	France

• Citation: Journal: To Be Published; Title: staphylococcus aureus 70S initiation complex with natural spa mRNA; Authors: Bahena R / Klaholz B

History

Deposition	Jul 7, 2025	-
Header (metadata) release	Jul 22, 2026	-
Map release	Jul 22, 2026	-
Update	Jul 22, 2026	-
Current status	Jul 22, 2026	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_54286.map.gz / Format: CCP4 / Size: 476.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 0.9 Å

Density

Contour Level	By AUTHOR: 0.15
Minimum - Maximum	-0.4657444 - 1.2568352
Average (Standard dev.)	-0.0025711565 (±0.0466313)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 500 500 500 Spacing 500 500 500
Cell	A=B=C: 450.0 Å α=β=γ: 90.0 °

Supplemental data

Additional map: Sharp map for visualization

• File: emd_54286_additional_1.map
• Annotation: Sharp map for visualization

Half map: #2

• File: emd_54286_half_map_1.map

Half map: #1

• File: emd_54286_half_map_2.map

Sample components

Entire : Staphylococcus aureus 70S ribosome in complex with mRNA and tRNA

• Entire: Name: Staphylococcus aureus 70S ribosome in complex with mRNA and tRNA

Components

• Complex: Staphylococcus aureus 70S ribosome in complex with mRNA and tRNA
  • Protein or peptide: Large ribosomal subunit protein bL33B
  • Protein or peptide: Large ribosomal subunit protein bL34
  • Protein or peptide: Large ribosomal subunit protein bL35
  • Protein or peptide: Large ribosomal subunit protein bL36
  • Protein or peptide: Large ribosomal subunit protein bL31B
  • RNA: 16S rRNA
  • Protein or peptide: Small ribosomal subunit protein uS2
  • Protein or peptide: Small ribosomal subunit protein uS3
  • Protein or peptide: Small ribosomal subunit protein uS4
  • Protein or peptide: Small ribosomal subunit protein uS5
  • Protein or peptide: Small ribosomal subunit protein bS6
  • Protein or peptide: Small ribosomal subunit protein uS7
  • Protein or peptide: Small ribosomal subunit protein uS8
  • Protein or peptide: Small ribosomal subunit protein uS9
  • Protein or peptide: Large ribosomal subunit protein uL13
  • Protein or peptide: Small ribosomal subunit protein uS11
  • Protein or peptide: Small ribosomal subunit protein uS12
  • Protein or peptide: Small ribosomal subunit protein uS13
  • Protein or peptide: Small ribosomal subunit protein uS14B
  • Protein or peptide: Small ribosomal subunit protein uS15
  • Protein or peptide: Small ribosomal subunit protein bS16
  • Protein or peptide: Small ribosomal subunit protein uS17
  • Protein or peptide: Small ribosomal subunit protein bS18
  • Protein or peptide: Small ribosomal subunit protein uS19
  • Protein or peptide: Small ribosomal subunit protein bS20
  • Protein or peptide: Large ribosomal subunit protein uL14
  • RNA: spa mRNA
  • RNA: A-site tRNA-Lys
  • RNA: P-site tRNA-Met
  • RNA: 23S rRNA
  • RNA: 5S
  • Protein or peptide: Large ribosomal subunit protein uL2
  • Protein or peptide: Large ribosomal subunit protein uL3
  • Protein or peptide: Large ribosomal subunit protein uL4
  • Protein or peptide: Large ribosomal subunit protein uL5
  • Protein or peptide: Large ribosomal subunit protein uL6
  • Protein or peptide: Small ribosomal subunit protein uS10
  • Protein or peptide: Large ribosomal subunit protein uL15
  • Protein or peptide: Large ribosomal subunit protein uL16
  • Protein or peptide: Large ribosomal subunit protein bL17
  • Protein or peptide: Large ribosomal subunit protein uL18
  • Protein or peptide: Large ribosomal subunit protein bL19
  • Protein or peptide: Large ribosomal subunit protein bL20
  • Protein or peptide: Large ribosomal subunit protein bL21
  • Protein or peptide: Large ribosomal subunit protein uL22
  • Protein or peptide: Large ribosomal subunit protein uL23
  • Protein or peptide: Large ribosomal subunit protein uL24
  • Protein or peptide: Large ribosomal subunit protein bL25
  • Protein or peptide: Large ribosomal subunit protein bL27
  • Protein or peptide: Large ribosomal subunit protein bL28
  • Protein or peptide: Large ribosomal subunit protein uL29
  • Protein or peptide: Large ribosomal subunit protein uL30
  • Protein or peptide: Large ribosomal subunit protein bL32

Supramolecule #1: Staphylococcus aureus 70S ribosome in complex with mRNA and tRNA

• Supramolecule: Name: Staphylococcus aureus 70S ribosome in complex with mRNA and tRNA; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#53
• Source (natural): Organism: Staphylococcaceae bacterium (bacteria) / Strain: HG001

Macromolecule #1: Large ribosomal subunit protein bL33B

• Macromolecule: Name: Large ribosomal subunit protein bL33B / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 5.885783 KDa

Sequence

String:

MRVNVTLACT ECGDRNYITT KNKRNNPERV EMKKFCSREN KQTLHRETK

UniProtKB: Large ribosomal subunit protein bL33B

Macromolecule #2: Large ribosomal subunit protein bL34

• Macromolecule: Name: Large ribosomal subunit protein bL34 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 5.454642 KDa

Sequence

String:

MVKRTYQPNK RKHSKVHGFR KRMSTKNGRK VLARRRRKGR KVLSA

UniProtKB: Large ribosomal subunit protein bL34

Macromolecule #3: Large ribosomal subunit protein bL35

• Macromolecule: Name: Large ribosomal subunit protein bL35 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 7.722368 KDa

Sequence

String:

MPKMKTHRGA AKRVKRTASG QLKRSRAFTS HLFANKSTKQ KRQLRKARLV SKSDMKRVKQ LLAYKK

UniProtKB: Large ribosomal subunit protein bL35

Macromolecule #4: Large ribosomal subunit protein bL36

• Macromolecule: Name: Large ribosomal subunit protein bL36 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 4.318422 KDa

Sequence

String:

MKVRPSVKPI CEKCKVIKRK GKVMVICENP KHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

Macromolecule #5: Large ribosomal subunit protein bL31B

• Macromolecule: Name: Large ribosomal subunit protein bL31B / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 9.737912 KDa

Sequence

String:

MKQGIHPEYH QVIFLDTTTN FKFLSGSTKT SSEMMEWEDG KEYPVIRLDI SSDSHPFYTG RQKFAAADGR VERFNKKFGL KSNN

UniProtKB: Large ribosomal subunit protein bL31B

Macromolecule #7: Small ribosomal subunit protein uS2

• Macromolecule: Name: Small ribosomal subunit protein uS2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 29.136369 KDa

Sequence

String:

MAVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKKV DEAYNFLKQV SEDGGQVLFV GTKKQAQESV KSEAERAGQ FYINQRWLGG LLTNYKTISK RIKRISEIEK MEEDGLFEVL PKKEVVELKK EYDRLIKFLG GIRDMKSMPQ A LFVVDPRK ERNAIAEARK LNIPIVGIVD TNCDPDEIDY VIPANDDIAR AVKLLTAKMA DAILEGQQGV SNEEVAAEQN ID LDEKEKS EETEATEE

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #8: Small ribosomal subunit protein uS3

• Macromolecule: Name: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 24.143867 KDa

Sequence

String:

MGQKINPIGL RVGIIRDWEA KWYAEKDFAS LLHEDLKIRK FIDNELKEAS VSHVEIERAA NRINIAIHTG KPGMVIGKGG SEIEKLRNK LNALTDKKVH INVIEIKKVD LDARLVAENI ARQLENRASF RRVQKQAITR AMKLGAKGIK TQVSGRLGGA D IARAEQYS EGTVPLHTLR ADIDYAHAEA DTTYGKLGVK VWIYRGEVLP TKNTSGGGK

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #9: Small ribosomal subunit protein uS4

• Macromolecule: Name: Small ribosomal subunit protein uS4 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 23.051416 KDa

Sequence

String:

MARFRGSNWK KSRRLGISLS GTGKELEKRP YAPGQHGPNQ RKKLSEYGLQ LREKQKLRYL YGMTERQFRN TFDIAGKKFG VHGENFMIL LASRLDAVVY SLGLARTRRQ ARQLVNHGHI LVDGKRVDIP SYSVKPGQTI SVREKSQKLN IIVESVEINN F VPEYLNFD ADSLTGTFVR LPERSELPAE INEQLIVEYY SR

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #10: Small ribosomal subunit protein uS5

• Macromolecule: Name: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 17.770512 KDa

Sequence

String:

MARREEETKE FEERVVTINR VAKVVKGGRR FRFTALVVVG DKNGRVGFGT GKAQEVPEAI KKAVEAAKKD LVVVPRVEGT TPHTITGRY GSGSVFMKPA APGTGVIAGG PVRAVLELAG ITDILSKSLG SNTPINMVRA TIDGLQNLKN AEDVAKLRGK T VEELYN

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #11: Small ribosomal subunit protein bS6

• Macromolecule: Name: Small ribosomal subunit protein bS6 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 11.613146 KDa

Sequence

String:

MRTYEVMYIV RPNIEEDAKK ALVERFNGIL ATEGAEVLEA KDWGKRRLAY EINDFKDGFY NIVRVKSDNN KATDEFQRLA KISDDIIRY MVIREDEDK

UniProtKB: Small ribosomal subunit protein bS6

Macromolecule #12: Small ribosomal subunit protein uS7

• Macromolecule: Name: Small ribosomal subunit protein uS7 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 17.826555 KDa

Sequence

String:

MPRKGSVPKR DVLPDPIHNS KLVTKLINKI MLDGKRGTAQ RILYSAFDLV EQRSGRDALE VFEEAINNIM PVLEVKARRV GGSNYQVPV EVRPERRTTL GLRWLVNYAR LRGEKTMEDR LANEILDAAN NTGGAVKKRE DTHKMAEANK AFAHYRW

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #13: Small ribosomal subunit protein uS8

• Macromolecule: Name: Small ribosomal subunit protein uS8 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 14.854315 KDa

Sequence

String:

MTMTDPIADM LTRVRNANMV RHEKLELPAS NIKKEIAEIL KSEGFIKNVE YVEDDKQGVL RLFLKYGQND ERVITGLKRI SKPGLRVYA KASEMPKVLN GLGIALVSTS EGVITDKEAR KRNVGGEIIA YVW

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #14: Small ribosomal subunit protein uS9

• Macromolecule: Name: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 14.856987 KDa

Sequence

String:

MTLAQVEYRG TGRRKNSVAR VRLVPGEGNI TVNNRDVREY LPFESLILDL NQPFDVTETK GNYDVLVNVH GGGFTGQAQA IRHGIARAL LEADPEYRGS LKRAGLLTRD PRMKERKKPG LKAARRSPQF SKR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #15: Large ribosomal subunit protein uL13

• Macromolecule: Name: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 16.359427 KDa

Sequence

String:

MRQTFMANES NIERKWYVID AEGQTLGRLS SEVASILRGK NKVTYTPHVD TGDYVIVINA SKIEFTGNKE TDKVYYRHSN HPGGIKSIT AGELRRTNPE RLIENSIKGM LPSTRLGEKQ GKKLFVYGGA EHPHAAQQPE NYELRG

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #16: Small ribosomal subunit protein uS11

• Macromolecule: Name: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 13.879964 KDa

Sequence

String:

MARKQVSRKR RVKKNIENGV AHIRSTFNNT IVTITDEFGN ALSWSSAGAL GFKGSKKSTP FAAQMASETA SKSAMEHGLK TVEVTVKGP GPGRESAIRA LQSAGLEVTA IRDVTPVPHN GCRPPKKRRV

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #17: Small ribosomal subunit protein uS12

• Macromolecule: Name: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 15.376897 KDa

Sequence

String:

MPTINQLVRK PRQSKIKKSD SPALNKGFNS KKKKFTDLNS PQKRGVCTRV GTMTPRKPNS ALRKYARVRL SNNIEINAYI PGIGHNLQE HSVVLVRGGR VRDLPGVRYH IVRGALDTSG VDGRRQGRSL YGTKKPKN

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #18: Small ribosomal subunit protein uS13

• Macromolecule: Name: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 13.747919 KDa

Sequence

String:

MARIAGVDIP REKRVVISLT YIYGIGTSTA QKILEEANVS ADTRVKDLTD DELGRIREVV DGYKVEGDLR RETNLNIKRL MEISSYRGI RHRRGLPVRG QKTKNNARTR KGPVKTVANK KK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #19: Small ribosomal subunit protein uS14B

• Macromolecule: Name: Small ribosomal subunit protein uS14B / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 7.317769 KDa

Sequence

String:

MAKTSMVAKQ QKKQKYAVRE YTRCERCGRP HSVYRKFKLC RICFRELAYK GQIPGVRKAS W

UniProtKB: Small ribosomal subunit protein uS14B

Macromolecule #20: Small ribosomal subunit protein uS15

• Macromolecule: Name: Small ribosomal subunit protein uS15 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 10.63433 KDa

Sequence

String:

MAISQERKNE IIKEYRVHET DTGSPEVQIA VLTAEINAVN EHLRTHKKDH HSRRGLLKMV GRRRHLLNYL RSKDIQRYRE LIKSLGIRR

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #21: Small ribosomal subunit protein bS16

• Macromolecule: Name: Small ribosomal subunit protein bS16 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 10.253886 KDa

Sequence

String:

MAVKIRLTRL GSKRNPFYRI VVADARSPRD GRIIEQIGTY NPTSANAPEI KVDEALALKW LNDGAKPTDT VHNILSKEGI MKKFDEQKK AK

UniProtKB: Small ribosomal subunit protein bS16

Macromolecule #22: Small ribosomal subunit protein uS17

• Macromolecule: Name: Small ribosomal subunit protein uS17 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 10.196888 KDa

Sequence

String:

MSERNDRKVY VGKVVSDKMD KTITVLVETY KTHKLYGKRV KYSKKYKTHD ENNSAKLGDI VKIQETRPLS ATKRFRLVEI VEESVII

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #23: Small ribosomal subunit protein bS18

• Macromolecule: Name: Small ribosomal subunit protein bS18 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 9.332018 KDa

Sequence

String:

MAGGPRRGGR RRKKVCYFTA NGITHIDYKD TELLKRFISE RGKILPRRVT GTSAKYQRML TTAIKRSRHM ALLPYVKEEQ

UniProtKB: Small ribosomal subunit protein bS18

Macromolecule #24: Small ribosomal subunit protein uS19

• Macromolecule: Name: Small ribosomal subunit protein uS19 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 10.639309 KDa

Sequence

String:

MARSIKKGPF VDEHLMKKVE AQEGSEKKQV IKTWSRRSTI FPNFIGHTFA VYDGRKHVPV YVTEDMVGHK LGEFAPTRTF KGHVADDKK TRR

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #25: Small ribosomal subunit protein bS20

• Macromolecule: Name: Small ribosomal subunit protein bS20 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 9.012446 KDa

Sequence

String:

MANIKSAIKR VKTTEKAEAR NISQKSAMRT AVKNAKTAVS NNADNKNELV SLAVKLVDKA AQSNLIHSNK ADRIKSQLMT ASK

UniProtKB: Small ribosomal subunit protein bS20

Macromolecule #26: Large ribosomal subunit protein uL14

• Macromolecule: Name: Large ribosomal subunit protein uL14 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 13.157342 KDa

Sequence

String:

MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKNATPGG VVKKGDVVKA VIVRTKSGVR RNDGSYIKFD ENACVIIRD DKGPRGTRIF GPVARELREG NFMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #32: Large ribosomal subunit protein uL2

• Macromolecule: Name: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 30.217164 KDa

Sequence

String:

MAIKKYKPIT NGRRNMTSLD FAEITKTTPE KSLLKPLPKK AGRNNQGKLT VRHHGGGHKR QYRVIDFKRN KDGINAKVDS IQYDPNRSA NIALVVYADG EKRYIIAPKG LEVGQIVESG AEADIKVGNA LPLQNIPVGT VVHNIELKPG KGGQIARSAG A SAQVLGKE GKYVLIRLRS GEVRMILSTC RATIGQVGNL QHELVNVGKA GRSRWKGIRP TVRGSVMNPN DHPHGGGEGR AP IGRPSPM SPWGKPTLGK KTRRGKKSSD KLIVRGRKKK

UniProtKB: Large ribosomal subunit protein uL2

Macromolecule #33: Large ribosomal subunit protein uL3

• Macromolecule: Name: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 23.760256 KDa

Sequence

String:

MTKGILGRKI GMTQVFGENG ELIPVTVVEA KENVVLQKKT VEVDGYNAIQ VGFEDKKAYK KDAKSNKYAN KPAEGHAKKA DAAPKRFIR EFRNVDVDAY EVGQEVSVDT FVAGDVIDVT GVSKGKGFQG AIKRHGQSRG PMSHGSHFHR APGSVGMASD A SRVFKGQK MPGRMGGNTV TVQNLEVVQV DTENKVILVK GNVPGPKKGL VEIRTSIKKG NK

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #34: Large ribosomal subunit protein uL4

• Macromolecule: Name: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 22.495697 KDa

Sequence

String:

MANYDVLKLD GTKSGSIELS DAVFGIEPNN SVLFEAINLQ RASLRQGTHA VKNRSAVSGG GRKPWKQKGT GRARQGTIRA PQWRGGGIV FGPTPRSYAY KMPKKMRRLA LRSALSFKAQ ENGLTVVDAF NFEAPKTKEF KNVLSTLEQP KKVLVVTENE D VNVELSAR NIPGVQVTTA QGLNVLDITN ADSLVITEAA AKKVEEVLG

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #35: Large ribosomal subunit protein uL5

• Macromolecule: Name: Large ribosomal subunit protein uL5 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 20.296637 KDa

Sequence

String:

MNRLKEKFNT EVTENLMKKF NYSSVMEVPK IDKIVVNMGV GDAVQNSKVL DNAVEELELI TGQKPLVTKA KKSIATFRLR EGMPIGAKV TLRGERMYEF LDKLISVSLP RVRDFQGVSK KAFDGRGNYT LGVKEQLIFP EIDYDKVSKV RGMDIVIVTT A NTDEEARE LLANFGMPFR K

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #36: Large ribosomal subunit protein uL6

• Macromolecule: Name: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 19.81858 KDa

Sequence

String:

MSRVGKKIID IPSDVTVTFD GNHVTVKGPK GELSRTLNER MTFKQEENTI EVVRPSDSKE DRTNHGTTRA LLNNMVQGVS QGYVKVLEL VGVGYRAQMQ GKDLILNVGY SHPVEIKAEE NITFSVEKNT VVKVEGISKE QVGALASNIR SVRPPEPYKG K GIRYQGEY VRRKEGKTGK

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #37: Small ribosomal subunit protein uS10

• Macromolecule: Name: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 11.598503 KDa

Sequence

String:

MAKQKIRIRL KAYDHRVIDQ SAEKIVETAK RSGADVSGPI PLPTEKSVYT IIRAVHKYKD SREQFEQRTH KRLIDIVNPT PKTVDALMG LNLPSGVDIE IKL

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #38: Large ribosomal subunit protein uL15

• Macromolecule: Name: Large ribosomal subunit protein uL15 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 15.62889 KDa

Sequence

String:

MKLHELKPAE GSRKERNRVG RGVATGNGKT SGRGHKGQKA RSGGGVRPGF EGGQLPLFRR LPKRGFTNIN RKEYAIVNLD QLNKFEDGT EVTPALLVES GVVKNEKSGI KILGNGSLDK KLTVKAHKFS ASAAEAIDAK GGAHEVI

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #39: Large ribosomal subunit protein uL16

• Macromolecule: Name: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 16.274049 KDa

Sequence

String:

MLLPKRVKYR RQHRPKTTGR SKGGNYVTFG EFGLQATTTS WITSRQIESA RIAMTRYMKR GGKVWIKIFP HTPYTKKPLE VRMGAGKGA VEGWIAVVKP GRILFEVAGV SEEVAREALR LASHKLPVKT KFVKREELGG ETNES

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #40: Large ribosomal subunit protein bL17

• Macromolecule: Name: Large ribosomal subunit protein bL17 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 13.771773 KDa

Sequence

String:

MGYRKLGRTS DQRKAMLRDL ATSLIISERI ETTEARAKEV RSVVEKLITL GKKGDLASRR NAAKTLRNVE ILNEDETTQT ALQKLFGEI AERYTERQGG YTRILKQGPR RGDGAESVII ELV

UniProtKB: Large ribosomal subunit protein bL17

Macromolecule #41: Large ribosomal subunit protein uL18

• Macromolecule: Name: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 13.080083 KDa

Sequence

String:

MISKIDKNKV RLKRHARVRT NLSGTAEKPR LNVYRSNKHI YAQIIDDNKG VTLAQASSKD SIAATTATKV ELATKVGEAI AKKAADKGI KEIVFDRGGY LYHGRVKALA EAARESGLEF

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #42: Large ribosomal subunit protein bL19

• Macromolecule: Name: Large ribosomal subunit protein bL19 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 13.392771 KDa

Sequence

String:

MTNHKLIEAV TKSQLRTDLP SFRPGDTLRV HVRIIEGTRE RIQVFEGVVI KRRGGGVSET FTVRKISSGV GVERTFPLHT PKIEKIEVK RRGKVRRAKL YYLRSLRGKA ARIQEIR

UniProtKB: Large ribosomal subunit protein bL19

Macromolecule #43: Large ribosomal subunit protein bL20

• Macromolecule: Name: Large ribosomal subunit protein bL20 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 13.720295 KDa

Sequence

String:

MPRVKGGTVT RARRKKTIKL AKGYFGSKHT LYKVAKQQVM KSGQYAFRDR RQRKRDFRKL WITRINAAAR QHEMSYSRLM NGLKKAGID INRKMLSEIA ISDEKAFAQL VTKAKDALK

UniProtKB: Large ribosomal subunit protein bL20

Macromolecule #44: Large ribosomal subunit protein bL21

• Macromolecule: Name: Large ribosomal subunit protein bL21 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 11.354081 KDa

Sequence

String:

MFAIIETGGK QIKVEEGQEI FVEKLDVNEG DTFTFDKVLF VGGDSVKVGA PTVEGATVTA TVNKQGRGKK ITVFTYKRRK NSKRKKGHR QPYTKLTIDK INA

UniProtKB: Large ribosomal subunit protein bL21

Macromolecule #45: Large ribosomal subunit protein uL22

• Macromolecule: Name: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 12.857922 KDa

Sequence

String:

MEAKAVARTI RIAPRKVRLV LDLIRGKNAA EAIAILKLTN KASSPVIEKV LMSALANAEH NYDMNTDELV VKEAYANEGP TLKRFRPRA QGRASAINKR TSHITIVVSD GKEEAKEA

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #46: Large ribosomal subunit protein uL23

• Macromolecule: Name: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 10.625398 KDa

Sequence

String:

MEARDILKRP VITEKSSEAM AEDKYTFDVD TRVNKTQVKM AVEEIFNVKV ASVNIMNYKP KKKRMGRYQG YTNKRRKAIV TLKEGSIDL FN

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #47: Large ribosomal subunit protein uL24

• Macromolecule: Name: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 11.574545 KDa

Sequence

String:

MHIKKGDNVK VIAGKDKGKE GKVIATLPKK DRVVVEGVNI MKKHQKPTQL NPAQGILETE AAIHVSNVQL LDPKTNEPTR VGYKFVDGK KVRIAKKSGE EIKSNN

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #48: Large ribosomal subunit protein bL25

• Macromolecule: Name: Large ribosomal subunit protein bL25 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 23.840637 KDa

Sequence

String:

MASLKSIIRQ GKQTRSDLKQ LRKSGKVPAV VYGYGTKNVS VKVDEVEFIK VIREVGRNGV IELGVGSKTI KVMVADYQFD PLKNQITHI DFLAINMSEE RTVEVPVQLV GEAVGAKEGG VVEQPLFNLE VTATPDNIPE AIEVDITELN INDSLTVADV K VTSDFKIE NDSAESVVTV VAPTEEPTEE EIEAMEGEQQ TEEPEVVGES KEDEEKTEE

UniProtKB: Large ribosomal subunit protein bL25

Macromolecule #49: Large ribosomal subunit protein bL27

• Macromolecule: Name: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 10.334798 KDa

Sequence

String:

MLKLNLQFFA SKKGVSSTKN GRDSESKRLG AKRADGQFVT GGSILYRQRG TKIYPGENVG RGGDDTLFAK IDGVVKFERK GRDKKQVSV YAVAE

UniProtKB: Large ribosomal subunit protein bL27

Macromolecule #50: Large ribosomal subunit protein bL28

• Macromolecule: Name: Large ribosomal subunit protein bL28 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 6.995289 KDa

Sequence

String:

MGKQCFVTGR KASTGNRRSH ALNSTKRRWN ANLQKVRILV DGKPKKVWVS ARALKSGKVT RV

UniProtKB: Large ribosomal subunit protein bL28

Macromolecule #51: Large ribosomal subunit protein uL29

• Macromolecule: Name: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 8.105266 KDa

Sequence

String:

MKAKEIRDLT TSEIEEQIKS SKEELFNLRF QLATGQLEET ARIRTVRKTI ARLKTVARER EIEQSKANQ

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #52: Large ribosomal subunit protein uL30

• Macromolecule: Name: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 6.565683 KDa

Sequence

String:

MAKLQITLTR SVIGRPETQR KTVEALGLKK TNSSVVVEDN PAIRGQINKV KHLVTVEEK

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #53: Large ribosomal subunit protein bL32

• Macromolecule: Name: Large ribosomal subunit protein bL32 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 6.657803 KDa

Sequence

String:

MAVPKRRTSK TRKNKRRTHF KISVPGMTEC PNCGREYKLS HRVCKNCGSY NGEEVAAK

UniProtKB: Large ribosomal subunit protein bL32

Macromolecule #6: 16S rRNA

• Macromolecule: Name: 16S rRNA / type: rna / ID: 6 / Number of copies: 1
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 502.3245 KDa

Sequence

String:

UUUAUGGAGA GUUUGAUCCU GGCUCAGGAU GAACGCUGGC GGCGUGCCUA AUACAUGCAA GUCGAGCGAA CGGACGAGAA GCUUGCUUC UCUGAUGUUA GCGGCGGACG GGUGAGUAAC ACGUGGAUAA CCUACCUAUA AGACUGGGAU AACUUCGGGA A ACCGUAGC UAAUACCGGA UAAUAUUUUG AACCGCAUGG UUCAAAAGUG AAAGACGGUC UUGCUGUCAC UUAUAGAUGG AU CCGCGCU GCAUUAGCUA GUUGGUAAGG UAACGGCUUA CCAAGGCAAC GAUGCAUAGC CGACCUGAGA GGGUGAUCGG CCA CACUGG AACUGAGACA CGGUCCAGAC UCCUACGGGA GGCAGCAGUA GGGAAUCUUC CGCAAUGGGC GAAAGCCUGA CGGA GCAAC GCCGCGUGAG UGAUGAAGGU CUUCGGAUCG UAAAACUCUG UUAUUAGGGA AGAACAUAUG UGUAAGUAAC UGUGC ACAU CUUGACGGUA CCUAAUCAGA AAGCCACGGC UAACUACGUG CCAGCAGCCG CGGUAAUACG UAGGUGGCAA GCGUUA UCC GGAAUUAUUG GGCGUAAAGC GCGCGUAGGC GGUUUUUUAA GUCUGAUGUG AAAGCCCACG GCUCAACCGU GGAGGGU CA UUGGAAACUG GAAAACUUGA GUGCAGAAGA GGAAAGUGGA AUUCCAUGUG UAGCGGUGAA AUGCGCAGAG AUAUGGAG G AACACCAGUG GCGAAGGCGA CUUUCUGGUC UGUAACUGAC GCUGAUGUGC GAAAGCGUGG GGAUCAAACA GGAUUAGAU ACCCUGGUAG UCCACGCCGU AAACGAUGAG UGCUAAGUGU UAGGGGGUUU CCCGCCCCUU AGUGCUGCAG CUAACGCAUU AAGCACUCC GCCUGGGGAG UACGACCGCA AGGUUGAAAC UCAAAGGAAU UGACGGGGAC CCGCACAAGC GGUGGAGCAU G UGGUUUAA UUCGAAGCAA CGCGAAGAAC CUUACCAAAU CUUGACAUCC UUUGACAACU CUAGAGAUAG AGCCUUCCCC UU CGGGGGA CAAAGUGACA GGUGGUGCAU GGUUGUCGUC AGCUCGUGUC GUGAGAUGUU GGGUUAAGUC CCGCAACGAG CGC AACCCU UAAGCUUAGU UGCCAUCAUU AAGUUGGGCA CUCUAAGUUG ACUGCCGGUG ACAAACCGGA GGAAGGUGGG GAUG ACGUC AAAUCAUCAU GCCCCUUAUG AUUUGGGCUA CACACGUGCU ACAAUGGACA AUACAAAGGG CAGCGAAACC GCGAG GUCA AGCAAAUCCC AUAAAGUUGU UCUCAGUUCG GAUUGUAGUC UGCAACUCGA CUACAUGAAG CUGGAAUCGC UAGUAA UCG UAGAUCAGCA UGCUACGGUG AAUACGUUCC CGGGUCUUGU ACACACCGCC CGUCACACCA CGAGAGUUUG UAACACC CG AAGCCGGUGG AGUAACCUUU UAGGAGCUAG CCGUCGAAGG UGGGACAAAU GAUUGGGGUG AAGUCGUAAC AAGGUAGC C GUAUCGGAAG GUGCGGCUGG AUCACCUCCU UU

Macromolecule #27: spa mRNA

• Macromolecule: Name: spa mRNA / type: rna / ID: 27 / Number of copies: 1
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 7.430433 KDa

Sequence

String:

CAGGGGGUAU UAAUUUGGUA UAA

Macromolecule #28: A-site tRNA-Lys

• Macromolecule: Name: A-site tRNA-Lys / type: rna / ID: 28 / Number of copies: 1
• Source (natural): Organism: Escherichia coli (E. coli)
• Molecular weight: Theoretical: 24.377426 KDa

Sequence

String:

GGGUCGUUAG CUCAGUUGGU AGAGCAGUUG ACUUUUAAUC AAUUGGCCGC AGGUUCGAAU CCUGCACGAC CCACCA

Macromolecule #29: P-site tRNA-Met

• Macromolecule: Name: P-site tRNA-Met / type: rna / ID: 29 / Number of copies: 1
• Source (natural): Organism: Escherichia coli (E. coli)
• Molecular weight: Theoretical: 24.497602 KDa

Sequence

String:

GCGGGGUGGA GCAGCCUGGU AGCUCGUCGG GCUCAUAACC CGAAGGUCGU CGGUUCAAAU CCGGCCCCCG CAACCA

Macromolecule #30: 23S rRNA

• Macromolecule: Name: 23S rRNA / type: rna / ID: 30 / Number of copies: 1
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 943.81175 KDa

Sequence

String:

UUAAGUUAUU AAGGGCGCAC GGUGGAUGCC UUGGCACUAG AAGCCGAUGA AGGACGUUAC UAACGACGAU AUGCUUUGGG GAGCUGUAA GUAAGCUUUG AUCCAGAGAU UUCCGAAUGG GGAAACCCAG CAUGAGUUAU GUCAUGUUAU CGAUAUGUGA A UACAUAGC AUAUCAGAAG GCACACCCGG AGAACUGAAA CAUCUUAGUA CCCGGAGGAA GAGAAAGAAA AUUCGAUUCC CU UAGUAGC GGCGAGCGAA AUGGGAAGAG CCCAAACCAA CAAGCUUGCU UGUUGGGGUU GUAGGACACU CUAUACGGAG UUA CAAAGG ACGACAUUAG ACGAAUCAUC UGGAAAGAUG AAUCAAAGAA GGUAAUAAUC CUGUAGUCGA AAAUGUUGUC UCUC UUGAG UGGAUCCUGA GUACGACGGA GCACGUGAAA UUCCGUCGGA AUCUGGGAGG ACCAUCUCCU AAGGCUAAAU ACUCU CUAG UGACCGAUAG UGAACCAGUA CCGUGAGGGA AAGGUGAAAA GCACCCCGGA AGGGGAGUGA AAUAGAACCU GAAACC GUG UGCUUACAAG UAGUCAGAGC CCGUUAAUGG GUGAUGGCGU GCCUUUUGUA GAAUGAACCG GCGAGUUACG AUUUGAU GC AAGGUUAAGC AGUAAAUGUG GAGCCGUAGC GAAAGCGAGU CUGAAUAGGG CGUUUAGUAU UUGGUCGUAG ACCCGAAA C CAGGUGAUCU ACCCUUGGUC AGGUUGAAGU UCAGGUAACA CUGAAUGGAG GACCGAACCG ACUUACGUUG AAAAGUGAG CGGAUGAACU GAGGGUAGCG GAGAAAUUCC AAUCGAACCU GGAGAUAGCU GGUUCUCUCC GAAAUAGCUU UAGGGCUAGC CUCAAGUGA UGAUUAUUGG AGGUAGAGCA CUGUUUGGAC GAGGGGCCCC UCGGGUACCG AAUUCAGACA AACUCCGAAU G CCAAUUAA UUUAACUUGG GAGUCAGAAC AUGGGUGAUA AGGUCCGUGU UCGAAAGGGA AACAGCCCAG ACCACCAGCU AA GGUCCCA AAAUAUAUGU UAAGUGGAAA AGGAUGUGGC GUUGCCCAGA CAACUAGGAU GUUGGCUUAG AAGCAGCCAU CAU UUAAAG AGUGCGUAAU AGCUCACUAG UCGAGUGACA CUGCGCCGAA AAUGUACCGG GGCUAAACAU AUUACCGAAG CUGU GGAUU GUCCUUUGGA CAAUGGUAGG AGAGCGUUCU AAGGGCGUUG AAGCAUGAUC GUAAGGACAU GUGGAGCGCU UAGAA GUGA GAAUGCCGGU GUGAGUCGCG AAAGACGGGU GAGAAUCCCG UCCACCGAUU GACUAAGGUU UCCAGAGGAA GGCUCG UCC GCUCUGGGUU AGUCGGGUCC UAAGCUGAGG CCGACAGGCG UAGGCGAUGG AUAACAGGUU GAUAUUCCUG UACCACC UA UAAUCGUUUU AAUCGAUGGG GGGACGCAGU AGGAUAGGCG AAGCGUGCGA UUGGAUUGCA CGUCUAAGCA GUAAGGCU G AGUAUUAGGC AAAUCCGGUA CUCGUUAAGG CUGAGCUGUG AUGGGGAGAA GACAUUGAGU CUUCGAGUCG UUGAUUUCA CACUGCCGAG AAAAGCCUCU AGAUAGAAAA UAGGUGCCCG UACCGCAAAC CGACACAGGU AGUCAAGAUG AGAAUUCUAA GGUGAGCGA GCGAACUCUC GUUAAGGAAC UCGGCAAAAU GACCCCGUAA CUUCGGGAGA AGGGGUGCUC UUUAGGGUUA A CGCCCAGA AGAGCCGCAG UGAAUAGGCC CAAGCGACUG UUUAUCAAAA ACACAGGUCU CUGCUAAACC GUAAGGUGAU GU AUAGGGG CUGACGCCUG CCCGGUGCUG GAAGGUUAAG AGGAGUGGUU AGCUUCUGCG AAGCUACGAA UCGAAGCCCC AGU AAACGG CGGCCGUAAC UAUAACGGUC CUAAGGUAGC GAAAUUCCUU GUCGGGUAAG UUCCGACCCG CACGAAAGGC GUAA CGAUU UGGGCACUGU CUCAACGAGA GACUCGGUGA AAUCAUAGUA CCUGUGAAGA UGCAGGUUAC CCGCGACAGG ACGGA AAGA CCCCGUGGAG CUUUACUGUA GCCUGAUAUU GAAAUUCGGC ACAGCUUGUA CAGGAUAGGU GGAGCCUUUG AAACGU GAG CGCUAGCUUA CGUGGAGGCG CUGGUGGGAU ACUACCCUAG CUGUGUUGGC UUUCUAACCC GCACCACUUA UCGUGGU GG GAGACAGUGU CAGGCGGGCA GUUUGACUGG GGCGGUCGCC UCCUAAAAGG UAACGGAGGC GCUCAAAGGU UCCCUCAG A AUGGUUGGAA AUCAUUCAUA GAGUGUAAAG GCAUAAGGGA GCUUGACUGC GAGACCUACA AGUCGAGCAG GGUCGAAAG ACGGACUUAG UGAUCCGGUG GUUCCGCAUG GAAGGGCCAU CGCUCAACGG AUAAAAGCUA CCCCGGGGAU AACAGGCUUA UCUCCCCCA AGAGUUCACA UCGACGGGGA GGUUUGGCAC CUCGAUGUCG GCUCAUCGCA UCCUGGGGCU GUAGUCGGUC C CAAGGGUU GGGCUGUUCG CCCAUUAAAG CGGUACGCGA GCUGGGUUCA GAACGUCGUG AGACAGUUCG GUCCCUAUCC GU CGUGGGC GUAGGAAAUU UGAGAGGAGC UGUCCUUAGU ACGAGAGGAC CGGGAUGGAC AUACCUCUGG UGUACCAGUU GUC GUGCCA ACGGCAUAGC UGGGUAGCUA UGUGUGGACG GGAUAAGUGC UGAAAGCAUC UAAGCAUGAA GCCCCCCUCA AGAU GAGAU UUCCCAACUU CGGUUAUAAG AUCCCUCAAA GAUGAUGAGG UUAAUAGGUU CGAGGUGGAA GCAUGGUGAC AUGUG GAGC UGACGAAUAC UAAUCGAUCG AAGACUUAAU

Macromolecule #31: 5S

• Macromolecule: Name: 5S / type: rna / ID: 31 / Number of copies: 1
• Source (natural): Organism: Staphylococcus aureus (bacteria)
• Molecular weight: Theoretical: 36.692801 KDa

Sequence

String:

UCUGGUGACU AUAGCAAGGA GGUCACACCU GUUCCCAUGC CGAACACAGA AGUUAAGGUC UUUAGCGACG AUGGUAGCCA ACUUACGUU CCGCUAGAGU AGAACGUUGC CAGGC

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: 3D array

Sample preparation

• Concentration: 1 mg/mL
• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: TFS GLACIOS
• Image recording: Film or detector model: GATAN K2 BASE (4k x 4k) / Average electron dose: 30.0 e/Ų
• Electron beam: Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm

Image processing

• CTF correction: Software - Name: cryoSPARC (ver. 4.7) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

6yef

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.7) / Number images used: 21621
• Initial angle assignment: Type: ANGULAR RECONSTITUTION / Software - Name: cryoSPARC (ver. 4.7)
• Final angle assignment: Type: ANGULAR RECONSTITUTION / Software - Name: cryoSPARC (ver. 4.7)